(Circular) (Six-base) MAPSORT of: pFN21ASDA0024.seq Check: 1117 from: 1 to: 6148 pFN21ASDA0024 6148 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3958 278 Size: 53 83 186 936 2422 2468 Fragments arranged by size: 2468 2422 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3491 1937 Size: 1554 4594 Acc65I G'GTAC_C Cuts at: 3469 3469 Size: 6148 AclI AA'CG_TT Cuts at: 1563 4275 4648 1563 Size: 2712 373 3063 Fragments arranged by size: 3063 2712 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3360 4211 5595 5863 1632 Size: 1728 851 1384 268 1917 Fragments arranged by size: 1917 1728 1384 851 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5951 AflIII A'CryG_T Cuts at: 1203 5047 6024 1203 Size: 3844 977 1327 Fragments arranged by size: 3844 1327 977 AgeI A'CCGG_T Cuts at: 4979 4979 Size: 6148 AhdI GACnn_n'nnGTC Cuts at: 4877 4877 Size: 6148 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6148 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6148 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3319 3351 3319 Size: 32 6116 AlwNI CAG_nnn'CTG Cuts at: 3891 5463 3891 Size: 1572 4576 ApaLI G'TGCA_C Cuts at: 4205 5361 4205 Size: 1156 4992 ApoI r'AATT_y Cuts at: 1493 2386 2991 3131 3457 3683 3835 5944 1493 Size: 893 605 140 326 226 152 2109 1697 Fragments arranged by size: 2109 1697 893 605 326 226 152 140 AseI AT'TA_AT Cuts at: 160 1032 4702 160 Size: 872 3670 1606 Fragments arranged by size: 3670 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6148 AvaI C'yCGr_G Cuts at: 1870 1943 3473 1870 Size: 73 1530 4545 Fragments arranged by size: 4545 1530 73 BamHI G'GATC_C Cuts at: 3478 3845 3478 Size: 367 5781 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3287 3469 4924 618 Size: 325 217 627 1500 182 1455 1842 Fragments arranged by size: 1842 1500 1455 627 325 217 182 BanII G_rGCy'C Cuts at: 1391 2625 3467 1391 Size: 1234 842 4072 Fragments arranged by size: 4072 1234 842 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2226 3348 928 Size: 971 327 1122 3728 Fragments arranged by size: 3728 1122 971 327 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4339 4373 1903 Size: 34 2402 34 3678 Fragments arranged by size: 3678 2402 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4040 5250 4040 Size: 1210 4938 BclI T'GATC_A Cuts at: 1548 2091 2487 2904 1548 Size: 543 396 417 4792 Fragments arranged by size: 4792 543 417 396 BfrBI ATG'CAT Cuts at: 2080 2635 2080 Size: 555 5593 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2558 4759 136 Size: 107 122 71 631 605 886 2201 1525 Fragments arranged by size: 2201 1525 886 631 605 122 107 71 BglII A'GATC_T Cuts at: 3142 3303 6143 3142 Size: 161 2840 3147 Fragments arranged by size: 3147 2840 161 BlpI GC'TnA_GC Cuts at: 2159 3565 2159 Size: 1406 4742 Bme1580I G_kGCm'C Cuts at: 1790 4209 5365 1790 Size: 2419 1156 2573 Fragments arranged by size: 2573 2419 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6148 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2770 3879 4837 Size: 416 53 83 399 24 1353 1109 958 Cuts at: 4837 442 Size: 1753 Fragments arranged by size: 1753 1353 1109 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2835 1056 Size: 1779 4369 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2258 2258 Size: 6148 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2251 2907 3131 4808 1239 Size: 1012 656 224 1677 2579 Fragments arranged by size: 2579 1677 1012 656 224 Bpu10I CC'TnA_GC Cuts at: 2051 2051 Size: 6148 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4273 5138 5436 4273 Size: 865 298 4985 Fragments arranged by size: 4985 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2588 4811 882 Size: 617 1089 2223 2219 Fragments arranged by size: 2223 2219 1089 617 BsaAI yAC'GTr Cuts at: 493 1204 3445 3939 493 Size: 711 2241 494 2702 Fragments arranged by size: 2702 2241 711 494 BsaBI GATnn'nnATC Cuts at: 2427 6142 2427 Size: 3715 2433 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2027 3955 4337 4972 Size: 53 83 186 936 494 1928 382 635 Cuts at: 4972 275 Size: 1451 Fragments arranged by size: 1928 1451 936 635 494 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2085 4581 4979 5253 5400 5811 2085 Size: 2496 398 274 147 411 2422 Fragments arranged by size: 2496 2422 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5923 Fragments arranged by size: 5923 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2630 1186 Size: 1444 4704 BseYI C'CCAG_C Cuts at: 2547 2716 3084 5351 5847 