(Circular) (Six-base) MAPSORT of: pFN21AB5637.seq Check: 8371 from: 1 to: 7936 pFN21AB5637 7936 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2815 3217 2815 Size: 402 7534 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2212 5746 278 Size: 53 83 186 936 676 3534 2468 Fragments arranged by size: 3534 2468 936 676 186 83 53 AccI GT'mk_AC Cuts at: 1937 5279 1937 Size: 3342 4594 Acc65I G'GTAC_C Cuts at: 3091 5257 3091 Size: 2166 5770 AclI AA'CG_TT Cuts at: 1563 6063 6436 1563 Size: 4500 373 3063 Fragments arranged by size: 4500 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2859 3405 3498 4665 4981 5999 7383 7651 Size: 1227 546 93 1167 316 1018 1384 268 Cuts at: 7651 1632 Size: 1917 Fragments arranged by size: 1917 1384 1227 1167 1018 546 316 268 93 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7739 AflIII A'CryG_T Cuts at: 1203 2465 6835 7812 1203 Size: 1262 4370 977 1327 Fragments arranged by size: 4370 1327 1262 977 AgeI A'CCGG_T Cuts at: 6767 6767 Size: 7936 AhdI GACnn_n'nnGTC Cuts at: 4372 6665 4372 Size: 2293 5643 AleI CACnn'nnGTG Cuts at: 1166 2987 1166 Size: 1821 6115 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7936 AlwNI CAG_nnn'CTG Cuts at: 2807 3239 5071 5679 7251 2807 Size: 432 1832 608 1572 3492 Fragments arranged by size: 3492 1832 1572 608 432 ApaI G_GGCC'C Cuts at: 2276 2276 Size: 7936 ApaLI G'TGCA_C Cuts at: 5993 7149 5993 Size: 1156 6780 ApoI r'AATT_y Cuts at: 1493 4160 4187 4489 4645 4949 5021 5088 5245 Size: 2667 27 302 156 304 72 67 157 Cuts at: 5245 5471 5623 7732 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2667 2109 1697 304 302 226 157 156 152 72 67 27 AseI AT'TA_AT Cuts at: 160 1032 3779 3792 5048 6490 160 Size: 872 2747 13 1256 1442 1606 Fragments arranged by size: 2747 1606 1442 1256 872 13 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7936 AvaI C'yCGr_G Cuts at: 1870 1943 2050 3894 5261 1870 Size: 73 107 1844 1367 4545 Fragments arranged by size: 4545 1844 1367 107 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3083 3116 3083 Size: 33 7903 BamHI G'GATC_C Cuts at: 3418 4742 5266 5633 3418 Size: 1324 524 367 5721 Fragments arranged by size: 5721 1324 524 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3091 3705 5257 6712 618 Size: 325 217 627 1304 614 1552 1455 1842 Fragments arranged by size: 1842 1552 1455 1304 627 614 325 217 BanII G_rGCy'C Cuts at: 1391 2061 2276 3459 3666 5217 5255 1391 Size: 670 215 1183 207 1551 38 4072 Fragments arranged by size: 4072 1551 1183 670 215 207 38 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3278 3565 4313 4458 928 Size: 971 1379 287 748 145 4406 Fragments arranged by size: 4406 1379 971 748 287 145 BbvCI CC'TCA_GC Cuts at: 3051 3051 Size: 7936 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6127 6161 1903 Size: 34 4190 34 3678 Fragments arranged by size: 4190 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2250 2824 5828 7038 2250 Size: 574 3004 1210 3148 Fragments arranged by size: 3148 3004 1210 574 BclI T'GATC_A Cuts at: 1548 4060 1548 Size: 2512 5424 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3500 6547 136 Size: 107 122 71 631 605 1828 3047 1525 Fragments arranged by size: 3047 1828 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7931 7931 Size: 7936 BlpI GC'TnA_GC Cuts at: 5353 5353 Size: 7936 Bme1580I G_kGCm'C Cuts at: 1790 2276 5997 7153 1790 Size: 486 3721 1156 2573 Fragments arranged by size: 3721 2573 1156 486 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7936 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5667 6625 442 Size: 416 53 83 399 24 4250 958 1753 Fragments arranged by size: 4250 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7936 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2232 2270 2370 2415 3243 3938 4909 6596 Size: 993 38 100 45 828 695 971 1687 Cuts at: 6596 1239 Size: 2579 Fragments arranged by size: 2579 1687 993 971 828 695 100 45 38 Bpu10I CC'TnA_GC Cuts at: 2060 2559 2577 2595 2613 2625 2631 2691 2697 Size: 499 18 18 18 12 6 60 6 Cuts at: 2697 2709 2727 3051 3217 2060 Size: 12 18 324 166 6779 Fragments arranged by size: 6779 499 324 166 60 18 18 18 18 12 12 6 6 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2104 6061 6926 7224 2104 Size: 3957 865 298 2816 Fragments arranged by size: 3957 2816 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2284 5009 6599 882 Size: 617 785 2725 1590 2219 Fragments arranged by size: 2725 2219 1590 785 617 BsaAI yAC'GTr Cuts at: 493 1204 2468 4869 5727 493 Size: 711 1264 2401 858 2702 Fragments arranged by size: 2702 2401 1264 858 711 BsaBI GATnn'nnATC Cuts at: 7930 7930 Size: 7936 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2209 5743 6125 6760 Size: 53 83 186 936 676 3534 382 635 Cuts at: 6760 275 Size: 1451 Fragments arranged by size: 3534 1451 936 676 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6369 6767 7041 7188 7599 6369 Size: 398 274 147 411 6706 Fragments arranged by size: 6706 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2487 2517 2956 2986 3010 Size: 30 165 30 1084 30 439 30 24 Cuts at: 3010 3040 3977 4007 1178 Size: 30 937 30 5107 Fragments arranged by size: 5107 1084 937 439 165 30 30 30 30 30 30 24 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2142 2172 2235 2298 2319 2322 2420 3425 Size: 956 30 63 63 21 3 98 1005 Cuts at: 3425 3428 3467 3482 4775 4912 5150 1186 Size: 3 39 15 1293 137 238 3972 Fragments arranged by size: 3972 1293 1005 956 238 137 98 63 63 39 30 21 15 3 3 BseYI C'CCAG_C Cuts at: 2800 3003 4731 7139 7635 2800 Size: 203 1728 2408 496 3101 Fragments arranged by size: 3101 2408 1728 496 203 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 7363 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2198 5386 6147 7175 1540 Size: 275 186 197 3188 761 1028 2301 Fragments arranged by size: 3188 2301 1028 761 275 197 186 BsiHKAI G_wGCw'C Cuts at: 2061 4821 5997 6082 7153 2061 Size: 2760 1176 85 1071 2844 Fragments arranged by size: 2844 2760 1176 1071 85 BsmI GAATG_Cn' Cuts at: 2364 5447 5540 2364 Size: 3083 93 4760 Fragments arranged by size: 4760 3083 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3398 3825 5215 3398 Size: 427 1390 6119 Fragments arranged by size: 6119 1390 427 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2061 2276 3459 3666 4821 5217 5255 Size: 399 271 215 1183 207 1155 396 38 Cuts at: 5255 5997 6082 7153 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1183 1155 1071 742 399 396 271 215 207 85 38 BspEI T'CCGG_A Cuts at: 7599 7599 Size: 7936 BspHI T'CATG_A Cuts at: 5825 7555 5825 Size: 1730 6206 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2815 3079 3217 5291 844 Size: 1971 264 138 2074 3489 Fragments arranged by size: 3489 2074 1971 264 138 BsrBI CCG'CTC Cuts at: 1394 1823 3568 5823 1394 Size: 429 1745 2255 3507 Fragments arranged by size: 3507 2255 1745 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6431 6605 65 Size: 1183 5183 174 1396 Fragments arranged by size: 5183 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6580 6767 1700 Size: 110 4770 187 2869 Fragments arranged by size: 4770 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7936 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7936 BssSI C'ACGA_G Cuts at: 5218 5996 7008 5218 Size: 778 1012 6146 Fragments arranged by size: 6146 1012 778 BstAPI GCAn_nnn'nTGC Cuts at: 794 3071 794 Size: 2277 5659 BstBI TT'CG_AA Cuts at: 4304 6740 4304 Size: 2436 5500 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7936 BstYI r'GATC_y Cuts at: 1972 3418 4742 4900 5036 5266 5633 6018 6035 Size: 1446 1324 158 136 230 367 385 17 Cuts at: 6035 7487 7573 7585 7931 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1446 1324 385 367 346 230 158 136 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2192 3539 3870 513 Size: 552 939 188 1347 331 4579 Fragments arranged by size: 4579 1347 939 552 331 188 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7197 Fragments arranged by size: 7197 683 56 BtsI GCAGTG_nn' Cuts at: 2124 5452 6246 6266 2124 Size: 3328 794 20 3794 Fragments arranged by size: 3794 3328 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7936 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7901 DraI TTT'AAA Cuts at: 5240 5240 Size: 7936 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3724 6943 809 Size: 507 2408 3219 1802 Fragments arranged by size: 3219 2408 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2195 4272 5383 Size: 54 1425 50 275 18 365 2077 1111 Cuts at: 5383 6272 7901 8 Size: 889 1629 43 Fragments arranged by size: 2077 1629 1425 1111 889 365 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2195 5383 1537 Size: 275 383 3188 4090 Fragments arranged by size: 4090 3188 383 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3345 3381 3423 3459 3821 4662 5866 1353 Size: 1992 36 42 36 362 841 1204 3423 Fragments arranged by size: 3423 1992 1204 841 362 42 36 36 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 6523 6897 7043 387 Size: 1064 138 435 4499 374 146 1280 Fragments arranged by size: 4499 1280 1064 435 374 146 138 EcoICRI GAG'CTC Cuts at: 2059 2059 Size: 7936 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2232 2270 2370 2415 2859 3243 3405 Size: 393 600 38 100 45 444 384 162 Cuts at: 3405 3498 3938 4665 4909 4981 5999 6596 7383 Size: 93 440 727 244 72 1018 597 787 Cuts at: 7383 7651 1239 Size: 268 1524 Fragments arranged by size: 1524 1018 787 727 600 597 444 440 393 384 268 244 162 100 93 72 45 38 EcoNI CCTnn'n_nnAGG Cuts at: 2247 2250 2247 Size: 3 7933 EcoO109I rG'GnC_Cy Cuts at: 2177 2273 4710 2177 Size: 96 2437 5403 Fragments arranged by size: 5403 2437 96 EcoRI G'AATT_C Cuts at: 5245 5623 5245 Size: 378 7558 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4194 4790 4194 Size: 596 7340 FspI TGC'GCA Cuts at: 2883 6442 2883 Size: 3559 4377 HaeII r_GCGC'y Cuts at: 1678 7083 1678 Size: 5405 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2134 2166 3010 3042 3836 Size: 32 135 32 562 32 844 32 794 Cuts at: 3836 3868 4358 4390 4424 4456 6577 6609 6651 Size: 32 490 32 34 32 2121 32 42 Cuts at: 6651 6683 1373 Size: 32 2626 Fragments arranged by size: 2626 2121 844 794 562 490 135 42 34 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3646 5280 5526 669 Size: 988 281 1708 1634 246 3079 Fragments arranged by size: 3079 1708 1634 988 281 246 HindIII A'AGCT_T Cuts at: 748 4772 748 Size: 4024 3912 HpaI GTT'AAC Cuts at: 5526 5526 Size: 7936 KpnI G_GTAC'C Cuts at: 3095 5261 3095 Size: 2166 5770 MfeI C'AATT_G Cuts at: 4227 5535 4227 Size: 1308 6628 MluI A'CGCG_T Cuts at: 7812 7812 Size: 7936 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2752 3740 7050 7234 1305 Size: 1447 988 3310 184 2007 Fragments arranged by size: 3310 2007 1447 988 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 6114 Fragments arranged by size: 6114 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2987 4867 5894 6253 6412 518 Size: 648 1821 1880 1027 359 159 2042 Fragments arranged by size: 2042 1880 1821 1027 648 359 159 MspA1I CmG'CkG Cuts at: 1606 4277 4731 5352 6029 7177 7422 1606 Size: 2671 454 621 677 1148 245 2120 Fragments arranged by size: 2671 2120 1148 677 621 454 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3539 3870 513 Size: 552 939 1535 331 4579 Fragments arranged by size: 4579 1535 939 552 331 NdeI CA'TA_TG Cuts at: 387 5161 387 Size: 4774 3162 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7936 NotI GC'GGCC_GC Cuts at: 5383 5383 Size: 7936 NspI r_CATG'y Cuts at: 1132 1229 3124 3949 4205 5294 6839 1132 Size: 97 1895 825 256 1089 1545 2229 Fragments arranged by size: 2229 1895 1545 1089 825 256 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7936 PciI A'CATG_T Cuts at: 6835 6835 Size: 7936 PflMI CCAn_nnn'nTGG Cuts at: 1108 2162 1108 Size: 1054 6882 PfoI T'CCnGG_A Cuts at: 2248 2248 Size: 7936 PmeI GTTT'AAAC Cuts at: 5240 5240 Size: 7936 PmlI CAC'GTG Cuts at: 2468 4869 2468 Size: 2401 5535 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6005 6037 7518 7550 6005 Size: 32 1481 32 6391 Fragments arranged by size: 6391 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4710 4710 Size: 7936 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7936 PsiI TTA'TAA Cuts at: 2454 3285 3582 5027 5506 2454 Size: 831 297 1445 479 4884 Fragments arranged by size: 4884 1445 831 479 297 PspOMI G'GGCC_C Cuts at: 2272 2272 Size: 7936 PstI C_TGCA'G Cuts at: 830 2812 5288 830 Size: 1982 2476 3478 Fragments arranged by size: 3478 2476 1982 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7936 SacI G_AGCT'C Cuts at: 2061 2061 Size: 7936 SalI G'TCGA_C Cuts at: 1936 5278 1936 Size: 3342 4594 SapI GCTCTTCn'nnn_ Cuts at: 3381 3423 4662 3381 Size: 42 1239 6655 Fragments arranged by size: 6655 1239 42 SbfI CC_TGCA'GG Cuts at: 5288 5288 Size: 7936 ScaI AGT'ACT Cuts at: 5118 6184 7896 5118 Size: 1066 1712 5158 Fragments arranged by size: 5158 1712 1066 SfcI C'TryA_G Cuts at: 826 1045 2808 2949 2976 5284 6419 7100 7291 Size: 219 1763 141 27 2308 1135 681 191 Cuts at: 7291 826 Size: 1471 Fragments arranged by size: 2308 1763 1471 1135 681 219 191 141 27 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7936 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7936 SmaI CCC'GGG Cuts at: 1872 2052 5263 1872 Size: 180 3211 4545 Fragments arranged by size: 4545 3211 180 SmlI C'TyrA_G Cuts at: 820 1017 1943 2083 6040 6941 7203 820 Size: 197 926 140 3957 901 262 1553 Fragments arranged by size: 3957 1553 926 901 262 197 140 SnaBI TAC'GTA Cuts at: 493 5727 493 Size: 5234 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7936 SphI G_CATG'C Cuts at: 1132 3124 4205 5294 1132 Size: 1992 1081 1089 3774 Fragments arranged by size: 3774 1992 1089 1081 SspI AAT'ATT Cuts at: 5 52 5860 5 Size: 47 5808 2081 Fragments arranged by size: 5808 2081 47 StuI AGG'CCT Cuts at: 1517 2081 1517 Size: 564 7372 StyI C'CwwG_G Cuts at: 513 1065 2004 3539 3870 4125 5375 513 Size: 552 939 1535 331 255 1250 3074 Fragments arranged by size: 3074 1535 1250 939 552 331 255 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2914 3627 3723 4260 5963 5980 6133 Size: 1255 1334 713 96 537 1703 17 153 Cuts at: 6133 6318 6763 325 Size: 185 445 1498 Fragments arranged by size: 1703 1498 1334 1255 713 537 445 185 153 96 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4467 5116 6182 Size: 274 80 33 51 1443 2490 649 1066 Cuts at: 6182 7894 96 Size: 1712 138 Fragments arranged by size: 2490 1712 1443 1066 649 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7896 7928 7896 Size: 32 7904 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7936 XbaI T'CTAG_A Cuts at: 5272 5272 Size: 7936 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3913 3913 Size: 7936 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7936 XmaI C'CCGG_G Cuts at: 1870 2050 5261 1870 Size: 180 3211 4545 Fragments arranged by size: 4545 3211 180 XmnI GAAnn'nnTTC Cuts at: 3935 6065 3935 Size: 2130 5806 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2210 5744 276 Size: 53 83 186 936 676 3534 2468 Fragments arranged by size: 3534 2468 936 676 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BbeI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI