(Circular) (Six-base) MAPSORT of: pFN21AB4393.seq Check: 7127 from: 1 to: 8875 pFN21AB4393 8875 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 5162 5162 Size: 8875 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6685 278 Size: 53 83 186 936 5149 2468 Fragments arranged by size: 5149 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4071 4581 6218 1937 Size: 2134 510 1637 4594 Fragments arranged by size: 4594 2134 1637 510 Acc65I G'GTAC_C Cuts at: 2495 6196 2495 Size: 3701 5174 AclI AA'CG_TT Cuts at: 1563 7002 7375 1563 Size: 5439 373 3063 Fragments arranged by size: 5439 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2281 2399 2570 2721 3059 3445 3790 4154 Size: 649 118 171 151 338 386 345 364 Cuts at: 4154 4283 5174 6938 8322 8590 1632 Size: 129 891 1764 1384 268 1917 Fragments arranged by size: 1917 1764 1384 891 649 386 364 345 338 268 171 151 129 118 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8678 AflIII A'CryG_T Cuts at: 1203 7774 8751 1203 Size: 6571 977 1327 Fragments arranged by size: 6571 1327 977 AgeI A'CCGG_T Cuts at: 7706 7706 Size: 8875 AhdI GACnn_n'nnGTC Cuts at: 7604 7604 Size: 8875 AleI CACnn'nnGTG Cuts at: 1166 5828 1166 Size: 4662 4213 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8875 AlwNI CAG_nnn'CTG Cuts at: 2380 4302 5096 5578 6618 8190 2380 Size: 1922 794 482 1040 1572 3065 Fragments arranged by size: 3065 1922 1572 1040 794 482 ApaLI G'TGCA_C Cuts at: 6932 8088 6932 Size: 1156 7719 ApoI r'AATT_y Cuts at: 1493 2578 2677 3168 4916 6152 6184 6410 6562 Size: 1085 99 491 1748 1236 32 226 152 Cuts at: 6562 8671 1493 Size: 2109 1697 Fragments arranged by size: 2109 1748 1697 1236 1085 491 226 152 99 32 AseI AT'TA_AT Cuts at: 160 1032 7429 160 Size: 872 6397 1606 Fragments arranged by size: 6397 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8875 AvaI C'yCGr_G Cuts at: 1870 1943 2980 3598 4425 6200 1870 Size: 73 1037 618 827 1775 4545 Fragments arranged by size: 4545 1775 1037 827 618 73 AvrII C'CTAG_G Cuts at: 3967 3967 Size: 8875 BamHI G'GATC_C Cuts at: 2563 3977 6205 6572 2563 Size: 1414 2228 367 4866 Fragments arranged by size: 4866 2228 1414 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2495 2773 2822 3001 3647 Size: 325 217 627 708 278 49 179 646 Cuts at: 3647 5138 5732 6196 7651 618 Size: 1491 594 464 1455 1842 Fragments arranged by size: 1842 1491 1455 708 646 627 594 464 325 278 217 179 49 BanII G_rGCy'C Cuts at: 1391 2979 3336 4995 5555 6081 6194 1391 Size: 1588 357 1659 560 526 113 4072 Fragments arranged by size: 4072 1659 1588 560 526 357 113 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4789 5162 5978 928 Size: 971 2890 373 816 3825 Fragments arranged by size: 3825 2890 971 816 373 BbvCI CC'TCA_GC Cuts at: 4236 4236 Size: 8875 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5830 5864 7066 7100 1903 Size: 34 3893 34 1202 34 3678 Fragments arranged by size: 3893 3678 1202 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4604 6767 7977 4604 Size: 2163 1210 5502 Fragments arranged by size: 5502 2163 1210 BclI T'GATC_A Cuts at: 1548 2919 1548 Size: 1371 7504 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4061 7486 136 Size: 107 122 71 631 605 2389 3425 1525 Fragments arranged by size: 3425 2389 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3801 3892 4645 8870 3801 Size: 91 753 4225 3806 Fragments arranged by size: 4225 3806 753 91 BlpI GC'TnA_GC Cuts at: 6292 6292 Size: 8875 Bme1580I G_kGCm'C Cuts at: 1790 2647 2959 5080 5119 5208 5735 6936 8092 Size: 857 312 2121 39 89 527 1201 1156 Cuts at: 8092 1790 Size: 2573 Fragments arranged by size: 2573 2121 1201 1156 857 527 312 89 39 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8875 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2941 4024 4651 Size: 416 53 83 399 24 1524 1083 627 Cuts at: 4651 4803 6606 7564 442 Size: 152 1803 958 1753 Fragments arranged by size: 1803 1753 1524 1083 958 627 416 399 152 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8875 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3086 3086 Size: 8875 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2288 4268 5069 7535 1239 Size: 1049 1980 801 2466 2579 Fragments arranged by size: 2579 2466 1980 1049 801 Bpu10I CC'TnA_GC Cuts at: 4236 4236 Size: 8875 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3235 3312 3320 3650 7000 7865 8163 3235 Size: 77 8 330 3350 865 298 3947 Fragments arranged by size: 3947 3350 865 330 298 77 8 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4235 4936 5045 7538 882 Size: 617 2736 701 109 2493 2219 Fragments arranged by size: 2736 2493 2219 701 617 109 BsaAI yAC'GTr Cuts at: 493 1204 5566 5863 6666 493 Size: 711 4362 297 803 2702 Fragments arranged by size: 4362 2702 803 711 297 BsaBI GATnn'nnATC Cuts at: 3834 8869 3834 Size: 5035 3840 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6682 7064 7699 275 Size: 53 83 186 936 5149 382 635 1451 Fragments arranged by size: 5149 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3129 3453 4523 7308 7706 7980 8127 8538 3129 Size: 324 1070 2785 398 274 147 411 3466 Fragments arranged by size: 3466 2785 1070 411 398 324 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2261 2291 3366 3396 1178 Size: 30 165 30 858 30 1075 30 6657 Fragments arranged by size: 6657 1075 858 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2096 2378 3647 4253 4728 1186 Size: 910 282 1269 606 475 5333 Fragments arranged by size: 5333 1269 910 606 475 282 BseYI C'CCAG_C Cuts at: 2635 2695 4486 8078 8574 2635 Size: 60 1791 3592 496 2936 Fragments arranged by size: 3592 2936 1791 496 60 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3392 4213 5449 5627 1163 Size: 573 1656 821 1236 178 4411 Fragments arranged by size: 4411 1656 1236 821 573 178 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4567 6325 7086 8114 1540 Size: 275 186 2566 1758 761 1028 2301 Fragments arranged by size: 2566 2301 1758 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2172 2979 3336 5110 5555 6936 7021 8092 2172 Size: 807 357 1774 445 1381 85 1071 2955 Fragments arranged by size: 2955 1774 1381 1071 807 445 357 85 BsmI GAATG_Cn' Cuts at: 2985 4162 5613 6386 6479 2985 Size: 1177 1451 773 93 5381 Fragments arranged by size: 5381 1451 1177 773 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2021 2021 Size: 8875 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2172 2647 2959 2979 3336 4995 5080 Size: 399 382 475 312 20 357 1659 85 Cuts at: 5080 5110 5119 5208 5555 5735 6081 6194 6936 Size: 30 9 89 347 180 346 113 742 Cuts at: 6936 7021 8092 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1659 1071 742 475 399 382 357 347 346 312 180 113 89 85 85 30 20 9 BspEI T'CCGG_A Cuts at: 3129 8538 3129 Size: 5409 3466 BspHI T'CATG_A Cuts at: 2530 4860 5883 6764 8494 2530 Size: 2330 1023 881 1730 2911 Fragments arranged by size: 2911 2330 1730 1023 881 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2345 3861 5162 5706 6230 844 Size: 1501 1516 1301 544 524 3489 Fragments arranged by size: 3489 1516 1501 1301 544 524 BsrBI CCG'CTC Cuts at: 1394 1823 6762 