(Circular) (Six-base) MAPSORT of: pFN21AB5593.seq Check: 7627 from: 1 to: 10294 pFN21AB5593 10294 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2534 8104 278 Size: 53 83 186 936 998 5570 2468 Fragments arranged by size: 5570 2468 998 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2538 4942 5726 7637 1937 Size: 601 2404 784 1911 4594 Fragments arranged by size: 4594 2404 1911 784 601 Acc65I G'GTAC_C Cuts at: 4318 7615 4318 Size: 3297 6997 AclI AA'CG_TT Cuts at: 1563 8421 8794 1563 Size: 6858 373 3063 Fragments arranged by size: 6858 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2586 3126 3264 4124 4257 6162 6539 6765 Size: 954 540 138 860 133 1905 377 226 Cuts at: 6765 7068 7270 8357 9741 10009 1632 Size: 303 202 1087 1384 268 1917 Fragments arranged by size: 1917 1905 1384 1087 954 860 540 377 303 268 226 202 138 133 AflII C'TTAA_G Cuts at: 820 1017 3424 820 Size: 197 2407 7690 Fragments arranged by size: 7690 2407 197 AflIII A'CryG_T Cuts at: 1203 2984 9193 10170 1203 Size: 1781 6209 977 1327 Fragments arranged by size: 6209 1781 1327 977 AgeI A'CCGG_T Cuts at: 9125 9125 Size: 10294 AhdI GACnn_n'nnGTC Cuts at: 9023 9023 Size: 10294 AleI CACnn'nnGTG Cuts at: 1166 5859 1166 Size: 4693 5601 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2608 3882 7458 1184 Size: 1424 1274 3576 4020 Fragments arranged by size: 4020 3576 1424 1274 AlwNI CAG_nnn'CTG Cuts at: 3076 6113 6833 6851 7082 7552 8037 9609 3076 Size: 3037 720 18 231 470 485 1572 3761 Fragments arranged by size: 3761 3037 1572 720 485 470 231 18 ApaI G_GGCC'C Cuts at: 7317 7317 Size: 10294 ApaLI G'TGCA_C Cuts at: 8351 9507 8351 Size: 1156 9138 ApoI r'AATT_y Cuts at: 1493 2138 2684 2912 4163 5779 6986 7603 7829 Size: 645 546 228 1251 1616 1207 617 226 Cuts at: 7829 7981 10090 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1616 1251 1207 645 617 546 228 226 152 AseI AT'TA_AT Cuts at: 160 1032 2049 5136 5178 8848 160 Size: 872 1017 3087 42 3670 1606 Fragments arranged by size: 3670 3087 1606 1017 872 42 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 10294 AvaI C'yCGr_G Cuts at: 1870 1943 7506 7619 1870 Size: 73 5563 113 4545 Fragments arranged by size: 5563 4545 113 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 7346 7379 7346 Size: 33 10261 BamHI G'GATC_C Cuts at: 5944 7624 7991 5944 Size: 1680 367 8247 Fragments arranged by size: 8247 1680 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4318 7492 7615 9070 618 Size: 325 217 627 2531 3174 123 1455 1842 Fragments arranged by size: 3174 2531 1842 1455 627 325 217 123 BanII G_rGCy'C Cuts at: 1391 5171 6588 6757 7317 7613 1391 Size: 3780 1417 169 560 296 4072 Fragments arranged by size: 4072 3780 1417 560 296 169 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3381 3840 4245 5288 5922 6150 6545 Size: 971 1482 459 405 1043 634 228 395 Cuts at: 6545 6753 928 Size: 208 4469 Fragments arranged by size: 4469 1482 1043 971 634 459 405 395 228 208 BbvCI CC'TCA_GC Cuts at: 3836 3836 Size: 10294 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 8485 8519 1903 Size: 34 6548 34 3678 Fragments arranged by size: 6548 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2508 4506 6190 8186 9396 2508 Size: 1998 1684 1996 1210 3406 Fragments arranged by size: 3406 1998 1996 1684 1210 BclI T'GATC_A Cuts at: 1548 2094 1548 Size: 546 9748 BfrBI ATG'CAT Cuts at: 5429 5429 Size: 10294 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3985 8905 136 Size: 107 122 71 631 605 2313 4920 1525 Fragments arranged by size: 4920 2313 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3025 4249 4289 6673 