Restriction map for pFN21ASDA0032
 (Linear) (Six-base) MAPSORT of: KIAA0032.seq  Check: 3855  from: 1  to: 3150



KIAA0032.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:10  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    2013    3150 
   Size:       2013    1137
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1152    2519    2765    3150 
   Size:       1152    1367     246     385

  Fragments arranged by size: 

                1367    1152     385     246
 
AflIII A'CryG_T

Cuts at:      0     169    2336    2338    3150 
   Size:        169    2167       2     812

  Fragments arranged by size: 

                2167     812     169       2
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      67    3150 
   Size:         67    3083
 
AlwNI CAG_nnn'CTG

Cuts at:      0     628    2008    3150 
   Size:        628    1380    1142

  Fragments arranged by size: 

                1380    1142     628
 
ApoI r'AATT_y

Cuts at:      0    2245    2662    3150 
   Size:       2245     417     488

  Fragments arranged by size: 

                2245     488     417
 
AvaI C'yCGr_G

Cuts at:      0    1061    2533    3150 
   Size:       1061    1472     617

  Fragments arranged by size: 

                1472    1061     617
 
BamHI G'GATC_C

Cuts at:      0     566    3150 
   Size:        566    2584
 
BanII G_rGCy'C

Cuts at:      0      74     240     600     671    2771    3150 
   Size:         74     166     360      71    2100     379

  Fragments arranged by size: 

                2100     379     360     166      74      71
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1880    2049    2480    2682    3150 
   Size:       1880     169     431     202     468

  Fragments arranged by size: 

                1880     468     431     202     169
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1602    1929    3150 
   Size:       1602     327    1221

  Fragments arranged by size: 

                1602    1221     327
 
BfrBI ATG'CAT

Cuts at:      0     952    3150 
   Size:        952    2198
 
BglI GCCn_nnn'nGGC

Cuts at:      0     595    3150 
   Size:        595    2555
 
BglII A'GATC_T

Cuts at:      0    1097    3150 
   Size:       1097    2053
 
BlpI GC'TnA_GC

Cuts at:      0      68    2155    3150 
   Size:         68    2087     995

  Fragments arranged by size: 

                2087     995      68
 
Bme1580I G_kGCm'C

Cuts at:      0     383     992    1012    3105    3150 
   Size:        383     609      20    2093      45

  Fragments arranged by size: 

                2093     609     383      45      20
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     205     995    1041    1470    2832    3150 
   Size:        205     790      46     429    1362     318

  Fragments arranged by size: 

                1362     790     429     318     205      46
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     543    3150 
   Size:        543    2607
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     580    1127    1531    2095    3150 
   Size:        580     547     404     564    1055

  Fragments arranged by size: 

                1055     580     564     547     404
 
Bpu10I CC'TnA_GC

Cuts at:      0     510     578    3150 
   Size:        510      68    2572

  Fragments arranged by size: 

                2572     510      68
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2785    3150 
   Size:       2785     365
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      26    1106    1649    3150 
   Size:         26    1080     543    1501

  Fragments arranged by size: 

                1501    1080     543      26
 
BsaAI yAC'GTr

Cuts at:      0    2339    3150 
   Size:       2339     811
 
BsaBI GATnn'nnATC

Cuts at:      0    2315    2971    3001    3150 
   Size:       2315     656      30     149

  Fragments arranged by size: 

                2315     656     149      30
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     324     354    2618    2648    3150 
   Size:        324      30    2264      30     502

  Fragments arranged by size: 

                2264     502     324      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1124    2563    3040    3150 
   Size:       1124    1439     477     110

  Fragments arranged by size: 

                1439    1124     477     110
 
BseYI C'CCAG_C

Cuts at:      0     638    1618    3150 
   Size:        638     980    1532

  Fragments arranged by size: 

                1532     980     638
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     347    1715    3150 
   Size:        347    1368    1435

  Fragments arranged by size: 

                1435    1368     347
 
BsiEI CG_ry'CG

Cuts at:      0    2964    3150 
   Size:       2964     186
 
BsiHKAI G_wGCw'C

Cuts at:      0     240    2354    2771    3150 
   Size:        240    2114     417     379

  Fragments arranged by size: 

                2114     417     379     240
 
BsmI GAATG_Cn'

Cuts at:      0    2159    3150 
   Size:       2159     991
 
Bsp1286I G_dGCh'C

Cuts at:      0      74     240     383     600     671     992    1012    2354 
   Size:         74     166     143     217      71     321      20    1342

Cuts at:   2354    2771    3105    3150 
   Size:        417     334      45

  Fragments arranged by size: 

                1342     417     334     321     217     166     143      74
                  71      45      20
 
BspHI T'CATG_A

Cuts at:      0     353    1667    2706    2907    3150 
   Size:        353    1314    1039     201     243

