(Circular) (Six-base) MAPSORT of: pFN21ASDA0037.seq Check: 6458 from: 1 to: 7969 pFN21ASDA0037 7969 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2386 3091 4199 2386 Size: 705 1108 6156 Fragments arranged by size: 6156 1108 705 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5779 278 Size: 53 83 186 936 4243 2468 Fragments arranged by size: 4243 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5312 1937 Size: 3375 4594 Acc65I G'GTAC_C Cuts at: 3480 4632 5290 3480 Size: 1152 658 6159 Fragments arranged by size: 6159 1152 658 AclI AA'CG_TT Cuts at: 1563 6096 6469 1563 Size: 4533 373 3063 Fragments arranged by size: 4533 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2167 2431 3470 4427 4802 6032 7416 7684 Size: 535 264 1039 957 375 1230 1384 268 Cuts at: 7684 1632 Size: 1917 Fragments arranged by size: 1917 1384 1230 1039 957 535 375 268 264 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7772 AflIII A'CryG_T Cuts at: 1203 3081 3201 3216 6868 7845 1203 Size: 1878 120 15 3652 977 1327 Fragments arranged by size: 3652 1878 1327 977 120 15 AgeI A'CCGG_T Cuts at: 6800 6800 Size: 7969 AhdI GACnn_n'nnGTC Cuts at: 3205 6698 3205 Size: 3493 4476 AleI CACnn'nnGTG Cuts at: 1166 3119 4905 1166 Size: 1953 1786 4230 Fragments arranged by size: 4230 1953 1786 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7969 AlwNI CAG_nnn'CTG Cuts at: 2563 2904 4018 4882 5251 5712 7284 2563 Size: 341 1114 864 369 461 1572 3248 Fragments arranged by size: 3248 1572 1114 864 461 369 341 ApaI G_GGCC'C Cuts at: 2069 3802 5035 2069 Size: 1733 1233 5003 Fragments arranged by size: 5003 1733 1233 ApaLI G'TGCA_C Cuts at: 3263 6026 7182 3263 Size: 2763 1156 4050 Fragments arranged by size: 4050 2763 1156 ApoI r'AATT_y Cuts at: 1493 5278 5504 5656 7765 1493 Size: 3785 226 152 2109 1697 Fragments arranged by size: 3785 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6523 160 Size: 872 5491 1606 Fragments arranged by size: 5491 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7969 AvaI C'yCGr_G Cuts at: 1870 1943 2637 3151 3485 3795 3955 3972 5153 Size: 73 694 514 334 310 160 17 1181 Cuts at: 5153 5294 1870 Size: 141 4545 Fragments arranged by size: 4545 1181 694 514 334 310 160 141 73 17 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 5149 5182 5149 Size: 33 7936 BamHI G'GATC_C Cuts at: 5299 5666 5299 Size: 367 7602 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2398 2603 2844 3480 3784 Size: 325 217 627 611 205 241 636 304 Cuts at: 3784 4367 4379 4632 5290 6745 618 Size: 583 12 253 658 1455 1842 Fragments arranged by size: 1842 1455 658 636 627 611 583 325 304 253 241 217 205 12 BanII G_rGCy'C Cuts at: 1391 2069 2943 3690 3802 4165 4176 4776 4851 Size: 678 874 747 112 363 11 600 75 Cuts at: 4851 5035 5288 1391 Size: 184 253 4072 Fragments arranged by size: 4072 874 747 678 600 363 253 184 112 75 11 BbeI G_GCGC'C Cuts at: 2607 2607 Size: 7969 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2413 4219 4415 928 Size: 971 514 1806 196 4482 Fragments arranged by size: 4482 1806 971 514 196 BbvCI CC'TCA_GC Cuts at: 4022 5207 4022 Size: 1185 6784 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3829 3863 6160 6194 1903 Size: 34 1892 34 2297 34 3678 Fragments arranged by size: 3678 2297 1892 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5861 7071 5861 Size: 1210 6759 BclI T'GATC_A Cuts at: 1548 5181 1548 Size: 3633 4336 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2162 2474 4280 Size: 107 122 71 631 605 490 312 1806 Cuts at: 4280 4592 6580 136 Size: 312 1988 1525 Fragments arranged by size: 1988 1806 1525 631 605 490 312 312 122 107 71 BglII A'GATC_T Cuts at: 7964 7964 Size: 7969 BlpI GC'TnA_GC Cuts at: 3689 4850 5386 3689 Size: 1161 536 6272 Fragments arranged by size: 6272 1161 536 Bme1580I G_kGCm'C Cuts at: 1790 2069 2403 3267 3324 3802 4869 5035 6030 Size: 279 334 864 57 478 1067 166 995 Cuts at: 6030 7186 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 1067 995 864 478 334 279 166 57 BmgBI CAC'GTC Cuts at: 1318 3202 3217 3559 1318 Size: 1884 15 342 5728 Fragments arranged by size: 5728 1884 342 15 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2532 3893 3926 Size: 416 53 83 399 24 1115 1361 33 Cuts at: 3926 5700 6658 442 Size: 1774 958 1753 Fragments arranged by size: 1774 1753 1361 1115 958 416 399 83 53 33 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7969 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3667 3667 Size: 7969 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2185 3773 4265 4447 4589 4748 4904 5040 Size: 946 1588 492 182 142 159 156 136 Cuts at: 5040 5118 5129 6629 1239 Size: 78 11 1500 2579 Fragments arranged by size: 2579 1588 1500 946 492 182 159 156 142 136 78 11 Bpu10I CC'TnA_GC Cuts at: 2300 4022 5207 2300 Size: 1722 1185 5062 Fragments arranged by size: 5062 1722 1185 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2765 3332 6094 6959 7257 2765 Size: 567 2762 865 298 3477 Fragments arranged by size: 3477 2762 865 567 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3545 4532 5123 6632 882 Size: 617 2046 987 591 1509 2219 Fragments arranged by size: 2219 2046 1509 987 617 591 BsaAI yAC'GTr Cuts at: 493 1204 4591 4830 5266 5760 493 Size: 711 3387 239 436 494 2702 Fragments arranged by size: 3387 2702 711 494 436 239 BsaBI GATnn'nnATC Cuts at: 3359 7963 3359 Size: 4604 3365 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2604 3461 5776 6158 Size: 53 83 186 936 1071 857 2315 382 Cuts at: 6158 6793 275 Size: 635 1451 Fragments arranged by size: 2315 1451 1071 936 857 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3231 6402 6800 7074 7221 7632 3231 Size: 3171 398 274 147 411 3568 Fragments arranged by size: 3568 3171 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4949 4979 1178 Size: 30 165 30 3546 30 4168 Fragments arranged by size: 4168 3546 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2075 4544 5141 1186 Size: 889 2469 597 4014 Fragments arranged by size: 4014 2469 889 597 BseYI C'CCAG_C Cuts at: 2341 3410 3591 3928 3967 5251 7172 7668 2341 Size: 1069 181 337 39 1284 1921 496 2642 Fragments arranged by size: 2642 1921 1284 1069 496 337 181 39 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3970 5119 1163 Size: 573 2234 1149 4013 Fragments arranged by size: 4013 2234 1149 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3259 3641 5419 6180 7208 1540 Size: 275 186 1258 382 1778 761 1028 2301 Fragments arranged by size: 2301 1778 1258 1028 761 382 275 186 BsiHKAI G_wGCw'C Cuts at: 2508 2943 3267 4296 4527 4776 6030 6115 7186 Size: 435 324 1029 231 249 1254 85 1071 Cuts at: 7186 2508 Size: 3291 Fragments arranged by size: 3291 1254 1071 1029 435 324 249 231 85 BsiWI C'GTAC_G Cuts at: 3303 3654 3303 Size: 351 7618 BsmI GAATG_Cn' Cuts at: 5174 5480 5573 5174 Size: 306 93 7570 Fragments arranged by size: 7570 306 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2485 2485 Size: 7969 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2069 2403 2508 2943 3267 3324 3690 Size: 399 279 334 105 435 324 57 366 Cuts at: 3690 3802 4165 4176 4296 4527 4776 4851 4869 Size: 112 363 11 120 231 249 75 18 Cuts at: 4869 5035 5288 6030 6115 7186 1391 Size: 166 253 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 435 399 366 363 334 324 279 253 249 231 166 120 112 105 85 75 57 18 11 BspEI T'CCGG_A Cuts at: 7632 7632 Size: 7969 BspHI T'CATG_A Cuts at: 5858 7588 5858 Size: 1730 6239 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2386 2724 3091 4089 4199 5141 5324 844 Size: 1542 338 367 998 110 942 183 3489 Fragments arranged by size: 3489 1542 998 942 367 338 183 110 BsrBI CCG'CTC Cuts at: 1394 1823 4888 5856 1394 Size: 429 3065 968 3507 Fragments arranged by size: 3507 3065 968 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2175 4129 4755 6464 6638 65 Size: 1183 927 1954 626 1709 174 1396 Fragments arranged by size: 1954 1709 1396 1183 927 626 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6613 6800 1700 Size: 110 4803 187 2869 Fragments arranged by size: 4803 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2430 96 Size: 2334 5635 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7969 BssSI C'ACGA_G Cuts at: 3697 4526 4868 6029 7041 3697 Size: 829 342 1161 1012 4625 Fragments arranged by size: 4625 1161 1012 829 342 BstAPI GCAn_nnn'nTGC Cuts at: 794 2623 794 Size: 1829 6140 BstBI TT'CG_AA Cuts at: 2007 6773 2007 Size: 4766 3203 BstEII G'GTnAC_C Cuts at: 1184 2792 5119 1184 Size: 1608 2327 4034 Fragments arranged by size: 4034 2327 1608 BstXI CCAn_nnnn'nTGG Cuts at: 2920 3121 3235 3751 5086 2920 Size: 201 114 516 1335 5803 Fragments arranged by size: 5803 1335 516 201 114 BstYI r'GATC_y Cuts at: 1972 2673 2994 5299 5666 6051 6068 7520 7606 Size: 701 321 2305 367 385 17 1452 86 Cuts at: 7606 7618 7964 1972 Size: 12 346 1977 Fragments arranged by size: 2305 1977 1452 701 385 367 346 321 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4232 4861 4232 Size: 629 7340 BtgI C'CryG_G Cuts at: 513 1065 2748 3114 4542 5002 5173 513 Size: 552 1683 366 1428 460 171 3309 Fragments arranged by size: 3309 1683 1428 552 460 366 171 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4198 4722 4912 487 Size: 683 56 2972 524 190 3544 Fragments arranged by size: 3544 2972 683 524 190 56 BtsI GCAGTG_nn' Cuts at: 2151 2484 3550 4325 5232 5485 6279 6299 2151 Size: 333 1066 775 907 253 794 20 3821 Fragments arranged by size: 3821 1066 907 794 775 333 253 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7969 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7934 DraI TTT'AAA Cuts at: 5273 5273 Size: 7969 DraIII CAC_nnn'GTG Cuts at: 2540 3217 3887 4704 2540 Size: 677 670 817 5805 Fragments arranged by size: 5805 817 677 670 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6976 809 Size: 507 5660 1802 Fragments arranged by size: 5660 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2145 2565 2763 Size: 54 1425 50 275 18 315 420 198 Cuts at: 2763 2895 3225 3256 4035 4068 4140 4333 5076 Size: 132 330 31 779 33 72 193 743 Cuts at: 5076 5416 6305 7934 8 Size: 340 889 1629 43 Fragments arranged by size: 1629 1425 889 779 743 420 340 330 315 275 198 193 132 72 54 50 43 33 31 18 EagI C'GGCC_G Cuts at: 1537 1812 3256 5416 1537 Size: 275 1444 2160 4090 Fragments arranged by size: 4090 2160 1444 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2651 4301 5899 1353 Size: 1298 1650 1598 3423 Fragments arranged by size: 3423 1650 1598 1298 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2666 3586 4970 6556 6930 7076 Size: 1064 138 1077 920 1384 1586 374 146 Cuts at: 7076 387 Size: 1280 Fragments arranged by size: 1586 1384 1280 1077 1064 920 374 146 138 EcoICRI GAG'CTC Cuts at: 2941 4774 2941 Size: 1833 6136 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2167 2185 2431 3470 3773 4265 4427 Size: 393 535 18 246 1039 303 492 162 Cuts at: 4427 4447 4589 4748 4802 4904 5040 5118 5129 Size: 20 142 159 54 102 136 78 11 Cuts at: 5129 6032 6629 7416 7684 1239 Size: 903 597 787 268 1524 Fragments arranged by size: 1524 1039 903 787 597 535 492 393 303 268 246 162 159 142 136 102 78 54 20 18 11 EcoNI CCTnn'n_nnAGG Cuts at: 2051 3296 3755 2051 Size: 1245 459 6265 Fragments arranged by size: 6265 1245 459 EcoO109I rG'GnC_Cy Cuts at: 2065 2066 2194 2304 2641 3337 3702 4402 5031 Size: 1 128 110 337 696 365 700 629 Cuts at: 5031 5149 2065 Size: 118 4885 Fragments arranged by size: 4885 700 696 629 365 337 128 118 110 1 EcoRI G'AATT_C Cuts at: 5278 5656 5278 Size: 378 7591 FspI TGC'GCA Cuts at: 2690 3185 3470 6475 2690 Size: 495 285 3005 4184 Fragments arranged by size: 4184 3005 495 285 FspAI rTGC'GCAy Cuts at: 3470 3470 Size: 7969 HaeII r_GCGC'y Cuts at: 1678 2041 2296 2315 2342 2463 2607 2704 3598 Size: 363 255 19 27 121 144 97 894 Cuts at: 3598 7116 1678 Size: 3518 2531 Fragments arranged by size: 3518 2531 894 363 255 144 121 97 27 19 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3191 3223 3656 3688 6610 Size: 32 135 32 1619 32 433 32 2922 Cuts at: 6610 6642 6684 6716 1373 Size: 32 42 32 2626 Fragments arranged by size: 2922 2626 1619 433 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4714 5313 5559 669 Size: 988 281 2776 599 246 3079 Fragments arranged by size: 3079 2776 988 599 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7969 HpaI GTT'AAC Cuts at: 5559 5559 Size: 7969 KasI G'GCGC_C Cuts at: 2603 2603 Size: 7969 KpnI G_GTAC'C Cuts at: 3484 4636 5294 3484 Size: 1152 658 6159 Fragments arranged by size: 6159 1152 658 MfeI C'AATT_G Cuts at: 5568 5568 Size: 7969 MluI A'CGCG_T Cuts at: 7845 7845 Size: 7969 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3621 7083 7267 1305 Size: 2316 3462 184 2007 Fragments arranged by size: 3462 2316 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2567 2765 2897 3227 4070 Size: 54 1425 343 735 198 132 330 843 Cuts at: 4070 5078 10 Size: 1008 2901 Fragments arranged by size: 2901 1425 1008 843 735 343 330 198 132 54 MslI CAynn'nnrTG Cuts at: 518 1166 2747 2753 2850 3119 3131 3137 4832 Size: 648 1581 6 97 269 12 6 1695 Cuts at: 4832 4905 5927 6286 6445 518 Size: 73 1022 359 159 2042 Fragments arranged by size: 2042 1695 1581 1022 648 359 269 159 97 73 12 6 6 MspA1I CmG'CkG Cuts at: 1606 2122 2560 2893 3334 4197 4797 4841 5385 Size: 516 438 333 441 863 600 44 544 Cuts at: 5385 6062 7210 7455 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 863 677 600 544 516 441 438 333 245 44 NarI GG'CG_CC Cuts at: 2604 2604 Size: 7969 NcoI C'CATG_G Cuts at: 513 1065 2748 4542 5002 513 Size: 552 1683 1794 460 3480 Fragments arranged by size: 3480 1794 1683 552 460 NdeI CA'TA_TG Cuts at: 387 387 Size: 7969 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7969 NotI GC'GGCC_GC Cuts at: 5416 5416 Size: 7969 NspI r_CATG'y Cuts at: 1132 1229 2455 2809 2986 3085 3136 3625 4898 Size: 97 1226 354 177 99 51 489 1273 Cuts at: 4898 5327 6872 1132 Size: 429 1545 2229 Fragments arranged by size: 2229 1545 1273 1226 489 429 354 177 99 97 51 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7969 PciI A'CATG_T Cuts at: 3081 6868 3081 Size: 3787 4182 PflMI CCAn_nnn'nTGG Cuts at: 1108 2500 4342 4882 1108 Size: 1392 1842 540 4195 Fragments arranged by size: 4195 1842 1392 540 PfoI T'CCnGG_A Cuts at: 3750 4394 3750 Size: 644 7325 PmeI GTTT'AAAC Cuts at: 5273 5273 Size: 7969 PmlI CAC'GTG Cuts at: 4591 4591 Size: 7969 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6038 6070 7551 7583 6038 Size: 32 1481 32 6424 Fragments arranged by size: 6424 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2194 2641 3337 4402 2194 Size: 447 696 1065 5761 Fragments arranged by size: 5761 1065 696 447 PshAI GACnn'nnGTC Cuts at: 1613 3557 1613 Size: 1944 6025 PsiI TTA'TAA Cuts at: 5539 5539 Size: 7969 PspOMI G'GGCC_C Cuts at: 2065 3798 5031 2065 Size: 1733 1233 5003 Fragments arranged by size: 5003 1733 1233 PspXI vC'TCGA_Gb