7281 10289 3025 Size: 1224 40 2384 608 3008 3030 Fragments arranged by size: 3030 3008 2384 1224 608 40 BlpI GC'TnA_GC Cuts at: 2941 5561 7711 2941 Size: 2620 2150 5524 Fragments arranged by size: 5524 2620 2150 Bme1580I G_kGCm'C Cuts at: 1790 7317 8355 9511 1790 Size: 5527 1038 1156 2573 Fragments arranged by size: 5527 2573 1156 1038 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 10294 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2721 7159 8025 Size: 416 53 83 399 24 1304 4438 866 Cuts at: 8025 8983 442 Size: 958 1753 Fragments arranged by size: 4438 1753 1304 958 866 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 5886 1056 Size: 4830 5464 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2108 4053 5155 6209 8954 1239 Size: 869 1945 1102 1054 2745 2579 Fragments arranged by size: 2745 2579 1945 1102 1054 869 Bpu10I CC'TnA_GC Cuts at: 3836 5601 6151 3836 Size: 1765 550 7979 Fragments arranged by size: 7979 1765 550 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2616 5110 6341 6561 8419 9284 9582 2616 Size: 2494 1231 220 1858 865 298 3328 Fragments arranged by size: 3328 2494 1858 1231 865 298 220 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3517 8957 882 Size: 617 2018 5440 2219 Fragments arranged by size: 5440 2219 2018 617 BsaAI yAC'GTr Cuts at: 493 1204 8085 493 Size: 711 6881 2702 Fragments arranged by size: 6881 2702 711 BsaBI GATnn'nnATC Cuts at: 10288 10288 Size: 10294 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2531 8101 8483 9118 Size: 53 83 186 936 998 5570 382 635 Cuts at: 9118 275 Size: 1451 Fragments arranged by size: 5570 1451 998 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 8727 9125 9399 9546 9957 8727 Size: 398 274 147 411 9064 Fragments arranged by size: 9064 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3447 3477 4257 4287 7209 Size: 30 165 30 2044 30 780 30 2922 Cuts at: 7209 7239 1178 Size: 30 4233 Fragments arranged by size: 4233 2922 2044 780 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3853 5789 6596 6623 7230 1186 Size: 2667 1936 807 27 607 4250 Fragments arranged by size: 4250 2667 1936 807 607 27 BseYI C'CCAG_C Cuts at: 3005 6872 9497 9993 3005 Size: 3867 2625 496 3306 Fragments arranged by size: 3867 3306 2625 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2781 4440 5923 7454 1163 Size: 573 1045 1659 1483 1531 4003 Fragments arranged by size: 4003 1659 1531 1483 1045 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 7744 8505 9533 1540 Size: 275 186 5743 761 1028 2301 Fragments arranged by size: 5743 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5078 6588 8355 8440 9511 5078 Size: 1510 1767 85 1071 5861 Fragments arranged by size: 5861 1767 1510 1071 85 BsmI GAATG_Cn' Cuts at: 3037 3138 5251 7805 7898 3037 Size: 101 2113 2554 93 5433 Fragments arranged by size: 5433 2554 2113 101 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2687 6142 2687 Size: 3455 6839 Bsp1286I G_dGCh'C Cuts at: 1391 1790 5078 5171 6588 6757 7317 7613 8355 Size: 399 3288 93 1417 169 560 296 742 Cuts at: 8355 8440 9511 1391 Size: 85 1071 2174 Fragments arranged by size: 3288 2174 1417 1071 742 560 399 296 169 93 85 BspEI T'CCGG_A Cuts at: 9957 9957 Size: 10294 BspHI T'CATG_A Cuts at: 3313 3661 8183 9913 3313 Size: 348 4522 1730 3694 Fragments arranged by size: 4522 3694 1730 348 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4033 6336 7649 844 Size: 3189 2303 1313 3489 Fragments arranged by size: 3489 3189 2303 1313 BsrBI CCG'CTC Cuts at: 1394 1823 8181 1394 Size: 429 6358 3507 Fragments arranged by size: 6358 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6891 8789 8963 65 Size: 1183 5643 1898 174 1396 Fragments arranged by size: 5643 1898 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 8938 9125 1700 Size: 110 7128 187 2869 Fragments arranged by size: 7128 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 4762 6999 96 Size: 4666 2237 3391 Fragments arranged by size: 4666 3391 2237 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 10294 BssSI C'ACGA_G Cuts at: 6768 6995 8354 9366 6768 Size: 227 1359 1012 7696 Fragments arranged by size: 7696 1359 1012 227 BstAPI GCAn_nnn'nTGC Cuts at: 794 2142 3076 6833 794 Size: 1348 934 3757 4255 Fragments arranged by size: 4255 3757 1348 934 BstBI TT'CG_AA Cuts at: 9098 9098 Size: 10294 BstEII G'GTnAC_C Cuts at: 1184 5606 7159 7501 1184 Size: 4422 1553 342 3977 Fragments arranged by size: 4422 3977 1553 342 BstXI CCAn_nnnn'nTGG Cuts at: 2086 6002 7446 7475 2086 Size: 3916 1444 29 4905 Fragments arranged by size: 4905 3916 1444 29 BstYI r'GATC_y Cuts at: 1972 2552 3025 4249 4289 5944 6673 7281 7575 Size: 580 473 1224 40 1655 729 608 294 Cuts at: 7575 7624 7991 8376 8393 9845 9931 9943 10289 Size: 49 367 385 17 1452 86 12 346 Cuts at: 10289 1972 Size: 1977 Fragments arranged by size: 1977 1655 1452 1224 729 608 580 473 385 367 346 294 86 49 40 17 12 BstZ17I GTA'TAC Cuts at: 4943 4943 Size: 10294 Bsu36I CC'TnA_GG Cuts at: 3546 5277 3546 Size: 1731 8563 BtgI C'CryG_G Cuts at: 513 1065 2004 6957 513 Size: 552 939 4953 3850 Fragments arranged by size: 4953 3850 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 9555 Fragments arranged by size: 9555 683 56 BtsI GCAGTG_nn' Cuts at: 2602 3949 4779 5911 7810 8604 8624 2602 Size: 1347 830 1132 1899 794 20 4272 Fragments arranged by size: 4272 1899 1347 1132 830 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 10294 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2114 2149 6624 6659 529 Size: 35 1550 35 4475 35 4164 Fragments arranged by size: 4475 4164 1550 35 35 35 DraI TTT'AAA Cuts at: 5113 7598 5113 Size: 2485 7809 DraIII CAC_nnn'GTG Cuts at: 2118 2118 Size: 10294 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 9301 809 Size: 507 7985 1802 Fragments arranged by size: 7985 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3300 7741 8630 Size: 54 1425 50 275 18 1470 4441 889 Cuts at: 8630 10259 8 Size: 1629 43 Fragments arranged by size: 4441 1629 1470 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 7741 1537 Size: 275 5929 4090 Fragments arranged by size: 5929 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2025 2250 3942 4037 4194 4635 5608 5637 Size: 672 225 1692 95 157 441 973 29 Cuts at: 5637 5661 6069 6316 6618 6719 7044 8224 1353 Size: 24 408 247 302 101 325 1180 3423 Fragments arranged by size: 3423 1692 1180 973 672 441 408 325 302 247 225 157 101 95 29 24 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3013 8881 9255 9401 387 Size: 1064 138 1424 5868 374 146 1280 Fragments arranged by size: 5868 1424 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 6586 6586 Size: 10294 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2108 2586 3126 3264 4053 4124 4257 Size: 393 476 478 540 138 789 71 133 Cuts at: 4257 5155 6162 6209 6539 6765 7068 7270 8357 Size: 898 1007 47 330 226 303 202 1087 Cuts at: 8357 8954 9741 10009 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1087 1007 898 789 787 597 540 478 476 393 330 303 268 226 202 138 133 71 47 EcoNI CCTnn'n_nnAGG Cuts at: 3531 5861 3531 Size: 2330 7964 EcoO109I rG'GnC_Cy Cuts at: 2174 4674 6325 7314 2174 Size: 2500 1651 989 5154 Fragments arranged by size: 5154 2500 