1394 Size: 429 4939 3507 Fragments arranged by size: 4939 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3705 7370 7544 65 Size: 1183 2457 3665 174 1396 Fragments arranged by size: 3665 2457 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4160 4562 7519 7706 1700 Size: 110 2350 402 2957 187 2869 Fragments arranged by size: 2957 2869 2350 402 187 110 BsrGI T'GTAC_A Cuts at: 96 2127 3482 96 Size: 2031 1355 5489 Fragments arranged by size: 5489 2031 1355 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8875 BssSI C'ACGA_G Cuts at: 5303 6935 7947 5303 Size: 1632 1012 6231 Fragments arranged by size: 6231 1632 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2337 4302 5105 5866 794 Size: 1543 1965 803 761 3803 Fragments arranged by size: 3803 1965 1543 803 761 BstBI TT'CG_AA Cuts at: 7679 7679 Size: 8875 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8875 BstXI CCAn_nnnn'nTGG Cuts at: 2480 5126 5602 2480 Size: 2646 476 5753 Fragments arranged by size: 5753 2646 476 BstYI r'GATC_y Cuts at: 1972 2563 2895 3801 3892 3977 4645 4726 5587 Size: 591 332 906 91 85 668 81 861 Cuts at: 5587 6205 6572 6957 6974 8426 8512 8524 8870 Size: 618 367 385 17 1452 86 12 346 Cuts at: 8870 1972 Size: 1977 Fragments arranged by size: 1977 1452 906 861 668 618 591 385 367 346 332 91 86 85 81 17 12 Bsu36I CC'TnA_GG Cuts at: 2079 3240 3818 2079 Size: 1161 578 7136 Fragments arranged by size: 7136 1161 578 BtgI C'CryG_G Cuts at: 513 1065 2004 4084 4341 5826 5836 513 Size: 552 939 2080 257 1485 10 3552 Fragments arranged by size: 3552 2080 1485 939 552 257 10 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3843 487 Size: 683 56 2617 5519 Fragments arranged by size: 5519 2617 683 56 BtsI GCAGTG_nn' Cuts at: 2957 4230 4303 5106 6391 7185 7205 2957 Size: 1273 73 803 1285 794 20 4627 Fragments arranged by size: 4627 1285 1273 803 794 73 20 ClaI AT'CG_AT Cuts at: 1554 3369 1554 Size: 1815 7060 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8840 DraI TTT'AAA Cuts at: 2686 4768 6179 2686 Size: 2082 1411 5382 Fragments arranged by size: 5382 2082 1411 DraIII CAC_nnn'GTG Cuts at: 3786 5723 5725 3786 Size: 1937 2 6936 Fragments arranged by size: 6936 1937 2 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4852 7882 809 Size: 507 3536 3030 1802 Fragments arranged by size: 3536 3030 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2037 3162 3435 Size: 54 1425 50 275 18 207 1125 273 Cuts at: 3435 4120 4560 4564 5712 5928 6322 7211 8840 Size: 685 440 4 1148 216 394 889 1629 Cuts at: 8840 8 Size: 43 Fragments arranged by size: 1629 1425 1148 1125 889 685 440 394 275 273 216 207 54 50 43 18 4 EagI C'GGCC_G Cuts at: 1537 1812 4564 6322 1537 Size: 275 2752 1758 4090 Fragments arranged by size: 4090 2752 1758 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4862 6086 6805 1353 Size: 3509 1224 719 3423 Fragments arranged by size: 3509 3423 1224 719 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7462 7836 7982 387 Size: 1064 138 5873 374 146 1280 Fragments arranged by size: 5873 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2977 3334 5553 2977 Size: 357 2219 6299 Fragments arranged by size: 6299 2219 357 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2281 2288 2399 2570 2721 3059 3445 Size: 393 649 7 111 171 151 338 386 Cuts at: 3445 3790 4154 4268 4283 5069 5174 6938 7535 Size: 345 364 114 15 786 105 1764 597 Cuts at: 7535 8322 8590 1239 Size: 787 268 1524 Fragments arranged by size: 1764 1524 787 786 649 597 393 386 364 345 338 268 171 151 114 111 105 15 7 EcoNI