Restriction map for
pFN21ASDA0028
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# NO CUT ENZYMES ( 108 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AviII AvrII
BamHI BanII BbeI BbsI BcgI
BfrI BlnI BlpI Bpu1102I BpuAI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsiEI BsiHKAI BsiWI BsmI
BspDI BspEI BspHI BspLU11I BsrBI
BsrFI BssHII BssSI BstBI CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoT22I FauI FseI FspI
Hin1I KasI KpnI KspI MamI
MfeI MroI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SwaI XbaI XhoI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AocI 2506 CCTGAGG CC^T[ACGT]AGG
AspI 2590 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 931 CACGTG CAC^GTG
BciVI 1560 GGATAC GTATCC(6/5)
BglI 892 GCCTATACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2393 AGATCT A^GATCT
BsgI 289 CTGCAC GTGCAG(16/14)
BsmBI 1310 GAGACG CGTCTC(1/5)
Bsp1407I 1667 TGTACA T^GTACA
BsrGI 1667 TGTACA T^GTACA
Bst1107I 150 GTATAC GTA^TAC
BstEII 2214 GGTGACC G^GT[ACGT]ACC
BstPI 2214 GGTGACC G^GT[ACGT]ACC
BstZ17I 150 GTATAC GTA^TAC
Bsu36I 2506 CCTGAGG CC^T[ACGT]AGG
CfoI 2410 GCGC GCG^C
Eco81I 2506 CCTGAGG CC^T[ACGT]AGG
EcoO65I 2214 GGTGACC G^GT[ACGT]ACC
EcoRV 2023 GATATC GAT^ATC
HaeII 2409 GGCGCT [GA]GCGC^[CT]
HhaI 2410 GCGC GCG^C
HinP1I 2410 GCGC G^CGC
HincII 613 GTTAAC GT[CT]^[GA]AC
HindII 613 GTTAAC GT[CT]^[GA]AC
HindIII 2543 AAGCTT A^AGCTT
HpaI 613 GTTAAC GTT^AAC
MluI 761 ACGCGT A^CGCGT
PflFI 2590 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 931 CACGTG CAC^GTG
PmlI 931 CACGTG CAC^GTG
PstI 2497 CTGCAG CTGCA^G
ScaI 1132 AGTACT AGT^ACT
SmlI 990 CTTGAG C^T[CT][GA]AG
SphI 800 GCATGC GCATG^C
SspBI 1667 TGTACA T^GTACA
SspI 457 AATATT AAT^ATT
Tth111I 2590 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 31 enzymes) #
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AccI 150 GTATAC GT^[AC][GT]AC
310 GTCTAC
AccII 762 CGCG CG^CG
1452 CGCG
AflIII 526 ACGTGT A^C[GA][CT]GT
761 ACGCGT
AlwNI 224 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
2143 CAGCTGCTG
Asp700I 2602 GAATTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2658 GAAGTCCTTC
BanI 922 GGCACC G^G[CT][GA]CC
2415 GGTGCC
BbvCI 2140 CCTCAGC CCTCAGC(-5/-2)
2167 GCTGAGG
BclI 1737 TGATCA T^GATCA
2531 TGATCA
BfaI 1136 CTAG C^TAG
1270 CTAG
BspMI 360 GCAGGT ACCTGC(4/8)
654 GCAGGT
BsrDI 155 CATTGC GCAATG(2/0)
2279 GCAATG
BstAPI 1013 GCAAAGATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2470 GCATGGCCTGC
BstUI 762 CGCG CG^CG
1452 CGCG
BstXI 436 CCACAAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
810 CCACTGGATTGG
BstYI 1851 GGATCT [GA]^GATC[CT]
2393 AGATCT
EarI 38 CTCTTC CTCTTC(1/4)
1296 GAAGAG
EcoNI 1514 CCTGCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1791 CCTTATCAAGG
EcoRI 1124 GAATTC G^AATTC
2247 GAATTC
FbaI 1737 TGATCA T^GATCA
2531 TGATCA
Ksp632I 38 CTCTTC CTCTTC(1/4)
1296 GAAGAG
MaeI 1136 CTAG C^TAG
1270 CTAG
MflI 1851 GGATCT [GA]^GATC[CT]
2393 AGATCT
MvnI 762 CGCG CG^CG
1452 CGCG
NcoI 374 CCATGG C^CATGG
1487 CCATGG
PpuMI 620 GGGACCC [GA]G^G[AT]CC[CT]
1064 AGGTCCC
SmaI 1289 CCCGGG CCC^GGG
2579 CCCGGG
XcmI 647 CCAATGAGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1085 CCAAAGCTGACTTGG
XhoII 1851 GGATCT [GA]^GATC[CT]
2393 AGATCT
XmaCI 1289 CCCGGG C^CCGGG
2579 CCCGGG
XmaI 1289 CCCGGG C^CCGGG
2579 CCCGGG
XmnI 2602 GAATTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2658 GAAGTCCTTC
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# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaI AvaII BalI BbvI
BcnI BmyI BpmI BsaAI BsaJI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HgaI
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I