(Circular) (Six-base) MAPSORT of: pFN21ASDA0049.seq Check: 497 from: 1 to: 7627 pFN21ASDA0049 7627 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3542 3542 Size: 7627 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5437 278 Size: 53 83 186 936 3901 2468 Fragments arranged by size: 3901 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3386 4970 1937 Size: 1449 1584 4594 Fragments arranged by size: 4594 1584 1449 Acc65I G'GTAC_C Cuts at: 4948 4948 Size: 7627 AclI AA'CG_TT Cuts at: 1563 5754 6127 1563 Size: 4191 373 3063 Fragments arranged by size: 4191 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2604 2813 3164 4086 4335 4545 4782 4859 Size: 972 209 351 922 249 210 237 77 Cuts at: 4859 4868 5690 7074 7342 1632 Size: 9 822 1384 268 1917 Fragments arranged by size: 1917 1384 972 922 822 351 268 249 237 210 209 77 9 AfeI AGC'GCT Cuts at: 2223 2223 Size: 7627 AflII C'TTAA_G Cuts at: 820 1017 2225 820 Size: 197 1208 6222 Fragments arranged by size: 6222 1208 197 AflIII A'CryG_T Cuts at: 1203 6526 7503 1203 Size: 5323 977 1327 Fragments arranged by size: 5323 1327 977 AgeI A'CCGG_T Cuts at: 6458 6458 Size: 7627 AhdI GACnn_n'nnGTC Cuts at: 3703 6356 3703 Size: 2653 4974 AleI CACnn'nnGTG Cuts at: 1166 3767 1166 Size: 2601 5026 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7627 AlwNI CAG_nnn'CTG Cuts at: 3700 4435 4568 4742 5370 6942 3700 Size: 735 133 174 628 1572 4385 Fragments arranged by size: 4385 1572 735 628 174 133 ApaLI G'TGCA_C Cuts at: 5684 6840 5684 Size: 1156 6471 ApoI r'AATT_y Cuts at: 1493 2064 3006 3019 3157 4936 5162 5314 7423 Size: 571 942 13 138 1779 226 152 2109 Cuts at: 7423 1493 Size: 1697 Fragments arranged by size: 2109 1779 1697 942 571 226 152 138 13 AseI AT'TA_AT Cuts at: 160 1032 6181 160 Size: 872 5149 1606 Fragments arranged by size: 5149 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7627 AvaI C'yCGr_G Cuts at: 1870 1943 3432 4952 1870 Size: 73 1489 1520 4545 Fragments arranged by size: 4545 1520 1489 73 BamHI G'GATC_C Cuts at: 2410 4957 5324 2410 Size: 2547 367 4713 Fragments arranged by size: 4713 2547 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2277 4644 4948 6403 618 Size: 325 217 627 490 2367 304 1455 1842 Fragments arranged by size: 2367 1842 1455 627 490 325 304 217 BanII G_rGCy'C Cuts at: 1391 2819 3044 3482 3663 4173 4496 4590 4946 Size: 1428 225 438 181 510 323 94 356 Cuts at: 4946 1391 Size: 4072 Fragments arranged by size: 4072 1428 510 438 356 323 225 181 94 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2405 2852 3951 928 Size: 971 506 447 1099 4604 Fragments arranged by size: 4604 1099 971 506 447 BbvCI CC'TCA_GC Cuts at: 4110 4353 4110 Size: 243 7384 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2406 2440 5818 5852 1903 Size: 34 469 34 3378 34 3678 Fragments arranged by size: 3678 3378 469 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2200 5519 6729 2200 Size: 3319 1210 3098 Fragments arranged by size: 3319 3098 1210 BclI T'GATC_A Cuts at: 1548 3504 4570 1548 Size: 1956 1066 4605 Fragments arranged by size: 4605 1956 1066 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4784 6238 136 Size: 107 122 71 631 605 3112 1454 1525 Fragments arranged by size: 3112 1525 1454 631 605 122 107 71 BglII A'GATC_T Cuts at: 7622 7622 Size: 7627 BlpI GC'TnA_GC Cuts at: 5044 5044 Size: 7627 Bme1580I G_kGCm'C Cuts at: 1790 2280 3756 4449 5688 6844 1790 Size: 490 1476 693 1239 1156 2573 Fragments arranged by size: 2573 1476 1239 1156 693 490 BmgBI CAC'GTC Cuts at: 1318 2785 1318 Size: 1467 6160 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2300 3955 5358 Size: 416 53 83 399 24 883 1655 1403 Cuts at: 5358 6316 442 Size: 958 1753 Fragments arranged by size: 1753 1655 1403 958 883 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7627 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2516 2555 2873 3020 3062 3229 4322 4395 Size: 1277 39 318 147 42 167 1093 73 Cuts at: 4395 4689 6287 1239 Size: 294 