(Circular) (Six-base) MAPSORT of: pFN21ASDA0043.seq Check: 8692 from: 1 to: 6907 pFN21ASDA0043 6907 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2964 2964 Size: 6907 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2783 4717 278 Size: 53 83 186 936 1247 1934 2468 Fragments arranged by size: 2468 1934 1247 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4250 1937 Size: 2313 4594 Acc65I G'GTAC_C Cuts at: 4228 4228 Size: 6907 AclI AA'CG_TT Cuts at: 1563 5034 5407 1563 Size: 3471 373 3063 Fragments arranged by size: 3471 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3452 3692 4970 6354 6622 1632 Size: 1820 240 1278 1384 268 1917 Fragments arranged by size: 1917 1820 1384 1278 268 240 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6710 AflIII A'CryG_T Cuts at: 1203 3240 5806 6783 1203 Size: 2037 2566 977 1327 Fragments arranged by size: 2566 2037 1327 977 AgeI A'CCGG_T Cuts at: 5738 5738 Size: 6907 AhdI GACnn_n'nnGTC Cuts at: 5636 5636 Size: 6907 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6907 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3711 1184 Size: 2527 4380 AlwNI CAG_nnn'CTG Cuts at: 4650 6222 4650 Size: 1572 5335 ApaI G_GGCC'C Cuts at: 2075 4118 2075 Size: 2043 4864 ApaLI G'TGCA_C Cuts at: 3440 3809 4964 6120 3440 Size: 369 1155 1156 4227 Fragments arranged by size: 4227 1156 1155 369 ApoI r'AATT_y Cuts at: 1493 2415 4216 4442 4594 6703 1493 Size: 922 1801 226 152 2109 1697 Fragments arranged by size: 2109 1801 1697 922 226 152 AseI AT'TA_AT Cuts at: 160 1032 5461 160 Size: 872 4429 1606 Fragments arranged by size: 4429 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6907 AvaI C'yCGr_G Cuts at: 1870 1943 2068 3785 3825 4232 1870 Size: 73 125 1717 40 407 4545 Fragments arranged by size: 4545 1717 407 125 73 40 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2607 2640 2607 Size: 33 6874 BamHI G'GATC_C Cuts at: 2055 4237 4604 2055 Size: 2182 367 4358 Fragments arranged by size: 4358 2182 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2914 3286 4228 5683 618 Size: 325 217 627 1127 372 942 1455 1842 Fragments arranged by size: 1842 1455 1127 942 627 372 325 217 BanII G_rGCy'C Cuts at: 1391 2075 2512 3279 3362 3834 4118 4226 1391 Size: 684 437 767 83 472 284 108 4072 Fragments arranged by size: 4072 767 684 472 437 284 108 83 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2171 928 Size: 971 272 5664 Fragments arranged by size: 5664 971 272 BbvCI CC'TCA_GC Cuts at: 3095 3095 Size: 6907 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3969 4003 5098 5132 1903 Size: 34 2032 34 1095 34 3678 Fragments arranged by size: 3678 2032 1095 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4799 6009 4799 Size: 1210 5697 BclI T'GATC_A Cuts at: 1548 1548 Size: 6907 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3308 3590 3608 Size: 107 122 71 631 605 1636 282 18 Cuts at: 3608 3638 5518 136 Size: 30 1880 1525 Fragments arranged by size: 1880 1636 1525 631 605 282 122 107 71 30 18 BglII A'GATC_T Cuts at: 6902 6902 Size: 6907 BlpI GC'TnA_GC Cuts at: 3612 3761 4058 4099 4127 4324 3612 Size: 149 297 41 28 197 6195 Fragments arranged by size: 6195 297 197 149 41 28 Bme1580I G_kGCm'C Cuts at: 1790 2075 2583 2919 3444 3813 4118 4968 6124 Size: 285 508 336 525 369 305 850 1156 Cuts at: 6124 1790 Size: 2573 Fragments arranged by size: 2573 1156 850 525 508 369 336 305 285 BmgBI CAC'GTC Cuts at: 1318 2649 3241 1318 Size: 1331 592 4984 Fragments arranged by size: 4984 1331 592 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2651 3467 4638 Size: 416 53 83 399 24 1234 816 1171 Cuts at: 4638 5596 442 Size: 958 1753 Fragments arranged by size: 1753 1234 1171 958 816 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 4004 1056 Size: 2948 3959 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3376 3376 Size: 6907 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2921 