Cuts at: 3485 3485 Size: 7969 PstI C_TGCA'G Cuts at: 830 2559 4196 4846 5321 830 Size: 1729 1637 650 475 3478 Fragments arranged by size: 3478 1729 1637 650 475 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7969 PvuII CAG'CTG Cuts at: 2560 2893 4197 4841 2560 Size: 333 1304 644 5688 Fragments arranged by size: 5688 1304 644 333 SacI G_AGCT'C Cuts at: 2943 4776 2943 Size: 1833 6136 SalI G'TCGA_C Cuts at: 1936 5311 1936 Size: 3375 4594 SanDI GG'GwC_CC Cuts at: 2194 3337 2194 Size: 1143 6826 SapI GCTCTTCn'nnn_ Cuts at: 4301 4301 Size: 7969 SbfI CC_TGCA'GG Cuts at: 5321 5321 Size: 7969 ScaI AGT'ACT Cuts at: 6217 7929 6217 Size: 1712 6257 SexAI A'CCwGG_T Cuts at: 3372 4287 4428 3372 Size: 915 141 6913 Fragments arranged by size: 6913 915 141 SfcI C'TryA_G Cuts at: 826 1045 2555 4192 4842 5317 6452 7133 7324 Size: 219 1510 1637 650 475 1135 681 191 Cuts at: 7324 826 Size: 1471 Fragments arranged by size: 1637 1510 1471 1135 681 650 475 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 4280 4280 Size: 7969 SfoI GGC'GCC Cuts at: 2605 2605 Size: 7969 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7969 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7969 SmaI CCC'GGG Cuts at: 1872 2639 3797 5296 1872 Size: 767 1158 1499 4545 Fragments arranged by size: 4545 1499 1158 767 SmlI C'TyrA_G Cuts at: 820 1017 1943 2780 3347 3485 3955 6073 6974 Size: 197 926 837 567 138 470 2118 901 Cuts at: 6974 7236 820 Size: 262 1553 Fragments arranged by size: 2118 1553 926 901 837 567 470 262 197 138 SnaBI TAC'GTA Cuts at: 493 5266 5760 493 Size: 4773 494 2702 Fragments arranged by size: 4773 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7969 SphI G_CATG'C Cuts at: 1132 2455 2809 2986 4898 5327 1132 Size: 1323 354 177 1912 429 3774 Fragments arranged by size: 3774 1912 1323 429 354 177 SspI AAT'ATT Cuts at: 5 52 5893 5 Size: 47 5841 2081 Fragments arranged by size: 5841 2081 47 StuI AGG'CCT Cuts at: 1517 3577 4134 4341 1517 Size: 2060 557 207 5145 Fragments arranged by size: 5145 2060 557 207 StyI C'CwwG_G Cuts at: 513 1065 2031 2307 2748 2913 2967 3432 3630 Size: 552 966 276 441 165 54 465 198 Cuts at: 3630 4336 4542 5002 5408 513 Size: 706 206 460 406 3074 Fragments arranged by size: 3074 966 706 552 465 460 441 406 276 206 198 165 54 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2551 3802 3875 4213 5996 6013 6166 Size: 1255 971 1251 73 338 1783 17 153 Cuts at: 6166 6351 6796 325 Size: 185 445 1498 Fragments arranged by size: 1783 1498 1255 1251 971 445 338 185 153 73 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2430 3913 6215 Size: 274 80 33 51 1443 453 1483 2302 Cuts at: 6215 7927 96 Size: 1712 138 Fragments arranged by size: 2302 1712 1483 1443 453 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2526 2558 3203 3235 7929 7961 2526 Size: 32 645 32 4694 32 2534 Fragments arranged by size: 4694 2534 645 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4652 1316 Size: 3336 4633 XbaI T'CTAG_A Cuts at: 5305 5305 Size: 7969 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2158 2221 2545 2920 4317 4426 2158 Size: 63 324 375 1397 109 5701 Fragments arranged by size: 5701 1397 375 324 109 63 XhoI C'TCGA_G Cuts at: 1943 3485 3955 1943 Size: 1542 470 5957 Fragments arranged by size: 5957 1542 470 XmaI C'CCGG_G Cuts at: 1870 2637 3795 5294 1870 Size: 767 1158 1499 4545 Fragments arranged by size: 4545 1499 1158 767 XmnI GAAnn'nnTTC Cuts at: 4511 6098 4511 Size: 1587 6382 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5777 276 Size: 53 83 186 936 4243 2468 Fragments arranged by size: 4243 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BfrBI BstZ17I EcoRV FalI FseI NaeI NgoMIV NruI NsiI PacI PsrI RsrII SacII SrfI SwaI