Restriction map for
pFN21ASDA0019
(Linear) (Six-base) MAPSORT of: KIAA0019.seq Check: 9091 from: 1 to: 2484
KIAA0019.orf
With 182 enzymes: SgfI *
October 24, 2006 15:10 ..
AatII G_ACGT'C
Cuts at: 0 1478 2484
Size: 1478 1006
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1009 1114 2484
Size: 1009 105 1370
Fragments arranged by size:
1370 1009 105
AflII C'TTAA_G
Cuts at: 0 745 2484
Size: 745 1739
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 866 898 2484
Size: 866 32 1586
Fragments arranged by size:
1586 866 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1075 1564 2341 2484
Size: 1075 489 777 143
Fragments arranged by size:
1075 777 489 143
ApaI G_GGCC'C
Cuts at: 0 333 624 2420 2484
Size: 333 291 1796 64
Fragments arranged by size:
1796 333 291 64
ApoI r'AATT_y
Cuts at: 0 3 287 730 961 2484
Size: 3 284 443 231 1523
Fragments arranged by size:
1523 443 284 231 3
AvaI C'yCGr_G
Cuts at: 0 1273 1755 1868 2258 2484
Size: 1273 482 113 390 226
Fragments arranged by size:
1273 482 390 226 113
BanI G'GyrC_C
Cuts at: 0 1218 2484
Size: 1218 1266
BanII G_rGCy'C
Cuts at: 0 167 333 624 1189 1636 1729 2420 2484
Size: 167 166 291 565 447 93 691 64
Fragments arranged by size:
691 565 447 291 167 166 93 64
BbeI G_GCGC'C
Cuts at: 0 1222 2484
Size: 1222 1262
BbvCI CC'TCA_GC
Cuts at: 0 1616 2484
Size: 1616 868
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 592 626 2484
Size: 592 34 1858
Fragments arranged by size:
1858 592 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1806 2484
Size: 1806 678
BclI T'GATC_A
Cuts at: 0 2165 2484
Size: 2165 319
BfrBI ATG'CAT
Cuts at: 0 660 2484
Size: 660 1824
BglI GCCn_nnn'nGGC
Cuts at: 0 619 1110 2484
Size: 619 491 1374
Fragments arranged by size:
1374 619 491
BlpI GC'TnA_GC
Cuts at: 0 1769 2484
Size: 1769 715
Bme1580I G_kGCm'C
Cuts at: 0 333 624 1454 2420 2484
Size: 333 291 830 966 64
Fragments arranged by size:
966 830 333 291 64
BmtI G_CTAG'C
Cuts at: 0 1879 2270 2484
Size: 1879 391 214
Fragments arranged by size:
1879 391 214
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 292 2411 2484
Size: 292 2119 73
Fragments arranged by size:
2119 292 73
Bpu10I CC'TnA_GC
Cuts at: 0 1616 2484
Size: 1616 868
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 361 714 749 1173 2484
Size: 361 353 35 424 1311
Fragments arranged by size:
1311 424 361 353 35
BsaI GGTCTCn'nnnn_
Cuts at: 0 967 1998 2484
Size: 967 1031 486
Fragments arranged by size:
1031 967 486
BsaBI GATnn'nnATC
Cuts at: 0 1091 2484
Size: 1091 1393
BsaHI Gr'CG_yC
Cuts at: 0 1142 1219 1270 1475 2484
Size: 1142 77 51 205 1009
Fragments arranged by size:
1142 1009 205 77 51
BsaWI w'CCGG_w
Cuts at: 0 2040 2484
Size: 2040 444
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 866 896 1225 1255 2484
Size: 866 30 329 30 1229
Fragments arranged by size:
1229 866 329 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 172 175 577 1182 1324 1327 1711 2484
Size: 172 3 402 605 142 3 384 773
Fragments arranged by size:
773 605 402 384 172 142 3 3
BseYI C'CCAG_C
Cuts at: 0 1750 1850 2119 2484
Size: 1750 100 269 365
Fragments arranged by size:
1750 365 269 100
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 104 2484
Size: 104 2380
BsiEI CG_ry'CG
Cuts at: 0 1183 2378 2484
Size: 1183 1195 106
Fragments arranged by size:
1195 1183 106
BsiHKAI G_wGCw'C
Cuts at: 0 167 402 1241 1284 2484
Size: 167 235 839 43 1200
Fragments arranged by size:
1200 839 235 167 43
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1482 2230 2484
Size: 1482 748 254
Fragments arranged by size:
1482 748 254
Bsp1286I G_dGCh'C
Cuts at: 0 167 333 402 624 1189 1241 1284 1454
Size: 167 166 69 222 565 52 43 170
Cuts at: 1454 1636 1729 2420 2484
Size: 182 93 691 64
Fragments arranged by size:
691 565 222 182 170 167 166 93
69 64 52 43
BspEI T'CCGG_A
Cuts at: 0 2040 2484
Size: 2040 444
BspHI T'CATG_A
Cuts at: 0 711 2484
Size: 711 1773
BsrBI CCG'CTC
Cuts at: 0 1214 2484
Size: 1214 1270
BsrFI r'CCGG_y
Cuts at: 0 1178 2373 2484
Size: 1178 1195 111
Fragments arranged by size:
1195 1178 111
BssSI C'ACGA_G
Cuts at: 0 1234 2484
Size: 1234 1250
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2386 2484
Size: 2386 98
BstEII G'GTnAC_C
Cuts at: 0 2144 2484
Size: 2144 340
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1858 2484
Size: 1858 626
BstYI r'GATC_y
Cuts at: 0 344 2484
Size: 344 2140
BtgI C'CryG_G
Cuts at: 0 944 1508 1691 1914 2271 2322 2484
Size: 944 564 183 223 357 51 162
Fragments arranged by size:
944 564 357 223 183 162 51
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1628 2484
Size: 1628 856
BtsI GCAGTG_nn'
Cuts at: 0 1637 2484
Size: 1637 847
DraI TTT'AAA
Cuts at: 0 1815 2484
Size: 1815 669
DrdI GACnn_nn'nnGTC
Cuts at: 0 439 2484
Size: 439 2045
EaeI y'GGCC_r
Cuts at: 0 1180 1193 1591 2375 2484
Size: 1180 13 398 784 109
Fragments arranged by size:
1180 784 398 109 13
EagI C'GGCC_G
Cuts at: 0 1180 2375 2484
Size: 1180 1195 109
Fragments arranged by size:
1195 1180 109
EarI CTCTTCn'nnn_
Cuts at: 0 640 2484
Size: 640 1844
EcoICRI GAG'CTC
Cuts at: 0 165 2484
Size: 165 2319
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 292 1009 1114 2411 2484
Size: 292 717 105 1297 73
Fragments arranged by size:
1297 717 292 105 73
EcoNI CCTnn'n_nnAGG
Cuts at: 0 982 2484
Size: 982 1502
EcoO109I rG'GnC_Cy
Cuts at: 0 330 372 621 643 1561 1605 2416 2417
Size: 330 42 249 22 918 44 811 1
Cuts at: 2417 2484
Size: 67
Fragments arranged by size:
918 811 330 249 67 44 42 22
1
EcoRI G'AATT_C
Cuts at: 0 3 2484
Size: 3 2481
EcoRV GAT'ATC
Cuts at: 0 858 1843 2404 2484
Size: 858 985 561 80
Fragments arranged by size:
985 858 561 80
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 771 1122 2484
Size: 771 351 1362
Fragments arranged by size:
1362 771 351
FseI GG_CCGG'CC
Cuts at: 0 1182 2484
Size: 1182 1302
HaeII r_GCGC'y
Cuts at: 0 1222 1688 1705 2484
Size: 1222 466 17 779
Fragments arranged by size:
1222 779 466 17
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 308 340 2484
Size: 308 32 2144
Fragments arranged by size:
2144 308 32
HincII GTy'rAC
Cuts at: 0 444 2109 2484
Size: 444 1665 375
Fragments arranged by size:
1665 444 375
HindIII A'AGCT_T
Cuts at: 0 742 1324 2366 2484
Size: 742 582 1042 118
Fragments arranged by size:
1042 742 582 118
KasI G'GCGC_C
Cuts at: 0 1218 2484
Size: 1218 1266
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1823 2484
Size: 1823 661
MscI TGG'CCA
Cuts at: 0 1195 2484
Size: 1195 1289
MslI CAynn'nnrTG
Cuts at: 0 595 2484
Size: 595 1889
MspA1I CmG'CkG
Cuts at: 0 1191 1199 1402 1682 2484
Size: 1191 8 203 280 802
Fragments arranged by size:
1191 802 280 203 8
NaeI GCC'GGC
Cuts at: 0 1180 2484
Size: 1180 1304
NarI GG'CG_CC
Cuts at: 0 1219 2484
Size: 1219 1265
NcoI C'CATG_G
Cuts at: 0 944 1508 2322 2484
Size: 944 564 814 162
Fragments arranged by size:
944 814 564 162
NgoMIV G'CCGG_C
Cuts at: 0 1178 2484
Size: 1178 1306
NheI G'CTAG_C
Cuts at: 0 1875 2266 2484
Size: 1875 391 218
Fragments arranged by size:
1875 391 218
NsiI A_TGCA'T
Cuts at: 0 662 2484
Size: 662 1822
NspI r_CATG'y
Cuts at: 0 655 1738 2484
Size: 655 1083 746
Fragments arranged by size:
1083 746 655
PasI CC'CwG_GG
Cuts at: 0 1978 2484
Size: 1978 506
PflMI CCAn_nnn'nTGG
Cuts at: 0 1075 2155 2484
Size: 1075 1080 329
Fragments arranged by size:
1080 1075 329
PfoI T'CCnGG_A
Cuts at: 0 1999 2484
Size: 1999 485
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 866 898 2484
Size: 866 32 1586
Fragments arranged by size:
1586 866 32
PpuMI rG'GwC_Cy
Cuts at: 0 372 1561 2484
Size: 372 1189 923
Fragments arranged by size:
1189 923 372
PsiI TTA'TAA
Cuts at: 0 536 2484
Size: 536 1948
PspOMI G'GGCC_C
Cuts at: 0 329 620 2416 2484
Size: 329 291 1796 68
Fragments arranged by size:
1796 329 291 68
PstI C_TGCA'G
Cuts at: 0 2337 2484
Size: 2337 147
PvuII CAG'CTG
Cuts at: 0 1402 2484
Size: 1402 1082
SacI G_AGCT'C
Cuts at: 0 167 2484
Size: 167 2317
SexAI A'CCwGG_T
Cuts at: 0 1074 2484
Size: 1074 1410
SfcI C'TryA_G
Cuts at: 0 1484 1893 2333 2342 2439 2484
Size: 1484 409 440 9 97 45
Fragments arranged by size:
1484 440 409 97 45 9
SfoI GGC'GCC
Cuts at: 0 1220 2484
Size: 1220 1264
SmlI C'TyrA_G
Cuts at: 0 340 693 745 764 1152 2484
Size: 340 353 52 19 388 1332
Fragments arranged by size:
1332 388 353 340 52 19
SphI G_CATG'C
Cuts at: 0 1738 2484
Size: 1738 746
StyI C'CwwG_G
Cuts at: 0 97 675 944 1106 1508 2322 2484
Size: 97 578 269 162 402 814 162
Fragments arranged by size:
814 578 402 269 162 162 97
TatI w'GTAC_w
Cuts at: 0 1818 2073 2484
Size: 1818 255 411
Fragments arranged by size:
1818 411 255
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1225 1257 2484
Size: 1225 32 1227
Fragments arranged by size:
1227 1225 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1506 2484
Size: 1506 978
XmnI GAAnn'nnTTC
Cuts at: 0 275 881 965 2484
Size: 275 606 84 1519
Fragments arranged by size:
1519 606 275 84
ZraI GAC'GTC
Cuts at: 0 1476 2484
Size: 1476 1008
Enzymes that do cut:
AatII AcuI AflII AloI AlwNI ApaI ApoI AvaI
BanI BanII BbeI BbvCI BcgI BciVI BclI BfrBI
BglI BlpI Bme1580I BmtI BpmI Bpu10I BpuEI BsaI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BsrBI BsrFI BssSI
BstAPI BstEII BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FseI HaeII Hin4I HincII HindIII
KasI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NheI NsiI NspI PasI PflMI PfoI PpiI
PpuMI PsiI PspOMI PstI PvuII SacI SexAI SfcI
SfoI SmlI SphI StyI TatI TstI XcmI XmnI
ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflIII AgeI AhdI
AleI AlfI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbsI BglII BmgBI BmrI BplI BsaAI BsiWI
BsmI BspMI BsrDI BsrGI BssHII BstBI BstZ17I Bsu36I
ClaI CspCI DraIII EciI FspI FspAI HpaI KpnI
MfeI MluI NdeI NotI NruI PacI PciI PmeI
PmlI PshAI PspXI PsrI PvuI RsrII SacII SalI
SanDI SapI SbfI ScaI SfiI SgfI SgrAI SmaI
SnaBI SpeI SrfI SspI StuI SwaI TaqII Tth111I
XbaI XhoI XmaI