(Circular) (Six-base) MAPSORT of: pFN21ASDA0014.seq Check: 378 from: 1 to: 6208 pFN21ASDA0014 6208 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2044 2044 Size: 6208 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4018 278 Size: 53 83 186 936 2482 2468 Fragments arranged by size: 2482 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3551 1937 Size: 1614 4594 Acc65I G'GTAC_C Cuts at: 3210 3529 3210 Size: 319 5889 AclI AA'CG_TT Cuts at: 1563 4335 4708 1563 Size: 2772 373 3063 Fragments arranged by size: 3063 2772 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3478 4271 5655 5923 1632 Size: 1846 793 1384 268 1917 Fragments arranged by size: 1917 1846 1384 793 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6011 AflIII A'CryG_T Cuts at: 1203 2178 2749 5107 6084 1203 Size: 975 571 2358 977 1327 Fragments arranged by size: 2358 1327 977 975 571 AgeI A'CCGG_T Cuts at: 5039 5039 Size: 6208 AhdI GACnn_n'nnGTC Cuts at: 3144 4937 3144 Size: 1793 4415 AleI CACnn'nnGTG Cuts at: 1166 2033 1166 Size: 867 5341 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2615 1184 Size: 1431 4777 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2534 2566 2534 Size: 32 6176 AlwNI CAG_nnn'CTG Cuts at: 2059 2089 2212 2324 2495 2671 2770 3097 3951 Size: 30 123 112 171 176 99 327 854 Cuts at: 3951 5523 2059 Size: 1572 2744 Fragments arranged by size: 2744 1572 854 327 176 171 123 112 99 30 ApaLI G'TGCA_C Cuts at: 3302 4265 5421 3302 Size: 963 1156 4089 Fragments arranged by size: 4089 1156 963 ApoI r'AATT_y Cuts at: 1493 3517 3743 3895 6004 1493 Size: 2024 226 152 2109 1697 Fragments arranged by size: 2109 2024 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4762 160 Size: 872 3730 1606 Fragments arranged by size: 3730 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6208 AvaI C'yCGr_G Cuts at: 1870 1943 3533 1870 Size: 73 1590 4545 Fragments arranged by size: 4545 1590 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2165 2198 2165 Size: 33 6175 BamHI G'GATC_C Cuts at: 2410 3538 3905 2410 Size: 1128 367 4713 Fragments arranged by size: 4713 1128 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2417 3082 3210 3529 4984 Size: 325 217 627 630 665 128 319 1455 Cuts at: 4984 618 Size: 1842 Fragments arranged by size: 1842 1455 665 630 627 325 319 217 128 BanII G_rGCy'C Cuts at: 1391 2889 2900 2951 3020 3527 1391 Size: 1498 11 51 69 507 4072 Fragments arranged by size: 4072 1498 507 69 51 11 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2156 928 Size: 971 257 4980 Fragments arranged by size: 4980 971 257 BbvCI CC'TCA_GC Cuts at: 2628 2628 Size: 6208 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4399 4433 1903 Size: 34 2462 34 3678 Fragments arranged by size: 3678 2462 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4100 5310 4100 Size: 1210 4998 BclI T'GATC_A Cuts at: 1548 2739 1548 Size: 1191 5017 BfrBI ATG'CAT Cuts at: 2463 2463 Size: 6208 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2084 2727 3225 Size: 107 122 71 631 605 412 643 498 Cuts at: 3225 4819 136 Size: 1594 1525 Fragments arranged by size: 1594 1525 643 631 605 498 412 122 107 71 BglII A'GATC_T Cuts at: 6203 6203 Size: 6208 BlpI GC'TnA_GC Cuts at: 2888 2933 3285 3625 2888 Size: 45 352 340 5471 Fragments arranged by size: 5471 352 340 45 Bme1580I G_kGCm'C Cuts at: 1790 2230 2245 3306 3445 4269 5425 1790 Size: 440 15 1061 139 824 1156 2573 Fragments arranged by size: 2573 1156 1061 824 440 139 15 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6208 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3939 4897 442 Size: 416 53 83 399 24 2522 958 1753 Fragments arranged by size: 2522 