(Circular) (Six-base) MAPSORT of: pFN21AB5544.seq Check: 5443 from: 1 to: 9040 pFN21AB5544 9040 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3085 3085 Size: 9040 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3449 6850 278 Size: 53 83 186 936 1913 3401 2468 Fragments arranged by size: 3401 2468 1913 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2419 4793 6273 6383 1937 Size: 482 2374 1480 110 4594 Fragments arranged by size: 4594 2374 1480 482 110 Acc65I G'GTAC_C Cuts at: 6361 6361 Size: 9040 AclI AA'CG_TT Cuts at: 1563 7167 7540 1563 Size: 5604 373 3063 Fragments arranged by size: 5604 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2151 3387 4149 4964 5300 5400 5535 7103 Size: 519 1236 762 815 336 100 135 1568 Cuts at: 7103 8487 8755 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1568 1384 1236 815 762 519 336 268 135 100 AfeI AGC'GCT Cuts at: 4574 4574 Size: 9040 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8843 AflIII A'CryG_T Cuts at: 1203 7939 8916 1203 Size: 6736 977 1327 Fragments arranged by size: 6736 1327 977 AgeI A'CCGG_T Cuts at: 7871 7871 Size: 9040 AhdI GACnn_n'nnGTC Cuts at: 2332 7769 2332 Size: 5437 3603 AleI CACnn'nnGTG Cuts at: 1166 2782 4028 4515 5184 1166 Size: 1616 1246 487 669 5022 Fragments arranged by size: 5022 1616 1246 669 487 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2159 5202 1184 Size: 975 3043 5022 Fragments arranged by size: 5022 3043 975 AlwNI CAG_nnn'CTG Cuts at: 2308 2938 3076 6115 6314 6783 8355 2308 Size: 630 138 3039 199 469 1572 2993 Fragments arranged by size: 3039 2993 1572 630 469 199 138 ApaI G_GGCC'C Cuts at: 2116 3497 3696 5168 2116 Size: 1381 199 1472 5988 Fragments arranged by size: 5988 1472 1381 199 ApaLI G'TGCA_C Cuts at: 5177 7097 8253 5177 Size: 1920 1156 5964 Fragments arranged by size: 5964 1920 1156 ApoI r'AATT_y Cuts at: 1493 2450 5660 6349 6575 6727 8836 1493 Size: 957 3210 689 226 152 2109 1697 Fragments arranged by size: 3210 2109 1697 957 689 226 152 AseI AT'TA_AT Cuts at: 160 1032 4725 5622 7594 160 Size: 872 3693 897 1972 1606 Fragments arranged by size: 3693 1972 1606 897 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9040 AvaI C'yCGr_G Cuts at: 1870 1943 3427 4428 5099 5574 6365 1870 Size: 73 1484 1001 671 475 791 4545 Fragments arranged by size: 4545 1484 1001 791 671 475 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2749 2782 5367 5400 2749 Size: 33 2585 33 6389 Fragments arranged by size: 6389 2585 33 33 BamHI G'GATC_C Cuts at: 2647 2704 6370 6737 2647 Size: 57 3666 367 4950 Fragments arranged by size: 4950 3666 367 57 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2195 3731 5207 6361 7816 Size: 325 217 627 408 1536 1476 1154 1455 Cuts at: 7816 618 Size: 1842 Fragments arranged by size: 1842 1536 1476 1455 1154 627 408 325 217 BanII G_rGCy'C Cuts at: 1391 2116 2486 3497 3696 3873 4524 5168 6359 Size: 725 370 1011 199 177 651 644 1191 Cuts at: 6359 1391 Size: 4072 Fragments arranged by size: 4072 1191 1011 725 651 644 370 199 177 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2328 2803 3197 3426 4137 4419 4882 Size: 971 429 475 394 229 711 282 463 Cuts at: 4882 928 Size: 5086 Fragments arranged by size: 5086 971 711 475 463 429 394 282 229 BbvCI CC'TCA_GC Cuts at: 3631 5140 3631 Size: 1509 7531 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3532 3566 7231 7265 1903 Size: 34 50 34 1511 34 3665 34 3678 Fragments arranged by size: 3678 3665 1511 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6932 8142 6932 Size: 1210 7830 BclI T'GATC_A Cuts at: 1548 4663 4888 6159 1548 Size: 3115 225 1271 4429 Fragments arranged by size: 4429 3115 1271 225 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3413 3524 7651 Size: 107 122 71 631 605 1741 111 4127 Cuts at: 7651 136 Size: 1525 Fragments arranged by size: 4127 1741 1525 631 605 122 111 107 71 BglII A'GATC_T Cuts at: 9035 9035 Size: 9040 BlpI GC'TnA_GC Cuts at: 2476 6227 6457 2476 Size: 3751 230 5059 Fragments arranged by size: 5059 3751 230 Bme1580I G_kGCm'C Cuts at: 1790 2116 3384 3497 3696 3734 4035 5168 5181 Size: 326 1268 113 199 38 301 1133 13 Cuts at: 5181 6094 7101 8257 1790 Size: 913 1007 1156 2573 Fragments arranged by size: 2573 1268 1156 1133 1007 913 326 301 199 113 38 13 BmgBI CAC'GTC Cuts at: 1318 2223 1318 Size: 905 8135 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2191 2224 2467 Size: 416 53 83 399 24 774 33 243 Cuts at: 2467 3026 3495 6771 7729 442 Size: 559 469 3276 958 1753 Fragments arranged by size: 3276 1753 958 774 559 469 416 399 243 83 53 33 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9040 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3826 3826 Size: 9040 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3124 3487 3672 4182 4309 4831 6137 6198 Size: 1885 363 185 510 127 522 1306 61 Cuts at: 6198 7700 1239 Size: 1502 2579 Fragments arranged by size: 2579 1885 1502 1306 522 510 363 185 127 61 Bpu10I CC'TnA_GC Cuts at: 2208 3631 5140 2208 Size: 1423 1509 6108 Fragments arranged by size: 6108 1509 1423 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2867 7165 8030 8328 2867 Size: 4298 865 298 3579 Fragments arranged by size: 4298 3579 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2687 2812 7703 882 Size: 617 1188 125 4891 2219 Fragments arranged by size: 4891 2219 1188 617 125 BsaAI yAC'GTr Cuts at: 493 1204 6831 493 Size: 711 5627 2702 Fragments arranged by size: 5627 2702 711 BsaBI GATnn'nnATC Cuts at: 9034 9034 Size: 9040 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3446 6847 7229 7864 Size: 53 83 186 936 1913 3401 382 635 Cuts at: 7864 275 Size: 1451 Fragments arranged by size: 3401 1913 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2814 4468 7473 7871 8145 8292 8703 2814 Size: 1654 3005 398 274 147 411 3151 Fragments arranged by size: 3151 3005 1654 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3137 3167 3179 3209 3376 Size: 30 165 30 1734 30 12 30 167 Cuts at: 3376 3406 4282 4312 1178 Size: 30 876 30 5906 Fragments arranged by size: 5906 1734 876 167 165 30 30 30 30 30 30 12 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3490 3574 3806 3866 4064 4377 5146 5336 Size: 2304 84 232 60 198 313 769 190 Cuts at: 5336 1186 Size: 4890 Fragments arranged by size: 4890 2304 769 313 232 198 190 84 60 BseYI C'CCAG_C Cuts at: 3593 3872 6108 8243 8739 3593 Size: 279 2236 2135 496 3894 Fragments arranged by size: 3894 2236 2135 496 279 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2102 2165 2625 3089 5384 1163 Size: 573 366 63 460 464 2295 4819 Fragments arranged by size: 4819 2295 573 464 460 366 63 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3627 6490 7251 8279 1540 Size: 275 186 1626 2863 761 1028 2301 Fragments arranged by size: 2863 2301 1626 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2067 2214 3789 4281 4524 4952 5181 5515 5575 Size: 147 1575 492 243 428 229 334 60 Cuts at: 5575 7101 7186 8257 2067 Size: 1526 85 1071 2850 Fragments arranged by size: 2850 1575 1526 1071 492 428 334 243 229 147 85 60 BsmI GAATG_Cn' Cuts at: 4606 6551 6644 4606 Size: 1945 93 7002 Fragments arranged by size: 7002 1945 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3396 3894 3396 Size: 498 8542 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2067 2116 2214 2486 3384 3497 3696 Size: 399 277 49 98 272 898 113 199 Cuts at: 3696 3734 3789 3873 4035 4281 4524 4952 5168 Size: 38 55 84 162 246 243 428 216 Cuts at: 5168 5181 5515 5575 6094 6359 7101 7186 8257 Size: 13 334 60 519 265 742 85 1071 Cuts at: 8257 1391 Size: 2174 Fragments arranged by size: 2174 1071 898 742 519 428 399 334 277 272 265 246 243 216 199 162 113 98 85 84 60 55 49 38 13 BspEI T'CCGG_A Cuts at: 2814 4468 8703 2814 Size: 1654 4235 3151 Fragments arranged by size: 4235 3151 1654 BspHI T'CATG_A Cuts at: 2683 3138 6052 6929 8659 2683 Size: 455 2914 877 1730 3064 Fragments arranged by size: 3064 2914 1730 877 455 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3085 6395 844 Size: 2241 3310 3489 Fragments arranged by size: 3489 3310 2241 BsrBI CCG'CTC Cuts at: 1394 1823 3781 3856 6927 1394 Size: 429 1958 75 3071 3507 Fragments arranged by size: 3507 3071 1958 429 75 BsrDI GCAATG_nn' Cuts at: 65 1248 2476 4269 4767 5591 5662 6228 7535 Size: 1183 1228 1793 498 824 71 566 1307 Cuts at: 7535 7709 65 Size: 174 1396 Fragments arranged by size: 1793 1396 1307 1228 1183 824 566 498 174 71 BsrFI r'CCGG_y Cuts at: 1700 1810 3554 3989 7684 7871 1700 Size: 110 1744 435 3695 187 2869 Fragments arranged by size: 3695 2869 1744 435 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 9040 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9040 BssSI C'ACGA_G Cuts at: 2120 3788 7100 8112 2120 Size: 1668 3312 1012 3048 Fragments arranged by size: 3312 3048 1668 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2938 5683 794 Size: 2144 2745 4151 Fragments arranged by size: 4151 2745 2144 BstBI TT'CG_AA Cuts at: 7844 7844 Size: 9040 BstEII G'GTnAC_C Cuts at: 1184 3535 3835 4015 1184 Size: 2351 300 180 6209 Fragments arranged by size: 6209 2351 300 180 BstXI CCAn_nnnn'nTGG Cuts at: 2471 2471 Size: 9040 BstYI r'GATC_y Cuts at: 1972 2148 2647 2704 5135 6370 6737 7122 7139 Size: 176 499 57 2431 1235 367 385 17 Cuts at: 7139 8591 8677 8689 9035 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 2431 1977 1452 1235 499 385 367 346 176 86 57 17 12 BtgI C'CryG_G Cuts at: 513 1065 2601 2659 2871 3477 4111 513 Size: 552 1536 58 212 606 634 5442 Fragments arranged by size: 5442 1536 634 606 552 212 58 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 8301 Fragments arranged by size: 8301 683 56 BtsI GCAGTG_nn' Cuts at: 2148 3089 3948 4596 5179 5191 6556 7350 7370 Size: 941 859 648 583 12 1365 794 20 Cuts at: 7370 2148 Size: 3818 Fragments arranged by size: 3818 1365 941 859 794 648 583 20 12 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9040 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 5478 5513 529 Size: 35 4914 35 4056 Fragments arranged by size: 4914 4056 35 35 DraI TTT'AAA Cuts at: 6344 6344 Size: 9040 DraIII CAC_nnn'GTG Cuts at: 3603 5481 3603 Size: 1878 7162 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2402 8047 809 Size: 507 1086 5645 1802 Fragments arranged by size: 5645 1802 1086 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2604 3624 4898 Size: 54 1425 50 275 18 774 1020 1274 Cuts at: 4898 5792 6487 7376 9005 8 Size: 894 695 889 1629 43 Fragments arranged by size: 1629 1425 1274 1020 894 889 774 695 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3624 6487 1537 Size: 275 1812 2863 4090 Fragments arranged by size: 4090 2863 1812 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3406 3821 4940 5554 6012 6970 1353 Size: 2053 415 1119 614 458 958 3423 Fragments arranged by size: 3423 2053 1119 958 614 458 415 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2953 3990 7627 8001 8147 387 Size: 1064 138 1364 1037 3637 374 146 1280 Fragments arranged by size: 3637 1364 1280 1064 1037 374 146 138 EcoICRI GAG'CTC Cuts at: 4522 4522 Size: 9040 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2151 3124 3387 3487 3672 4149 4182 Size: 393 519 973 263 100 185 477 33 Cuts at: 4182 4309 4831 4964 5300 5400 5535 6137 6198 Size: 127 522 133 336 100 135 602 61 Cuts at: 6198 7103 7700 8487 8755 1239 Size: 905 597 787 268 1524 Fragments arranged by size: 1524 973 905 787 602 597 522 519 477 393 336 268 263 185 135 133 127 100 100 61 33 EcoNI CCTnn'n_nnAGG Cuts at: 4197 4692 6028 4197 Size: 495 1336 7209 Fragments arranged by size: 7209 1336 495 EcoO109I rG'GnC_Cy Cuts at: 2112 2113 2722 3494 3692 4781 5165 2112 Size: 1 609 772 198 1089 384 5987 Fragments arranged by size: 5987 1089 772 609 384 198 1 EcoRI G'AATT_C Cuts at: 6349 6727 6349 Size: 378 8662 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2713 5381 6242 2713 Size: 2668 861 5511 Fragments arranged by size: 5511 2668 861 FspI TGC'GCA Cuts at: 3299 7546 3299 Size: 4247 4793 HaeII r_GCGC'y Cuts at: 1678 4576 8187 1678 Size: 2898 3611 2531 Fragments arranged by size: 3611 2898 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2318 2350 4207 4239 5050 Size: 32 135 32 746 32 1857 32 811 Cuts at: 5050 5082 5707 5739 5847 5879 7681 7713 7755 Size: 32 625 32 108 32 1802 32 42 Cuts at: 7755 7787 1373 Size: 32 2626 Fragments arranged by size: 2626 1857 1802 811 746 625 135 108 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2094 5263 5413 6384 6630 669 Size: 988 281 156 3169 150 971 246 3079 Fragments arranged by size: 3169 3079 988 971 281 246 156 150 HindIII A'AGCT_T Cuts at: 748 748 Size: 9040 HpaI GTT'AAC Cuts at: 5263 5413 6630 5263 Size: 150 1217 7673 Fragments arranged by size: 7673 1217 150 KpnI G_GTAC'C Cuts at: 6365 6365 Size: 9040 MfeI C'AATT_G Cuts at: 6639 6639 Size: 9040 MluI A'CGCG_T Cuts at: 8916 8916 Size: 9040 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3266 5334 8154 8338 1305 Size: 1961 2068 2820 184 2007 Fragments arranged by size: 2820 2068 2007 1961 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2606 4900 5794 10 Size: 54 1425 343 774 2294 894 3256 Fragments arranged by size: 3256 2294 1425 894 774 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2469 2782 3059 3065 4028 4274 4404 Size: 648 1303 313 277 6 963 246 130 Cuts at: 4404 4515 4847 5184 5406 5744 6998 7357 7516 Size: 111 332 337 222 338 1254 359 159 Cuts at: 7516 518 Size: 2042 Fragments arranged by size: 2042 1303 1254 963 648 359 338 337 332 313 277 246 222 159 130 111 6 MspA1I CmG'CkG Cuts at: 1606 2235 2480 3162 4113 4160 5398 6456 7133 Size: 629 245 682 951 47 1238 1058 677 Cuts at: 7133 8281 8526 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1238 1148 1058 951 682 677 629 245 245 47 NaeI GCC'GGC Cuts at: 3556 3991 3556 Size: 435 8605 NcoI C'CATG_G Cuts at: 513 1065 2601 3477 513 Size: 552 1536 876 6076 Fragments arranged by size: 6076 1536 876 552 NdeI CA'TA_TG Cuts at: 387 4660 387 Size: 4273 4767 NgoMIV G'CCGG_C Cuts at: 3554 3989 3554 Size: 435 8605 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9040 NotI GC'GGCC_GC Cuts at: 6487 6487 Size: 9040 NspI r_CATG'y Cuts at: 1132 1229 3298 4964 5233 6398 7943 1132 Size: 97 2069 1666 269 1165 1545 2229 Fragments arranged by size: 2229 2069 1666 1545 1165 269 97 PasI CC'CwG_GG Cuts at: 1348 2740 1348 Size: 1392 7648 PciI A'CATG_T Cuts at: 7939 7939 Size: 9040 PflMI CCAn_nnn'nTGG Cuts at: 1108 5597 5767 6115 1108 Size: 4489 170 348 4033 Fragments arranged by size: 4489 4033 348 170 PfoI T'CCnGG_A Cuts at: 2718 2978 3101 2718 Size: 260 123 8657 Fragments arranged by size: 8657 260 123 PmeI GTTT'AAAC Cuts at: 6344 6344 Size: 9040 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2298 2330 5531 5563 7109 7141 8622 8654 2298 Size: 32 3201 32 1546 32 1481 32 2684 Fragments arranged by size: 3201 2684 1546 1481 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2722 4781 2722 Size: 2059 6981 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 9040 PsiI TTA'TAA Cuts at: 5669 6610 5669 Size: 941 8099 PspOMI G'GGCC_C Cuts at: 2112 3493 3692 5164 2112 Size: 1381 199 1472 5988 Fragments arranged by size: 5988 1472 1381 199 PspXI vC'TCGA_Gb Cuts at: 4428 5574 4428 Size: 1146 7894 PstI C_TGCA'G Cuts at: 830 3096 4603 5186 6392 830 Size: 2266 1507 583 1206 3478 Fragments arranged by size: 3478 2266 1507 1206 583 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9040 PvuII CAG'CTG Cuts at: 2235 3162 5398 2235 Size: 927 2236 5877 Fragments arranged by size: 5877 2236 927 SacI G_AGCT'C Cuts at: 4524 4524 Size: 9040 SacII CC_GC'GG Cuts at: 4114 4114 Size: 9040 SalI G'TCGA_C Cuts at: 1936 6382 1936 Size: 4446 4594 SapI GCTCTTCn'nnn_ Cuts at: 4940 4940 Size: 9040 SbfI CC_TGCA'GG Cuts at: 6392 6392 Size: 9040 ScaI AGT'ACT Cuts at: 5118 7288 9000 5118 Size: 2170 1712 5158 Fragments arranged by size: 5158 2170 1712 SfcI C'TryA_G Cuts at: 826 1045 3092 3157 4599 5182 6388 7523 8204 Size: 219 2047 65 1442 583 1206 1135 681 Cuts at: 8204 8395 826 Size: 191 1471 Fragments arranged by size: 2047 1471 1442 1206 1135 681 583 219 191 65 SfiI GGCCn_nnn'nGGCC Cuts at: 3524 3524 Size: 9040 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9040 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9040 SmaI CCC'GGG Cuts at: 1872 6367 1872 Size: 4495 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2846 4428 5574 7144 8045 8307 Size: 197 926 903 1582 1146 1570 901 262 Cuts at: 8307 820 Size: 1553 Fragments arranged by size: 1582 1570 1553 1146 926 903 901 262 197 SnaBI TAC'GTA Cuts at: 493 6831 493 Size: 6338 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 9040 SphI G_CATG'C Cuts at: 1132 5233 6398 1132 Size: 4101 1165 3774 Fragments arranged by size: 4101 3774 1165 SspI AAT'ATT Cuts at: 5 52 3348 6964 5 Size: 47 3296 3616 2081 Fragments arranged by size: 3616 3296 2081 47 StuI AGG'CCT Cuts at: 1517 3677 3999 5825 6023 6175 1517 Size: 2160 322 1826 198 152 4382 Fragments arranged by size: 4382 2160 1826 322 198 152 StyI C'CwwG_G Cuts at: 513 1065 2601 3352 3477 3678 3717 4314 4777 Size: 552 1536 751 125 201 39 597 463 Cuts at: 4777 5031 6479 513 Size: 254 1448 3074 Fragments arranged by size: 3074 1536 1448 751 597 552 463 254 201 125 39 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3067 3144 3148 4040 4134 4790 7067 Size: 1255 1487 77 4 892 94 656 2277 Cuts at: 7067 7084 7237 7422 7867 325 Size: 17 153 185 445 1498 Fragments arranged by size: 2277 1498 1487 1255 892 656 445 185 153 94 77 17 4 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4577 5116 5328 Size: 274 80 33 51 1443 2600 539 212 Cuts at: 5328 5787 7286 8998 96 Size: 459 1499 1712 138 Fragments arranged by size: 2600 1712 1499 1443 539 459 274 212 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3376 3408 4138 4170 4830 4862 9000 9032 3376 Size: 32 730 32 660 32 4138 32 3384 Fragments arranged by size: 4138 3384 730 660 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2888 4013 5924 1316 Size: 1572 1125 1911 4432 Fragments arranged by size: 4432 1911 1572 1125 XbaI T'CTAG_A Cuts at: 6376 6376 Size: 9040 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3676 3676 Size: 9040 XhoI C'TCGA_G Cuts at: 1943 4428 5574 1943 Size: 2485 1146 5409 Fragments arranged by size: 5409 2485 1146 XmaI C'CCGG_G Cuts at: 1870 6365 1870 Size: 4495 4545 XmnI GAAnn'nnTTC Cuts at: 4449 4485 5361 5548 5702 7169 4449 Size: 36 876 187 154 1467 6320 Fragments arranged by size: 6320 1467 876 187 154 36 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3447 6848 276 Size: 53 83 186 936 1913 3401 2468 Fragments arranged by size: 3401 2468 1913 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BbeI BfrBI BsiWI BstZ17I Bsu36I EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PsrI RsrII SanDI SexAI SfoI SrfI SwaI