(Circular) (Six-base) MAPSORT of: pFN21AB8006.seq Check: 3070 from: 1 to: 7024 pFN21AB8006 7024 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2320 3259 2320 Size: 939 6085 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4834 278 Size: 53 83 186 936 3298 2468 Fragments arranged by size: 3298 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3828 4367 1937 Size: 1891 539 4594 Fragments arranged by size: 4594 1891 539 Acc65I G'GTAC_C Cuts at: 4345 4345 Size: 7024 AclI AA'CG_TT Cuts at: 1563 5151 5524 1563 Size: 3588 373 3063 Fragments arranged by size: 3588 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3780 4031 5087 6471 6739 1632 Size: 2148 251 1056 1384 268 1917 Fragments arranged by size: 2148 1917 1384 1056 268 251 AfeI AGC'GCT Cuts at: 2091 2091 Size: 7024 AflII C'TTAA_G Cuts at: 820 1017 2339 2348 820 Size: 197 1322 9 5496 Fragments arranged by size: 5496 1322 197 9 AflIII A'CryG_T Cuts at: 1203 3912 4063 5923 6900 1203 Size: 2709 151 1860 977 1327 Fragments arranged by size: 2709 1860 1327 977 151 AgeI A'CCGG_T Cuts at: 5855 5855 Size: 7024 AhdI GACnn_n'nnGTC Cuts at: 2824 5753 2824 Size: 2929 4095 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7024 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2328 4182 1184 Size: 1144 1854 4026 Fragments arranged by size: 4026 1854 1144 AlwNI CAG_nnn'CTG Cuts at: 3254 4054 4767 6339 3254 Size: 800 713 1572 3939 Fragments arranged by size: 3939 1572 800 713 ApaI G_GGCC'C Cuts at: 2046 3216 3262 2046 Size: 1170 46 5808 Fragments arranged by size: 5808 1170 46 ApaLI G'TGCA_C Cuts at: 4309 5081 6237 4309 Size: 772 1156 5096 Fragments arranged by size: 5096 1156 772 ApoI r'AATT_y Cuts at: 1493 2710 3320 3374 4333 4559 4711 6820 1493 Size: 1217 610 54 959 226 152 2109 1697 Fragments arranged by size: 2109 1697 1217 959 610 226 152 54 AseI AT'TA_AT Cuts at: 160 1032 5578 160 Size: 872 4546 1606 Fragments arranged by size: 4546 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7024 AvaI C'yCGr_G Cuts at: 1870 1943 2976 3208 3314 3711 3788 4349 1870 Size: 73 1033 232 106 397 77 561 4545 Fragments arranged by size: 4545 1033 561 397 232 106 77 73 BamHI G'GATC_C Cuts at: 2237 4354 4721 2237 Size: 2117 367 4540 Fragments arranged by size: 4540 2117 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3632 4345 5800 618 Size: 325 217 627 1845 713 1455 1842 Fragments arranged by size: 1845 1842 1455 713 627 325 217 BanII G_rGCy'C Cuts at: 1391 2024 2046 2077 3216 3262 4305 4343 1391 Size: 633 22 31 1139 46 1043 38 4072 Fragments arranged by size: 4072 1139 1043 633 46 38 31 22 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6053 BbvCI CC'TCA_GC Cuts at: 2193 3243 4199 4233 2193 Size: 1050 956 34 4984 Fragments arranged by size: 4984 1050 956 34 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2171 2205 2729 2763 3389 3423 5215 Size: 34 234 34 524 34 626 34 1792 Cuts at: 5215 5249 1903 Size: 34 3678 Fragments arranged by size: 3678 1792 626 524 234 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4916 6126 4916 Size: 1210 5814 BclI T'GATC_A Cuts at: 1548 1548 Size: 7024 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3257 5635 136 Size: 107 122 71 631 605 1585 2378 1525 Fragments arranged by size: 2378 1585 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7019 7019 Size: 7024 BlpI GC'TnA_GC Cuts at: 3390 4441 3390 Size: 1051 5973 Bme1580I G_kGCm'C Cuts at: 1790 2046 2776 3216 3262 4313 5085 6241 1790 Size: 256 730 440 46 1051 772 1156 2573 Fragments arranged by size: 2573 1156 1051 772 730 440 256 46 BmgBI CAC'GTC Cuts at: 1318 2434 2479 3915 4321 1318 Size: 1116 45 1436 406 4021 Fragments arranged by size: 4021 1436 1116 406 45 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4084 4755 5713 Size: 416 53 83 399 24 2667 671 958 Cuts at: 5713 442 Size: 1753 Fragments arranged by size: 2667 1753 958 671 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 4232 1056 Size: 3176 3848 