Restriction map for
pFN21AB5544
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# NO CUT ENZYMES ( 72 enzymes) #
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Acc65I AclI AflII AflIII AgeI
AocI AscI Asp718I AvrII BbeI
BbrPI BciVI BfrI BglII BlnI
BsaAI BsaBI BsiWI Bsp1407I BspDI
BspLU11I BsrGI BssHII Bst1107I BstBI
BstZ17I Bsu36I ClaI CpoI DraI
Eco81I EcoRI EcoRV EcoT22I FseI
HindIII KasI KpnI MamI MfeI
MluI MunI NarI NheI NotI
NruI NsiI NspV PacI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RsrII SalI SbfI SexAI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SwaI XbaI
XmaCI XmaI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AatII 1440 GACGTC GACGT^C
AcyI 1440 GACGTC G[GA]^CG[CT]C
AhdI 322 GACAATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3172 GTGCAC G^TGCAC
Aor51HI 2567 AGCGCT AGC^GCT
ApaLI 3172 GTGCAC G^TGCAC
AspEI 322 GACAATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1292 TGCGCA TGC^GCA
BcgI 1538 GCACGCTGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 714 CCGGG CC^[GC]GG
BsaHI 1440 GACGTC G[GA]^CG[CT]C
BsiEI 1619 CGGCCG CG[GA][CT]^CG
BsmI 2601 GCATTC GAATGC(1/-1)
BspMI 1089 GCAGGT ACCTGC(4/8)
BstXI 459 CCACAGCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 391 GACTATTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1619 CGGCCG C^GGCCG
Eam1105I 322 GACAATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1619 CGGCCG C^GGCCG
Eco47III 2567 AGCGCT AGC^GCT
Eco52I 1619 CGGCCG C^GGCCG
FspI 1292 TGCGCA TGC^GCA
HaeII 2567 AGCGCT [GA]GCGC^[CT]
Hin1I 1440 GACGTC G[GA]^CG[CT]C
KspI 2106 CCGCGG CCGC^GG
NciI 714 CCGGG CC^[GC]GG
NdeI 2654 CATATG CA^TATG
SacI 2515 GAGCTC GAGCT^C
SacII 2106 CCGCGG CCGC^GG
SapI 2940 GAAGAGC GCTCTTC(1/4)
ScaI 3111 AGTACT AGT^ACT
SfiI 1512 GGCCACAGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 3224 GCATGC GCATG^C
SspI 1341 AATATT AAT^ATT
XcmI 1664 CCACAGAGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 32 enzymes) #
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AccIII 809 TCCGGA T^CCGGA
2463 TCCGGA
AcsI 445 GAATTT [GA]^AATT[CT]
3655 GAATTT
ApoI 445 GAATTT [GA]^AATT[CT]
3655 GAATTT
AseI 2719 ATTAAT AT^TAAT
3616 ATTAAT
AsnI 2719 ATTAAT AT^TAAT
3616 ATTAAT
BamHI 642 GGATCC G^GATCC
699 GGATCC
BbvCI 1625 CCTCAGC CCTCAGC(-5/-2)
3134 CCTCAGC
BglI 1402 GCCGACATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1513 GCCACAGTGGC
BlpI 470 GCTCAGC GC^T[ACGT]AGC
4221 GCTTAGC
Bpu1102I 470 GCTCAGC GC^T[ACGT]AGC
4221 GCTTAGC
BsaI 688 GAGACC GGTCTC(1/5)
813 GAGACC
BsaWI 809 TCCGGA [AT]^CCGG[AT]
2463 TCCGGA
BseAI 809 TCCGGA T^CCGGA
2463 TCCGGA
BsmBI 1385 CGTCTC CGTCTC(1/5)
1883 CGTCTC
BspEI 809 TCCGGA T^CCGGA
2463 TCCGGA
BsrBI 1774 GAGCGG CCGCTC(-3/-3)
1849 GAGCGG
BsrFI 1549 GCCGGC [GA]^CCGG[CT]
1984 GCCGGC
BssSI 115 CACGAG CACGAG(-5/-1)
1783 CACGAG
BstAPI 927 GCAGCGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3672 GCATCCTCTGC
CelII 470 GCTCAGC GC^T[ACGT]AGC
4221 GCTTAGC
Cfr10I 1549 GCCGGC [GA]^CCGG[CT]
1984 GCCGGC
DraIII 1593 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
3471 CACACAGTG
HgaI 930 GCGTC GACGC(5/10)
1384 GCGTC
HpaI 3256 GTTAAC GTT^AAC
3406 GTTAAC
MroI 809 TCCGGA T^CCGGA
2463 TCCGGA
NaeI 1549 GCCGGC GCC^GGC
1984 GCCGGC
NcoI 596 CCATGG C^CATGG
1472 CCATGG
NgoMIV 1549 GCCGGC G^CCGGC
1984 GCCGGC
PaeR7I 2423 CTCGAG C^TCGAG
3569 CTCGAG
PpuMI 716 GGGACCT [GA]G^G[AT]CC[CT]
2775 AGGACCT
PshBI 2719 ATTAAT AT^TAAT
3616 ATTAAT
XhoI 2423 CTCGAG C^TCGAG
3569 CTCGAG
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# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccI AccII AciI AfaI AluI
AlwI AlwNI ApaI Asp700I AspHI
AspI AvaI AvaII BalI BanI
BanII BbsI BbvI BclI BfaI
BmyI BpmI BpuAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspHI BsrDI
BsrI BssKI BstEII BstF5I BstNI
BstPI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoNI EcoO109I EcoO65I
EcoRII EcoT14I FauI FbaI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NdeII NlaIII
NlaIV NspI PflFI PflMI PleI
PstI PvuII RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XhoII XmnI