Restriction map for
pFN21AB5544
(Linear) (Six-base) MAPSORT of: KIBB5544.seq Check: 515 from: 1 to: 4335
KIBB5544.orf
With 182 enzymes: SgfI *
October 24, 2006 15:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1080 4335
Size: 1080 3255
AatII G_ACGT'C
Cuts at: 0 1444 4335
Size: 1444 2891
AccI GT'mk_AC
Cuts at: 0 414 2788 4268 4335
Size: 414 2374 1480 67
Fragments arranged by size:
2374 1480 414 67
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 146 1382 2144 2959 3295 3395 3530 4335
Size: 146 1236 762 815 336 100 135 805
Fragments arranged by size:
1236 815 805 762 336 146 135 100
AfeI AGC'GCT
Cuts at: 0 2569 4335
Size: 2569 1766
AhdI GACnn_n'nnGTC
Cuts at: 0 327 4335
Size: 327 4008
AleI CACnn'nnGTG
Cuts at: 0 777 2023 2510 3179 4335
Size: 777 1246 487 669 1156
Fragments arranged by size:
1246 1156 777 669 487
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 154 3197 4335
Size: 154 3043 1138
Fragments arranged by size:
3043 1138 154
AlwNI CAG_nnn'CTG
Cuts at: 0 303 933 1071 4110 4309 4335
Size: 303 630 138 3039 199 26
Fragments arranged by size:
3039 630 303 199 138 26
ApaI G_GGCC'C
Cuts at: 0 111 1492 1691 3163 4335
Size: 111 1381 199 1472 1172
Fragments arranged by size:
1472 1381 1172 199 111
ApaLI G'TGCA_C
Cuts at: 0 3172 4335
Size: 3172 1163
ApoI r'AATT_y
Cuts at: 0 445 3655 4335
Size: 445 3210 680
Fragments arranged by size:
3210 680 445
AseI AT'TA_AT
Cuts at: 0 2720 3617 4335
Size: 2720 897 718
Fragments arranged by size:
2720 897 718
AvaI C'yCGr_G
Cuts at: 0 1422 2423 3094 3569 4335
Size: 1422 1001 671 475 766
Fragments arranged by size:
1422 1001 766 671 475
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 744 777 3362 3395 4335
Size: 744 33 2585 33 940
Fragments arranged by size:
2585 940 744 33 33
BamHI G'GATC_C
Cuts at: 0 642 699 4335
Size: 642 57 3636
Fragments arranged by size:
3636 642 57
BanI G'GyrC_C
Cuts at: 0 190 1726 3202 4335
Size: 190 1536 1476 1133
Fragments arranged by size:
1536 1476 1133 190
BanII G_rGCy'C
Cuts at: 0 111 481 1492 1691 1868 2519 3163 4335
Size: 111 370 1011 199 177 651 644 1172
Fragments arranged by size:
1172 1011 651 644 370 199 177 111
BbsI GAAGACnn'nnnn_
Cuts at: 0 323 798 1192 1421 2132 2414 2877 4335
Size: 323 475 394 229 711 282 463 1458
Fragments arranged by size:
1458 711 475 463 394 323 282 229
BbvCI CC'TCA_GC
Cuts at: 0 1626 3135 4335
Size: 1626 1509 1200
Fragments arranged by size:
1626 1509 1200
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1527 1561 4335
Size: 1527 34 2774
Fragments arranged by size:
2774 1527 34
BclI T'GATC_A
Cuts at: 0 2658 2883 4154 4335
Size: 2658 225 1271 181
Fragments arranged by size:
2658 1271 225 181
BglI GCCn_nnn'nGGC
Cuts at: 0 1408 1519 4335
Size: 1408 111 2816
Fragments arranged by size:
2816 1408 111
BlpI GC'TnA_GC
Cuts at: 0 471 4222 4335
Size: 471 3751 113
Fragments arranged by size:
3751 471 113
Bme1580I G_kGCm'C
Cuts at: 0 111 1379 1492 1691 1729 2030 3163 3176
Size: 111 1268 113 199 38 301 1133 13
Cuts at: 3176 4089 4335
Size: 913 246
Fragments arranged by size:
1268 1133 913 301 246 199 113 111
38 13
BmgBI CAC'GTC
Cuts at: 0 218 4335
Size: 218 4117
BmrI ACTGGGnnnn_n'
Cuts at: 0 186 219 462 1021 1490 4335
Size: 186 33 243 559 469 2845
Fragments arranged by size:
2845 559 469 243 186 33
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1821 4335
Size: 1821 2514
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1119 1482 1667 2177 2304 2826 4132 4193
Size: 1119 363 185 510 127 522 1306 61
Cuts at: 4193 4335
Size: 142
Fragments arranged by size:
1306 1119 522 510 363 185 142 127
61
Bpu10I CC'TnA_GC
Cuts at: 0 203 1626 3135 4335
Size: 203 1423 1509 1200
Fragments arranged by size:
1509 1423 1200 203
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 862 4335
Size: 862 3473
BsaI GGTCTCn'nnnn_
Cuts at: 0 682 807 4335
Size: 682 125 3528
Fragments arranged by size:
3528 682 125
BsaHI Gr'CG_yC
Cuts at: 0 1441 4335
Size: 1441 2894
BsaWI w'CCGG_w
Cuts at: 0 809 2463 4335
Size: 809 1654 1872
Fragments arranged by size:
1872 1654 809
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1132 1162 1174 1204 1371 1401 2277 2307
Size: 1132 30 12 30 167 30 876 30
Cuts at: 2307 4335
Size: 2028
Fragments arranged by size:
2028 1132 876 167 30 30 30 30
12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1485 1569 1801 1861 2059 2372 3141 3331
Size: 1485 84 232 60 198 313 769 190
Cuts at: 3331 4335
Size: 1004
Fragments arranged by size:
1485 1004 769 313 232 198 190 84
60
BseYI C'CCAG_C
Cuts at: 0 1588 1867 4103 4335
Size: 1588 279 2236 232
Fragments arranged by size:
2236 1588 279 232
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 160 620 1084 3379 4335
Size: 97 63 460 464 2295 956
Fragments arranged by size:
2295 956 464 460 97 63
BsiEI CG_ry'CG
Cuts at: 0 1622 4335
Size: 1622 2713
BsiHKAI G_wGCw'C
Cuts at: 0 62 209 1784 2276 2519 2947 3176 3510
Size: 62 147 1575 492 243 428 229 334
Cuts at: 3510 3570 4335
Size: 60 765
Fragments arranged by size:
1575 765 492 428 334 243 229 147
62 60
BsmI GAATG_Cn'
Cuts at: 0 2601 4335
Size: 2601 1734
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1391 1889 4335
Size: 1391 498 2446
Fragments arranged by size:
2446 1391 498
Bsp1286I G_dGCh'C
Cuts at: 0 62 111 209 481 1379 1492 1691 1729
Size: 62 49 98 272 898 113 199 38
Cuts at: 1729 1784 1868 2030 2276 2519 2947 3163 3176
Size: 55 84 162 246 243 428 216 13
Cuts at: 3176 3510 3570 4089 4335
Size: 334 60 519 246
Fragments arranged by size:
898 519 428 334 272 246 246 243
216 199 162 113 98 84 62 60
55 49 38 13
BspEI T'CCGG_A
Cuts at: 0 809 2463 4335
Size: 809 1654 1872
Fragments arranged by size:
1872 1654 809
BspHI T'CATG_A
Cuts at: 0 678 1133 4047 4335
Size: 678 455 2914 288
Fragments arranged by size:
2914 678 455 288
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1080 4335
Size: 1080 3255
BsrBI CCG'CTC
Cuts at: 0 1776 1851 4335
Size: 1776 75 2484
Fragments arranged by size:
2484 1776 75
BsrDI GCAATG_nn'
Cuts at: 0 471 2264 2762 3586 3657 4223 4335
Size: 471 1793 498 824 71 566 112
Fragments arranged by size:
1793 824 566 498 471 112 71
BsrFI r'CCGG_y
Cuts at: 0 1549 1984 4335
Size: 1549 435 2351
Fragments arranged by size:
2351 1549 435
BssSI C'ACGA_G
Cuts at: 0 115 1783 4335
Size: 115 1668 2552
Fragments arranged by size:
2552 1668 115
BstAPI GCAn_nnn'nTGC
Cuts at: 0 933 3678 4335
Size: 933 2745 657
Fragments arranged by size:
2745 933 657
BstEII G'GTnAC_C
Cuts at: 0 1530 1830 2010 4335
Size: 1530 300 180 2325
Fragments arranged by size:
2325 1530 300 180
BstXI CCAn_nnnn'nTGG
Cuts at: 0 466 4335
Size: 466 3869
BstYI r'GATC_y
Cuts at: 0 143 642 699 3130 4335
Size: 143 499 57 2431 1205
Fragments arranged by size:
2431 1205 499 143 57
BtgI C'CryG_G
Cuts at: 0 596 654 866 1472 2106 4335
Size: 596 58 212 606 634 2229
Fragments arranged by size:
2229 634 606 596 212 58
BtsI GCAGTG_nn'
Cuts at: 0 143 1084 1943 2591 3174 3186 4335
Size: 143 941 859 648 583 12 1149
Fragments arranged by size:
1149 941 859 648 583 143 12
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3473 3508 4335
Size: 3473 35 827
Fragments arranged by size:
3473 827 35
DraIII CAC_nnn'GTG
Cuts at: 0 1598 3476 4335
Size: 1598 1878 859
Fragments arranged by size:
1878 1598 859
DrdI GACnn_nn'nnGTC
Cuts at: 0 397 4335
Size: 397 3938
EaeI y'GGCC_r
Cuts at: 0 599 1619 2893 3787 4335
Size: 599 1020 1274 894 548
Fragments arranged by size:
1274 1020 894 599 548
EagI C'GGCC_G
Cuts at: 0 1619 4335
Size: 1619 2716
EarI CTCTTCn'nnn_
Cuts at: 0 1401 1816 2935 3549 4007 4335
Size: 1401 415 1119 614 458 328
Fragments arranged by size:
1401 1119 614 458 415 328
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 948 1985 4335
Size: 948 1037 2350
Fragments arranged by size:
2350 1037 948
EcoICRI GAG'CTC
Cuts at: 0 2517 4335
Size: 2517 1818
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 146 1119 1382 1482 1667 2144 2177 2304
Size: 146 973 263 100 185 477 33 127
Cuts at: 2304 2826 2959 3295 3395 3530 4132 4193 4335
Size: 522 133 336 100 135 602 61 142
Fragments arranged by size:
973 602 522 477 336 263 185 146
142 135 133 127 100 100 61 33
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2192 2687 4023 4335
Size: 2192 495 1336 312
Fragments arranged by size:
2192 1336 495 312
EcoO109I rG'GnC_Cy
Cuts at: 0 107 108 717 1489 1687 2776 3160 4335
Size: 107 1 609 772 198 1089 384 1175
Fragments arranged by size:
1175 1089 772 609 384 198 107 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 708 3376 4237 4335
Size: 708 2668 861 98
Fragments arranged by size:
2668 861 708 98
FspI TGC'GCA
Cuts at: 0 1294 4335
Size: 1294 3041
HaeII r_GCGC'y
Cuts at: 0 2571 4335
Size: 2571 1764
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 313 345 2202 2234 3045 3077 3702 3734
Size: 313 32 1857 32 811 32 625 32
Cuts at: 3734 3842 3874 4335
Size: 108 32 461
Fragments arranged by size:
1857 811 625 461 313 108 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 89 3258 3408 4335
Size: 89 3169 150 927
Fragments arranged by size:
3169 927 150 89
HpaI GTT'AAC
Cuts at: 0 3258 3408 4335
Size: 3258 150 927
Fragments arranged by size:
3258 927 150
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1261 3329 4335
Size: 1261 2068 1006
Fragments arranged by size:
2068 1261 1006
MscI TGG'CCA
Cuts at: 0 601 2895 3789 4335
Size: 601 2294 894 546
Fragments arranged by size:
2294 894 601 546
MslI CAynn'nnrTG
Cuts at: 0 464 777 1054 1060 2023 2269 2399 2510
Size: 464 313 277 6 963 246 130 111
Cuts at: 2510 2842 3179 3401 3739 4335
Size: 332 337 222 338 596
Fragments arranged by size:
963 596 464 338 337 332 313 277
246 222 130 111 6
MspA1I CmG'CkG
Cuts at: 0 230 475 1157 2108 2155 3393 4335
Size: 230 245 682 951 47 1238 942
Fragments arranged by size:
1238 951 942 682 245 230 47
NaeI GCC'GGC
Cuts at: 0 1551 1986 4335
Size: 1551 435 2349
Fragments arranged by size:
2349 1551 435
NcoI C'CATG_G
Cuts at: 0 596 1472 4335
Size: 596 876 2863
Fragments arranged by size:
2863 876 596
NdeI CA'TA_TG
Cuts at: 0 2655 4335
Size: 2655 1680
NgoMIV G'CCGG_C
Cuts at: 0 1549 1984 4335
Size: 1549 435 2351
Fragments arranged by size:
2351 1549 435
NspI r_CATG'y
Cuts at: 0 1293 2959 3228 4335
Size: 1293 1666 269 1107
Fragments arranged by size:
1666 1293 1107 269
PasI CC'CwG_GG
Cuts at: 0 735 4335
Size: 735 3600
PflMI CCAn_nnn'nTGG
Cuts at: 0 3592 3762 4110 4335
Size: 3592 170 348 225
Fragments arranged by size:
3592 348 225 170
PfoI T'CCnGG_A
Cuts at: 0 713 973 1096 4335
Size: 713 260 123 3239
Fragments arranged by size:
3239 713 260 123
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 293 325 3526 3558 4335
Size: 293 32 3201 32 777
Fragments arranged by size:
3201 777 293 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 717 2776 4335
Size: 717 2059 1559
Fragments arranged by size:
2059 1559 717
PsiI TTA'TAA
Cuts at: 0 3664 4335
Size: 3664 671
PspOMI G'GGCC_C
Cuts at: 0 107 1488 1687 3159 4335
Size: 107 1381 199 1472 1176
Fragments arranged by size:
1472 1381 1176 199 107
PspXI vC'TCGA_Gb
Cuts at: 0 2423 3569 4335
Size: 2423 1146 766
Fragments arranged by size:
2423 1146 766
PstI C_TGCA'G
Cuts at: 0 1091 2598 3181 4335
Size: 1091 1507 583 1154
Fragments arranged by size:
1507 1154 1091 583
PvuII CAG'CTG
Cuts at: 0 230 1157 3393 4335
Size: 230 927 2236 942
Fragments arranged by size:
2236 942 927 230
SacI G_AGCT'C
Cuts at: 0 2519 4335
Size: 2519 1816
SacII CC_GC'GG
Cuts at: 0 2109 4335
Size: 2109 2226
SapI GCTCTTCn'nnn_
Cuts at: 0 2935 4335
Size: 2935 1400
ScaI AGT'ACT
Cuts at: 0 3113 4335
Size: 3113 1222
SfcI C'TryA_G
Cuts at: 0 1087 1152 2594 3177 4335
Size: 1087 65 1442 583 1158
Fragments arranged by size:
1442 1158 1087 583 65
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1519 4335
Size: 1519 2816
SmlI C'TyrA_G
Cuts at: 0 841 2423 3569 4335
Size: 841 1582 1146 766
Fragments arranged by size:
1582 1146 841 766
SphI G_CATG'C
Cuts at: 0 3228 4335
Size: 3228 1107
SspI AAT'ATT
Cuts at: 0 1343 4335
Size: 1343 2992
StuI AGG'CCT
Cuts at: 0 1672 1994 3820 4018 4170 4335
Size: 1672 322 1826 198 152 165
Fragments arranged by size:
1826 1672 322 198 165 152
StyI C'CwwG_G
Cuts at: 0 596 1347 1472 1673 1712 2309 2772 3026
Size: 596 751 125 201 39 597 463 254
Cuts at: 3026 4335
Size: 1309
Fragments arranged by size:
1309 751 597 596 463 254 201 125
39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1062 1139 1143 2035 2129 2785 4335
Size: 1062 77 4 892 94 656 1550
Fragments arranged by size:
1550 1062 892 656 94 77 4
TatI w'GTAC_w
Cuts at: 0 2572 3111 3323 3782 4335
Size: 2572 539 212 459 553
Fragments arranged by size:
2572 553 539 459 212
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1371 1403 2133 2165 2825 2857 4335
Size: 1371 32 730 32 660 32 1478
Fragments arranged by size:
1478 1371 730 660 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 883 2008 3919 4335
Size: 883 1125 1911 416
Fragments arranged by size:
1911 1125 883 416
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1671 4335
Size: 1671 2664
XhoI C'TCGA_G
Cuts at: 0 2423 3569 4335
Size: 2423 1146 766
Fragments arranged by size:
2423 1146 766
XmnI GAAnn'nnTTC
Cuts at: 0 2444 2480 3356 3543 3697 4335
Size: 2444 36 876 187 154 638
Fragments arranged by size:
2444 876 638 187 154 36
ZraI GAC'GTC
Cuts at: 0 1442 4335
Size: 1442 2893
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AhdI AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AvaI BaeI BamHI
BanI BanII BbsI BbvCI BcgI BclI BglI BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstEII BstXI BstYI BtgI BtsI
CspCI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I HincII
HpaI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NspI PasI PflMI PfoI PpiI PpuMI PsiI
PspOMI PspXI PstI PvuII SacI SacII SapI ScaI
SfcI SfiI SmlI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XcmI XhoI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AflII AflIII AgeI AloI AscI AsiSI
AvrII BbeI BciVI BfrBI BglII BmtI BsaAI BsaBI
BsiWI BsrGI BssHII BstBI BstZ17I Bsu36I BtgZI ClaI
DraI EcoRI EcoRV FseI FspAI HindIII KasI KpnI
MfeI MluI NarI NheI NotI NruI NsiI PacI
PciI PmeI PmlI PshAI PsrI PvuI RsrII SalI
SanDI SbfI SexAI SfoI SgfI SgrAI SmaI SnaBI
SpeI SrfI SwaI XbaI XmaI