CCTnn'n_nnAGG Cuts at: 4122 5582 4122 Size: 1460 7415 EcoO109I rG'GnC_Cy Cuts at: 3559 4452 6109 6129 3559 Size: 893 1657 20 6305 Fragments arranged by size: 6305 1657 893 20 EcoRI G'AATT_C Cuts at: 6152 6184 6562 6152 Size: 32 378 8465 Fragments arranged by size: 8465 378 32 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3605 3820 5384 3605 Size: 215 1564 7096 Fragments arranged by size: 7096 1564 215 FseI GG_CCGG'CC Cuts at: 4566 4566 Size: 8875 FspI TGC'GCA Cuts at: 7381 7381 Size: 8875 HaeII r_GCGC'y Cuts at: 1678 2487 8022 1678 Size: 809 5535 2531 Fragments arranged by size: 5535 2531 809 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2801 2833 3012 3044 3351 Size: 32 135 32 1229 32 179 32 307 Cuts at: 3351 3383 4028 4060 4637 4669 5445 5477 5622 Size: 32 645 32 577 32 776 32 145 Cuts at: 5622 5654 7516 7548 7590 7622 1373 Size: 32 1862 32 42 32 2626 Fragments arranged by size: 2626 1862 1229 776 645 577 307 179 145 135 42 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4582 6219 6465 669 Size: 988 281 2644 1637 246 3079 Fragments arranged by size: 3079 2644 1637 988 281 246 HindIII A'AGCT_T Cuts at: 748 5675 748 Size: 4927 3948 HpaI GTT'AAC Cuts at: 6465 6465 Size: 8875 KpnI G_GTAC'C Cuts at: 2499 6200 2499 Size: 3701 5174 MfeI C'AATT_G Cuts at: 4095 6474 4095 Size: 2379 6496 MluI A'CGCG_T Cuts at: 8751 8751 Size: 8875 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3055 5901 7989 8173 1305 Size: 1750 2846 2088 184 2007 Fragments arranged by size: 2846 2088 2007 1750 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2039 3164 3437 4122 5714 Size: 54 1425 343 207 1125 273 685 1592 Cuts at: 5714 5930 10 Size: 216 2955 Fragments arranged by size: 2955 1592 1425 1125 685 343 273 216 207 54 MslI CAynn'nnrTG Cuts at: 518 1166 2478 5144 5828 6047 6833 7192 7351 Size: 648 1312 2666 684 219 786 359 159 Cuts at: 7351 518 Size: 2042 Fragments arranged by size: 2666 2042 1312 786 684 648 359 219 159 MspA1I CmG'CkG Cuts at: 1606 2699 2947 3616 4490 4601 5011 6291 6968 Size: 1093 248 669 874 111 410 1280 677 Cuts at: 6968 8116 8361 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1280 1148 1093 874 677 669 410 248 245 111 NaeI GCC'GGC Cuts at: 4162 4564 4162 Size: 402 8473 NcoI C'CATG_G Cuts at: 513 1065 2004 4341 5826 513 Size: 552 939 2337 1485 3562 Fragments arranged by size: 3562 2337 1485 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8875 NgoMIV G'CCGG_C Cuts at: 4160 4562 4160 Size: 402 8473 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8875 NotI GC'GGCC_GC Cuts at: 6322 6322 Size: 8875 NspI r_CATG'y Cuts at: 1132 1229 2150 4618 6233 7778 1132 Size: 97 921 2468 1615 1545 2229 Fragments arranged by size: 2468 2229 1615 1545 921 97 PasI CC'CwG_GG Cuts at: 1348 5197 1348 Size: 3849 5026 PciI A'CATG_T Cuts at: 7774 7774 Size: 8875 PflMI CCAn_nnn'nTGG Cuts at: 1108 2182 3012 4558 1108 Size: 1074 830 1546 5425 Fragments arranged by size: 5425 1546 1074 830 PfoI T'CCnGG_A Cuts at: 2022 4308 2022 Size: 2286 6589 PmeI GTTT'AAAC Cuts at: 6179 6179 Size: 8875 PmlI CAC'GTG Cuts at: 5863 5863 Size: 8875 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6944 6976 8457 8489 6944 Size: 32 1481 32 7330 Fragments arranged by size: 7330 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4452 6109 4452 Size: 1657 7218 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8875 PsiI TTA'TAA Cuts at: 6445 6445 Size: 8875 PstI C_TGCA'G Cuts at: 830 2946 2952 4036 4215 4735 4905 5101 5574 Size: 2116 6 1084 179 520 170 196 473 Cuts at: 5574 6227 830 Size: 653 3478 Fragments arranged by size: 3478 2116 1084 653 520 473 196 179 170 6 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8875 PvuII CAG'CTG Cuts at: 2699 2947 4490 4601 5011 2699 Size: 248 1543 111 410 6563 Fragments arranged by size: 6563 1543 410 248 111 SacI G_AGCT'C Cuts at: 2979 3336 5555 2979 Size: 357 2219 6299 Fragments arranged by size: 6299 2219 357 SalI G'TCGA_C Cuts at: 1936 4580 6217 1936 Size: 2644 1637 4594 Fragments arranged by size: 4594 2644 1637 SanDI GG'GwC_CC Cuts at: 6109 6109 Size: 8875 SapI GCTCTTCn'nnn_ Cuts at: 6086 6086 Size: 8875 SbfI CC_TGCA'GG Cuts at: 4905 6227 4905 Size: 1322 7553 ScaI AGT'ACT Cuts at: 3875 7123 8835 3875 Size: 3248 1712 3915 Fragments arranged by size: 3915 3248 1712 SfcI C'TryA_G Cuts at: 826 1045 2942 2948 4032 4211 4731 4901 5056 Size: 219 1897 6 1084 179 520 170 155 Cuts at: 5056 5097 5570 6223 7358 8039 8230 826 Size: 41 473 653 1135 681 191 1471 Fragments arranged by size: 1897 1471 1135 1084 681 653 520 473 219 191 179 170 155 41 6 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8875 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8875 SmaI CCC'GGG Cuts at: 1872 6202 1872 Size: 4330 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3214 3327 3335 3665 4425 6979 Size: 197 926 1271 113 8 330 760 2554 Cuts at: 6979 7880 8142 820 Size: 901 262 1553 Fragments arranged by size: 2554 1553 1271 926 901 760 330 262 197 113 8 SnaBI TAC'GTA Cuts at: 493 6666 493 Size: 6173 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8875 SphI G_CATG'C Cuts at: 1132 6233 1132 Size: 5101 3774 SspI AAT'ATT Cuts at: 5 52 3991 6799 5 Size: 47 3939 2808 2081 Fragments arranged by size: 3939 2808 2081 47 StuI AGG'CCT Cuts at: 1517 3823 1517 Size: 2306 6569 StyI C'CwwG_G Cuts at: 513 1065 2004 3690 3861 3967 4123 4341 5235 Size: 552 939 1686 171 106 156 218 894 Cuts at: 5235 5595 5826 6314 513 Size: 360 231 488 3074 Fragments arranged by size: 3074 1686 939 894 552 488 360 231 218 171 156 106 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3019 3264 5045 5093 5156 6902 6919 Size: 1255 1439 245 1781 48 63 1746 17 Cuts at: 6919 7072 7257 7702 325 Size: 153 185 445 1498 Fragments arranged by size: 1781 1746 1498 1439 1255 445 245 185 153 63 48 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2127 3482 3873 Size: 274 80 33 51 1443 150 1355 391 Cuts at: 3873 4182 5067 5559 5670 5820 7121 8833 96 Size: 309 885 492 111 150 1301 1712 138 Fragments arranged by size: 1712 1443 1355 1301 885 492 391 309 274 150 150 138 111 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2261 2293 3069 3101 3366 3398 6008 6040 8835 Size: 32 776 32 265 32 2610 32 2795 Cuts at: 8835 8867 2261 Size: 32 2269 Fragments arranged by size: 2795 2610 2269 776 265 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8875 XbaI T'CTAG_A Cuts at: 6211 6211 Size: 8875 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2060 2554 4501 4706 2060 Size: 494 1947 205 6229 Fragments arranged by size: 6229 1947 494 205 XhoI C'TCGA_G Cuts at: 1943 4425 1943 Size: 2482 6393 XmaI C'CCGG_G Cuts at: 1870 6200 1870 Size: 4330 4545 XmnI GAAnn'nnTTC Cuts at: 2706 5043 5159 5364 7004 2706 Size: 2337 116 205 1640 4577 Fragments arranged by size: 4577 2337 1640 205 116 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6683 276 Size: 53 83 186 936 5149 2468 Fragments arranged by size: 5149 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI BaeI BbeI BfrBI BsiWI BstZ17I EcoRV FspAI KasI NarI NruI NsiI PacI PspOMI PspXI PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI