(Circular) (Six-base) MAPSORT of: pFN21AB8004.seq Check: 9225 from: 1 to: 6817 pFN21AB8004 6817 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4627 278 Size: 53 83 186 936 3091 2468 Fragments arranged by size: 3091 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2526 2733 4160 1937 Size: 589 207 1427 4594 Fragments arranged by size: 4594 1427 589 207 Acc65I G'GTAC_C Cuts at: 2066 3426 4138 2066 Size: 1360 712 4745 Fragments arranged by size: 4745 1360 712 AclI AA'CG_TT Cuts at: 1563 4944 5317 1563 Size: 3381 373 3063 Fragments arranged by size: 3381 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2483 2505 2562 2654 3319 4880 6264 6532 Size: 851 22 57 92 665 1561 1384 268 Cuts at: 6532 1632 Size: 1917 Fragments arranged by size: 1917 1561 1384 851 665 268 92 57 22 AfeI AGC'GCT Cuts at: 3789 3789 Size: 6817 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6620 AflIII A'CryG_T Cuts at: 1203 5716 6693 1203 Size: 4513 977 1327 Fragments arranged by size: 4513 1327 977 AgeI A'CCGG_T Cuts at: 5648 5648 Size: 6817 AhdI GACnn_n'nnGTC Cuts at: 5546 5546 Size: 6817 AleI CACnn'nnGTG Cuts at: 1166 3923 1166 Size: 2757 4060 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6817 AlwNI CAG_nnn'CTG Cuts at: 2215 2766 3991 4560 6132 2215 Size: 551 1225 569 1572 2900 Fragments arranged by size: 2900 1572 1225 569 551 ApaI G_GGCC'C Cuts at: 3621 3621 Size: 6817 ApaLI G'TGCA_C Cuts at: 4874 6030 4874 Size: 1156 5661 ApoI r'AATT_y Cuts at: 1493 4126 4352 4504 6613 1493 Size: 2633 226 152 2109 1697 Fragments arranged by size: 2633 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5371 160 Size: 872 4339 1606 Fragments arranged by size: 4339 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6817 AvaI C'yCGr_G Cuts at: 1870 1943 2031 3402 4075 4142 1870 Size: 73 88 1371 673 67 4545 Fragments arranged by size: 4545 1371 673 88 73 67 BamHI G'GATC_C Cuts at: 3126 4147 4514 3126 Size: 1021 367 5429 Fragments arranged by size: 5429 1021 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2066 2425 3426 3964 4138 Size: 325 217 627 279 359 1001 538 174 Cuts at: 4138 5593 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1001 627 538 359 325 279 217 174 BanII G_rGCy'C Cuts at: 1391 3201 3621 3684 3728 3738 4136 1391 Size: 1810 420 63 44 10 398 4072 Fragments arranged by size: 4072 1810 420 398 63 44 10 BbeI G_GCGC'C Cuts at: 3968 3968 Size: 6817 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2961 3407 928 Size: 971 1062 446 4338 Fragments arranged by size: 4338 1062 971 446 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2266 2300 2633 2667 5008 5042 1903 Size: 34 329 34 333 34 2341 34 3678 Fragments arranged by size: 3678 2341 333 329 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2453 4709 5919 2453 Size: 2256 1210 3351 Fragments arranged by size: 3351 2256 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6817 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3962 3979 5428 Size: 107 122 71 631 605 2290 17 1449 Cuts at: 5428 136 Size: 1525 Fragments arranged by size: 2290 1525 1449 631 605 122 107 71 17 BglII A'GATC_T Cuts at: 3093 6812 3093 Size: 3719 3098 BlpI GC'TnA_GC Cuts at: 4234 4234 Size: 6817 Bme1580I G_kGCm'C Cuts at: 1790 2430 3621 4878 6034 1790 Size: 640 1191 1257 1156 2573 Fragments arranged by size: 2573 1257 1191 1156 640 BmgBI CAC'GTC Cuts at: 1318 3049 1318 Size: 1731 5086 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2396 2433 2539 Size: 416 53 83 399 24 979 37 106 Cuts at: 2539 3215 4548 5506 442 Size: 676 1333 958 1753 Fragments arranged by size: 1753 1333 979 958 676 416 399 106 83 53 37 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6817 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2182 2738 3038 3131 5477 1239 Size: 943 556 300 93 2346 2579 Fragments arranged by size: 2579 2346 943 556 300 93 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2281 3137 3740 4942 5807 6105 2281 Size: 856 603 1202 865 298 2993 Fragments arranged by size: 2993 1202 865 856 603 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3703 5480 882 Size: 617 2204 1777 2219 Fragments arranged by size: 2219 2204 1777 617 BsaAI yAC'GTr Cuts at: 493 1204 4608 493 Size: 711 3404 2702 Fragments arranged by size: 3404 2702 711 BsaBI GATnn'nnATC Cuts at: 2396 6811 2396 Size: 4415 2402 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3522 3965 3971 4624 Size: 53 83 186 936 1989 443 6 653 Cuts at: 4624 5006 5641 275 Size: 382 635 1451 Fragments arranged by size: 1989 1451 936 653 635 443 382 186 83 53 6 BsaWI w'CCGG_w Cuts at: 3294 3730 5250 5648 5922 6069 6480 3294 Size: 436 1520 398 274 147 411 3631 Fragments arranged by size: 3631 1520 436 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2027 2057 2975 3005 3022 Size: 30 165 30 624 30 918 30 17 Cuts at: 3022 3052 3089 3119 3297 3327 3593 3623 3725 Size: 30 37 30 178 30 266 30 102 Cuts at: 3725 3755 1178 Size: 30 4240 Fragments arranged by size: 4240 918 624 266 178 165 102 37 30 30 30 30 30 30 30 30 30 17 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2144 3188 3692 4042 1186 Size: 958 1044 504 350 3961 Fragments arranged by size: 3961 1044 958 504 350 BseYI C'CCAG_C Cuts at: 2148 2233 2429 2759 3254 3870 6020 6516 2148 Size: 85 196 330 495 616 2150 496 2449 Fragments arranged by size: 2449 2150 616 496 495 330 196 85 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3225 1163 Size: 573 1489 4755 Fragments arranged by size: 4755 1489 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3057 4267 5028 6056 1540 Size: 275 186 1056 1210 761 1028 2301 Fragments arranged by size: 2301 1210 1056 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3684 3728 3930 4878 4963 6034 3684 Size: 44 202 948 85 1071 4467 Fragments arranged by size: 4467 1071 948 202 85 44 BsmI GAATG_Cn' Cuts at: 2278 4328 4421 2278 Size: 2050 93 4674 Fragments arranged by size: 4674 2050 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3055 3055 Size: 6817 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2430 3201 3621 3684 3728 3738 3930 Size: 399 640 771 420 63 44 10 192 Cuts at: 3930 4136 4878 4963 6034 1391 Size: 206 742 85 1071 2174 Fragments arranged by size: 2174 1071 771 742 640 420 399 206 192 85 63 44 10 BspEI T'CCGG_A Cuts at: 6480 6480 Size: 6817 BspHI T'CATG_A Cuts at: 2805 4706 6436 2805 Size: 1901 1730 3186 Fragments arranged by size: 3186 1901 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4172 844 Size: 3328 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2619 4704 1394 Size: 429 796 2085 3507 Fragments arranged by size: 3507 2085 796 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2377 5312 5486 65 Size: 1183 1129 2935 174 1396 Fragments arranged by size: 2935 1396 1183 1129 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4005 5461 5648 1700 Size: 110 2195 1456 187 2869 Fragments arranged by size: 2869 2195 1456 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6817 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6817 BssSI C'ACGA_G Cuts at: 4877 5889 4877 Size: 1012 5805 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6817 BstBI TT'CG_AA Cuts at: 5621 5621 Size: 6817 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6817 BstXI CCAn_nnnn'nTGG Cuts at: 4031 4031 Size: 6817 BstYI r'GATC_y Cuts at: 1972 2741 3093 3126 4147 4514 4899 4916 6368 Size: 769 352 33 1021 367 385 17 1452 Cuts at: 6368 6454 6466 6812 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1021 769 385 367 352 346 86 33 17 12 Bsu36I CC'TnA_GG Cuts at: 3189 3189 Size: 6817 BtgI C'CryG_G Cuts at: 513 1065 2004 2690 3072 3613 3693 513 Size: 552 939 686 382 541 80 3637 Fragments arranged by size: 3637 939 686 552 541 382 80 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6078 Fragments arranged by size: 6078 683 56 BtsI GCAGTG_nn' Cuts at: 2282 3868 4333 5127 5147 2282 Size: 1586 465 794 20 3952 Fragments arranged by size: 3952 1586 794 465 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6817 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2286 2321 529 Size: 35 1722 35 5025 Fragments arranged by size: 5025 1722 35 35 DraI TTT'AAA Cuts at: 2872 4121 2872 Size: 1249 5568 DraIII CAC_nnn'GTG Cuts at: 2248 3644 2248 Size: 1396 5421 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5824 809 Size: 507 4508 1802 Fragments arranged by size: 4508 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 3054 4264 Size: 54 1425 50 275 18 177 1047 1210 Cuts at: 4264 5153 6782 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1210 1047 889 275 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3054 4264 1537 Size: 275 1242 1210 4090 Fragments arranged by size: 4090 1242 1210 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2349 3935 4747 1353 Size: 996 1586 812 3423 Fragments arranged by size: 3423 1586 996 812 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2096 3554 5404 5778 5924 387 Size: 1064 138 507 1458 1850 374 146 1280 Fragments arranged by size: 1850 1458 1280 1064 507 374 146 138 EcoICRI GAG'CTC Cuts at: 3682 3726 3682 Size: 44 6773 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2182 2483 2505 2562 2654 2738 3038 Size: 393 550 301 22 57 92 84 300 Cuts at: 3038 3131 3319 4880 5477 6264 6532 1239 Size: 93 188 1561 597 787 268 1524 Fragments arranged by size: 1561 1524 787 597 550 393 301 300 268 188 93 92 84 57 22 EcoNI CCTnn'n_nnAGG Cuts at: 2114 2126 3388 3455 2114 Size: 12 1262 67 5476 Fragments arranged by size: 5476 1262 67 12 EcoO109I rG'GnC_Cy Cuts at: 2119 2533 2573 3184 3502 3618 4091 2119 Size: 414 40 611 318 116 473 4845 Fragments arranged by size: 4845 611 473 414 318 116 40 EcoRI G'AATT_C Cuts at: 4126 4504 4126 Size: 378 6439 FspI TGC'GCA Cuts at: 5323 5323 Size: 6817 HaeII r_GCGC'y Cuts at: 1678 2863 3791 3968 5964 1678 Size: 1185 928 177 1996 2531 Fragments arranged by size: 2531 1996 1185 928 177 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2027 2059 2161 2193 2726 Size: 32 135 32 455 32 102 32 533 Cuts at: 2726 2758 3297 3329 3725 3757 4058 4090 5458 Size: 32 539 32 396 32 301 32 1368 Cuts at: 5458 5490 5532 5564 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1368 539 533 455 396 301 135 102 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4161 4407 669 Size: 988 281 2223 246 3079 Fragments arranged by size: 3079 2223 988 281 246 HindIII A'AGCT_T Cuts at: 748 2299 2636 748 Size: 1551 337 4929 Fragments arranged by size: 4929 1551 337 HpaI GTT'AAC Cuts at: 4407 4407 Size: 6817 KasI G'GCGC_C Cuts at: 3964 3964 Size: 6817 KpnI G_GTAC'C Cuts at: 2070 3430 4142 2070 Size: 1360 712 4745 Fragments arranged by size: 4745 1360 712 MfeI C'AATT_G Cuts at: 4416 4416 Size: 6817 MluI A'CGCG_T Cuts at: 6693 6693 Size: 6817 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3445 5931 6115 1305 Size: 2140 2486 184 2007 Fragments arranged by size: 2486 2140 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 10 Size: 54 1425 343 177 4818 Fragments arranged by size: 4818 1425 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 3797 3923 4775 5134 5293 518 Size: 648 2631 126 852 359 159 2042 Fragments arranged by size: 2631 2042 852 648 359 159 126 MspA1I CmG'CkG Cuts at: 1606 2709 3366 3615 4233 4910 6058 6303 1606 Size: 1103 657 249 618 677 1148 245 2120 Fragments arranged by size: 2120 1148 1103 677 657 618 249 245 NarI GG'CG_CC Cuts at: 3965 3965 Size: 6817 NcoI C'CATG_G Cuts at: 513 1065 2004 3693 513 Size: 552 939 1689 3637 Fragments arranged by size: 3637 1689 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6817 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6817 NotI GC'GGCC_GC Cuts at: 4264 4264 Size: 6817 NspI r_CATG'y Cuts at: 1132 1229 2144 3494 4175 5720 1132 Size: 97 915 1350 681 1545 2229 Fragments arranged by size: 2229 1545 1350 915 681 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6817 PciI A'CATG_T Cuts at: 5716 5716 Size: 6817 PflMI CCAn_nnn'nTGG Cuts at: 1108 3644 1108 Size: 2536 4281 PfoI T'CCnGG_A Cuts at: 2844 2906 2844 Size: 62 6755 PmeI GTTT'AAAC Cuts at: 4121 4121 Size: 6817 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4886 4918 6399 6431 4886 Size: 32 1481 32 5272 Fragments arranged by size: 5272 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2119 2533 4091 2119 Size: 414 1558 4845 Fragments arranged by size: 4845 1558 414 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6817 PsiI TTA'TAA Cuts at: 4387 4387 Size: 6817 PspOMI G'GGCC_C Cuts at: 3617 3617 Size: 6817 PstI C_TGCA'G Cuts at: 830 3089 3474 3987 4169 830 Size: 2259 385 513 182 3478 Fragments arranged by size: 3478 2259 513 385 182 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6817 PvuII CAG'CTG Cuts at: 3366 3366 Size: 6817 SacI G_AGCT'C Cuts at: 3684 3728 3684 Size: 44 6773 SacII CC_GC'GG Cuts at: 3616 3616 Size: 6817 SalI G'TCGA_C Cuts at: 1936 4159 1936 Size: 2223 4594 SapI GCTCTTCn'nnn_ Cuts at: 2349 3935 2349 Size: 1586 5231 SbfI CC_TGCA'GG Cuts at: 3474 4169 3474 Size: 695 6122 ScaI AGT'ACT Cuts at: 3572 5065 6777 3572 Size: 1493 1712 3612 Fragments arranged by size: 3612 1712 1493 SfcI C'TryA_G Cuts at: 826 1045 3085 3361 3470 3935 3983 4165 5300 Size: 219 2040 276 109 465 48 182 1135 Cuts at: 5300 5981 6172 826 Size: 681 191 1471 Fragments arranged by size: 2040 1471 1135 681 465 276 219 191 182 109 48 SfoI GGC'GCC Cuts at: 3966 3966 Size: 6817 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6817 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6817 SmaI CCC'GGG Cuts at: 1872 2033 3404 4144 1872 Size: 161 1371 740 4545 Fragments arranged by size: 4545 1371 740 161 SmlI C'TyrA_G Cuts at: 820 1017 1943 2296 3152 3719 4921 5822 6084 Size: 197 926 353 856 567 1202 901 262 Cuts at: 6084 820 Size: 1553 Fragments arranged by size: 1553 1202 926 901 856 567 353 262 197 SnaBI TAC'GTA Cuts at: 493 4608 493 Size: 4115 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6817 SphI G_CATG'C Cuts at: 1132 2144 4175 1132 Size: 1012 2031 3774 Fragments arranged by size: 3774 2031 1012 SspI AAT'ATT Cuts at: 5 52 4741 5 Size: 47 4689 2081 Fragments arranged by size: 4689 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6817 StyI C'CwwG_G Cuts at: 513 1065 2004 2994 3063 3693 4062 4256 513 Size: 552 939 990 69 630 369 194 3074 Fragments arranged by size: 3074 990 939 630 552 369 194 69 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2425 4844 4861 5014 5199 5644 325 Size: 1255 845 2419 17 153 185 445 1498 Fragments arranged by size: 2419 1498 1255 845 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3210 3570 5063 Size: 274 80 33 51 1443 1233 360 1493 Cuts at: 5063 6775 96 Size: 1712 138 Fragments arranged by size: 1712 1493 1443 1233 360 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2975 3007 3022 3054 6777 6809 2975 Size: 32 15 32 3723 32 2983 Fragments arranged by size: 3723 2983 32 32 32 15 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6817 XbaI T'CTAG_A Cuts at: 4153 4153 Size: 6817 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3070 3256 3515 3070 Size: 186 259 6372 Fragments arranged by size: 6372 259 186 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6817 XmaI C'CCGG_G Cuts at: 1870 2031 3402 4142 1870 Size: 161 1371 740 4545 Fragments arranged by size: 4545 1371 740 161 XmnI GAAnn'nnTTC Cuts at: 4946 4946 Size: 6817 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4625 276 Size: 53 83 186 936 3091 2468 Fragments arranged by size: 3091 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SanDI SexAI SfiI SrfI SwaI