Restriction map for
pFN21ASDA0007
(Linear) (Six-base) MAPSORT of: KIAA0007.seq Check: 1657 from: 1 to: 2031
KIAA0007.orf
With 182 enzymes: SgfI *
October 24, 2006 15:10 ..
AccI GT'mk_AC
Cuts at: 0 599 2031
Size: 599 1432
Acc65I G'GTAC_C
Cuts at: 0 178 1632 2031
Size: 178 1454 399
Fragments arranged by size:
1454 399 178
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1217 1832 1871 2031
Size: 1217 615 39 160
Fragments arranged by size:
1217 615 160 39
AgeI A'CCGG_T
Cuts at: 0 728 2031
Size: 728 1303
AhdI GACnn_n'nnGTC
Cuts at: 0 1032 2031
Size: 1032 999
AleI CACnn'nnGTG
Cuts at: 0 173 2031
Size: 173 1858
AlwNI CAG_nnn'CTG
Cuts at: 0 259 416 2031
Size: 259 157 1615
Fragments arranged by size:
1615 259 157
ApaI G_GGCC'C
Cuts at: 0 1146 2031
Size: 1146 885
ApoI r'AATT_y
Cuts at: 0 1191 2031
Size: 1191 840
AseI AT'TA_AT
Cuts at: 0 358 2031
Size: 358 1673
BanI G'GyrC_C
Cuts at: 0 178 201 1212 1632 2031
Size: 178 23 1011 420 399
Fragments arranged by size:
1011 420 399 178 23
BanII G_rGCy'C
Cuts at: 0 1146 2031
Size: 1146 885
BbeI G_GCGC'C
Cuts at: 0 205 2031
Size: 205 1826
BbsI GAAGACnn'nnnn_
Cuts at: 0 1370 1480 1756 1799 2031
Size: 1370 110 276 43 232
Fragments arranged by size:
1370 276 232 110 43
BfrBI ATG'CAT
Cuts at: 0 1603 2031
Size: 1603 428
BglI GCCn_nnn'nGGC
Cuts at: 0 210 2031
Size: 210 1821
Bme1580I G_kGCm'C
Cuts at: 0 1146 2031
Size: 1146 885
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 734 1080 1354 2031
Size: 734 346 274 677
Fragments arranged by size:
734 677 346 274
BsaI GGTCTCn'nnnn_
Cuts at: 0 580 2031
Size: 580 1451
BsaAI yAC'GTr
Cuts at: 0 156 2031
Size: 156 1875
BsaHI Gr'CG_yC
Cuts at: 0 202 2031
Size: 202 1829
BsaWI w'CCGG_w
Cuts at: 0 728 2031
Size: 728 1303
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1244 1831 1888 1891 2031
Size: 1244 587 57 3 140
Fragments arranged by size:
1244 587 140 57 3
BseYI C'CCAG_C
Cuts at: 0 43 1139 1637 1641 2031
Size: 43 1096 498 4 390
Fragments arranged by size:
1096 498 390 43 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 168 797 927 2031
Size: 127 41 629 130 1104
Fragments arranged by size:
1104 629 130 127 41
BsmBI CGTCTCn'nnnn_
Cuts at: 0 118 2031
Size: 118 1913
Bsp1286I G_dGCh'C
Cuts at: 0 1146 2031
Size: 1146 885
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 190 738 2031
Size: 190 548 1293
Fragments arranged by size:
1293 548 190
BsrBI CCG'CTC
Cuts at: 0 1252 2031
Size: 1252 779
BsrDI GCAATG_nn'
Cuts at: 0 1337 2031
Size: 1337 694
BsrFI r'CCGG_y
Cuts at: 0 137 728 2031
Size: 137 591 1303
Fragments arranged by size:
1303 591 137
BstYI r'GATC_y
Cuts at: 0 2004 2031
Size: 2004 27
BtsI GCAGTG_nn'
Cuts at: 0 513 785 2031
Size: 513 272 1246
Fragments arranged by size:
1246 513 272
DraI TTT'AAA
Cuts at: 0 1091 2031
Size: 1091 940
DrdI GACnn_nn'nnGTC
Cuts at: 0 382 2031
Size: 382 1649
EaeI y'GGCC_r
Cuts at: 0 128 2031
Size: 128 1903
EarI CTCTTCn'nnn_
Cuts at: 0 839 1796 1808 1904 2031
Size: 839 957 12 96 127
Fragments arranged by size:
957 839 127 96 12
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 734 1080 1217 1354 1832 1871 2031
Size: 734 346 137 137 478 39 160
Fragments arranged by size:
734 478 346 160 137 137 39
EcoNI CCTnn'n_nnAGG
Cuts at: 0 968 2031
Size: 968 1063
EcoO109I rG'GnC_Cy
Cuts at: 0 48 1143 2031
Size: 48 1095 888
Fragments arranged by size:
1095 888 48
EcoRI G'AATT_C
Cuts at: 0 1191 2031
Size: 1191 840
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 907 2031
Size: 907 1124
HaeII r_GCGC'y
Cuts at: 0 205 2031
Size: 205 1826
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1018 1050 1212 1244 2031
Size: 1018 32 162 32 787
Fragments arranged by size:
1018 787 162 32 32
HincII GTy'rAC
Cuts at: 0 387 983 1592 2031
Size: 387 596 609 439
Fragments arranged by size:
609 596 439 387
HindIII A'AGCT_T
Cuts at: 0 1711 2031
Size: 1711 320
HpaI GTT'AAC
Cuts at: 0 1592 2031
Size: 1592 439
KasI G'GCGC_C
Cuts at: 0 201 2031
Size: 201 1830
KpnI G_GTAC'C
Cuts at: 0 182 1636 2031
Size: 182 1454 395
Fragments arranged by size:
1454 395 182
MslI CAynn'nnrTG
Cuts at: 0 173 1703 2031
Size: 173 1530 328
Fragments arranged by size:
1530 328 173
MspA1I CmG'CkG
Cuts at: 0 556 1641 2031
Size: 556 1085 390
Fragments arranged by size:
1085 556 390
NarI GG'CG_CC
Cuts at: 0 202 2031
Size: 202 1829
NdeI CA'TA_TG
Cuts at: 0 1109 2031
Size: 1109 922
NsiI A_TGCA'T
Cuts at: 0 1605 2031
Size: 1605 426
NspI r_CATG'y
Cuts at: 0 473 622 2031
Size: 473 149 1409
Fragments arranged by size:
1409 473 149
PasI CC'CwG_GG
Cuts at: 0 1401 2031
Size: 1401 630
PflMI CCAn_nnn'nTGG
Cuts at: 0 1662 2031
Size: 1662 369
PpuMI rG'GwC_Cy
Cuts at: 0 48 2031
Size: 48 1983
PsiI TTA'TAA
Cuts at: 0 1468 2031
Size: 1468 563
PspOMI G'GGCC_C
Cuts at: 0 1142 2031
Size: 1142 889
PstI C_TGCA'G
Cuts at: 0 218 2031
Size: 218 1813
PvuII CAG'CTG
Cuts at: 0 556 1641 2031
Size: 556 1085 390
Fragments arranged by size:
1085 556 390
SanDI GG'GwC_CC
Cuts at: 0 48 2031
Size: 48 1983
SapI GCTCTTCn'nnn_
Cuts at: 0 839 2031
Size: 839 1192
ScaI AGT'ACT
Cuts at: 0 1442 2031
Size: 1442 589
SexAI A'CCwGG_T
Cuts at: 0 1628 2031
Size: 1628 403
SfcI C'TryA_G
Cuts at: 0 214 260 600 2031
Size: 214 46 340 1431
Fragments arranged by size:
1431 340 214 46
SfoI GGC'GCC
Cuts at: 0 203 2031
Size: 203 1828
StuI AGG'CCT
Cuts at: 0 79 2031
Size: 79 1952
StyI C'CwwG_G
Cuts at: 0 221 2031
Size: 221 1810
TatI w'GTAC_w
Cuts at: 0 720 1440 2031
Size: 720 720 591
Fragments arranged by size:
720 720 591
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 682 2031
Size: 682 1349
Enzymes that do cut:
AccI Acc65I AcuI AgeI AhdI AleI AlwNI ApaI
ApoI AseI BanI BanII BbeI BbsI BfrBI BglI
Bme1580I BpmI BsaI BsaAI BsaHI BsaWI BseRI BseYI
BsgI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BstYI
BtsI DraI DrdI EaeI EarI Eco57MI EcoNI EcoO109I
EcoRI FalI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MslI MspA1I NarI NdeI NsiI NspI PasI
PflMI PpuMI PsiI PspOMI PstI PvuII SanDI SapI
ScaI SexAI SfcI SfoI StuI StyI TatI XcmI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AflIII AlfI AloI
ApaLI AscI AsiSI AvaI AvrII BaeI BamHI BbvCI
BcgI BciVI BclI BglII BlpI BmgBI BmrI BmtI
BplI Bpu10I BpuEI BsaBI BsaXI BsiEI BsiHKAI BsiWI
BsmI BspEI BspHI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI
DraIII EagI EciI EcoICRI EcoRV FseI FspI FspAI
MfeI MluI MmeI MscI NaeI NcoI NgoMIV NheI
NotI NruI PacI PciI PfoI PmeI PmlI PpiI
PshAI PspXI PsrI PvuI RsrII SacI SacII SalI
SbfI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI SwaI TaqII TstI Tth111I XbaI
XhoI XmaI XmnI ZraI