Restriction map for
pFN21AB6008
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# NO CUT ENZYMES ( 44 enzymes) #
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AflII AgeI AscI AseI AsnI
AvrII BclI BfrI BlnI BsaBI
BsiWI BssHII BssSI Bst1107I BstBI
BstZ17I CpoI DraI EcoRI FbaI
FseI HpaI MamI MfeI MluI
MunI NdeI NheI NotI NruI
NspV PacI PinAI PmeI PshBI
RsrII SalI SfiI SfuI SgrAI
SnaBI SpeI SspI SwaI
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# ONE CUT ENZYMES ( 29 enzymes) #
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AclI 6121 AACGTT AA^CGTT
Aor51HI 1064 AGCGCT AGC^GCT
Asp700I 5130 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 3920 TGCGCA TGC^GCA
BamHI 2717 GGATCC G^GATCC
BciVI 163 GGATAC GTATCC(6/5)
Bsp1407I 149 TGTACA T^GTACA
BspLU11I 8152 ACATGT A^CATGT
BsrGI 149 TGTACA T^GTACA
DrdI 316 GACCAAAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1851 CGGCCG C^GGCCG
EclXI 1851 CGGCCG C^GGCCG
Eco47III 1064 AGCGCT AGC^GCT
Eco52I 1851 CGGCCG C^GGCCG
EcoRV 8236 GATATC GAT^ATC
FspI 3920 TGCGCA TGC^GCA
HindIII 2235 AAGCTT A^AGCTT
NaeI 3608 GCCGGC GCC^GGC
NgoMIV 3608 GCCGGC G^CCGGC
PshAI 7423 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 6121 AACGTT AA^CGTT
PvuI 2606 CGATCG CGAT^CG
SapI 684 GAAGAGC GCTCTTC(1/4)
SbfI 7082 CCTGCAGG CCTGCA^GG
SphI 7745 GCATGC GCATG^C
Sse8387I 7082 CCTGCAGG CCTGCA^GG
SspBI 149 TGTACA T^GTACA
XbaI 357 TCTAGA T^CTAGA
XmnI 5130 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 37 enzymes) #
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Acc65I 298 GGTACC G^GTACC
4748 GGTACC
AccI 4810 GTCTAC GT^[AC][GT]AC
6622 GTCTAC
AccIII 1057 TCCGGA T^CCGGA
5011 TCCGGA
AhdI 6240 GACGTATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
7525 GACTCCCAGTC
AocI 994 CCTGAGG CC^T[ACGT]AGG
3620 CCTCAGG
Asp718I 298 GGTACC G^GTACC
4748 GGTACC
AspEI 6240 GACGTATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
7525 GACTCCCAGTC
BbeI 1870 GGCGCC GGCGC^C
4797 GGCGCC
BfaI 358 CTAG C^TAG
2722 CTAG
BglII 2141 AGATCT A^GATCT
6836 AGATCT
BsaI 5812 GAGACC GGTCTC(1/5)
6084 GGTCTC
BseAI 1057 TCCGGA T^CCGGA
5011 TCCGGA
BspDI 418 ATCGAT AT^CGAT
7789 ATCGAT
BspEI 1057 TCCGGA T^CCGGA
5011 TCCGGA
BspHI 3149 TCATGA T^CATGA
7973 TCATGA
BsrFI 2876 GCCGGT [GA]^CCGG[CT]
3608 GCCGGC
Bsu36I 994 CCTGAGG CC^T[ACGT]AGG
3620 CCTCAGG
Cfr10I 2876 GCCGGT [GA]^CCGG[CT]
3608 GCCGGC
ClaI 418 ATCGAT AT^CGAT
7789 ATCGAT
Eam1105I 6240 GACGTATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
7525 GACTCCCAGTC
Eco81I 994 CCTGAGG CC^T[ACGT]AGG
3620 CCTCAGG
EcoNI 2781 CCTGGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4947 CCTCATCCAGG
EcoT22I 5353 ATGCAT ATGCA^T
6139 ATGCAT
KasI 1870 GGCGCC G^GCGCC
4797 GGCGCC
KpnI 298 GGTACC GGTAC^C
4748 GGTACC
MaeI 358 CTAG C^TAG
2722 CTAG
MroI 1057 TCCGGA T^CCGGA
5011 TCCGGA
MseI 494 TTAA T^TAA
5801 TTAA
NarI 1870 GGCGCC GG^CGCC
4797 GGCGCC
NsiI 5353 ATGCAT ATGCA^T
6139 ATGCAT
PaeR7I 1091 CTCGAG C^TCGAG
2356 CTCGAG
RcaI 3149 TCATGA T^CATGA
7973 TCATGA
SacI 3081 GAGCTC GAGCT^C
6672 GAGCTC
ScaI 4622 AGTACT AGT^ACT
8048 AGTACT
SfoI 1870 GGCGCC GGC^GCC
4797 GGCGCC
Tru9I 494 TTAA T^TAA
5801 TTAA
XhoI 1091 CTCGAG C^TCGAG
2356 CTCGAG
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# 3 OR MORE CUT ENZYMES ( 148 enzymes) #
#########################################
AatII AccII AciI AcsI AcyI
AfaI AflIII AluI Alw44I AlwI
AlwNI ApaI ApaLI ApoI AspHI
AspI AvaI AvaII BalI BanI
BanII BbrPI BbsI BbvCI BbvI
BcgI BcnI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaHI
BsaJI BsaWI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrBI BsrDI BsrI BssKI BstAPI
BstEII BstF5I BstNI BstPI BstUI
BstXI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I KspI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflFI PflMI PleI PmaCI PmlI
PpuMI PstI PvuII RsaI SacII
Sau3AI Sau96I ScrFI SexAI SfaNI
SfcI SmaI SmlI StuI StyI
TaqI TfiI TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XcmI
XhoII XmaCI XmaI