(Circular) (Six-base) MAPSORT of: pFN21ASDA0039.seq Check: 677 from: 1 to: 6127 pFN21ASDA0039 6127 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2569 2569 Size: 6127 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3937 278 Size: 53 83 186 936 2401 2468 Fragments arranged by size: 2468 2401 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3234 3470 1937 Size: 1297 236 4594 Fragments arranged by size: 4594 1297 236 Acc65I G'GTAC_C Cuts at: 3448 3448 Size: 6127 AclI AA'CG_TT Cuts at: 1563 4254 4627 1563 Size: 2691 373 3063 Fragments arranged by size: 3063 2691 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4190 5574 5842 1632 Size: 2558 1384 268 1917 Fragments arranged by size: 2558 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5930 AflIII A'CryG_T Cuts at: 1203 2505 3175 5026 6003 1203 Size: 1302 670 1851 977 1327 Fragments arranged by size: 1851 1327 1302 977 670 AgeI A'CCGG_T Cuts at: 4958 4958 Size: 6127 AhdI GACnn_n'nnGTC Cuts at: 4856 4856 Size: 6127 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6127 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6127 AlwNI CAG_nnn'CTG Cuts at: 2143 2828 2891 3870 5442 2143 Size: 685 63 979 1572 2828 Fragments arranged by size: 2828 1572 979 685 63 ApaI G_GGCC'C Cuts at: 3350 3350 Size: 6127 ApaLI G'TGCA_C Cuts at: 4184 5340 4184 Size: 1156 4971 ApoI r'AATT_y Cuts at: 1493 2176 2414 2759 3436 3662 3814 5923 1493 Size: 683 238 345 677 226 152 2109 1697 Fragments arranged by size: 2109 1697 683 677 345 238 226 152 AseI AT'TA_AT Cuts at: 160 1032 4681 160 Size: 872 3649 1606 Fragments arranged by size: 3649 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6127 AvaI C'yCGr_G Cuts at: 1870 1943 3452 1870 Size: 73 1509 4545 Fragments arranged by size: 4545 1509 73 BamHI G'GATC_C Cuts at: 3457 3824 3457 Size: 367 5760 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2704 3448 4903 618 Size: 325 217 627 917 744 1455 1842 Fragments arranged by size: 1842 1455 917 744 627 325 217 BanII G_rGCy'C Cuts at: 1391 2188 2455 3146 3350 3446 1391 Size: 797 267 691 204 96 4072 Fragments arranged by size: 4072 797 691 267 204 96 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2466 3287 928 Size: 971 567 821 3768 Fragments arranged by size: 3768 971 821 567 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4318 4352 1903 Size: 34 2381 34 3678 Fragments arranged by size: 3678 2381 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4019 5229 4019 Size: 1210 4917 BclI T'GATC_A Cuts at: 1548 1548 Size: 6127 BfrBI ATG'CAT Cuts at: 2684 2684 Size: 6127 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4738 136 Size: 107 122 71 631 605 3066 1525 Fragments arranged by size: 3066 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6122 6122 Size: 6127 BlpI GC'TnA_GC Cuts at: 2099 3544 2099 Size: 1445 4682 Bme1580I G_kGCm'C Cuts at: 1790 3350 4188 5344 1790 Size: 1560 838 1156 2573 Fragments arranged by size: 2573 1560 1156 838 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6127 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3858 4816 442 Size: 416 53 83 399 24 2441 958 1753 Fragments arranged by size: 2441 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2300 1056 Size: 1244 4883 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4787 1239 Size: 3548 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3130 4252 5117 5415 3130 Size: 1122 865 298 3842 Fragments arranged by size: 3842 1122 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4790 882 Size: 617 3291 2219 Fragments arranged by size: 3291 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3424 3918 493 Size: 711 2220 494 2702 Fragments arranged by size: 2702 2220 711 494 BsaBI GATnn'nnATC Cuts at: 6121 6121 Size: 6127 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3934 4316 4951 275 Size: 53 83 186 936 2401 382 635 1451 Fragments arranged by size: 2401 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4560 4958 5232 5379 5790 4560 Size: 398 274 147 411 4897 Fragments arranged by size: 4897 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5902 Fragments arranged by size: 5902 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2998 3069 3072 3281 1186 Size: 1812 71 3 209 4032 Fragments arranged by size: 4032 1812 209 71 3 BseYI C'CCAG_C Cuts at: 2580 5330 5826 2580 Size: 2750 496 2881 Fragments arranged by size: 2881 2750 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2454 3235 1163 Size: 573 718 781 4055 Fragments arranged by size: 4055 781 718 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3577 4338 5366 1540 Size: 275 186 1576 761 1028 2301 Fragments arranged by size: 2301 1576 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2455 3146 4188 4273 5344 2455 Size: 691 1042 85 1071 3238 Fragments arranged by size: 3238 1071 1042 691 85 BsmI GAATG_Cn' Cuts at: 3638 3731 3638 Size: 93 6034 BsmBI CGTCTCn'nnnn_ Cuts at: 3093 3093 Size: 6127 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2188 2455 3146 3350 3446 4188 4273 Size: 399 398 267 691 204 96 742 85 Cuts at: 4273 5344 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 691 399 398 267 204 96 85 BspEI T'CCGG_A Cuts at: 5790 5790 Size: 6127 BspHI T'CATG_A Cuts at: 4016 5746 4016 Size: 1730 4397 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2563 2569 3482 844 Size: 1719 6 913 3489 Fragments arranged by size: 3489 1719 913 6 BsrBI CCG'CTC Cuts at: 1394 1823 4014 1394 Size: 429 2191 3507 Fragments arranged by size: 3507 2191 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4622 4796 65 Size: 1183 3374 174 1396 Fragments arranged by size: 3374 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4771 4958 1700 Size: 110 2961 187 2869 Fragments arranged by size: 2961 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2322 96 Size: 2226 3901 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6127 BssSI C'ACGA_G Cuts at: 4187 5199 4187 Size: 1012 5115 BstAPI GCAn_nnn'nTGC Cuts at: 794 2855 794 Size: 2061 4066 BstBI TT'CG_AA Cuts at: 2007 4931 2007 Size: 2924 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6127 BstYI r'GATC_y Cuts at: 1972 3457 3824 4209 4226 5678 5764 5776 6122 Size: 1485 367 385 17 1452 86 12 346 Cuts at: 6122 1972 Size: 1977 Fragments arranged by size: 1977 1485 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 513 Size: 552 957 4618 Fragments arranged by size: 4618 957 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5388 Fragments arranged by size: 5388 683 56 BtsI GCAGTG_nn' Cuts at: 2289 2796 3022 3643 4437 4457 2289 Size: 507 226 621 794 20 3959 Fragments arranged by size: 3959 794 621 507 226 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6127 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6092 DraI TTT'AAA Cuts at: 2764 3431 2764 Size: 667 5460 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2060 5134 809 Size: 507 744 3074 1802 Fragments arranged by size: 3074 1802 744 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3574 4463 6092 Size: 54 1425 50 275 18 1744 889 1629 Cuts at: 6092 8 Size: 43 Fragments arranged by size: 1744 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3574 1537 Size: 275 1762 4090 Fragments arranged by size: 4090 1762 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2473 3258 4057 1353 Size: 1120 785 799 3423 Fragments arranged by size: 3423 1120 799 785 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2042 4714 5088 5234 387 Size: 1064 138 453 2672 374 146 1280 Fragments arranged by size: 2672 1280 1064 453 374 146 138 EcoICRI GAG'CTC Cuts at: 2453 3144 2453 Size: 691 5436 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 4190 4787 5574 5842 1239 Size: 393 2558 597 787 268 1524 Fragments arranged by size: 2558 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 3346 3347 3346 Size: 1 6126 EcoRI G'AATT_C Cuts at: 3436 3814 3436 Size: 378 5749 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3287 3287 Size: 6127 FspI TGC'GCA Cuts at: 4633 4633 Size: 6127 HaeII r_GCGC'y Cuts at: 1678 5274 1678 Size: 3596 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3053 3085 3230 3262 3352 Size: 32 135 32 1481 32 145 32 90 Cuts at: 3352 3384 4768 4800 4842 4874 1373 Size: 32 1384 32 42 32 2626 Fragments arranged by size: 2626 1481 1384 145 135 90 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2125 3471 3717 669 Size: 988 281 187 1346 246 3079 Fragments arranged by size: 3079 1346 988 281 246 187 HindIII A'AGCT_T Cuts at: 748 748 Size: 6127 HpaI GTT'AAC Cuts at: 2125 3717 2125 Size: 1592 4535 KpnI G_GTAC'C Cuts at: 3452 3452 Size: 6127 MfeI C'AATT_G Cuts at: 3726 3726 Size: 6127 MluI A'CGCG_T Cuts at: 3175 6003 3175 Size: 2828 3299 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5241 5425 1305 Size: 3936 184 2007 Fragments arranged by size: 3936 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4305 Fragments arranged by size: 4305 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2092 2318 3314 4085 4444 4603 518 Size: 648 926 226 996 771 359 159 2042 Fragments arranged by size: 2042 996 926 771 648 359 226 159 MspA1I CmG'CkG Cuts at: 1606 2438 3543 4220 5368 5613 1606 Size: 832 1105 677 1148 245 2120 Fragments arranged by size: 2120 1148 1105 832 677 245 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 4618 Fragments arranged by size: 4618 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6127 NheI G'CTAG_C Cuts at: 1052 2296 1052 Size: 1244 4883 NotI GC'GGCC_GC Cuts at: 3574 3574 Size: 6127 NsiI A_TGCA'T Cuts at: 2686 2686 Size: 6127 NspI r_CATG'y Cuts at: 1132 1229 2198 2509 3485 5030 1132 Size: 97 969 311 976 1545 2229 Fragments arranged by size: 2229 1545 976 969 311 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6127 PciI A'CATG_T Cuts at: 2505 5026 2505 Size: 2521 3606 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6127 PmeI GTTT'AAAC Cuts at: 3431 3431 Size: 6127 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4196 4228 5709 5741 4196 Size: 32 1481 32 4582 Fragments arranged by size: 4582 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3391 1613 Size: 1778 4349 PsiI TTA'TAA Cuts at: 3697 3697 Size: 6127 PspOMI G'GGCC_C Cuts at: 3346 3346 Size: 6127 PstI C_TGCA'G Cuts at: 830 2411 2692 2860 2907 3479 830 Size: 1581 281 168 47 572 3478 Fragments arranged by size: 3478 1581 572 281 168 47 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6127 PvuII CAG'CTG Cuts at: 2438 2438 Size: 6127 SacI G_AGCT'C Cuts at: 2455 3146 2455 Size: 691 5436 SalI G'TCGA_C Cuts at: 1936 3469 1936 Size: 1533 4594 SbfI CC_TGCA'GG Cuts at: 2860 3479 2860 Size: 619 5508 ScaI AGT'ACT Cuts at: 4375 6087 4375 Size: 1712 4415 SfcI C'TryA_G Cuts at: 826 1045 2407 2688 2820 2856 2903 3475 4610 Size: 219 1362 281 132 36 47 572 1135 Cuts at: 4610 5291 5482 826 Size: 681 191 1471 Fragments arranged by size: 1471 1362 1135 681 572 281 219 191 132 47 36 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6127 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6127 SmaI CCC'GGG Cuts at: 1872 3454 1872 Size: 1582 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3145 4231 5132 5394 820 Size: 197 926 1202 1086 901 262 1553 Fragments arranged by size: 1553 1202 1086 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3424 3918 493 Size: 2931 494 2702 Fragments arranged by size: 2931 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6127 SphI G_CATG'C Cuts at: 1132 3485 1132 Size: 2353 3774 SspI AAT'ATT Cuts at: 5 52 4051 5 Size: 47 3999 2081 Fragments arranged by size: 3999 2081 47 StuI AGG'CCT Cuts at: 1517 2862 1517 Size: 1345 4782 StyI C'CwwG_G Cuts at: 513 1065 2022 3566 513 Size: 552 957 1544 3074 Fragments arranged by size: 3074 1544 957 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4154 4171 4324 4509 4954 325 Size: 1255 2574 17 153 185 445 1498 Fragments arranged by size: 2574 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2322 3292 4373 Size: 274 80 33 51 1443 345 970 1081 Cuts at: 4373 6085 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1081 970 345 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6087 6119 6087 Size: 32 6095 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6127 XbaI T'CTAG_A Cuts at: 3463 3463 Size: 6127 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2596 2596 Size: 6127 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6127 XmaI C'CCGG_G Cuts at: 1870 3452 1870 Size: 1582 4545 XmnI GAAnn'nnTTC Cuts at: 3030 4256 3030 Size: 1226 4901 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3935 276 Size: 53 83 186 936 2401 2468 Fragments arranged by size: 2468 2401 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI