(Circular) (Six-base) MAPSORT of: pFN21ASDA0027.seq Check: 1590 from: 1 to: 5860 pFN21ASDA0027 5860 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3670 278 Size: 53 83 186 936 2134 2468 Fragments arranged by size: 2468 2134 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3203 1937 Size: 1266 4594 Acc65I G'GTAC_C Cuts at: 3181 3181 Size: 5860 AclI AA'CG_TT Cuts at: 1563 3987 4360 1563 Size: 2424 373 3063 Fragments arranged by size: 3063 2424 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2146 3119 3923 5307 5575 1632 Size: 514 973 804 1384 268 1917 Fragments arranged by size: 1917 1384 973 804 514 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5663 AflIII A'CryG_T Cuts at: 1203 2244 2393 4759 5736 1203 Size: 1041 149 2366 977 1327 Fragments arranged by size: 2366 1327 1041 977 149 AgeI A'CCGG_T Cuts at: 4691 4691 Size: 5860 AhdI GACnn_n'nnGTC Cuts at: 4589 4589 Size: 5860 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5860 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2305 1184 Size: 1121 4739 AlwNI CAG_nnn'CTG Cuts at: 2849 3603 5175 2849 Size: 754 1572 3534 Fragments arranged by size: 3534 1572 754 ApaLI G'TGCA_C Cuts at: 3917 5073 3917 Size: 1156 4704 ApoI r'AATT_y Cuts at: 1493 3169 3395 3547 5656 1493 Size: 1676 226 152 2109 1697 Fragments arranged by size: 2109 1697 1676 226 152 AseI AT'TA_AT Cuts at: 160 1032 4414 160 Size: 872 3382 1606 Fragments arranged by size: 3382 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5860 AvaI C'yCGr_G Cuts at: 1870 1943 2218 2598 2654 3185 1870 Size: 73 275 380 56 531 4545 Fragments arranged by size: 4545 531 380 275 73 56 BamHI G'GATC_C Cuts at: 2724 3190 3557 2724 Size: 466 367 5027 Fragments arranged by size: 5027 466 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2984 3181 4636 618 Size: 325 217 627 1197 197 1455 1842 Fragments arranged by size: 1842 1455 1197 627 325 217 197 BanII G_rGCy'C Cuts at: 1391 2552 3095 3179 1391 Size: 1161 543 84 4072 Fragments arranged by size: 4072 1161 543 84 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2173 928 Size: 971 274 4615 Fragments arranged by size: 4615 971 274 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2113 2131 2147 2165 4051 4085 1903 Size: 34 176 18 16 18 1886 34 3678 Fragments arranged by size: 3678 1886 176 34 34 18 18 16 BciVI GTATCCnnnnn_n' Cuts at: 3752 4962 3752 Size: 1210 4650 BclI T'GATC_A Cuts at: 1548 2502 1548 Size: 954 4906 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4471 136 Size: 107 122 71 631 605 2799 1525 Fragments arranged by size: 2799 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5855 5855 Size: 5860 BlpI GC'TnA_GC Cuts at: 3277 3277 Size: 5860 Bme1580I G_kGCm'C Cuts at: 1790 2987 3921 5077 1790 Size: 1197 934 1156 2573 Fragments arranged by size: 2573 1197 1156 934 BmgBI CAC'GTC Cuts at: 1318 2175 2826 1318 Size: 857 651 4352 Fragments arranged by size: 4352 857 651 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3145 3591 4549 Size: 416 53 83 399 24 1728 446 958 Cuts at: 4549 442 Size: 1753 Fragments arranged by size: 1753 1728 958 446 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5860 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2096 2308 2498 3145 4520 1239 Size: 857 212 190 647 1375 2579 Fragments arranged by size: 2579 1375 857 647 212 190 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3985 4850 5148 3985 Size: 865 298 4697 Fragments arranged by size: 4697 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4523 882 Size: 617 3024 2219 Fragments arranged by size: 3024 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2394 3157 3651 493 Size: 711 1190 763 494 2702 Fragments arranged by size: 2702 1190 763 711 494 BsaBI GATnn'nnATC Cuts at: 5854 5854 Size: 5860 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3667 4049 4684 275 Size: 53 83 186 936 2134 382 635 1451 Fragments arranged by size: 2134 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2061 4293 4691 4965 5112 5523 2061 Size: 2232 398 274 147 411 2398 Fragments arranged by size: 2398 2232 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5635 Fragments arranged by size: 5635 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2060 2540 1186 Size: 874 480 4506 Fragments arranged by size: 4506 874 480 BseYI C'CCAG_C Cuts at: 2780 3074 5063 5559 2780 Size: 294 1989 496 3081 Fragments arranged by size: 3081 1989 496 294 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2496 1163 Size: 573 760 4527 Fragments arranged by size: 4527 760 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3310 4071 5099 1540 Size: 275 186 1309 761 1028 2301 Fragments arranged by size: 2301 1309 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2958 3921 4006 5077 2958 Size: 963 85 1071 3741 Fragments arranged by size: 3741 1071 963 85 BsmI GAATG_Cn' Cuts at: 3371 3464 3371 Size: 93 5767 BsmBI CGTCTCn'nnnn_ Cuts at: 2326 2832 2326 Size: 506 5354 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2552 2958 2987 3095 3179 3921 4006 Size: 399 762 406 29 108 84 742 85 Cuts at: 4006 5077 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 762 742 406 399 108 85 84 29 BspEI T'CCGG_A Cuts at: 5523 5523 Size: 5860 BspHI T'CATG_A Cuts at: 2871 3749 5479 2871 Size: 878 1730 3252 Fragments arranged by size: 3252 1730 878 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2139 3215 844 Size: 1295 1076 3489 Fragments arranged by size: 3489 1295 1076 BsrBI CCG'CTC Cuts at: 1394 1823 2080 3747 1394 Size: 429 257 1667 3507 Fragments arranged by size: 3507 1667 429 257 BsrDI GCAATG_nn' Cuts at: 65 1248 2664 2751 4355 4529 65 Size: 1183 1416 87 1604 174 1396 Fragments arranged by size: 1604 1416 1396 1183 174 87 BsrFI r'CCGG_y Cuts at: 1700 1810 2085 2969 4504 4691 1700 Size: 110 275 884 1535 187 2869 Fragments arranged by size: 2869 1535 884 275 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5860 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5860 BssSI C'ACGA_G Cuts at: 3920 4932 3920 Size: 1012 4848 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5860 BstBI TT'CG_AA Cuts at: 2007 4664 2007 Size: 2657 3203 BstEII G'GTnAC_C Cuts at: 1184 2212 1184 Size: 1028 4832 BstXI CCAn_nnnn'nTGG Cuts at: 2575 2764 2575 Size: 189 5671 BstYI r'GATC_y Cuts at: 1972 2724 3190 3557 3942 3959 5411 5497 5509 Size: 752 466 367 385 17 1452 86 12 Cuts at: 5509 5855 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 752 466 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2496 513 Size: 552 1431 3877 Fragments arranged by size: 3877 1431 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2491 487 Size: 683 56 1265 3856 Fragments arranged by size: 3856 1265 683 56 BtsI GCAGTG_nn' Cuts at: 3376 4170 4190 3376 Size: 794 20 5046 Fragments arranged by size: 5046 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5860 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5825 DraI TTT'AAA Cuts at: 3164 3164 Size: 5860 DraIII CAC_nnn'GTG Cuts at: 2394 2394 Size: 5860 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4867 809 Size: 507 3551 1802 Fragments arranged by size: 3551 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2493 3084 3307 Size: 54 1425 50 275 18 663 591 223 Cuts at: 3307 4196 5825 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 663 591 275 223 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3307 1537 Size: 275 1495 4090 Fragments arranged by size: 4090 1495 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2138 3790 1353 Size: 785 1652 3423 Fragments arranged by size: 3423 1652 785 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4447 4821 4967 387 Size: 1064 138 2858 374 146 1280 Fragments arranged by size: 2858 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2096 2146 2308 2498 3119 3145 3923 Size: 393 464 50 162 190 621 26 778 Cuts at: 3923 4520 5307 5575 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 787 778 621 597 464 393 268 190 162 50 26 EcoNI CCTnn'n_nnAGG Cuts at: 2963 2963 Size: 5860 EcoO109I rG'GnC_Cy Cuts at: 2602 2800 2602 Size: 198 5662 EcoRI G'AATT_C Cuts at: 3169 3547 3169 Size: 378 5482 EcoRV GAT'ATC Cuts at: 2855 2855 Size: 5860 FseI GG_CCGG'CC Cuts at: 2973 2973 Size: 5860 FspI TGC'GCA Cuts at: 4366 4366 Size: 5860 HaeII r_GCGC'y Cuts at: 1678 2568 3070 5007 1678 Size: 890 502 1937 2531 Fragments arranged by size: 2531 1937 890 502 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4501 4533 4575 4607 1373 Size: 32 135 32 2929 32 42 32 2626 Fragments arranged by size: 2929 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3204 3450 669 Size: 988 281 1266 246 3079 Fragments arranged by size: 3079 1266 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5860 HpaI GTT'AAC Cuts at: 3450 3450 Size: 5860 KpnI G_GTAC'C Cuts at: 3185 3185 Size: 5860 MfeI C'AATT_G Cuts at: 2304 3459 2304 Size: 1155 4705 MluI A'CGCG_T Cuts at: 5736 5736 Size: 5860 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4974 5158 1305 Size: 3669 184 2007 Fragments arranged by size: 3669 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3086 10 Size: 54 1425 343 1254 2784 Fragments arranged by size: 2784 1425 1254 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3818 4177 4336 518 Size: 648 2652 359 159 2042 Fragments arranged by size: 2652 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2137 2833 3276 3953 5101 5346 1606 Size: 531 696 443 677 1148 245 2120 Fragments arranged by size: 2120 1148 696 677 531 443 245 NaeI GCC'GGC Cuts at: 2087 2971 2087 Size: 884 4976 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5308 NdeI CA'TA_TG Cuts at: 387 387 Size: 5860 NgoMIV G'CCGG_C Cuts at: 2085 2969 2085 Size: 884 4976 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5860 NotI GC'GGCC_GC Cuts at: 3307 3307 Size: 5860 NspI r_CATG'y Cuts at: 1132 1229 3218 4763 1132 Size: 97 1989 1545 2229 Fragments arranged by size: 2229 1989 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5860 PciI A'CATG_T Cuts at: 4759 4759 Size: 5860 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5860 PfoI T'CCnGG_A Cuts at: 2324 2324 Size: 5860 PmeI GTTT'AAAC Cuts at: 3164 3164 Size: 5860 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3929 3961 5442 5474 3929 Size: 32 1481 32 4315 Fragments arranged by size: 4315 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2602 2800 2602 Size: 198 5662 PshAI GACnn'nnGTC Cuts at: 1613 2177 1613 Size: 564 5296 PsiI TTA'TAA Cuts at: 3430 3430 Size: 5860 PstI C_TGCA'G Cuts at: 830 2136 2284 2967 3030 3212 830 Size: 1306 148 683 63 182 3478 Fragments arranged by size: 3478 1306 683 182 148 63 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5860 PvuII CAG'CTG Cuts at: 2137 2137 Size: 5860 SalI G'TCGA_C Cuts at: 1936 3202 1936 Size: 1266 4594 SbfI CC_TGCA'GG Cuts at: 3212 3212 Size: 5860 ScaI AGT'ACT Cuts at: 4108 5820 4108 Size: 1712 4148 SfcI C'TryA_G Cuts at: 826 1045 2132 2280 2963 3026 3208 4343 5024 Size: 219 1087 148 683 63 182 1135 681 Cuts at: 5024 5215 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1087 683 681 219 191 182 148 63 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5860 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5860 SmaI CCC'GGG Cuts at: 1872 3187 1872 Size: 1315 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3964 4865 5127 820 Size: 197 926 2021 901 262 1553 Fragments arranged by size: 2021 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3157 3651 493 Size: 2664 494 2702 Fragments arranged by size: 2702 2664 494 SpeI A'CTAG_T Cuts at: 152 2419 152 Size: 2267 3593 SphI G_CATG'C Cuts at: 1132 3218 1132 Size: 2086 3774 SspI AAT'ATT Cuts at: 5 52 3784 5 Size: 47 3732 2081 Fragments arranged by size: 3732 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5860 StyI C'CwwG_G Cuts at: 513 1065 3299 513 Size: 552 2234 3074 Fragments arranged by size: 3074 2234 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3062 3887 3904 4057 4242 4687 325 Size: 1255 1482 825 17 153 185 445 1498 Fragments arranged by size: 1498 1482 1255 825 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4106 5818 96 Size: 274 80 33 51 1443 2129 1712 138 Fragments arranged by size: 2129 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5820 5852 5820 Size: 32 5828 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5860 XbaI T'CTAG_A Cuts at: 3196 3196 Size: 5860 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2788 2788 Size: 5860 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5860 XmaI C'CCGG_G Cuts at: 1870 3185 1870 Size: 1315 4545 XmnI GAAnn'nnTTC Cuts at: 2246 3104 3989 2246 Size: 858 885 4117 Fragments arranged by size: 4117 885 858 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3668 276 Size: 53 83 186 936 2134 2468 Fragments arranged by size: 2468 2134 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I EcoICRI FalI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI