(Circular) (Six-base) MAPSORT of: pFN21AB8005.seq Check: 9322 from: 1 to: 6949 pFN21AB8005 6949 bp With 182 enzymes: SgfI * January 31, 2011 17:48 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4759 278 Size: 53 83 186 936 3223 2468 Fragments arranged by size: 3223 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4292 1937 Size: 2355 4594 Acc65I G'GTAC_C Cuts at: 4270 4270 Size: 6949 AclI AA'CG_TT Cuts at: 1563 5076 5449 1563 Size: 3513 373 3063 Fragments arranged by size: 3513 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2396 2658 3866 4004 5012 6396 6664 1632 Size: 764 262 1208 138 1008 1384 268 1917 Fragments arranged by size: 1917 1384 1208 1008 764 268 262 138 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6752 AflIII A'CryG_T Cuts at: 1203 2660 4229 5848 6825 1203 Size: 1457 1569 1619 977 1327 Fragments arranged by size: 1619 1569 1457 1327 977 AgeI A'CCGG_T Cuts at: 5780 5780 Size: 6949 AhdI GACnn_n'nnGTC Cuts at: 5678 5678 Size: 6949 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6949 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3196 4095 1184 Size: 2012 899 4038 Fragments arranged by size: 4038 2012 899 AlwNI CAG_nnn'CTG Cuts at: 2159 4692 6264 2159 Size: 2533 1572 2844 Fragments arranged by size: 2844 2533 1572 ApaI G_GGCC'C Cuts at: 2037 2037 Size: 6949 ApaLI G'TGCA_C Cuts at: 5006 6162 5006 Size: 1156 5793 ApoI r'AATT_y Cuts at: 1493 3135 3278 4258 4484 4636 6745 1493 Size: 1642 143 980 226 152 2109 1697 Fragments arranged by size: 2109 1697 1642 980 226 152 143 AseI AT'TA_AT Cuts at: 160 1032 3269 5503 160 Size: 872 2237 2234 1606 Fragments arranged by size: 2237 2234 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6949 AvaI C'yCGr_G Cuts at: 1870 1943 4041 4087 4274 1870 Size: 73 2098 46 187 4545 Fragments arranged by size: 4545 2098 187 73 46 BamHI G'GATC_C Cuts at: 4034 4279 4646 4034 Size: 245 367 6337 Fragments arranged by size: 6337 367 245 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4270 5725 618 Size: 325 217 627 2483 1455 1842 Fragments arranged by size: 2483 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2037 3876 4268 1391 Size: 646 1839 392 4072 Fragments arranged by size: 4072 1839 646 392 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2291 2402 2664 3854 928 Size: 971 392 111 262 1190 4023 Fragments arranged by size: 4023 1190 971 392 262 111 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5140 5174 1903 Size: 34 3203 34 3678 Fragments arranged by size: 3678 3203 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3603 3725 4841 6051 3603 Size: 122 1116 1210 4501 Fragments arranged by size: 4501 1210 1116 122 BclI T'GATC_A Cuts at: 1548 1548 Size: 6949 BfrBI ATG'CAT Cuts at: 3668 3668 Size: 6949 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5560 136 Size: 107 122 71 631 605 3888 1525 Fragments arranged by size: 3888 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3253 6944 3253 Size: 3691 3258 BlpI GC'TnA_GC Cuts at: 4366 4366 Size: 6949 Bme1580I G_kGCm'C Cuts at: 1790 2037 2859 5010 6166 1790 Size: 247 822 2151 1156 2573 Fragments arranged by size: 2573 2151 1156 822 247 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6949 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4680 5638 442 Size: 416 53 83 399 24 3263 958 1753 Fragments arranged by size: 3263 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6949 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2739 3787 5609 1239 Size: 1500 1048 1822 2579 Fragments arranged by size: 2579 1822 1500 1048 Bpu10I CC'TnA_GC Cuts at: 2115 2115 Size: 6949 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3635 5074 5939 6237 3635 Size: 1439 865 298 4347 Fragments arranged by size: 4347 1439 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2415 3108 3238 5612 882 Size: 617 916 693 130 2374 2219 Fragments arranged by size: 2374 2219 916 693 617 130 BsaAI yAC'GTr Cuts at: 493 1204 4740 493 Size: 711 3536 2702 Fragments arranged by size: 3536 2702 711 BsaBI GATnn'nnATC Cuts at: 3585 6943 3585 Size: 3358 3591 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4756 5138 5773 275 Size: 53 83 186 936 3223 382 635 1451 Fragments arranged by size: 3223 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3433 3583 5382 5780 6054 6201 6612 3433 Size: 150 1799 398 274 147 411 3770 Fragments arranged by size: 3770 1799 411 398 274 150 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2430 2460 1178 Size: 30 165 30 1027 30 5667 Fragments arranged by size: 5667 1027 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2710 2742 1186 Size: 1524 32 5393 Fragments arranged by size: 5393 1524 32 BseYI C'CCAG_C Cuts at: 2490 6152 6648 2490 Size: 3662 496 2791 Fragments arranged by size: 3662 2791 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4105 1163 Size: 573 2369 4007 Fragments arranged by size: 4007 2369 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2576 4399 5160 6188 1540 Size: 275 186 575 1823 761 1028 2301 Fragments arranged by size: 2301 1823 1028 761 575 275 186 BsiHKAI G_wGCw'C Cuts at: 2837 3876 5010 5095 6166 2837 Size: 1039 1134 85 1071 3620 Fragments arranged by size: 3620 1134 1071 1039 85 BsmI GAATG_Cn' Cuts at: 3092 4460 4553 3092 Size: 1368 93 5488 Fragments arranged by size: 5488 1368 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2037 2837 2859 3876 4268 5010 5095 Size: 399 247 800 22 1017 392 742 85 Cuts at: 5095 6166 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 1017 800 742 399 392 247 85 22 BspEI T'CCGG_A Cuts at: 3433 3583 6612 3433 Size: 150 3029 3770 Fragments arranged by size: 3770 3029 150 BspHI T'CATG_A Cuts at: 3234 4838 6568 3234 Size: 1604 1730 3615 Fragments arranged by size: 3615 1730 1604 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3164 4140 4304 844 Size: 2320 976 164 3489 Fragments arranged by size: 3489 2320 976 164 BsrBI CCG'CTC Cuts at: 1394 1823 4836 1394 Size: 429 3013 3507 Fragments arranged by size: 3507 3013 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2147 2561 2897 3178 3401 3518 4067 Size: 1183 899 414 336 281 223 117 549 Cuts at: 4067 5444 5618 65 Size: 1377 174 1396 Fragments arranged by size: 1396 1377 1183 899 549 414 336 281 223 174 117 BsrFI r'CCGG_y Cuts at: 1700 1810 2575 5593 5780 1700 Size: 110 765 3018 187 2869 Fragments arranged by size: 3018 2869 765 187 110 BsrGI T'GTAC_A Cuts at: 96 3941 96 Size: 3845 3104 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6949 BssSI C'ACGA_G Cuts at: 5009 6021 5009 Size: 1012 5937 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6949 BstBI TT'CG_AA Cuts at: 5753 5753 Size: 6949 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6949 BstXI CCAn_nnnn'nTGG Cuts at: 2490 3461 2490 Size: 971 5978 BstYI r'GATC_y Cuts at: 1972 2132 2256 2677 2863 2916 2983 3253 3580 Size: 160 124 421 186 53 67 270 327 Cuts at: 3580 3586 4034 4279 4646 5031 5048 6500 6586 Size: 6 448 245 367 385 17 1452 86 Cuts at: 6586 6598 6944 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 448 421 385 367 346 327 270 245 186 160 124 86 67 53 17 12 6 Bsu36I CC'TnA_GG Cuts at: 3168 3168 Size: 6949 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6397 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6210 Fragments arranged by size: 6210 683 56 BtsI GCAGTG_nn' Cuts at: 4465 5259 5279 4465 Size: 794 20 6135 Fragments arranged by size: 6135 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6949 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6914 DraI TTT'AAA Cuts at: 3018 4253 3018 Size: 1235 5714 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5956 809 Size: 507 4640 1802 Fragments arranged by size: 4640 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4396 5285 6914 Size: 54 1425 50 275 18 2566 889 1629 Cuts at: 6914 8 Size: 43 Fragments arranged by size: 2566 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4396 1537 Size: 275 2584 4090 Fragments arranged by size: 4090 2584 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2880 3198 3377 3993 4879 1353 Size: 1527 318 179 616 886 3423 Fragments arranged by size: 3423 1527 886 616 318 179 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5536 5910 6056 387 Size: 1064 138 3947 374 146 1280 Fragments arranged by size: 3947 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3874 3874 Size: 6949 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2396 2658 2739 3787 3866 4004 5012 Size: 393 764 262 81 1048 79 138 1008 Cuts at: 5012 5609 6396 6664 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1048 1008 787 764 597 393 268 262 138 81 79 EcoNI CCTnn'n_nnAGG Cuts at: 3171 3171 Size: 6949 EcoRI G'AATT_C Cuts at: 4258 4636 4258 Size: 378 6571 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3314 3314 Size: 6949 FspI TGC'GCA Cuts at: 5455 5455 Size: 6949 HaeII r_GCGC'y Cuts at: 1678 2566 6096 1678 Size: 888 3530 2531 Fragments arranged by size: 3530 2531 888 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2901 2933 5590 5622 5664 Size: 32 135 32 1329 32 2657 32 42 Cuts at: 5664 5696 1373 Size: 32 2626 Fragments arranged by size: 2657 2626 1329 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2953 4293 4539 669 Size: 988 281 1015 1340 246 3079 Fragments arranged by size: 3079 1340 1015 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6949 HpaI GTT'AAC Cuts at: 4539 4539 Size: 6949 KpnI G_GTAC'C Cuts at: 4274 4274 Size: 6949 MfeI C'AATT_G Cuts at: 2878 3032 3536 4548 2878 Size: 154 504 1012 5279 Fragments arranged by size: 5279 1012 504 154 MluI A'CGCG_T Cuts at: 6825 6825 Size: 6949 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2119 2328 3442 6063 6247 1305 Size: 814 209 1114 2621 184 2007 Fragments arranged by size: 2621 2007 1114 814 209 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5127 Fragments arranged by size: 5127 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3239 4907 5266 5425 518 Size: 648 2073 1668 359 159 2042 Fragments arranged by size: 2073 2042 1668 648 359 159 MspA1I CmG'CkG Cuts at: 1606 4365 5042 6190 6435 1606 Size: 2759 677 1148 245 2120 Fragments arranged by size: 2759 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6397 NdeI CA'TA_TG Cuts at: 387 3670 387 Size: 3283 3666 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6949 NotI GC'GGCC_GC Cuts at: 4396 4396 Size: 6949 NsiI A_TGCA'T Cuts at: 3670 3670 Size: 6949 NspI r_CATG'y Cuts at: 1132 1229 2664 4233 4307 5852 1132 Size: 97 1435 1569 74 1545 2229 Fragments arranged by size: 2229 1569 1545 1435 97 74 PasI CC'CwG_GG Cuts at: 1348 2859 1348 Size: 1511 5438 PciI A'CATG_T Cuts at: 2660 4229 5848 2660 Size: 1569 1619 3761 Fragments arranged by size: 3761 1619 1569 PflMI CCAn_nnn'nTGG Cuts at: 1108 2642 3324 3365 1108 Size: 1534 682 41 4692 Fragments arranged by size: 4692 1534 682 41 PfoI T'CCnGG_A Cuts at: 2905 2905 Size: 6949 PmeI GTTT'AAAC Cuts at: 4253 4253 Size: 6949 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3105 3137 5018 5050 6531 6563 3105 Size: 32 1881 32 1481 32 3491 Fragments arranged by size: 3491 1881 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2457 1613 Size: 844 6105 PsiI TTA'TAA Cuts at: 3531 4519 3531 Size: 988 5961 PspOMI G'GGCC_C Cuts at: 2033 2033 Size: 6949 PstI C_TGCA'G Cuts at: 830 2459 3427 3810 4056 4084 4301 830 Size: 1629 968 383 246 28 217 3478 Fragments arranged by size: 3478 1629 968 383 246 217 28 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6949 SacI G_AGCT'C Cuts at: 3876 3876 Size: 6949 SalI G'TCGA_C Cuts at: 1936 4291 1936 Size: 2355 4594 SapI GCTCTTCn'nnn_ Cuts at: 2880 3993 2880 Size: 1113 5836 SbfI CC_TGCA'GG Cuts at: 4301 4301 Size: 6949 ScaI AGT'ACT Cuts at: 2058 5197 6909 2058 Size: 3139 1712 2098 Fragments arranged by size: 3139 2098 1712 SexAI A'CCwGG_T Cuts at: 2073 3117 2073 Size: 1044 5905 SfcI C'TryA_G Cuts at: 826 1045 2151 2455 3423 3806 4052 4080 4297 Size: 219 1106 304 968 383 246 28 217 Cuts at: 4297 5432 6113 6304 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1106 968 681 383 304 246 219 217 191 28 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6949 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6949 SmaI CCC'GGG Cuts at: 1872 4276 1872 Size: 2404 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3614 4041 5053 5954 6216 820 Size: 197 926 1671 427 1012 901 262 1553 Fragments arranged by size: 1671 1553 1012 926 901 427 262 197 SnaBI TAC'GTA Cuts at: 493 4740 493 Size: 4247 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6949 SphI G_CATG'C Cuts at: 1132 4307 1132 Size: 3175 3774 SspI AAT'ATT Cuts at: 5 52 2588 4873 5 Size: 47 2536 2285 2081 Fragments arranged by size: 2536 2285 2081 47 StuI AGG'CCT Cuts at: 1517 3297 1517 Size: 1780 5169 StyI C'CwwG_G Cuts at: 513 1065 3101 3558 4388 513 Size: 552 2036 457 830 3074 Fragments arranged by size: 3074 2036 830 552 457 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2439 4976 4993 5146 5331 5776 325 Size: 1255 859 2537 17 153 185 445 1498 Fragments arranged by size: 2537 1498 1255 859 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2056 3941 5195 Size: 274 80 33 51 1443 79 1885 1254 Cuts at: 5195 6907 96 Size: 1712 138 Fragments arranged by size: 1885 1712 1443 1254 274 138 80 79 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2433 2465 3468 3500 4227 4259 6909 6941 2433 Size: 32 1003 32 727 32 2650 32 2441 Fragments arranged by size: 2650 2441 1003 727 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6949 XbaI T'CTAG_A Cuts at: 4285 4285 Size: 6949 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3255 3255 Size: 6949 XhoI C'TCGA_G Cuts at: 1943 4041 1943 Size: 2098 4851 XmaI C'CCGG_G Cuts at: 1870 4274 1870 Size: 2404 4545 XmnI GAAnn'nnTTC Cuts at: 3681 3828 5078 3681 Size: 147 1250 5552 Fragments arranged by size: 5552 1250 147 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4757 276 Size: 53 83 186 936 3223 2468 Fragments arranged by size: 3223 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI BstZ17I DraIII EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacII SanDI SfiI SfoI SrfI SwaI