1598 2579 Fragments arranged by size: 2579 1598 1277 1093 318 294 167 147 73 42 39 Bpu10I CC'TnA_GC Cuts at: 4110 4353 4110 Size: 243 7384 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2445 3242 3308 3496 4624 5752 6617 6915 2445 Size: 797 66 188 1128 1128 865 298 3157 Fragments arranged by size: 3157 1128 1128 865 797 298 188 66 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2797 6290 882 Size: 617 1298 3493 2219 Fragments arranged by size: 3493 2219 1298 617 BsaAI yAC'GTr Cuts at: 493 1204 4924 5418 493 Size: 711 3720 494 2702 Fragments arranged by size: 3720 2702 711 494 BsaBI GATnn'nnATC Cuts at: 7621 7621 Size: 7627 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3400 5434 5816 6451 Size: 53 83 186 936 1867 2034 382 635 Cuts at: 6451 275 Size: 1451 Fragments arranged by size: 2034 1867 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6060 6458 6732 6879 7290 6060 Size: 398 274 147 411 6397 Fragments arranged by size: 6397 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3368 3398 4662 4692 1178 Size: 30 165 30 1965 30 1264 30 4113 Fragments arranged by size: 4113 1965 1264 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2809 2844 3783 4106 4145 4148 4151 4160 Size: 1623 35 939 323 39 3 3 9 Cuts at: 4160 4169 4172 4175 4596 1186 Size: 9 3 3 421 4217 Fragments arranged by size: 4217 1623 939 421 323 39 35 9 9 3 3 3 3 BseYI C'CCAG_C Cuts at: 3367 4337 4358 4727 6830 7326 3367 Size: 970 21 369 2103 496 3668 Fragments arranged by size: 3668 2103 970 496 369 21 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2441 3159 3264 3463 4728 1163 Size: 573 705 718 105 199 1265 4062 Fragments arranged by size: 4062 1265 718 705 573 199 105 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5077 5838 6866 1540 Size: 275 186 3076 761 1028 2301 Fragments arranged by size: 3076 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3663 4173 4401 4496 5688 5773 6844 3663 Size: 510 228 95 1192 85 1071 4446 Fragments arranged by size: 4446 1192 1071 510 228 95 85 BsmI GAATG_Cn' Cuts at: 5138 5231 5138 Size: 93 7534 BsmBI CGTCTCn'nnnn_ Cuts at: 2492 2492 Size: 7627 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2280 2819 3044 3482 3663 3756 4173 Size: 399 490 539 225 438 181 93 417 Cuts at: 4173 4401 4449 4496 4590 4946 5688 5773 6844 Size: 228 48 47 94 356 742 85 1071 Cuts at: 6844 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 539 490 438 417 399 356 228 225 181 94 93 85 48 47 BspEI T'CCGG_A Cuts at: 7290 7290 Size: 7627 BspHI T'CATG_A Cuts at: 5516 7246 5516 Size: 1730 5897 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3542 3708 4855 4982 844 Size: 2698 166 1147 127 3489 Fragments arranged by size: 3489 2698 1147 166 127 BsrBI CCG'CTC Cuts at: 1394 1823 5514 1394 Size: 429 3691 3507 Fragments arranged by size: 3691 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3360 3918 4662 6122 6296 65 Size: 1183 2112 558 744 1460 174 1396 Fragments arranged by size: 2112 1460 1396 1183 744 558 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3329 6271 6458 1700 Size: 110 1519 2942 187 2869 Fragments arranged by size: 2942 2869 1519 187 110 BsrGI T'GTAC_A Cuts at: 96 4272 4504 96 Size: 4176 232 3219 Fragments arranged by size: 4176 3219 232 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7627 BssSI C'ACGA_G Cuts at: 5687 6699 5687 Size: 1012 6615 BstAPI GCAn_nnn'nTGC Cuts at: 794 2679 2935 794 Size: 1885 256 5486 Fragments arranged by size: 5486 1885 256 BstBI TT'CG_AA Cuts at: 2007 6431 2007 Size: 4424 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7627 BstXI CCAn_nnnn'nTGG Cuts at: 3425 3425 Size: 7627 BstYI r'GATC_y Cuts at: 1972 2410 3457 3679 4957 5324 5709 5726 7178 Size: 438 1047 222 1278 367 385 17 1452 Cuts at: 7178 7264 7276 7622 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1278 1047 438 385 367 346 222 86 17 12 BstZ17I GTA'TAC Cuts at: 3387 3387 Size: 7627 BtgI C'CryG_G Cuts at: 513 1065 2022 3028 513 Size: 552 957 1006 5112 Fragments arranged by size: 5112 1006 957 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6888 Fragments arranged by size: 6888 683 56 BtsI GCAGTG_nn' Cuts at: 2424 4481 4740 5143 5937 5957 2424 Size: 2057 259 403 794 20 4094 Fragments arranged by size: 4094 2057 794 403 259 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7627 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7592 DraI TTT'AAA Cuts at: 2056 3871 4931 2056 Size: 1815 1060 4752 Fragments arranged by size: 4752 1815 1060 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6634 809 Size: 507 5318 1802 Fragments arranged by size: 5318 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2767 3331 5074 Size: 54 1425 50 275 18 937 564 1743 Cuts at: 5074 5963 7592 8 Size: 889 1629 43 Fragments arranged by size: 1743 1629 1425 937 889 564 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5074 1537 Size: 275 3262 4090 Fragments arranged by size: 4090 3262 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2178 2371 3468 3843 4619 5557 1353 Size: 825 193 1097 375 776 938 3423 Fragments arranged by size: 3423 1097 938 825 776 375 193 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6214 6588 6734 387 Size: 1064 138 4625 374 146 1280 Fragments arranged by size: 4625 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3661 4171 4494 3661 Size: 510 323 6794 Fragments arranged by size: 6794 510 323 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2516 2555 2604 2813 2873 3020 3062 Size: 393 884 39 49 209 60 147 42 Cuts at: 3062 3164 3229 4086 4322 4335 4395 4545 4689 Size: 102 65 857 236 13 60 150 144 Cuts at: 4689 4782 4859 4868 5690 6287 7074 7342 1239 Size: 93 77 9 822 597 787 268 1524 Fragments arranged by size: 1524 884 857 822 787 597 393 268 236 209 150 147 144 102 93 77 65 60 60 49 42 39 13 9 EcoNI CCTnn'n_nnAGG Cuts at: 2171 2171 Size: 7627 EcoO109I rG'GnC_Cy Cuts at: 3697 3957 4717 3697 Size: 260 760 6607 Fragments arranged by size: 6607 760 260 EcoRI G'AATT_C Cuts at: 3019 4936 5314 3019 Size: 1917 378 5332 Fragments arranged by size: 5332 1917 378 EcoRV GAT'ATC Cuts at: 2113 2113 Size: 7627 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2240 3847 4091 2240 Size: 1607 244 5776 Fragments arranged by size: 5776 1607 244 FseI GG_CCGG'CC Cuts at: 3333 3333 Size: 7627 FspI TGC'GCA Cuts at: 6133 6133 Size: 7627 HaeII r_GCGC'y Cuts at: 1678 2225 6774 1678 Size: 547 4549 2531 Fragments arranged by size: 4549 2531 547 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3689 3721 4774 4806 6268 Size: 32 135 32 2117 32 1053 32 1462 Cuts at: 6268 6300 6342 6374 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2117 1462 1053 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2521 4971 5217 669 Size: 988 281 583 2450 246 3079 Fragments arranged by size: 3079 2450 988 583 281 246 HindIII A'AGCT_T Cuts at: 748 2071 2537 2561 748 Size: 1323 466 24 5814 Fragments arranged by size: 5814 1323 466 24 HpaI GTT'AAC Cuts at: 2521 5217 2521 Size: 2696 4931 KpnI G_GTAC'C Cuts at: 4952 4952 Size: 7627 MfeI C'AATT_G Cuts at: 5226 5226 Size: 7627 MluI A'CGCG_T Cuts at: 7503 7503 Size: 7627 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3699 3723 6741 6925 1305 Size: 2394 24 3018 184 2007 Fragments arranged by size: 3018 2394 2007 184 24 MscI TGG'CCA Cuts at: 10 64 1489 1832 2769 10 Size: 54 1425 343 937 4868 Fragments arranged by size: 4868 1425 937 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3767 4512 4650 5585 5944 6103 518 Size: 648 2601 745 138 935 359 159 2042 Fragments arranged by size: 2601 2042 935 745 648 359 159 138 MspA1I CmG'CkG Cuts at: 1606 2658 4352 4822 5043 5720 6868 7113 1606 Size: 1052 1694 470 221 677 1148 245 2120 Fragments arranged by size: 2120 1694 1148 1052 677 470 245 221 NaeI GCC'GGC Cuts at: 3331 3331 Size: 7627 NcoI C'CATG_G Cuts at: 513 1065 2022 3028 513 Size: 552 957 1006 5112 Fragments arranged by size: 5112 1006 957 552 NdeI CA'TA_TG Cuts at: 387 2764 387 Size: 2377 5250 NgoMIV G'CCGG_C Cuts at: 3329 3329 Size: 7627 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7627 NotI GC'GGCC_GC Cuts at: 5074 5074 Size: 7627 NspI r_CATG'y Cuts at: 1132 1229 3790 4985 6530 1132 Size: 97 2561 1195 1545 2229 Fragments arranged by size: 2561 2229 1545 1195 97 PasI CC'CwG_GG Cuts at: 1348 4258 1348 Size: 2910 4717 PciI A'CATG_T Cuts at: 6526 6526 Size: 7627 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7627 PmeI GTTT'AAAC Cuts at: 4931 4931 Size: 7627 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5696 5728 7209 7241 5696 Size: 32 1481 32 6082 Fragments arranged by size: 6082 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3697 3957 3697 Size: 260 7367 PshAI GACnn'nnGTC Cuts at: 1613 4036 1613 Size: 2423 5204 PsiI TTA'TAA Cuts at: 5197 5197 Size: 7627 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2535 2567 2535 Size: 32 7595 PstI C_TGCA'G Cuts at: 830 2259 2419 4866 4979 830 Size: 1429 160 2447 113 3478 Fragments arranged by size: 3478 2447 1429 160 113 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7627 PvuII CAG'CTG Cuts at: 2658 4352 4822 2658 Size: 1694 470 5463 Fragments arranged by size: 5463 1694 470 SacI G_AGCT'C Cuts at: 3663 4173 4496 3663 Size: 510 323 6794 Fragments arranged by size: 6794 510 323 SalI G'TCGA_C Cuts at: 1936 4969 1936 Size: 3033 4594 SanDI GG'GwC_CC Cuts at: 3957 3957 Size: 7627 SapI GCTCTTCn'nnn_ Cuts at: 4619 4619 Size: 7627 SbfI CC_TGCA'GG Cuts at: 4866 4979 4866 Size: 113 7514 ScaI AGT'ACT Cuts at: 2846 5875 7587 2846 Size: 3029 1712 2886 Fragments arranged by size: 3029 2886 1712 SfcI C'TryA_G Cuts at: 826 1045 2255 2415 4862 4975 6110 6791 6982 Size: 219 1210 160 2447 113 1135 681 191 Cuts at: 6982 826 Size: 1471 Fragments arranged by size: 2447 1471 1210 1135 681 219 191 160 113 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7627 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7627 SmaI CCC'GGG Cuts at: 1872 4954 1872 Size: 3082 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2225 2460 3257 3323 3511 4639 Size: 197 926 282 235 797 66 188 1128 Cuts at: 4639 5731 6632 6894 820 Size: 1092 901 262 1553 Fragments arranged by size: 1553 1128 1092 926 901 797 282 262 235 197 188 66 SnaBI TAC'GTA Cuts at: 493 4924 5418 493 Size: 4431 494 2702 Fragments arranged by size: 4431 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7627 SphI G_CATG'C Cuts at: 1132 4985 1132 Size: 3853 3774 SspI AAT'ATT Cuts at: 5 52 5551 5 Size: 47 5499 2081 Fragments arranged by size: 5499 2081 47 StuI AGG'CCT Cuts at: 1517 4299 1517 Size: 2782 4845 StyI C'CwwG_G Cuts at: 513 1065 2022 2583 3028 3372 3996 5066 513 Size: 552 957 561 445 344 624 1070 3074 Fragments arranged by size: 3074 1070 957 624 561 552 445 344 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5654 5671 5824 6009 6454 325 Size: 1255 4074 17 153 185 445 1498 Fragments arranged by size: 4074 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2844 4272 4504 Size: 274 80 33 51 1443 867 1428 232 Cuts at: 4504 5873 7585 96 Size: 1369 1712 138 Fragments arranged by size: 1712 1443 1428 1369 867 274 232 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2683 2715 4662 4694 7587 7619 2683 Size: 32 1947 32 2893 32 2691 Fragments arranged by size: 2893 2691 1947 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7627 XbaI T'CTAG_A Cuts at: 4963 4963 Size: 7627 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4255 4255 Size: 7627 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7627 XmaI C'CCGG_G Cuts at: 1870 4952 1870 Size: 3082 4545 XmnI GAAnn'nnTTC Cuts at: 3552 4881 5756 3552 Size: 1329 875 5423 Fragments arranged by size: 5423 1329 875 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5435 276 Size: 53 83 186 936 3901 2468 Fragments arranged by size: 3901 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI Bsu36I DraIII FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspOMI PspXI RsrII SacII SexAI SfiI SfoI SrfI SwaI