2547 Size: 169 368 2267 496 2848 Fragments arranged by size: 2848 2267 496 368 169 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5575 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2141 3598 4359 5387 1540 Size: 275 186 140 1457 761 1028 2301 Fragments arranged by size: 2301 1457 1028 761 275 186 140 BsiHKAI G_wGCw'C Cuts at: 2352 2625 4209 4294 5365 2352 Size: 273 1584 85 1071 3135 Fragments arranged by size: 3135 1584 1071 273 85 BsmI GAATG_Cn' Cuts at: 3403 3659 3752 3403 Size: 256 93 5799 Fragments arranged by size: 5799 256 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2352 2625 3467 4209 4294 5365 1391 Size: 399 562 273 842 742 85 1071 2174 Fragments arranged by size: 2174 1071 842 742 562 399 273 85 BspEI T'CCGG_A Cuts at: 2085 5811 2085 Size: 3726 2422 BspHI T'CATG_A Cuts at: 2901 4037 5767 2901 Size: 1136 1730 3282 Fragments arranged by size: 3282 1730 1136 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2386 3503 844 Size: 1542 1117 3489 Fragments arranged by size: 3489 1542 1117 BsrBI CCG'CTC Cuts at: 1394 1823 4035 1394 Size: 429 2212 3507 Fragments arranged by size: 3507 2212 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2704 4643 4817 65 Size: 1183 1456 1939 174 1396 Fragments arranged by size: 1939 1456 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2136 2733 4792 4979 1700 Size: 110 326 597 2059 187 2869 Fragments arranged by size: 2869 2059 597 326 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6148 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6148 BssSI C'ACGA_G Cuts at: 4208 5220 4208 Size: 1012 5136 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6148 BstBI TT'CG_AA Cuts at: 2007 4952 2007 Size: 2945 3203 BstEII G'GTnAC_C Cuts at: 1184 3251 1184 Size: 2067 4081 BstXI CCAn_nnnn'nTGG Cuts at: 2322 2982 2322 Size: 660 5488 BstYI r'GATC_y Cuts at: 1972 2422 3142 3303 3478 3845 4230 4247 5699 Size: 450 720 161 175 367 385 17 1452 Cuts at: 5699 5785 5797 6143 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 720 450 385 367 346 175 161 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 513 Size: 552 957 4639 Fragments arranged by size: 4639 957 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5409 Fragments arranged by size: 5409 683 56 BtsI GCAGTG_nn' Cuts at: 3664 4458 4478 3664 Size: 794 20 5334 Fragments arranged by size: 5334 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6148 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2686 2721 529 Size: 35 2122 35 3956 Fragments arranged by size: 3956 2122 35 35 DraI TTT'AAA Cuts at: 3452 3452 Size: 6148 DraIII CAC_nnn'GTG Cuts at: 3060 3060 Size: 6148 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5155 809 Size: 507 3839 1802 Fragments arranged by size: 3839 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2138 3595 4484 Size: 54 1425 50 275 18 308 1457 889 Cuts at: 4484 6113 8 Size: 1629 43 Fragments arranged by size: 1629 1457 1425 889 308 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2138 3595 1537 Size: 275 326 1457 4090 Fragments arranged by size: 4090 1457 326 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2384 2630 3152 4078 1353 Size: 1031 246 522 926 3423 Fragments arranged by size: 3423 1031 926 522 246 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4735 5109 5255 387 Size: 1064 138 3146 374 146 1280 Fragments arranged by size: 3146 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2623 2623 Size: 6148 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2251 2907 3131 3360 4211 4808 5595 Size: 393 619 656 224 229 851 597 787 Cuts at: 5595 5863 1239 Size: 268 1524 Fragments arranged by size: 1524 851 787 656 619 597 393 268 229 224 EcoNI CCTnn'n_nnAGG Cuts at: 2054 2197 2497 2054 Size: 143 300 5705 Fragments arranged by size: 5705 300 143 EcoRI G'AATT_C Cuts at: 2991 3457 3835 2991 Size: 466 378 5304 Fragments arranged by size: 5304 466 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2968 3399 2968 Size: 431 5717 FspI TGC'GCA Cuts at: 4654 4654 Size: 6148 HaeII r_GCGC'y Cuts at: 1678 5295 1678 Size: 3617 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2849 2881 3129 3134 3161 Size: 32 135 32 1277 32 248 5 27 Cuts at: 3161 3166 3382 3414 4789 4821 4863 4895 1373 Size: 5 216 32 1375 32 42 32 2626 Fragments arranged by size: 2626 1375 1277 248 216 135 42 32 32 32 32 32 32 27 5 5 HincII GTy'rAC Cuts at: 669 1657 1938 3492 3738 669 Size: 988 281 1554 246 3079 Fragments arranged by size: 3079 1554 988 281 246 HindIII A'AGCT_T Cuts at: 748 2767 748 Size: 2019 4129 HpaI GTT'AAC Cuts at: 3738 3738 Size: 6148 KpnI G_GTAC'C Cuts at: 3473 3473 Size: 6148 MfeI C'AATT_G Cuts at: 3747 3747 Size: 6148 MluI A'CGCG_T Cuts at: 6024 6024 Size: 6148 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3412 5262 5446 1305 Size: 2107 1850 184 2007 Fragments arranged by size: 2107 2007 1850 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4326 Fragments arranged by size: 4326 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2273 4106 4465 4624 518 Size: 648 1107 1833 359 159 2042 Fragments arranged by size: 2042 1833 1107 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2917 3564 4241 5389 5634 1606 Size: 1311 647 677 1148 245 2120 Fragments arranged by size: 2120 1311 1148 677 647 245 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 4639 Fragments arranged by size: 4639 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6148 NheI G'CTAG_C Cuts at: 1052 2831 1052 Size: 1779 4369 NotI GC'GGCC_GC Cuts at: 3595 3595 Size: 6148 NsiI A_TGCA'T Cuts at: 2082 2637 2082 Size: 555 5593 NspI r_CATG'y Cuts at: 1132 1229 3362 3506 5051 1132 Size: 97 2133 144 1545 2229 Fragments arranged by size: 2229 2133 1545 144 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6148 PciI A'CATG_T Cuts at: 5047 5047 Size: 6148 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6148 PfoI T'CCnGG_A Cuts at: 3108 3386 3108 Size: 278 5870 PmeI GTTT'AAAC Cuts at: 3452 3452 Size: 6148 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4217 4249 5730 5762 4217 Size: 32 1481 32 4603 Fragments arranged by size: 4603 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6148 PsiI TTA'TAA Cuts at: 3718 3718 Size: 6148 PstI C_TGCA'G Cuts at: 830 2820 3500 830 Size: 1990 680 3478 Fragments arranged by size: 3478 1990 680 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6148 PvuII CAG'CTG Cuts at: 2917 2917 Size: 6148 SacI G_AGCT'C Cuts at: 2625 2625 Size: 6148 SalI G'TCGA_C Cuts at: 1936 3490 1936 Size: 1554 4594 SapI GCTCTTCn'nnn_ Cuts at: 2630 2630 Size: 6148 SbfI CC_TGCA'GG Cuts at: 3500 3500 Size: 6148 ScaI AGT'ACT Cuts at: 3035 4396 6108 3035 Size: 1361 1712 3075 Fragments arranged by size: 3075 1712 1361 SfcI C'TryA_G Cuts at: 826 1045 2816 3292 3496 4631 5312 5503 826 Size: 219 1771 476 204 1135 681 191 1471 Fragments arranged by size: 1771 1471 1135 681 476 219 204 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2558 2558 Size: 6148 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6148 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6148 SmaI CCC'GGG Cuts at: 1872 3475 1872 Size: 1603 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4252 5153 5415 820 Size: 197 926 2309 901 262 1553 Fragments arranged by size: 2309 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3445 3939 493 Size: 2952 494 2702 Fragments arranged by size: 2952 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6148 SphI G_CATG'C Cuts at: 1132 3362 3506 1132 Size: 2230 144 3774 Fragments arranged by size: 3774 2230 144 SspI AAT'ATT Cuts at: 5 52 4072 5 Size: 47 4020 2081 Fragments arranged by size: 4020 2081 47 StuI AGG'CCT Cuts at: 1517 2561 1517 Size: 1044 5104 StyI C'CwwG_G Cuts at: 513 1065 2022 3587 513 Size: 552 957 1565 3074 Fragments arranged by size: 3074 1565 957 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3076 3367 4175 4192 4345 4530 4975 Size: 1255 1496 291 808 17 153 185 445 Cuts at: 4975 325 Size: 1498 Fragments arranged by size: 1498 1496 1255 808 445 291 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2184 3033 4394 Size: 274 80 33 51 1443 207 849 1361 Cuts at: 4394 6106 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1361 849 274 207 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2331 2363 3280 3312 6108 6140 2331 Size: 32 917 32 2796 32 2339 Fragments arranged by size: 2796 2339 917 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6148 XbaI T'CTAG_A Cuts at: 3484 3484 Size: 6148 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3306 3306 Size: 6148 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6148 XmaI C'CCGG_G Cuts at: 1870 3473 1870 Size: 1603 4545 XmnI GAAnn'nnTTC Cuts at: 2933 4277 2933 Size: 1344 4804 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3956 276 Size: 53 83 186 936 2422 2468 Fragments arranged by size: 2468 2422 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BsiWI BsmBI BstZ17I Bsu36I EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfoI SrfI SwaI