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2041 2043 2091 3208 2041 Size: 2 48 1117 5857 Fragments arranged by size: 5857 1117 48 2 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2967 3188 3356 5684 1239 Size: 1728 221 168 2328 2579 Fragments arranged by size: 2579 2328 1728 221 168 Bpu10I CC'TnA_GC Cuts at: 2193 2459 3149 3243 4199 4233 2193 Size: 266 690 94 956 34 4984 Fragments arranged by size: 4984 956 690 266 94 34 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3560 5149 6014 6312 3560 Size: 1589 865 298 4272 Fragments arranged by size: 4272 1589 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5687 882 Size: 617 4188 2219 Fragments arranged by size: 4188 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4815 493 Size: 711 3611 2702 Fragments arranged by size: 3611 2702 711 BsaBI GATnn'nnATC Cuts at: 7018 7018 Size: 7024 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4280 4831 5213 5848 Size: 53 83 186 936 2747 551 382 635 Cuts at: 5848 275 Size: 1451 Fragments arranged by size: 2747 1451 936 635 551 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2319 5457 5855 6129 6276 6687 2319 Size: 3138 398 274 147 411 2656 Fragments arranged by size: 3138 2656 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2043 2073 2494 2524 2940 Size: 30 165 30 640 30 421 30 416 Cuts at: 2940 2970 1178 Size: 30 5232 Fragments arranged by size: 5232 640 421 416 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2249 2570 3030 4229 4259 1186 Size: 1063 321 460 1199 30 3951 Fragments arranged by size: 3951 1199 1063 460 321 30 BseYI C'CCAG_C Cuts at: 3848 4287 6227 6723 3848 Size: 439 1940 496 4149 Fragments arranged by size: 4149 1940 496 439 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2160 2867 3536 4040 4175 1163 Size: 573 424 707 669 504 135 4012 Fragments arranged by size: 4012 707 669 573 504 424 135 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4474 5235 6263 1540 Size: 275 186 2473 761 1028 2301 Fragments arranged by size: 2473 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2077 2601 2880 4313 5085 5170 6241 2077 Size: 524 279 1433 772 85 1071 2860 Fragments arranged by size: 2860 1433 1071 772 524 279 85 BsmI GAATG_Cn' Cuts at: 4535 4628 4535 Size: 93 6931 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2024 2046 2077 2601 2776 2880 3216 Size: 399 234 22 31 524 175 104 336 Cuts at: 3216 3262 4305 4313 4343 5085 5170 6241 1391 Size: 46 1043 8 30 742 85 1071 2174 Fragments arranged by size: 2174 1071 1043 742 524 399 336 234 175 104 85 46 31 30 22 8 BspEI T'CCGG_A Cuts at: 2319 6687 2319 Size: 4368 2656 BspHI T'CATG_A Cuts at: 2928 4913 6643 2928 Size: 1985 1730 3309 Fragments arranged by size: 3309 1985 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2320 3259 4197 4379 844 Size: 1476 939 938 182 3489 Fragments arranged by size: 3489 1476 939 938 182 BsrBI CCG'CTC Cuts at: 1394 1823 2032 3394 4911 1394 Size: 429 209 1362 1517 3507 Fragments arranged by size: 3507 1517 1362 429 209 BsrDI GCAATG_nn' Cuts at: 65 1248 5519 5693 65 Size: 1183 4271 174 1396 Fragments arranged by size: 4271 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5668 5855 1700 Size: 110 3858 187 2869 Fragments arranged by size: 3858 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2412 96 Size: 2316 4708 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7024 BssSI C'ACGA_G Cuts at: 3554 3878 5084 6096 3554 Size: 324 1206 1012 4482 Fragments arranged by size: 4482 1206 1012 324 BstAPI GCAn_nnn'nTGC Cuts at: 794 3988 794 Size: 3194 3830 BstBI TT'CG_AA Cuts at: 3325 5828 3325 Size: 2503 4521 BstEII G'GTnAC_C Cuts at: 1184 2983 3640 1184 Size: 1799 657 4568 Fragments arranged by size: 4568 1799 657 BstXI CCAn_nnnn'nTGG Cuts at: 2945 2945 Size: 7024 BstYI r'GATC_y Cuts at: 1972 2237 3665 3870 4041 4354 4721 5106 5123 Size: 265 1428 205 171 313 367 385 17 Cuts at: 5123 6575 6661 6673 7019 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1428 385 367 346 313 265 205 171 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3898 4314 513 Size: 552 939 1894 416 3223 Fragments arranged by size: 3223 1894 939 552 416 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6285 Fragments arranged by size: 6285 683 56 BtsI GCAGTG_nn' Cuts at: 2601 3112 4540 5334 5354 2601 Size: 511 1428 794 20 4271 Fragments arranged by size: 4271 1428 794 511 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7024 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2869 2904 529 Size: 35 2305 35 4649 Fragments arranged by size: 4649 2305 35 35 DraI TTT'AAA Cuts at: 4328 4328 Size: 7024 DraIII CAC_nnn'GTG Cuts at: 3988 3988 Size: 7024 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3824 6031 809 Size: 507 2508 2207 1802 Fragments arranged by size: 2508 2207 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2281 2890 4099 Size: 54 1425 50 275 18 451 609 1209 Cuts at: 4099 4471 5360 6989 8 Size: 372 889 1629 43 Fragments arranged by size: 1629 1425 1209 889 609 451 372 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4471 1537 Size: 275 2659 4090 Fragments arranged by size: 4090 2659 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2229 2694 3756 3947 4217 4954 1353 Size: 876 465 1062 191 270 737 3423 Fragments arranged by size: 3423 1062 876 737 465 270 191 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2456 2823 3446 3572 4273 5611 Size: 1064 138 867 367 623 126 701 1338 Cuts at: 5611 5985 6131 387 Size: 374 146 1280 Fragments arranged by size: 1338 1280 1064 867 701 623 374 367 146 138 126 EcoICRI GAG'CTC Cuts at: 2075 2075 Size: 7024 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2967 3188 3356 3780 4031 5087 5684 Size: 393 1335 221 168 424 251 1056 597 Cuts at: 5684 6471 6739 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1335 1056 787 597 424 393 268 251 221 168 EcoNI CCTnn'n_nnAGG Cuts at: 2992 3266 2992 Size: 274 6750 EcoO109I rG'GnC_Cy Cuts at: 2197 3212 3213 3258 3259 2197 Size: 1015 1 45 1 5962 Fragments arranged by size: 5962 1015 45 1 1 EcoRI G'AATT_C Cuts at: 4333 4711 4333 Size: 378 6646 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2783 3642 2783 Size: 859 6165 FspI TGC'GCA Cuts at: 4091 5530 4091 Size: 1439 5585 HaeII r_GCGC'y Cuts at: 1678 2093 6171 1678 Size: 415 4078 2531 Fragments arranged by size: 4078 2531 415 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2144 2162 2176 2194 2810 Size: 32 135 32 572 18 14 18 616 Cuts at: 2810 2842 5665 5697 5739 5771 1373 Size: 32 2823 32 42 32 2626 Fragments arranged by size: 2823 2626 616 572 135 42 32 32 32 32 32 18 18 14 HincII GTy'rAC Cuts at: 669 1657 1938 4368 4614 669 Size: 988 281 2430 246 3079 Fragments arranged by size: 3079 2430 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7024 HpaI GTT'AAC Cuts at: 4614 4614 Size: 7024 KpnI G_GTAC'C Cuts at: 4349 4349 Size: 7024 MfeI C'AATT_G Cuts at: 4623 4623 Size: 7024 MluI A'CGCG_T Cuts at: 6900 6900 Size: 7024 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3997 6138 6322 1305 Size: 2692 2141 184 2007 Fragments arranged by size: 2692 2141 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2283 10 Size: 54 1425 343 451 4751 Fragments arranged by size: 4751 1425 451 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4062 4982 5341 5500 518 Size: 648 2896 920 359 159 2042 Fragments arranged by size: 2896 2042 920 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2911 4051 4440 5117 6265 6510 1606 Size: 1305 1140 389 677 1148 245 2120 Fragments arranged by size: 2120 1305 1148 1140 677 389 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3898 513 Size: 552 939 1894 3639 Fragments arranged by size: 3639 1894 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7024 NheI G'CTAG_C Cuts at: 1052 4228 1052 Size: 3176 3848 NotI GC'GGCC_GC Cuts at: 4471 4471 Size: 7024 NspI r_CATG'y Cuts at: 1132 1229 2486 3565 4067 4096 4382 5927 1132 Size: 97 1257 1079 502 29 286 1545 2229 Fragments arranged by size: 2229 1545 1257 1079 502 286 97 29 PasI CC'CwG_GG Cuts at: 1348 3254 3262 1348 Size: 1906 8 5110 Fragments arranged by size: 5110 1906 8 PciI A'CATG_T Cuts at: 4063 5923 4063 Size: 1860 5164 PflMI CCAn_nnn'nTGG Cuts at: 1108 3904 1108 Size: 2796 4228 PmeI GTTT'AAAC Cuts at: 4328 4328 Size: 7024 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5093 5125 6606 6638 5093 Size: 32 1481 32 5479 Fragments arranged by size: 5479 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2197 2197 Size: 7024 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7024 PsiI TTA'TAA Cuts at: 3069 4594 3069 Size: 1525 5499 PspOMI G'GGCC_C Cuts at: 2042 3212 3258 2042 Size: 1170 46 5808 Fragments arranged by size: 5808 1170 46 PspXI vC'TCGA_Gb Cuts at: 3788 3788 Size: 7024 PstI C_TGCA'G Cuts at: 830 2390 3163 4161 4376 830 Size: 1560 773 998 215 3478 Fragments arranged by size: 3478 1560 998 773 215 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7024 PvuII CAG'CTG Cuts at: 4051 4051 Size: 7024 RsrII CG'GwC_CG Cuts at: 2317 2317 Size: 7024 SacI G_AGCT'C Cuts at: 2077 2077 Size: 7024 SalI G'TCGA_C Cuts at: 1936 4366 1936 Size: 2430 4594 SbfI CC_TGCA'GG Cuts at: 4376 4376 Size: 7024 ScaI AGT'ACT Cuts at: 5272 6984 5272 Size: 1712 5312 SexAI A'CCwGG_T Cuts at: 2987 2987 Size: 7024 SfcI C'TryA_G Cuts at: 826 1045 2275 2386 2901 3159 4157 4372 5507 Size: 219 1230 111 515 258 998 215 1135 Cuts at: 5507 6188 6379 826 Size: 681 191 1471 Fragments arranged by size: 1471 1230 1135 998 681 515 258 219 215 191 111 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7024 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7024 SmaI CCC'GGG Cuts at: 1872 3210 4351 1872 Size: 1338 1141 4545 Fragments arranged by size: 4545 1338 1141 SmlI C'TyrA_G Cuts at: 820 1017 1943 2339 2348 3314 3575 3788 5128 Size: 197 926 396 9 966 261 213 1340 Cuts at: 5128 6029 6291 820 Size: 901 262 1553 Fragments arranged by size: 1553 1340 966 926 901 396 262 261 213 197 9 SnaBI TAC'GTA Cuts at: 493 4815 493 Size: 4322 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7024 SphI G_CATG'C Cuts at: 1132 2486 4382 1132 Size: 1354 1896 3774 Fragments arranged by size: 3774 1896 1354 SspI AAT'ATT Cuts at: 5 52 4948 5 Size: 47 4896 2081 Fragments arranged by size: 4896 2081 47 StuI AGG'CCT Cuts at: 1517 2705 2766 1517 Size: 1188 61 5775 Fragments arranged by size: 5775 1188 61 StyI C'CwwG_G Cuts at: 513 1065 2004 3898 4463 513 Size: 552 939 1894 565 3074 Fragments arranged by size: 3074 1894 939 565 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3188 3511 3896 4074 5051 5068 5221 Size: 1255 1608 323 385 178 977 17 153 Cuts at: 5221 5406 5851 325 Size: 185 445 1498 Fragments arranged by size: 1608 1498 1255 977 445 385 323 185 178 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2412 3558 5270 Size: 274 80 33 51 1443 435 1146 1712 Cuts at: 5270 6982 96 Size: 1712 138 Fragments arranged by size: 1712 1712 1443 1146 435 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2043 2075 2301 2333 2494 2526 2940 2972 3711 Size: 32 226 32 161 32 414 32 739 Cuts at: 3711 3743 4026 4058 6984 7016 2043 Size: 32 283 32 2926 32 2051 Fragments arranged by size: 2926 2051 739 414 283 226 161 32 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7024 XbaI T'CTAG_A Cuts at: 4360 4360 Size: 7024 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2828 2828 Size: 7024 XhoI C'TCGA_G Cuts at: 1943 3314 3788 1943 Size: 1371 474 5179 Fragments arranged by size: 5179 1371 474 XmaI C'CCGG_G Cuts at: 1870 3208 4349 1870 Size: 1338 1141 4545 Fragments arranged by size: 4545 1338 1141 XmnI GAAnn'nnTTC Cuts at: 3374 3933 5153 3374 Size: 559 1220 5245 Fragments arranged by size: 5245 1220 559 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4832 276 Size: 53 83 186 936 3298 2468 Fragments arranged by size: 3298 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII RsrII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PsrI SacII SanDI SapI SfiI SfoI SrfI SwaI