3071 3218 3929 4150 5567 1239 Size: 1682 150 147 711 221 1417 2579 Fragments arranged by size: 2579 1682 1417 711 221 150 147 Bpu10I CC'TnA_GC Cuts at: 3095 3839 4005 3095 Size: 744 166 5997 Fragments arranged by size: 5997 744 166 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5032 5897 6195 5032 Size: 865 298 5744 Fragments arranged by size: 5744 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2104 5570 882 Size: 617 605 3466 2219 Fragments arranged by size: 3466 2219 617 605 BsaAI yAC'GTr Cuts at: 493 1204 4204 4698 493 Size: 711 3000 494 2702 Fragments arranged by size: 3000 2702 711 494 BsaBI GATnn'nnATC Cuts at: 6901 6901 Size: 6907 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2600 2780 4714 5096 Size: 53 83 186 936 1067 180 1934 382 Cuts at: 5096 5731 275 Size: 635 1451 Fragments arranged by size: 1934 1451 1067 936 635 382 186 180 83 53 BsaWI w'CCGG_w Cuts at: 5340 5738 6012 6159 6570 5340 Size: 398 274 147 411 5677 Fragments arranged by size: 5677 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2755 2785 1178 Size: 30 165 30 1352 30 5300 Fragments arranged by size: 5300 1352 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2673 3368 3404 3407 3734 3737 4010 4132 Size: 1487 695 36 3 327 3 273 122 Cuts at: 4132 1186 Size: 3961 Fragments arranged by size: 3961 1487 695 327 273 122 36 3 3 BseYI C'CCAG_C Cuts at: 2108 2557 2665 2919 3796 6110 6606 2108 Size: 449 108 254 877 2314 496 2409 Fragments arranged by size: 2409 2314 877 496 449 254 108 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3041 1163 Size: 573 1305 5029 Fragments arranged by size: 5029 1305 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2122 2541 3660 4357 5118 6146 Size: 275 186 121 419 1119 697 761 1028 Cuts at: 6146 1540 Size: 2301 Fragments arranged by size: 2301 1119 1028 761 697 419 275 186 121 BsiHKAI G_wGCw'C Cuts at: 2955 3362 3444 3813 4968 5053 6124 2955 Size: 407 82 369 1155 85 1071 3738 Fragments arranged by size: 3738 1155 1071 407 369 85 82 BsmI GAATG_Cn' Cuts at: 4418 4511 4418 Size: 93 6814 BsmBI CGTCTCn'nnnn_ Cuts at: 2596 2596 Size: 6907 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2075 2512 2583 2919 2955 3279 3362 Size: 399 285 437 71 336 36 324 83 Cuts at: 3362 3444 3813 3834 4118 4226 4968 5053 6124 Size: 82 369 21 284 108 742 85 1071 Cuts at: 6124 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 437 399 369 336 324 285 284 108 85 83 82 71 36 21 BspEI T'CCGG_A Cuts at: 6570 6570 Size: 6907 BspHI T'CATG_A Cuts at: 4796 6526 4796 Size: 1730 5177 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2238 2510 2964 4262 844 Size: 1394 272 454 1298 3489 Fragments arranged by size: 3489 1394 1298 454 272 BsrBI CCG'CTC Cuts at: 1394 1823 3397 4794 1394 Size: 429 1574 1397 3507 Fragments arranged by size: 3507 1574 1397 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5402 5576 65 Size: 1183 4154 174 1396 Fragments arranged by size: 4154 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2497 3289 3659 3777 5551 5738 1700 Size: 110 687 792 370 118 1774 187 2869 Fragments arranged by size: 2869 1774 792 687 370 187 118 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6907 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6907 BssSI C'ACGA_G Cuts at: 3206 3443 4967 5979 3206 Size: 237 1524 1012 4134 Fragments arranged by size: 4134 1524 1012 237 BstAPI GCAn_nnn'nTGC Cuts at: 794 2146 794 Size: 1352 5555 BstBI TT'CG_AA Cuts at: 2007 5711 2007 Size: 3704 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6907 BstXI CCAn_nnnn'nTGG Cuts at: 2848 3409 2848 Size: 561 6346 BstYI r'GATC_y Cuts at: 1972 2055 4237 4604 4989 5006 6458 6544 6556 Size: 83 2182 367 385 17 1452 86 12 Cuts at: 6556 6902 1972 Size: 346 1977 Fragments arranged by size: 2182 1977 1452 385 367 346 86 83 17 12 Bsu36I CC'TnA_GG Cuts at: 2978 4108 2978 Size: 1130 5777 BtgI C'CryG_G Cuts at: 513 1065 2049 2208 2706 3219 3279 3306 3315 Size: 552 984 159 498 513 60 27 9 Cuts at: 3315 3654 513 Size: 339 3766 Fragments arranged by size: 3766 984 552 513 498 339 159 60 27 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2593 487 Size: 683 56 1367 4801 Fragments arranged by size: 4801 1367 683 56 BtsI GCAGTG_nn' Cuts at: 2634 2790 3355 4195 4423 5217 5237 2634 Size: 156 565 840 228 794 20 4304 Fragments arranged by size: 4304 840 794 565 228 156 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6907 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6872 DraI TTT'AAA Cuts at: 4211 4211 Size: 6907 DraIII CAC_nnn'GTG Cuts at: 3559 3559 Size: 6907 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5914 809 Size: 507 4598 1802 Fragments arranged by size: 4598 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2119 2538 2784 Size: 54 1425 50 275 18 289 419 246 Cuts at: 2784 2869 3121 3216 3453 3657 4354 5243 6872 Size: 85 252 95 237 204 697 889 1629 Cuts at: 6872 8 Size: 43 Fragments arranged by size: 1629 1425 889 697 419 289 275 252 246 237 204 95 85 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2119 2538 3657 4354 1537 Size: 275 307 419 1119 697 4090 Fragments arranged by size: 4090 1119 697 419 307 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2279 3367 3567 3711 4837 1353 Size: 926 1088 200 144 1126 3423 Fragments arranged by size: 3423 1126 1088 926 200 144 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3428 3585 5494 5868 6014 387 Size: 1064 138 1839 157 1909 374 146 1280 Fragments arranged by size: 1909 1839 1280 1064 374 157 146 138 EcoICRI GAG'CTC Cuts at: 3360 3360 Size: 6907 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2921 3071 3218 3452 3692 3929 4150 Size: 393 1289 150 147 234 240 237 221 Cuts at: 4150 4970 5567 6354 6622 1239 Size: 820 597 787 268 1524 Fragments arranged by size: 1524 1289 820 787 597 393 268 240 237 234 221 150 147 EcoNI CCTnn'n_nnAGG Cuts at: 3464 3838 3464 Size: 374 6533 EcoO109I rG'GnC_Cy Cuts at: 2072 2896 3046 3469 3727 4114 2072 Size: 824 150 423 258 387 4865 Fragments arranged by size: 4865 824 423 387 258 150 EcoRI G'AATT_C Cuts at: 4216 4594 4216 Size: 378 6529 FspI TGC'GCA Cuts at: 5413 5413 Size: 6907 HaeII r_GCGC'y Cuts at: 1678 3296 3333 3757 6054 1678 Size: 1618 37 424 2297 2531 Fragments arranged by size: 2531 2297 1618 424 37 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2975 3007 5548 5580 5622 Size: 32 135 32 1403 32 2541 32 42 Cuts at: 5622 5654 1373 Size: 32 2626 Fragments arranged by size: 2626 2541 1403 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4251 4497 669 Size: 988 281 2313 246 3079 Fragments arranged by size: 3079 2313 988 281 246 HindIII A'AGCT_T Cuts at: 748 2403 748 Size: 1655 5252 HpaI GTT'AAC Cuts at: 4497 4497 Size: 6907 KpnI G_GTAC'C Cuts at: 4232 4232 Size: 6907 MfeI C'AATT_G Cuts at: 4506 4506 Size: 6907 MluI A'CGCG_T Cuts at: 6783 6783 Size: 6907 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2127 2471 2928 3873 6021 6205 1305 Size: 822 344 457 945 2148 184 2007 Fragments arranged by size: 2148 2007 945 822 457 344 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3218 10 Size: 54 1425 343 1386 3699 Fragments arranged by size: 3699 1425 1386 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4865 5224 5383 518 Size: 648 3699 359 159 2042 Fragments arranged by size: 3699 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2051 2137 2669 3308 3430 3451 3670 4050 Size: 445 86 532 639 122 21 219 380 Cuts at: 4050 4057 4170 4323 5000 6148 6393 1606 Size: 7 113 153 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 639 532 445 380 245 219 153 122 113 86 21 7 NaeI GCC'GGC Cuts at: 2499 3661 2499 Size: 1162 5745 NcoI C'CATG_G Cuts at: 513 1065 3219 3315 513 Size: 552 2154 96 4105 Fragments arranged by size: 4105 2154 552 96 NdeI CA'TA_TG Cuts at: 387 387 Size: 6907 NgoMIV G'CCGG_C Cuts at: 2497 3659 2497 Size: 1162 5745 NheI G'CTAG_C Cuts at: 1052 4000 1052 Size: 2948 3959 NotI GC'GGCC_GC Cuts at: 4354 4354 Size: 6907 NspI r_CATG'y Cuts at: 1132 1229 2149 4265 5810 1132 Size: 97 920 2116 1545 2229 Fragments arranged by size: 2229 2116 1545 920 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6907 PciI A'CATG_T Cuts at: 5806 5806 Size: 6907 PflMI CCAn_nnn'nTGG Cuts at: 1108 2209 1108 Size: 1101 5806 PfoI T'CCnGG_A Cuts at: 3234 3234 Size: 6907 PmeI GTTT'AAAC Cuts at: 4211 4211 Size: 6907 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4976 5008 6489 6521 4976 Size: 32 1481 32 5362 Fragments arranged by size: 5362 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2896 2896 Size: 6907 PshAI GACnn'nnGTC Cuts at: 1613 2603 1613 Size: 990 5917 PsiI TTA'TAA Cuts at: 2367 4477 2367 Size: 2110 4797 PspOMI G'GGCC_C Cuts at: 2071 4114 2071 Size: 2043 4864 PstI C_TGCA'G Cuts at: 830 2142 3423 4041 4259 830 Size: 1312 1281 618 218 3478 Fragments arranged by size: 3478 1312 1281 618 218 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6907 PvuII CAG'CTG Cuts at: 2137 2669 3430 3451 3670 4057 2137 Size: 532 761 21 219 387 4987 Fragments arranged by size: 4987 761 532 387 219 21 SacI G_AGCT'C Cuts at: 3362 3362 Size: 6907 SacII CC_GC'GG Cuts at: 2052 3309 2052 Size: 1257 5650 SalI G'TCGA_C Cuts at: 1936 4249 1936 Size: 2313 4594 SapI GCTCTTCn'nnn_ Cuts at: 3367 3367 Size: 6907 SbfI CC_TGCA'GG Cuts at: 4259 4259 Size: 6907 ScaI AGT'ACT Cuts at: 5155 6867 5155 Size: 1712 5195 SfcI C'TryA_G Cuts at: 826 1045 2138 3419 4037 4255 5390 6071 6262 Size: 219 1093 1281 618 218 1135 681 191 Cuts at: 6262 826 Size: 1471 Fragments arranged by size: 1471 1281 1135 1093 681 618 219 218 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6907 SgrAI Cr'CCGG_yG Cuts at: 1700 3289 1700 Size: 1589 5318 SmaI CCC'GGG Cuts at: 1872 2070 3787 4234 1872 Size: 198 1717 447 4545 Fragments arranged by size: 4545 1717 447 198 SmlI C'TyrA_G Cuts at: 820 1017 1943 5011 5912 6174 820 Size: 197 926 3068 901 262 1553 Fragments arranged by size: 3068 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4204 4698 493 Size: 3711 494 2702 Fragments arranged by size: 3711 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6907 SphI G_CATG'C Cuts at: 1132 4265 1132 Size: 3133 3774 SspI AAT'ATT Cuts at: 5 52 4831 5 Size: 47 4779 2081 Fragments arranged by size: 4779 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6907 StyI C'CwwG_G Cuts at: 513 1065 3219 3315 3585 4346 513 Size: 552 2154 96 270 761 3074 Fragments arranged by size: 3074 2154 761 552 270 96 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2641 2710 4934 4951 5104 5289 5734 Size: 1255 1061 69 2224 17 153 185 445 Cuts at: 5734 325 Size: 1498 Fragments arranged by size: 2224 1498 1255 1061 445 185 153 69 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2142 5153 6865 Size: 274 80 33 51 1443 165 3011 1712 Cuts at: 6865 96 Size: 138 Fragments arranged by size: 3011 1712 1443 274 165 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2685 2717 2755 2787 6867 6899 2685 Size: 32 38 32 4080 32 2693 Fragments arranged by size: 4080 2693 38 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6907 XbaI T'CTAG_A Cuts at: 4243 4243 Size: 6907 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2704 2896 2704 Size: 192 6715 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6907 XmaI C'CCGG_G Cuts at: 1870 2068 3785 4232 1870 Size: 198 1717 447 4545 Fragments arranged by size: 4545 1717 447 198 XmnI GAAnn'nnTTC Cuts at: 3953 5036 3953 Size: 1083 5824 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2781 4715 276 Size: 53 83 186 936 1247 1934 2468 Fragments arranged by size: 2468 1934 1247 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BbeI BfrBI BsiWI BstZ17I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SanDI SexAI SfiI SfoI SrfI SwaI