1651 989 EcoRI G'AATT_C Cuts at: 2912 7603 7981 2912 Size: 4691 378 5225 Fragments arranged by size: 5225 4691 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 5576 5818 6327 5576 Size: 242 509 9543 Fragments arranged by size: 9543 509 242 FspI TGC'GCA Cuts at: 8800 8800 Size: 10294 HaeII r_GCGC'y Cuts at: 1678 4603 9441 1678 Size: 2925 4838 2531 Fragments arranged by size: 4838 2925 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2227 2259 2506 2538 3194 Size: 32 135 32 655 32 247 32 656 Cuts at: 3194 3200 3226 3232 5636 5668 5744 5776 6716 Size: 6 26 6 2404 32 76 32 940 Cuts at: 6716 6748 7209 7241 7376 7408 8935 8967 9009 Size: 32 461 32 135 32 1527 32 42 Cuts at: 9009 9041 1373 Size: 32 2626 Fragments arranged by size: 2626 2404 1527 940 656 655 461 247 135 135 76 42 32 32 32 32 32 32 32 32 32 32 32 26 6 6 HincII GTy'rAC Cuts at: 669 1657 1938 2246 4110 4516 6943 7638 7884 Size: 988 281 308 1864 406 2427 695 246 Cuts at: 7884 669 Size: 3079 Fragments arranged by size: 3079 2427 1864 988 695 406 308 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 10294 HpaI GTT'AAC Cuts at: 4110 4516 7884 4110 Size: 406 3368 6520 Fragments arranged by size: 6520 3368 406 KpnI G_GTAC'C Cuts at: 4322 7619 4322 Size: 3297 6997 MfeI C'AATT_G Cuts at: 6815 7422 7473 7893 6815 Size: 607 51 420 9216 Fragments arranged by size: 9216 607 420 51 MluI A'CGCG_T Cuts at: 10170 10170 Size: 10294 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2491 5707 7500 9408 9592 1305 Size: 1186 3216 1793 1908 184 2007 Fragments arranged by size: 3216 2007 1908 1793 1186 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3302 10 Size: 54 1425 343 1470 7002 Fragments arranged by size: 7002 1470 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3864 4541 5859 6115 6962 7325 8252 Size: 648 2698 677 1318 256 847 363 927 Cuts at: 8252 8611 8770 518 Size: 359 159 2042 Fragments arranged by size: 2698 2042 1318 927 847 677 648 363 359 256 159 MspA1I CmG'CkG Cuts at: 1606 2512 2765 6619 6848 7360 7710 8387 9535 Size: 906 253 3854 229 512 350 677 1148 Cuts at: 9535 9780 1606 Size: 245 2120 Fragments arranged by size: 3854 2120 1148 906 677 512 350 253 245 229 NcoI C'CATG_G Cuts at: 513 1065 2004 6957 513 Size: 552 939 4953 3850 Fragments arranged by size: 4953 3850 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 10294 NheI G'CTAG_C Cuts at: 1052 5882 1052 Size: 4830 5464 NotI GC'GGCC_GC Cuts at: 7741 7741 Size: 10294 NsiI A_TGCA'T Cuts at: 5431 5431 Size: 10294 NspI r_CATG'y Cuts at: 1132 1229 2149 2816 2988 6535 7330 7652 9197 Size: 97 920 667 172 3547 795 322 1545 Cuts at: 9197 1132 Size: 2229 Fragments arranged by size: 3547 2229 1545 920 795 667 322 172 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 10294 PciI A'CATG_T Cuts at: 2984 9193 2984 Size: 6209 4085 PflMI CCAn_nnn'nTGG Cuts at: 1108 2386 7552 1108 Size: 1278 5166 3850 Fragments arranged by size: 5166 3850 1278 PfoI T'CCnGG_A Cuts at: 4627 5299 4627 Size: 672 9622 PmeI GTTT'AAAC Cuts at: 7598 7598 Size: 10294 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 8363 8395 9876 9908 8363 Size: 32 1481 32 8749 Fragments arranged by size: 8749 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4674 6325 4674 Size: 1651 8643 PshAI GACnn'nnGTC Cuts at: 1613 2534 1613 Size: 921 9373 PsiI TTA'TAA Cuts at: 7864 7864 Size: 10294 PspOMI G'GGCC_C Cuts at: 7313 7313 Size: 10294 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3231 3263 3231 Size: 32 10262 PstI C_TGCA'G Cuts at: 830 4786 5816 6448 7646 830 Size: 3956 1030 632 1198 3478 Fragments arranged by size: 3956 3478 1198 1030 632 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 10294 PvuII CAG'CTG Cuts at: 2765 6619 6848 7360 2765 Size: 3854 229 512 5699 Fragments arranged by size: 5699 3854 512 229 SacI G_AGCT'C Cuts at: 6588 6588 Size: 10294 SalI G'TCGA_C Cuts at: 1936 7636 1936 Size: 5700 4594 SapI GCTCTTCn'nnn_ Cuts at: 2025 2250 5661 6069 2025 Size: 225 3411 408 6250 Fragments arranged by size: 6250 3411 408 225 SbfI CC_TGCA'GG Cuts at: 7646 7646 Size: 10294 ScaI AGT'ACT Cuts at: 5155 8542 10254 5155 Size: 3387 1712 5195 Fragments arranged by size: 5195 3387 1712 SexAI A'CCwGG_T Cuts at: 4120 4321 5820 4120 Size: 201 1499 8594 Fragments arranged by size: 8594 1499 201 SfcI C'TryA_G Cuts at: 826 1045 2392 2417 4293 4782 5812 6444 7642 Size: 219 1347 25 1876 489 1030 632 1198 Cuts at: 7642 8777 9458 9649 826 Size: 1135 681 191 1471 Fragments arranged by size: 1876 1471 1347 1198 1135 1030 681 632 489 219 191 25 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 10294 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 10294 SmaI CCC'GGG Cuts at: 1872 7621 1872 Size: 5749 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2631 3424 5125 6320 6576 8398 Size: 197 926 688 793 1701 1195 256 1822 Cuts at: 8398 9299 9561 820 Size: 901 262 1553 Fragments arranged by size: 1822 1701 1553 1195 926 901 793 688 262 256 197 SnaBI TAC'GTA Cuts at: 493 8085 493 Size: 7592 2702 SpeI A'CTAG_T Cuts at: 152 3877 5492 152 Size: 3725 1615 4954 Fragments arranged by size: 4954 3725 1615 SphI G_CATG'C Cuts at: 1132 2149 7652 1132 Size: 1017 5503 3774 Fragments arranged by size: 5503 3774 1017 SspI AAT'ATT Cuts at: 5 52 3269 3370 3384 3646 5134 5829 7112 Size: 47 3217 101 14 262 1488 695 1283 Cuts at: 7112 8218 5 Size: 1106 2081 Fragments arranged by size: 3217 2081 1488 1283 1106 695 262 101 47 14 StuI AGG'CCT Cuts at: 1517 3537 6417 1517 Size: 2020 2880 5394 Fragments arranged by size: 5394 2880 2020 StyI C'CwwG_G Cuts at: 513 1065 2004 2479 3116 4437 6665 6957 7243 Size: 552 939 475 637 1321 2228 292 286 Cuts at: 7243 7402 7733 513 Size: 159 331 3074 Fragments arranged by size: 3074 2228 1321 939 637 552 475 331 292 286 159 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5600 5954 6105 7147 7434 7504 8321 Size: 1255 4020 354 151 1042 287 70 817 Cuts at: 8321 8338 8491 8676 9121 325 Size: 17 153 185 445 1498 Fragments arranged by size: 4020 1498 1255 1042 817 445 354 287 185 153 151 70 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2035 3911 3966 Size: 274 80 33 51 1443 58 1876 55 Cuts at: 3966 4762 5066 5153 6999 8540 10252 96 Size: 796 304 87 1846 1541 1712 138 Fragments arranged by size: 1876 1846 1712 1541 1443 796 304 274 138 87 80 58 55 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4658 4690 5929 5961 10254 10286 4658 Size: 32 1239 32 4293 32 4666 Fragments arranged by size: 4666 4293 1239 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 7165 1316 Size: 5849 4445 XbaI T'CTAG_A Cuts at: 6676 7630 6676 Size: 954 9340 XcmI CCAnnnn_n'nnnnTGG Cuts at: 7339 7339 Size: 10294 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 10294 XmaI C'CCGG_G Cuts at: 1870 7619 1870 Size: 5749 4545 XmnI GAAnn'nnTTC Cuts at: 3111 3342 4583 5205 5352 5670 8423 3111 Size: 231 1241 622 147 318 2753 4982 Fragments arranged by size: 4982 2753 1241 622 318 231 147 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2532 8102 276 Size: 53 83 186 936 998 5570 2468 Fragments arranged by size: 5570 2468 998 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbeI BplI BsiWI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI RsrII SacII SanDI SfiI SfoI SrfI SwaI