(Circular) (Six-base) MAPSORT of: pFN21AB4514.seq Check: 1125 from: 1 to: 9565 pFN21AB4514 9565 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3075 4625 5103 3075 Size: 1550 478 7537 Fragments arranged by size: 7537 1550 478 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7375 278 Size: 53 83 186 936 5839 2468 Fragments arranged by size: 5839 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2202 4980 6078 6908 1937 Size: 265 2778 1098 830 4594 Fragments arranged by size: 4594 2778 1098 830 265 Acc65I G'GTAC_C Cuts at: 6709 6886 6709 Size: 177 9388 AclI AA'CG_TT Cuts at: 1563 6636 7692 8065 1563 Size: 5073 1056 373 3063 Fragments arranged by size: 5073 3063 1056 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2264 2405 3290 5348 5484 6135 7628 9012 Size: 632 141 885 2058 136 651 1493 1384 Cuts at: 9012 9280 1632 Size: 268 1917 Fragments arranged by size: 2058 1917 1493 1384 885 651 632 268 141 136 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 9368 AflIII A'CryG_T Cuts at: 1203 3693 4064 4566 6427 8464 9441 1203 Size: 2490 371 502 1861 2037 977 1327 Fragments arranged by size: 2490 2037 1861 1327 977 502 371 AgeI A'CCGG_T Cuts at: 8396 8396 Size: 9565 AhdI GACnn_n'nnGTC Cuts at: 5617 5941 8294 5617 Size: 324 2353 6888 Fragments arranged by size: 6888 2353 324 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 9565 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4716 1184 Size: 3532 6033 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2326 2358 3518 3550 5478 5510 2326 Size: 32 1160 32 1928 32 6381 Fragments arranged by size: 6381 1928 1160 32 32 32 AlwNI CAG_nnn'CTG Cuts at: 3100 3825 4971 5200 5766 5789 7308 8880 3100 Size: 725 1146 229 566 23 1519 1572 3785 Fragments arranged by size: 3785 1572 1519 1146 725 566 229 23 ApaI G_GGCC'C Cuts at: 2475 4083 4160 6864 2475 Size: 1608 77 2704 5176 Fragments arranged by size: 5176 2704 1608 77 ApaLI G'TGCA_C Cuts at: 3056 7622 8778 3056 Size: 4566 1156 3843 Fragments arranged by size: 4566 3843 1156 ApoI r'AATT_y Cuts at: 1493 4009 5522 6874 7100 7252 9361 1493 Size: 2516 1513 1352 226 152 2109 1697 Fragments arranged by size: 2516 2109 1697 1513 1352 226 152 AseI AT'TA_AT Cuts at: 160 1032 8119 160 Size: 872 7087 1606 Fragments arranged by size: 7087 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9565 AvaI C'yCGr_G Cuts at: 1870 1943 2124 2208 2258 2477 2576 4001 4213 Size: 73 181 84 50 219 99 1425 212 Cuts at: 4213 5647 5694 5826 6890 1870 Size: 1434 47 132 1064 4545 Fragments arranged by size: 4545 1434 1425 1064 219 212 181 132 99 84 73 50 47 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 6701 6734 6701 Size: 33 9532 BamHI G'GATC_C Cuts at: 2562 3857 6485 6895 7262 2562 Size: 1295 2628 410 367 4865 Fragments arranged by size: 4865 2628 1295 410 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2182 2317 2874 3063 4186 Size: 325 217 627 395 135 557 189 1123 Cuts at: 4186 4510 4700 5335 6709 6886 8341 618 Size: 324 190 635 1374 177 1455 1842 Fragments arranged by size: 1842 1455 1374 1123 635 627 557 395 325 324 217 190 189 177 135 BanII G_rGCy'C Cuts at: 1391 2022 2066 2096 2475 3323 3641 3654 4083 Size: 631 44 30 379 848 318 13 429 Cuts at: 4083 4160 5231 5598 6681 6864 6884 1391 Size: 77 1071 367 1083 183 20 4072 Fragments arranged by size: 4072 1083 1071 848 631 429 379 367 318 183 77 44 30 20 13 BbeI G_GCGC'C Cuts at: 2321 2878 2321 Size: 557 9008 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 5459 6123 928 Size: 971 3560 664 4370 Fragments arranged by size: 4370 3560 971 664 BbvCI CC'TCA_GC Cuts at: 2980 5049 2980 Size: 2069 7496 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5675 5709 7756 7790 1903 Size: 34 3738 34 2047 34 3678 Fragments arranged by size: 3738 3678 2047 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6814 7457 8667 6814 Size: 643 1210 7712 Fragments arranged by size: 7712 1210 643 BclI T'GATC_A Cuts at: 1548 4518 5511 1548 Size: 2970 993 5602 Fragments arranged by size: 5602 2970 993 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3872 4553 5138 Size: 107 122 71 631 605 2200 681 585 Cuts at: 5138 6244 8176 136 Size: 1106 1932 1525 Fragments arranged by size: 2200 1932 1525 1106 681 631 605 585 122 107 71 BglII A'GATC_T Cuts at: 2658 4176 9560 2658 Size: 1518 5384 2663 Fragments arranged by size: 5384 2663 1518 BlpI GC'TnA_GC Cuts at: 5243 6982 5243 Size: 1739 7826 Bme1580I G_kGCm'C Cuts at: 1790 2187 2475 3060 3391 4083 4160 4515 6864 Size: 397 288 585 331 692 77 355 2349 Cuts at: 6864 7626 8782 1790 Size: 762 1156 2573 Fragments arranged by size: 2573 2349 1156 762 692 585 397 355 331 288 77 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 9565 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4350 4898 6654 Size: 416 53 83 399 24 2933 548 1756 Cuts at: 6654 7296 8254 442 Size: 642 958 1753 Fragments arranged by size: 2933 1756 1753 958 642 548 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2279 1056 Size: 1223 8342 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2914 3146 4612 2914 Size: 232 1466 7867 Fragments arranged by size: 7867 1466 232 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2453 2486 2816 2846 3129 3155 3211 3335 Size: 1214 33 330 30 283 26 56 124 Cuts at: 3335 4319 4607 5612 6041 6629 6822 8225 1239 Size: 984 288 1005 429 588 193 1403 2579 Fragments arranged by size: 2579 1403 1214 1005 984 588 429 330 288 283 193 124 56 33 30 26 Bpu10I CC'TnA_GC Cuts at: 2300 2980 4133 5049 2300 Size: 680 1153 916 6816 Fragments arranged by size: 6816 1153 916 680 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3703 3793 5600 7690 8555 8853 3703 Size: 90 1807 2090 865 298 4415 Fragments arranged by size: 4415 2090 1807 865 298 90 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2332 5604 5627 6286 6391 8228 882 Size: 617 833 3272 23 659 105 1837 2219 Fragments arranged by size: 3272 2219 1837 833 659 617 105 23 BsaAI yAC'GTr Cuts at: 493 1204 4483 5689 6430 7356 493 Size: 711 3279 1206 741 926 2702 Fragments arranged by size: 3279 2702 1206 926 741 711 BsaBI GATnn'nnATC Cuts at: 9559 9559 Size: 9565 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2318 2588 2875 3187 Size: 53 83 186 936 785 270 287 312 Cuts at: 3187 4347 5058 7372 7754 8389 275 Size: 1160 711 2314 382 635 1451 Fragments arranged by size: 2314 1451 1160 936 785 711 635 382 312 287 270 186 83 53 BsaWI w'CCGG_w Cuts at: 4625 4706 5411 6081 7998 8396 8670 8817 9228 Size: 81 705 670 1917 398 274 147 411 Cuts at: 9228 4625 Size: 4962 Fragments arranged by size: 4962 1917 705 670 411 398 274 147 81 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3013 3043 3872 3902 4109 Size: 30 165 30 1610 30 829 30 207 Cuts at: 4109 4139 4494 4524 1178 Size: 30 355 30 6219 Fragments arranged by size: 6219 1610 829 355 207 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2224 2459 2906 3034 3489 3794 4473 4476 Size: 1038 235 447 128 455 305 679 3 Cuts at: 4476 4588 4780 4815 5302 5650 6261 6317 6320 Size: 112 192 35 487 348 611 56 3 Cuts at: 6320 6337 1186 Size: 17 4414 Fragments arranged by size: 4414 1038 679 611 487 455 447 348 305 235 192 128 112 56 35 17 3 3 BseYI C'CCAG_C Cuts at: 2098 2308 2437 2637 3093 4282 4906 5996 6316 Size: 210 129 200 456 1189 624 1090 320 Cuts at: 6316 8768 9264 2098 Size: 2452 496 2399 Fragments arranged by size: 2452 2399 1189 1090 624 496 456 320 210 200 129 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2821 3022 3609 5669 6145 1163 Size: 573 1085 201 587 2060 476 4583 Fragments arranged by size: 4583 2060 1085 587 573 476 201 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6414 7015 7776 8804 1540 Size: 275 186 4413 601 761 1028 2301 Fragments arranged by size: 4413 2301 1028 761 601 275 186 BsiHKAI G_wGCw'C Cuts at: 3036 3060 3171 3323 3641 3654 4790 5474 5598 Size: 24 111 152 318 13 1136 684 124 Cuts at: 5598 7626 7711 8782 3036 Size: 2028 85 1071 3819 Fragments arranged by size: 3819 2028 1136 1071 684 318 152 124 111 85 24 13 BsiWI C'GTAC_G Cuts at: 6522 6522 Size: 9565 BsmI GAATG_Cn' Cuts at: 5421 6440 7076 7169 5421 Size: 1019 636 93 7817 Fragments arranged by size: 7817 1019 636 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3055 5065 3055 Size: 2010 7555 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2022 2066 2096 2187 2475 3036 3060 Size: 399 232 44 30 91 288 561 24 Cuts at: 3060 3171 3323 3391 3641 3654 4083 4160 4515 Size: 111 152 68 250 13 429 77 355 Cuts at: 4515 4790 5231 5474 5598 6681 6864 6884 7626 Size: 275 441 243 124 1083 183 20 742 Cuts at: 7626 7711 8782 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1083 1071 742 561 441 429 399 355 288 275 250 243 232 183 152 124 111 91 85 77 68 44 30 24 20 13 BspEI T'CCGG_A Cuts at: 4625 5411 9228 4625 Size: 786 3817 4962 Fragments arranged by size: 4962 3817 786 BspHI T'CATG_A Cuts at: 5169 5268 7454 9184 5169 Size: 99 2186 1730 5550 Fragments arranged by size: 5550 2186 1730 99 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3075 3499 4625 5103 6920 844 Size: 2231 424 1126 478 1817 3489 Fragments arranged by size: 3489 2231 1817 1126 478 424 BsrBI CCG'CTC Cuts at: 1394 1823 7452 1394 Size: 429 5629 3507 Fragments arranged by size: 5629 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3087 4521 4831 5700 5751 8060 8234 Size: 1183 1839 1434 310 869 51 2309 174 Cuts at: 8234 65 Size: 1396 Fragments arranged by size: 2309 1839 1434 1396 1183 869 310 174 51 BsrFI r'CCGG_y Cuts at: 1700 1810 2078 2314 2339 4507 6409 8209 8396 Size: 110 268 236 25 2168 1902 1800 187 Cuts at: 8396 1700 Size: 2869 Fragments arranged by size: 2869 2168 1902 1800 268 236 187 110 25 BsrGI T'GTAC_A Cuts at: 96 4317 4899 96 Size: 4221 582 4762 Fragments arranged by size: 4762 4221 582 BssHII G'CGCG_C Cuts at: 1925 2086 1925 Size: 161 9404 BssSI C'ACGA_G Cuts at: 5135 6587 7625 8637 5135 Size: 1452 1038 1012 6063 Fragments arranged by size: 6063 1452 1038 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2428 5209 5572 794 Size: 1634 2781 363 4787 Fragments arranged by size: 4787 2781 1634 363 BstBI TT'CG_AA Cuts at: 8369 8369 Size: 9565 BstEII G'GTnAC_C Cuts at: 1184 5122 6596 1184 Size: 3938 1474 4153 Fragments arranged by size: 4153 3938 1474 BstXI CCAn_nnnn'nTGG Cuts at: 2425 3898 5215 5886 6142 2425 Size: 1473 1317 671 256 5848 Fragments arranged by size: 5848 1473 1317 671 256 BstYI r'GATC_y Cuts at: 1972 2562 2658 3126 3857 3933 4176 6485 6895 Size: 590 96 468 731 76 243 2309 410 Cuts at: 6895 7262 7647 7664 9116 9202 9214 9560 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 2309 1977 1452 731 590 468 410 385 367 346 243 96 86 76 17 12 BstZ17I GTA'TAC Cuts at: 6079 6079 Size: 9565 Bsu36I CC'TnA_GG Cuts at: 2852 4792 6258 2852 Size: 1940 1466 6159 Fragments arranged by size: 6159 1940 1466 BtgI C'CryG_G Cuts at: 513 1065 2004 2049 2266 2619 3011 4084 4267 Size: 552 939 45 217 353 392 1073 183 Cuts at: 4267 4930 5032 5339 5374 6414 6481 513 Size: 663 102 307 35 1040 67 3597 Fragments arranged by size: 3597 1073 1040 939 663 552 392 353 307 217 183 102 67 45 35 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2762 487 Size: 683 56 1536 7290 Fragments arranged by size: 7290 1536 683 56 BtsI GCAGTG_nn' Cuts at: 2840 3034 3258 3286 3791 6665 7081 7875 7895 Size: 194 224 28 505 2874 416 794 20 Cuts at: 7895 2840 Size: 4510 Fragments arranged by size: 4510 2874 794 505 416 224 194 28 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9565 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9530 DraI TTT'AAA Cuts at: 4914 6869 4914 Size: 1955 7610 DraIII CAC_nnn'GTG Cuts at: 4273 4273 Size: 9565 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 8572 809 Size: 507 7256 1802 Fragments arranged by size: 7256 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2080 3045 5139 Size: 54 1425 50 275 18 250 965 2094 Cuts at: 5139 5667 5799 6411 7012 7901 9530 8 Size: 528 132 612 601 889 1629 43 Fragments arranged by size: 2094 1629 1425 965 889 612 601 528 275 250 132 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6411 7012 1537 Size: 275 4599 601 4090 Fragments arranged by size: 4599 4090 601 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2153 2198 2657 5788 6216 6222 6688 7495 Size: 800 45 459 3131 428 6 466 807 Cuts at: 7495 1353 Size: 3423 Fragments arranged by size: 3423 3131 807 800 466 459 428 45 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2555 4470 4821 8152 8526 8672 Size: 1064 138 966 1915 351 3331 374 146 Cuts at: 8672 387 Size: 1280 Fragments arranged by size: 3331 1915 1280 1064 966 374 351 146 138 EcoICRI GAG'CTC Cuts at: 3321 3639 3652 5596 3321 Size: 318 13 1944 7290 Fragments arranged by size: 7290 1944 318 13 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2264 2405 2453 2486 2816 2846 3129 Size: 393 632 141 48 33 330 30 283 Cuts at: 3129 3155 3211 3290 3335 4319 4607 5348 5484 Size: 26 56 79 45 984 288 741 136 Cuts at: 5484 5612 6041 6135 6629 6822 7628 8225 9012 Size: 128 429 94 494 193 806 597 787 Cuts at: 9012 9280 1239 Size: 268 1524 Fragments arranged by size: 1524 984 806 787 741 632 597 494 429 393 330 288 283 268 193 141 136 128 94 79 56 48 45 33 30 26 EcoNI CCTnn'n_nnAGG Cuts at: 3079 4301 5330 3079 Size: 1222 1029 7314 Fragments arranged by size: 7314 1222 1029 EcoO109I rG'GnC_Cy Cuts at: 2212 2472 3516 4080 4156 4157 4545 5786 6169 Size: 260 1044 564 76 1 388 1241 383 Cuts at: 6169 6253 6660 2212 Size: 84 407 5117 Fragments arranged by size: 5117 1241 1044 564 407 388 383 260 84 76 1 EcoRI G'AATT_C Cuts at: 6874 7252 6874 Size: 378 9187 FseI GG_CCGG'CC Cuts at: 6413 6413 Size: 9565 FspI TGC'GCA Cuts at: 2402 8071 2402 Size: 5669 3896 HaeII r_GCGC'y Cuts at: 1678 2321 2878 8712 1678 Size: 643 557 5834 2531 Fragments arranged by size: 5834 2531 643 557 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2146 2178 2547 2579 3127 Size: 32 135 32 574 32 369 32 548 Cuts at: 3127 3159 3872 3904 5045 5077 5603 5626 5635 Size: 32 713 32 1141 32 526 23 9 Cuts at: 5635 5658 5927 5959 6076 6108 8206 8238 8280 Size: 23 269 32 117 32 2098 32 42 Cuts at: 8280 8312 1373 Size: 32 2626 Fragments arranged by size: 2626 2098 1141 713 574 548 526 369 269 135 117 42 32 32 32 32 32 32 32 32 32 32 32 23 23 9 HincII GTy'rAC Cuts at: 669 1657 1938 2173 6909 7155 669 Size: 988 281 235 4736 246 3079 Fragments arranged by size: 4736 3079 988 281 246 235 HindIII A'AGCT_T Cuts at: 748 748 Size: 9565 HpaI GTT'AAC Cuts at: 7155 7155 Size: 9565 KasI G'GCGC_C Cuts at: 2317 2874 2317 Size: 557 9008 KpnI G_GTAC'C Cuts at: 6713 6890 6713 Size: 177 9388 MfeI C'AATT_G Cuts at: 7164 7164 Size: 9565 MluI A'CGCG_T Cuts at: 9441 9441 Size: 9565 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2571 3140 3314 4152 6477 8679 8863 1305 Size: 1266 569 174 838 2325 2202 184 2007 Fragments arranged by size: 2325 2202 2007 1266 838 569 184 174 MscI TGG'CCA Cuts at: 10 64 1489 1832 5669 5801 10 Size: 54 1425 343 3837 132 3774 Fragments arranged by size: 3837 3774 1425 343 132 54 MslI CAynn'nnrTG Cuts at: 518 1166 4256 5174 5213 5555 7523 7882 8041 Size: 648 3090 918 39 342 1968 359 159 Cuts at: 8041 518 Size: 2042 Fragments arranged by size: 3090 2042 1968 918 648 359 342 159 39 MspA1I CmG'CkG Cuts at: 1606 2038 2057 2102 2116 2268 2296 2621 2984 Size: 432 19 45 14 152 28 325 363 Cuts at: 2984 3097 4077 5341 6320 6377 6981 7658 8806 Size: 113 980 1264 979 57 604 677 1148 Cuts at: 8806 9051 1606 Size: 245 2120 Fragments arranged by size: 2120 1264 1148 980 979 677 604 432 363 325 245 152 113 57 45 28 19 14 NaeI GCC'GGC Cuts at: 2316 6411 2316 Size: 4095 5470 NarI GG'CG_CC Cuts at: 2318 2875 2318 Size: 557 9008 NcoI C'CATG_G Cuts at: 513 1065 2004 4084 5374 513 Size: 552 939 2080 1290 4704 Fragments arranged by size: 4704 2080 1290 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 9565 NgoMIV G'CCGG_C Cuts at: 2314 6409 2314 Size: 4095 5470 NheI G'CTAG_C Cuts at: 1052 2275 1052 Size: 1223 8342 NotI GC'GGCC_GC Cuts at: 7012 7012 Size: 9565 NspI r_CATG'y Cuts at: 1132 1229 2704 3755 4068 4315 6923 8468 1132 Size: 97 1475 1051 313 247 2608 1545 2229 Fragments arranged by size: 2608 2229 1545 1475 1051 313 247 97 PasI CC'CwG_GG Cuts at: 1348 3391 6068 1348 Size: 2043 2677 4845 Fragments arranged by size: 4845 2677 2043 PciI A'CATG_T Cuts at: 4064 8464 4064 Size: 4400 5165 PflMI CCAn_nnn'nTGG Cuts at: 1108 5789 6510 1108 Size: 4681 721 4163 Fragments arranged by size: 4681 4163 721 PfoI T'CCnGG_A Cuts at: 2066 2330 3537 2066 Size: 264 1207 8094 Fragments arranged by size: 8094 1207 264 PmeI GTTT'AAAC Cuts at: 6869 6869 Size: 9565 PmlI CAC'GTG Cuts at: 5689 6430 5689 Size: 741 8824 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7634 7666 9147 9179 7634 Size: 32 1481 32 8020 Fragments arranged by size: 8020 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2212 3516 6169 6253 2212 Size: 1304 2653 84 5524 Fragments arranged by size: 5524 2653 1304 84 PshAI GACnn'nnGTC Cuts at: 1613 4986 1613 Size: 3373 6192 PsiI TTA'TAA Cuts at: 7135 7135 Size: 9565 PspOMI G'GGCC_C Cuts at: 2471 4079 4156 6860 2471 Size: 1608 77 2704 5176 Fragments arranged by size: 5176 2704 1608 77 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 6506 6538 6506 Size: 32 9533 PstI C_TGCA'G Cuts at: 830 2121 2154 3072 3253 3281 3318 3834 3942 Size: 1291 33 918 181 28 37 516 108 Cuts at: 3942 4967 5226 6382 6917 830 Size: 1025 259 1156 535 3478 Fragments arranged by size: 3478 1291 1156 1025 918 535 516 259 181 108 37 33 28 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9565 PvuII CAG'CTG Cuts at: 2057 2102 2984 3097 6320 6377 2057 Size: 45 882 113 3223 57 5245 Fragments arranged by size: 5245 3223 882 113 57 45 SacI G_AGCT'C Cuts at: 3323 3641 3654 5598 3323 Size: 318 13 1944 7290 Fragments arranged by size: 7290 1944 318 13 SacII CC_GC'GG Cuts at: 2269 2622 5342 2269 Size: 353 2720 6492 Fragments arranged by size: 6492 2720 353 SalI G'TCGA_C Cuts at: 1936 6907 1936 Size: 4971 4594 SapI GCTCTTCn'nnn_ Cuts at: 2198 2198 Size: 9565 SbfI CC_TGCA'GG Cuts at: 6917 6917 Size: 9565 ScaI AGT'ACT Cuts at: 7813 9525 7813 Size: 1712 7853 SfcI C'TryA_G Cuts at: 826 1045 2117 2150 3068 3249 3277 3314 3600 Size: 219 1072 33 918 181 28 37 286 Cuts at: 3600 3830 3938 4041 4204 4963 5222 5836 5851 Size: 230 108 103 163 759 259 614 15 Cuts at: 5851 6378 6567 6913 8048 8729 8920 826 Size: 527 189 346 1135 681 191 1471 Fragments arranged by size: 1471 1135 1072 918 759 681 614 527 346 286 259 230 219 191 189 181 163 108 103 37 33 28 15 SfiI GGCCn_nnn'nGGCC Cuts at: 4553 4553 Size: 9565 SfoI GGC'GCC Cuts at: 2319 2876 2319 Size: 557 9008 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9565 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9565 SmaI CCC'GGG Cuts at: 1872 2126 2210 2479 4003 6892 1872 Size: 254 84 269 1524 2889 4545 Fragments arranged by size: 4545 2889 1524 269 254 84 SmlI C'TyrA_G Cuts at: 820 1017 1943 3682 3772 5615 7669 8570 8832 Size: 197 926 1739 90 1843 2054 901 262 Cuts at: 8832 820 Size: 1553 Fragments arranged by size: 2054 1843 1739 1553 926 901 262 197 90 SnaBI TAC'GTA Cuts at: 493 4483 7356 493 Size: 3990 2873 2702 Fragments arranged by size: 3990 2873 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 9565 SphI G_CATG'C Cuts at: 1132 6923 1132 Size: 5791 3774 SspI AAT'ATT Cuts at: 5 52 4945 5919 7489 5 Size: 47 4893 974 1570 2081 Fragments arranged by size: 4893 2081 1570 974 47 StuI AGG'CCT Cuts at: 1517 2897 3365 5903 1517 Size: 1380 468 2538 5179 Fragments arranged by size: 5179 2538 1380 468 StyI C'CwwG_G Cuts at: 513 1065 2004 2542 2682 3475 3699 4084 5374 Size: 552 939 538 140 793 224 385 1290 Cuts at: 5374 6249 7004 513 Size: 875 755 3074 Fragments arranged by size: 3074 1290 939 875 793 755 552 538 385 224 140 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3967 4109 4590 5092 5288 6070 6179 Size: 1255 2387 142 481 502 196 782 109 Cuts at: 6179 7592 7609 7762 7947 8392 325 Size: 1413 17 153 185 445 1498 Fragments arranged by size: 2387 1498 1413 1255 782 502 481 445 196 185 153 142 109 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4317 4358 4899 Size: 274 80 33 51 1443 2340 41 541 Cuts at: 4899 5235 6620 7811 9523 96 Size: 336 1385 1191 1712 138 Fragments arranged by size: 2340 1712 1443 1385 1191 541 336 274 138 80 51 41 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3013 3045 4494 4526 4878 4910 9525 9557 3013 Size: 32 1449 32 352 32 4615 32 3021 Fragments arranged by size: 4615 3021 1449 352 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2371 1316 Size: 1055 8510 XbaI T'CTAG_A Cuts at: 6901 6901 Size: 9565 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2056 3868 4135 5381 2056 Size: 1812 267 1246 6240 Fragments arranged by size: 6240 1812 1246 267 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 9565 XmaI C'CCGG_G Cuts at: 1870 2124 2208 2477 4001 6890 1870 Size: 254 84 269 1524 2889 4545 Fragments arranged by size: 4545 2889 1524 269 254 84 XmnI GAAnn'nnTTC Cuts at: 7694 7694 Size: 9565 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7373 276 Size: 53 83 186 936 5839 2468 Fragments arranged by size: 5839 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BfrBI EcoRV FalI FspAI NruI NsiI PacI PspXI RsrII SanDI SexAI SrfI SwaI