  Fragments arranged by size: 

                1314    1039     353     243     201
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      59    2013    2054    3150 
   Size:         59    1954      41    1096

  Fragments arranged by size: 

                1954    1096      59      41
 
BsrDI GCAATG_nn'

Cuts at:      0     441    3150 
   Size:        441    2709
 
BsrFI r'CCGG_y

Cuts at:      0    2227    3036    3150 
   Size:       2227     809     114

  Fragments arranged by size: 

                2227     809     114
 
BssSI C'ACGA_G

Cuts at:      0    1858    3150 
   Size:       1858    1292
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2224    3150 
   Size:       2224     926
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      82    1860    3150 
   Size:         82    1778    1290

  Fragments arranged by size: 

                1778    1290      82
 
BstYI r'GATC_y

Cuts at:      0     566    1097    3150 
   Size:        566     531    2053

  Fragments arranged by size: 

                2053     566     531
 
BtgI C'CryG_G

Cuts at:      0     495    3150 
   Size:        495    2655
 
BtsI GCAGTG_nn'

Cuts at:      0     383    2225    3150 
   Size:        383    1842     925

  Fragments arranged by size: 

                1842     925     383
 
DraI TTT'AAA

Cuts at:      0    1077    1347    3150 
   Size:       1077     270    1803

  Fragments arranged by size: 

                1803    1077     270
 
DraIII CAC_nnn'GTG

Cuts at:      0     138    3150 
   Size:        138    3012
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     103    3150 
   Size:        103    3047
 
EaeI y'GGCC_r

Cuts at:      0     465    1047    1592    2087    2867    2984    3150 
   Size:        465     582     545     495     780     117     166

  Fragments arranged by size: 

                 780     582     545     495     465     166     117
 
EarI CTCTTCn'nnn_

Cuts at:      0     852    2776    2831    3150 
   Size:        852    1924      55     319

  Fragments arranged by size: 

                1924     852     319      55
 
EcoICRI GAG'CTC

Cuts at:      0     238    2769    3150 
   Size:        238    2531     381

  Fragments arranged by size: 

                2531     381     238
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     580    1127    1152    1531    2095    2519    2765    3150 
   Size:        580     547      25     379     564     424     246     385

  Fragments arranged by size: 

                 580     564     547     424     385     379     246      25
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1546    3150 
   Size:       1546    1604
 
EcoO109I rG'GnC_Cy

Cuts at:      0     554    3117    3150 
   Size:        554    2563      33

  Fragments arranged by size: 

                2563     554      33
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1116    1746    2484    2958    3150 
   Size:       1116     630     738     474     192

  Fragments arranged by size: 

                1116     738     630     474     192
 
HaeII r_GCGC'y

Cuts at:      0     803    3150 
   Size:        803    2347
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     324     356     676     708    1223    1255    1366    1398 
   Size:        324      32     320      32     515      32     111      32

Cuts at:   1398    1839    1871    2378    2410    3150 
   Size:        441      32     507      32     740

  Fragments arranged by size: 

                 740     515     507     441     324     320     111      32
                  32      32      32      32      32
 
HincII GTy'rAC

Cuts at:      0     849    1164    3150 
   Size:        849     315    1986

  Fragments arranged by size: 

                1986     849     315
 
HpaI GTT'AAC

Cuts at:      0     849    3150 
   Size:        849    2301
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1285    2115    3150 
   Size:       1285     830    1035

  Fragments arranged by size: 

                1285    1035     830
 
MscI TGG'CCA

Cuts at:      0     467    1049    1594    2089    2986    3150 
   Size:        467     582     545     495     897     164

  Fragments arranged by size: 

                 897     582     545     495     467     164
 
MslI CAynn'nnrTG

Cuts at:      0     262    1253    3150 
   Size:        262     991    1897

  Fragments arranged by size: 

                1897     991     262
 
MspA1I CmG'CkG

Cuts at:      0     312    3020    3150 
   Size:        312    2708     130

  Fragments arranged by size: 

                2708     312     130
 
NcoI C'CATG_G

Cuts at:      0     495    3150 
   Size:        495    2655
 
NsiI A_TGCA'T

Cuts at:      0     954    3150 
   Size:        954    2196
 
NspI r_CATG'y

Cuts at:      0     173     272    1819    3150 
   Size:        173      99    1547    1331

  Fragments arranged by size: 

                1547    1331     173      99
 
PasI CC'CwG_GG

Cuts at:      0    1366    3150 
   Size:       1366    1784
 
PciI A'CATG_T

Cuts at:      0     169    3150 
   Size:        169    2981
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     288    3150 
   Size:        288    2862
 
PfoI T'CCnGG_A

Cuts at:      0    1547    3150 
   Size:       1547    1603
 
PmlI CAC'GTG

Cuts at:      0    2339    3150 
   Size:       2339     811
 
PpuMI rG'GwC_Cy

Cuts at:      0     554    3150 
   Size:        554    2596
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    2800    2832    3150 
   Size:       2800      32     318

  Fragments arranged by size: 

                2800     318      32
 
PstI C_TGCA'G

Cuts at:      0      56    1299    2051    2996    3150 
   Size:         56    1243     752     945     154

  Fragments arranged by size: 

                1243     945     752     154      56
 
PvuI CG_AT'CG

Cuts at:      0    2964    3150 
   Size:       2964     186
 
PvuII CAG'CTG

Cuts at:      0     312    3150 
   Size:        312    2838
 
SacI G_AGCT'C

Cuts at:      0     240    2771    3150 
   Size:        240    2531     379

  Fragments arranged by size: 

                2531     379     240
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2776    3150 
   Size:       2776     374
 
SbfI CC_TGCA'GG

Cuts at:      0      56    3150 
   Size:         56    3094
 
ScaI AGT'ACT

Cuts at:      0    1628    2762    3031    3150 
   Size:       1628    1134     269     119

  Fragments arranged by size: 

                1628    1134     269     119
 
SexAI A'CCwGG_T

Cuts at:      0      33    3150 
   Size:         33    3117
 
SfcI C'TryA_G

Cuts at:      0      52    1295    2014    2047    2992    3150 
   Size:         52    1243     719      33     945     158

  Fragments arranged by size: 

                1243     945     719     158      52      33
 
SmaI CCC'GGG

Cuts at:      0    2535    3150 
   Size:       2535     615
 
SmlI C'TyrA_G

Cuts at:      0    2764    3150 
   Size:       2764     386
 
SspI AAT'ATT

Cuts at:      0     416    1663    3150 
   Size:        416    1247    1487

  Fragments arranged by size: 

                1487    1247     416
 
StyI C'CwwG_G

Cuts at:      0     185     495    1583    3150 
   Size:        185     310    1088    1567

  Fragments arranged by size: 

                1567    1088     310     185
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     317    2508    3150 
   Size:        317    2191     642

  Fragments arranged by size: 

                2191     642     317
 
TatI w'GTAC_w

Cuts at:      0    1125    1626    2760    3029    3077    3150 
   Size:       1125     501    1134     269      48      73

  Fragments arranged by size: 

                1134    1125     501     269      73      48
 
XbaI T'CTAG_A

Cuts at:      0     864    1434    3150 
   Size:        864     570    1716

  Fragments arranged by size: 

                1716     864     570
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     192     502    1602    2979    3150 
   Size:        192     310    1100    1377     171

  Fragments arranged by size: 

                1377    1100     310     192     171
 
XmaI C'CCGG_G

Cuts at:      0    2533    3150 
   Size:       2533     617
 
XmnI GAAnn'nnTTC

Cuts at:      0     520    1663    2056    2249    2903    3150 
   Size:        520    1143     393     193     654     247

  Fragments arranged by size: 

                1143     654     520     393     247     193
 

 Enzymes that do cut:

     AarI     AcuI   AflIII     AlfI    AlwNI     ApoI     AvaI    BamHI
    BanII     BbsI    BciVI    BfrBI     BglI    BglII     BlpI Bme1580I
     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I
    BspHI    BspMI    BsrDI    BsrFI    BssSI   BstAPI    BstXI    BstYI
     BtgI     BtsI     DraI   DraIII     DrdI     EaeI     EarI  EcoICRI
  Eco57MI    EcoNI EcoO109I     FalI    HaeII    Hin4I   HincII     HpaI
     MmeI     MscI     MslI   MspA1I     NcoI     NsiI     NspI     PasI
     PciI    PflMI     PfoI     PmlI    PpuMI     PsrI     PstI     PvuI
    PvuII     SacI     SapI     SbfI     ScaI    SexAI     SfcI     SmaI
     SmlI     SspI     StyI    TaqII     TatI     XbaI     XcmI     XmaI
     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI     BanI     BbeI    BbvCI     BcgI     BclI    BmgBI     BmtI
    BsaHI    BsaWI    BsiWI    BsmBI    BspEI    BsrBI    BsrGI   BssHII
    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     EagI
     EciI    EcoRI    EcoRV     FseI     FspI    FspAI  HindIII     KasI
     KpnI     MfeI     MluI     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     PacI     PmeI     PpiI    PshAI     PsiI   PspOMI
    PspXI    RsrII    SacII     SalI    SanDI     SfiI     SfoI     SgfI
    SgrAI    SnaBI     SpeI     SphI     SrfI     StuI     SwaI     TstI
  Tth111I     XhoI     ZraI
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