Restriction map for
pFN21ASDA0032
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# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BanI BbeI
BbvCI BcgI BclI BfrI BlnI
BsaHI BsaWI BseAI BsiWI BsmBI
Bsp1407I BspDI BspEI BsrBI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstUI BstZ17I Bsu36I ClaI CpoI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV FbaI
FseI FspI HgaI Hin1I HindIII
KasI KpnI KspI MfeI MluI
MroI MunI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmeI PshAI PshBI Psp1406I RsrII
SacII SalI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI SspBI
StuI SwaI Tth111I XhoI
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# ONE CUT ENZYMES ( 39 enzymes) #
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BamHI 566 GGATCC G^GATCC
BbrPI 2337 CACGTG CAC^GTG
BglI 589 GCCGGAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1097 AGATCT A^GATCT
BsaAI 2337 CACGTG [CT]AC^GT[GA]
BsiEI 2961 CGATCG CG[GA][CT]^CG
BsmI 2159 GCATTC GAATGC(1/-1)
BspLU11I 169 ACATGT A^CATGT
BsrDI 442 CATTGC GCAATG(2/0)
BssSI 1858 CTCGTG CACGAG(-5/-1)
BstAPI 2218 GCAGTGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CfoI 800 GCGC GCG^C
DraIII 133 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 97 GACAGAAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 495 CCATGG C^C[GA][CT]GG
EcoNI 1542 CCTACTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 950 ATGCAT ATGCA^T
FauI 3020 GCGGG CCCGC(4/6)
HaeII 799 GGCGCT [GA]GCGC^[CT]
HhaI 800 GCGC GCG^C
HinP1I 800 GCGC G^CGC
HpaI 847 GTTAAC GTT^AAC
NcoI 495 CCATGG C^CATGG
NsiI 950 ATGCAT ATGCA^T
PflMI 282 CCACAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2337 CACGTG CAC^GTG
PmlI 2337 CACGTG CAC^GTG
PpuMI 553 AGGTCCC [GA]G^G[AT]CC[CT]
PvuI 2961 CGATCG CGAT^CG
PvuII 310 CAGCTG CAG^CTG
SapI 2769 GCTCTTC GCTCTTC(1/4)
SbfI 51 CCTGCAGG CCTGCA^GG
SexAI 33 ACCTGGT A^CC[AT]GGT
SmaI 2533 CCCGGG CCC^GGG
SmlI 2764 CTTGAG C^T[CT][GA]AG
Sse8387I 51 CCTGCAGG CCTGCA^GG
Van91I 282 CCACAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 2533 CCCGGG C^CCGGG
XmaI 2533 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AciI 457 GCGG CCGC(-3/-1)
3020 GCGG
AcsI 2245 GAATTT [GA]^AATT[CT]
2662 GAATTT
AlwI 567 GATCC GGATC(4/5)
566 GGATC
AlwNI 623 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
2003 CAGATGCTG
ApoI 2245 GAATTT [GA]^AATT[CT]
2662 GAATTT
AvaI 1061 CCCGAG C^[CT]CG[GA]G
2533 CCCGGG
AvaII 554 GGTCC G^G[AT]CC
2106 GGTCC
BciVI 1608 GGATAC GTATCC(6/5)
1935 GGATAC
BlpI 67 GCTGAGC GC^T[ACGT]AGC
2154 GCTGAGC
Bpu1102I 67 GCTGAGC GC^T[ACGT]AGC
2154 GCTGAGC
BsgI 326 GTGCAG GTGCAG(16/14)
1694 GTGCAG
BsoBI 1061 CCCGAG C^[CT]CG[GA]G
2533 CCCGGG
BsrFI 2227 GCCGGT [GA]^CCGG[CT]
3036 GCCGGT
BstXI 75 CCAGGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1853 CCAATCTCGTGG
BstYI 566 GGATCC [GA]^GATC[CT]
1097 AGATCT
CelII 67 GCTGAGC GC^T[ACGT]AGC
2154 GCTGAGC
Cfr10I 2227 GCCGGT [GA]^CCGG[CT]
3036 GCCGGT
DraI 1075 TTTAAA TTT^AAA
1345 TTTAAA
DraII 553 AGGTCCC [GA]G^G[ACGT]CC[CT]
3116 AGGCCCT
EcoO109I 553 AGGTCCC [GA]G^G[ACGT]CC[CT]
3116 AGGCCCT
HincII 847 GTTAAC GT[CT]^[GA]AC
1162 GTTGAC
HindII 847 GTTAAC GT[CT]^[GA]AC
1162 GTTGAC
MaeII 2114 ACGT A^CGT
2338 ACGT
MflI 566 GGATCC [GA]^GATC[CT]
1097 AGATCT
MslI 258 CATTCATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1249 CACAACAATG
MspA1I 310 CAGCTG C[AC]G^C[GT]G
3018 CAGCGG
SacI 236 GAGCTC GAGCT^C
2767 GAGCTC
SspI 414 AATATT AAT^ATT
1661 AATATT
TaqI 687 TCGA T^CGA
2414 TCGA
Tsp45I 299 GTGAC ^GT[GC]AC
2490 GTCAC
TthHB8I 687 TCGA T^CGA
2414 TCGA
XbaI 864 TCTAGA T^CTAGA
1434 TCTAGA
XhoII 566 GGATCC [GA]^GATC[CT]
1097 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AfaI AflIII AluI Asp700I AspHI
BalI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaBI
BsaI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspHI
BspMI BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII EaeI
EarI EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MamI
MboI MboII MluNI MnlI MscI
MseI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PstI RcaI RsaI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI StyI
TfiI Tru9I TseI Tsp509I TspRI
XcmI XmnI