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6208 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2975 2984 3152 3386 4868 1239 Size: 1736 9 168 234 1482 2579 Fragments arranged by size: 2579 1736 1482 234 168 9 Bpu10I CC'TnA_GC Cuts at: 2628 2628 Size: 6208 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2062 3020 3236 4333 5198 5496 2062 Size: 958 216 1097 865 298 2774 Fragments arranged by size: 2774 1097 958 865 298 216 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4871 882 Size: 617 3372 2219 Fragments arranged by size: 3372 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2181 2752 3505 3999 493 Size: 711 977 571 753 494 2702 Fragments arranged by size: 2702 977 753 711 571 494 BsaBI GATnn'nnATC Cuts at: 6202 6202 Size: 6208 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4015 4397 5032 275 Size: 53 83 186 936 2482 382 635 1451 Fragments arranged by size: 2482 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3207 4641 5039 5313 5460 5871 3207 Size: 1434 398 274 147 411 3544 Fragments arranged by size: 3544 1434 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2534 2564 1178 Size: 30 165 30 1131 30 4822 Fragments arranged by size: 4822 1131 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2890 3398 1186 Size: 1704 508 3996 Fragments arranged by size: 3996 1704 508 BseYI C'CCAG_C Cuts at: 2469 3162 5411 5907 2469 Size: 693 2249 496 2770 Fragments arranged by size: 2770 2249 693 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5635 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3658 4419 5447 1540 Size: 275 186 1657 761 1028 2301 Fragments arranged by size: 2301 1657 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2048 2889 2983 3306 3453 4269 4354 5425 2048 Size: 841 94 323 147 816 85 1071 2831 Fragments arranged by size: 2831 1071 841 816 323 147 94 85 BsmI GAATG_Cn' Cuts at: 3719 3812 3719 Size: 93 6115 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2048 2230 2245 2889 2900 2951 2983 Size: 399 258 182 15 644 11 51 32 Cuts at: 2983 3020 3306 3445 3453 3527 4269 4354 5425 Size: 37 286 139 8 74 742 85 1071 Cuts at: 5425 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 644 399 286 258 182 139 85 74 51 37 32 15 11 8 BspEI T'CCGG_A Cuts at: 5871 5871 Size: 6208 BspHI T'CATG_A Cuts at: 4097 5827 4097 Size: 1730 4478 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2044 2541 2628 2643 2709 3563 844 Size: 1200 497 87 15 66 854 3489 Fragments arranged by size: 3489 1200 854 497 87 66 15 BsrBI CCG'CTC Cuts at: 1394 1823 2257 4095 1394 Size: 429 434 1838 3507 Fragments arranged by size: 3507 1838 434 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2463 4703 4877 65 Size: 1183 1215 2240 174 1396 Fragments arranged by size: 2240 1396 1215 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4852 5039 1700 Size: 110 3042 187 2869 Fragments arranged by size: 3042 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6208 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6208 BssSI C'ACGA_G Cuts at: 4268 5280 4268 Size: 1012 5196 BstAPI GCAn_nnn'nTGC Cuts at: 794 2059 2362 3421 794 Size: 1265 303 1059 3581 Fragments arranged by size: 3581 1265 1059 303 BstBI TT'CG_AA Cuts at: 2007 5012 2007 Size: 3005 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6208 BstXI CCAn_nnnn'nTGG Cuts at: 2515 2515 Size: 6208 BstYI r'GATC_y Cuts at: 1972 2410 3538 3905 4290 4307 5759 5845 5857 Size: 438 1128 367 385 17 1452 86 12 Cuts at: 5857 6203 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1128 438 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2991 3433 2991 Size: 442 5766 BtgI C'CryG_G Cuts at: 513 1065 2514 2820 3240 3459 513 Size: 552 1449 306 420 219 3262 Fragments arranged by size: 3262 1449 552 420 306 219 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5469 Fragments arranged by size: 5469 683 56 BtsI GCAGTG_nn' Cuts at: 2069 2230 3724 4518 4538 2069 Size: 161 1494 794 20 3739 Fragments arranged by size: 3739 1494 794 161 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6208 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6173 DraI TTT'AAA Cuts at: 3512 3512 Size: 6208 DraIII CAC_nnn'GTG Cuts at: 2794 2794 Size: 6208 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5215 809 Size: 507 3899 1802 Fragments arranged by size: 3899 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2772 3171 3341 Size: 54 1425 50 275 18 942 399 170 Cuts at: 3341 3655 4544 6173 8 Size: 314 889 1629 43 Fragments arranged by size: 1629 1425 942 889 399 314 275 170 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3655 1537 Size: 275 1843 4090 Fragments arranged by size: 4090 1843 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2117 4138 1353 Size: 764 2021 3423 Fragments arranged by size: 3423 2021 764 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4795 5169 5315 387 Size: 1064 138 3206 374 146 1280 Fragments arranged by size: 3206 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2887 2887 Size: 6208 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2975 2984 3152 3386 3478 4271 4868 Size: 393 1343 9 168 234 92 793 597 Cuts at: 4868 5655 5923 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1343 793 787 597 393 268 234 168 92 9 EcoNI CCTnn'n_nnAGG Cuts at: 2249 2567 3365 2249 Size: 318 798 5092 Fragments arranged by size: 5092 798 318 EcoO109I rG'GnC_Cy Cuts at: 2086 2617 2632 2995 2086 Size: 531 15 363 5299 Fragments arranged by size: 5299 531 363 15 EcoRI G'AATT_C Cuts at: 3517 3895 3517 Size: 378 5830 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2152 2152 Size: 6208 FspI TGC'GCA Cuts at: 2645 4714 2645 Size: 2069 4139 HaeII r_GCGC'y Cuts at: 1678 5355 1678 Size: 3677 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3130 3162 4849 4881 4923 Size: 32 135 32 1558 32 1687 32 42 Cuts at: 4923 4955 1373 Size: 32 2626 Fragments arranged by size: 2626 1687 1558 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3552 3798 669 Size: 988 281 1614 246 3079 Fragments arranged by size: 3079 1614 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6208 HpaI GTT'AAC Cuts at: 3798 3798 Size: 6208 KpnI G_GTAC'C Cuts at: 3214 3533 3214 Size: 319 5889 MfeI C'AATT_G Cuts at: 3807 3807 Size: 6208 MluI A'CGCG_T Cuts at: 6084 6084 Size: 6208 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3024 5322 5506 1305 Size: 1719 2298 184 2007 Fragments arranged by size: 2298 2007 1719 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2774 3173 10 Size: 54 1425 343 942 399 3045 Fragments arranged by size: 3045 1425 942 399 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2033 2426 2513 4166 4525 4684 518 Size: 648 867 393 87 1653 359 159 2042 Fragments arranged by size: 2042 1653 867 648 393 359 159 87 MspA1I CmG'CkG Cuts at: 1606 2075 2194 2212 2599 2929 3079 3166 3421 Size: 469 119 18 387 330 150 87 255 Cuts at: 3421 3624 4301 5449 5694 1606 Size: 203 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 469 387 330 255 245 203 150 119 87 18 NcoI C'CATG_G Cuts at: 513 1065 3240 513 Size: 552 2175 3481 Fragments arranged by size: 3481 2175 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6208 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6208 NotI GC'GGCC_GC Cuts at: 3655 3655 Size: 6208 NsiI A_TGCA'T Cuts at: 2465 2465 Size: 6208 NspI r_CATG'y Cuts at: 1132 1229 2278 2360 2431 2463 3026 3566 5111 Size: 97 1049 82 71 32 563 540 1545 Cuts at: 5111 1132 Size: 2229 Fragments arranged by size: 2229 1545 1049 563 540 97 82 71 32 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6208 PciI A'CATG_T Cuts at: 5107 5107 Size: 6208 PflMI CCAn_nnn'nTGG Cuts at: 1108 2770 3169 1108 Size: 1662 399 4147 Fragments arranged by size: 4147 1662 399 PmeI GTTT'AAAC Cuts at: 3512 3512 Size: 6208 PmlI CAC'GTG Cuts at: 2181 2752 2181 Size: 571 5637 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2534 2566 4277 4309 5790 5822 2534 Size: 32 1711 32 1481 32 2920 Fragments arranged by size: 2920 1711 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2617 2632 2995 2617 Size: 15 363 5830 Fragments arranged by size: 5830 363 15 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6208 PsiI TTA'TAA Cuts at: 3778 3778 Size: 6208 PstI C_TGCA'G Cuts at: 830 2064 2237 2253 2351 2667 2946 3560 830 Size: 1234 173 16 98 316 279 614 3478 Fragments arranged by size: 3478 1234 614 316 279 173 98 16 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6208 PvuII CAG'CTG Cuts at: 2075 2212 2929 3079 3166 3421 2075 Size: 137 717 150 87 255 4862 Fragments arranged by size: 4862 717 255 150 137 87 SacI G_AGCT'C Cuts at: 2889 2889 Size: 6208 SalI G'TCGA_C Cuts at: 1936 3550 1936 Size: 1614 4594 SbfI CC_TGCA'GG Cuts at: 2253 3560 2253 Size: 1307 4901 ScaI AGT'ACT Cuts at: 4456 6168 4456 Size: 1712 4496 SfcI C'TryA_G Cuts at: 826 1045 2060 2216 2233 2249 2347 2663 2942 Size: 219 1015 156 17 16 98 316 279 Cuts at: 2942 3556 4691 5372 5563 826 Size: 614 1135 681 191 1471 Fragments arranged by size: 1471 1135 1015 681 614 316 279 219 191 156 98 17 16 SfiI GGCCn_nnn'nGGCC Cuts at: 2727 2727 Size: 6208 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6208 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6208 SmaI CCC'GGG Cuts at: 1872 3535 1872 Size: 1663 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2041 3035 3251 4312 5213 5475 Size: 197 926 98 994 216 1061 901 262 Cuts at: 5475 820 Size: 1553 Fragments arranged by size: 1553 1061 994 926 901 262 216 197 98 SnaBI TAC'GTA Cuts at: 493 3505 3999 493 Size: 3012 494 2702 Fragments arranged by size: 3012 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6208 SphI G_CATG'C Cuts at: 1132 2278 2360 3566 1132 Size: 1146 82 1206 3774 Fragments arranged by size: 3774 1206 1146 82 SspI AAT'ATT Cuts at: 5 52 4132 5 Size: 47 4080 2081 Fragments arranged by size: 4080 2081 47 StuI AGG'CCT Cuts at: 1517 3371 1517 Size: 1854 4354 StyI C'CwwG_G Cuts at: 513 1065 3214 3240 3647 513 Size: 552 2149 26 407 3074 Fragments arranged by size: 3074 2149 552 407 26 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3176 4235 4252 4405 4590 5035 325 Size: 1255 1596 1059 17 153 185 445 1498 Fragments arranged by size: 1596 1498 1255 1059 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2438 4454 6166 Size: 274 80 33 51 1443 461 2016 1712 Cuts at: 6166 96 Size: 138 Fragments arranged by size: 2016 1712 1443 461 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3168 3200 6168 6200 3168 Size: 32 2968 32 3176 Fragments arranged by size: 3176 2968 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6208 XbaI T'CTAG_A Cuts at: 3544 3544 Size: 6208 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2477 2795 2477 Size: 318 5890 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6208 XmaI C'CCGG_G Cuts at: 1870 3533 1870 Size: 1663 4545 XmnI GAAnn'nnTTC Cuts at: 4337 4337 Size: 6208 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4016 276 Size: 53 83 186 936 2482 2468 Fragments arranged by size: 2482 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BbeI BplI BsiWI BsmBI BstZ17I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfoI SrfI SwaI