(Circular) (Six-base) MAPSORT of: pFN21AB3954.seq Check: 3478 from: 1 to: 9103 pFN21AB3954 9103 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2945 3287 2945 Size: 342 8761 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6913 278 Size: 53 83 186 936 5377 2468 Fragments arranged by size: 5377 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2707 3688 6446 1937 Size: 770 981 2758 4594 Fragments arranged by size: 4594 2758 981 770 Acc65I G'GTAC_C Cuts at: 6424 6424 Size: 9103 AclI AA'CG_TT Cuts at: 1563 7230 7603 1563 Size: 5667 373 3063 Fragments arranged by size: 5667 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2622 6063 6194 6231 7166 8550 8818 1632 Size: 990 3441 131 37 935 1384 268 1917 Fragments arranged by size: 3441 1917 1384 990 935 268 131 37 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8906 AflIII A'CryG_T Cuts at: 1203 5977 8002 8979 1203 Size: 4774 2025 977 1327 Fragments arranged by size: 4774 2025 1327 977 AgeI A'CCGG_T Cuts at: 7934 7934 Size: 9103 AhdI GACnn_n'nnGTC Cuts at: 7832 7832 Size: 9103 AleI CACnn'nnGTG Cuts at: 1166 3135 1166 Size: 1969 7134 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 9103 AlwNI CAG_nnn'CTG Cuts at: 3446 3545 4256 4427 4724 5372 5408 6846 8418 Size: 99 711 171 297 648 36 1438 1572 Cuts at: 8418 3446 Size: 4131 Fragments arranged by size: 4131 1572 1438 711 648 297 171 99 36 ApaI G_GGCC'C Cuts at: 3555 4078 4716 4823 3555 Size: 523 638 107 7835 Fragments arranged by size: 7835 638 523 107 ApaLI G'TGCA_C Cuts at: 4002 7160 8316 4002 Size: 3158 1156 4789 Fragments arranged by size: 4789 3158 1156 ApoI r'AATT_y Cuts at: 1493 2238 5801 6200 6224 6368 6412 6638 6790 Size: 745 3563 399 24 144 44 226 152 Cuts at: 6790 8899 1493 Size: 2109 1697 Fragments arranged by size: 3563 2109 1697 745 399 226 152 144 44 24 AseI AT'TA_AT Cuts at: 160 1032 3168 7657 160 Size: 872 2136 4489 1606 Fragments arranged by size: 4489 2136 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9103 AvaI C'yCGr_G Cuts at: 1870 1943 2961 2979 3240 3717 5247 5499 6428 Size: 73 1018 18 261 477 1530 252 929 Cuts at: 6428 1870 Size: 4545 Fragments arranged by size: 4545 1530 1018 929 477 261 252 73 18 AvrII C'CTAG_G Cuts at: 4904 4904 Size: 9103 BamHI G'GATC_C Cuts at: 2177 3038 6433 6800 2177 Size: 861 3395 367 4480 Fragments arranged by size: 4480 3395 861 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3092 3317 3461 4037 4352 Size: 325 217 627 1305 225 144 576 315 Cuts at: 4352 4811 5126 5351 5576 6424 7879 618 Size: 459 315 225 225 848 1455 1842 Fragments arranged by size: 1842 1455 1305 848 627 576 459 325 315 315 225 225 225 217 144 BanII G_rGCy'C Cuts at: 1391 2890 2917 3223 3241 3333 3555 4015 4078 Size: 1499 27 306 18 92 222 460 63 Cuts at: 4078 4492 4716 4823 4834 5534 6422 1391 Size: 414 224 107 11 700 888 4072 Fragments arranged by size: 4072 1499 888 700 460 414 306 224 222 107 92 63 27 18 11 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2861 4634 4994 5057 928 Size: 971 962 1773 360 63 4974 Fragments arranged by size: 4974 1773 971 962 360 63 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 7294 7328 1903 Size: 34 5357 34 3678 Fragments arranged by size: 5357 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2591 2763 6995 8205 2591 Size: 172 4232 1210 3489 Fragments arranged by size: 4232 3489 1210 172 BclI T'GATC_A Cuts at: 1548 6361 1548 Size: 4813 4290 BfrBI ATG'CAT Cuts at: 5910 5910 Size: 9103 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2541 3874 4323 Size: 107 122 71 631 605 869 1333 449 Cuts at: 4323 5214 7714 136 Size: 891 2500 1525 Fragments arranged by size: 2500 1525 1333 891 869 631 605 449 122 107 71 BglII A'GATC_T Cuts at: 9098 9098 Size: 9103 BlpI GC'TnA_GC Cuts at: 6520 6520 Size: 9103 Bme1580I G_kGCm'C Cuts at: 1790 3097 3466 3555 4006 4042 4078 4357 4716 Size: 1307 369 89 451 36 36 279 359 Cuts at: 4716 4816 4823 5131 5356 5581 7164 8320 1790 Size: 100 7 308 225 225 1583 1156 2573 Fragments arranged by size: 2573 1583 1307 1156 451 369 359 308 279 225 225 100 89 36 36 7 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 9103 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2458 3882 5430 Size: 416 53 83 399 24 1041 1424 1548 Cuts at: 5430 5845 5907 6276 6834 7792 442 Size: 415 62 369 558 958 1753 Fragments arranged by size: 1753 1548 1424 1041 958 558 416 415 399 369 83 62 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9103 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2511 2577 2631 2730 2742 3234 3237 3435 Size: 1272 66 54 99 12 492 3 198 Cuts at: 3435 3522 3543 3588 3615 3822 3936 3999 4029 Size: 87 21 45 27 207 114 63 30 Cuts at: 4029 4209 4353 4404 4542 4560 4683 4776 4818 Size: 180 144 51 138 18 123 93 42 Cuts at: 4818 4884 5349 6070 6263 7763 1239 Size: 66 465 721 193 1500 2579 Fragments arranged by size: 2579 1500 1272 721 492 465 207 198 193 180 144 138 123 114 99 93 87 66 66 63 54 51 45 42 30 27 21 18 12 3 Bpu10I CC'TnA_GC Cuts at: 3051 3051 Size: 9103 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2301 3126 5250 5702 5804 7228 8093 8391 2301 Size: 825 2124 452 102 1424 865 298 3013 Fragments arranged by size: 3013 2124 1424 865 825 452 298 102 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3081 4970 5787 7766 882 Size: 617 1582 1889 817 1979 2219 Fragments arranged by size: 2219 1979 1889 1582 817 617 BsaAI yAC'GTr Cuts at: 493 1204 3341 5276 6894 493 Size: 711 2137 1935 1618 2702 Fragments arranged by size: 2702 2137 1935 1618 711 BsaBI GATnn'nnATC Cuts at: 9097 9097 Size: 9103 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3588 6910 7292 7927 Size: 53 83 186 936 2055 3322 382 635 Cuts at: 7927 275 Size: 1451 Fragments arranged by size: 3322 2055 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 7536 7934 8208 8355 8766 7536 Size: 398 274 147 411 7873 Fragments arranged by size: 7873 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2281 2311 2350 2380 2484 Size: 30 165 30 878 30 39 30 104 Cuts at: 2484 2514 2827 2857 4402 4432 4726 4756 1178 Size: 30 313 30 1545 30 294 30 5525 Fragments arranged by size: 5525 1545 878 313 294 165 104 39 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3123 3573 4212 5211 1186 Size: 1937 450 639 999 5078 Fragments arranged by size: 5078 1937 999 639 450 BseYI C'CCAG_C Cuts at: 2765 4318 4688 4853 5621 8306 8802 2765 Size: 1553 370 165 768 2685 496 3066 Fragments arranged by size: 3066 2685 1553 768 496 370 165 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2280 3315 6080 1163 Size: 573 544 1035 2765 4186 Fragments arranged by size: 4186 2765 1035 573 544 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6553 7314 8342 1540 Size: 275 186 4552 761 1028 2301 Fragments arranged by size: 4552 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2845 2890 2962 2980 3142 3223 3277 3538 3772 Size: 45 72 18 162 81 54 261 234 Cuts at: 3772 4006 4015 4447 4636 4744 4834 4870 5230 Size: 234 9 432 189 108 90 36 360 Cuts at: 5230 5257 7164 7249 8320 2845 Size: 27 1907 85 1071 3628 Fragments arranged by size: 3628 1907 1071 432 360 261 234 234 189 162 108 90 85 81 72 54 45 36 27 18 9 BsmI GAATG_Cn' Cuts at: 3210 4188 6068 6614 6707 3210 Size: 978 1880 546 93 5606 Fragments arranged by size: 5606 1880 978 546 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2845 2890 2917 2962 2980 3097 3142 Size: 399 1055 45 27 45 18 117 45 Cuts at: 3142 3223 3241 3277 3333 3466 3538 3555 3772 Size: 81 18 36 56 133 72 17 217 Cuts at: 3772 4006 4015 4042 4078 4357 4447 4492 4636 Size: 234 9 27 36 279 90 45 144 Cuts at: 4636 4716 4744 4816 4823 4834 4870 5131 5230 Size: 80 28 72 7 11 36 261 99 Cuts at: 5230 5257 5356 5534 5581 6422 7164 7249 8320 Size: 27 99 178 47 841 742 85 1071 Cuts at: 8320 1391 Size: 2174 Fragments arranged by size: 2174 1071 1055 841 742 399 279 261 234 217 178 144 133 117 99 99 90 85 81 80 72 72 56 47 45 45 45 45 36 36 36 28 27 27 27 18 18 17 11 9 7 BspEI T'CCGG_A Cuts at: 8766 8766 Size: 9103 BspHI T'CATG_A Cuts at: 6992 8722 6992 Size: 1730 7373 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2945 3076 3287 3490 4435 4561 5416 6458 Size: 2101 131 211 203 945 126 855 1042 Cuts at: 6458 844 Size: 3489 Fragments arranged by size: 3489 2101 1042 945 855 211 203 131 126 BsrBI CCG'CTC Cuts at: 1394 1823 6990 1394 Size: 429 5167 3507 Fragments arranged by size: 5167 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5616 6129 7598 7772 65 Size: 1183 4368 513 1469 174 1396 Fragments arranged by size: 4368 1469 1396 1183 513 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3260 3998 7747 7934 1700 Size: 110 1450 738 3749 187 2869 Fragments arranged by size: 3749 2869 1450 738 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 9103 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9103 BssSI C'ACGA_G Cuts at: 4383 4635 7163 8175 4383 Size: 252 2528 1012 5311 Fragments arranged by size: 5311 2528 1012 252 BstAPI GCAn_nnn'nTGC Cuts at: 794 4183 794 Size: 3389 5714 BstBI TT'CG_AA Cuts at: 7907 7907 Size: 9103 BstEII G'GTnAC_C Cuts at: 1184 2366 4667 1184 Size: 1182 2301 5620 Fragments arranged by size: 5620 2301 1182 BstYI r'GATC_y Cuts at: 1972 2177 2332 2588 3038 5081 5522 6433 6800 Size: 205 155 256 450 2043 441 911 367 Cuts at: 6800 7185 7202 8654 8740 8752 9098 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 2043 1977 1452 911 450 441 385 367 346 256 205 155 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3123 3816 3852 4923 3123 Size: 693 36 1071 7303 Fragments arranged by size: 7303 1071 693 36 BtgI C'CryG_G Cuts at: 513 1065 2898 5936 5997 513 Size: 552 1833 3038 61 3619 Fragments arranged by size: 3619 3038 1833 552 61 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2205 487 Size: 683 56 979 7385 Fragments arranged by size: 7385 979 683 56 BtsI GCAGTG_nn' Cuts at: 2992 6115 6619 7413 7433 2992 Size: 3123 504 794 20 4662 Fragments arranged by size: 4662 3123 794 504 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9103 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9068 DraI TTT'AAA Cuts at: 6407 6407 Size: 9103 DraIII CAC_nnn'GTG Cuts at: 3344 4154 5279 3344 Size: 810 1125 7168 Fragments arranged by size: 7168 1125 810 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2901 3909 4557 4683 4998 5475 8110 Size: 507 1585 1008 648 126 315 477 2635 Cuts at: 8110 809 Size: 1802 Fragments arranged by size: 2635 1802 1585 1008 648 507 477 315 126 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2933 4324 4783 Size: 54 1425 50 275 18 1103 1391 459 Cuts at: 4783 6550 7439 9068 8 Size: 1767 889 1629 43 Fragments arranged by size: 1767 1629 1425 1391 1103 889 459 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6550 1537 Size: 275 4738 4090 Fragments arranged by size: 4738 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 5032 7033 1353 Size: 3679 2001 3423 Fragments arranged by size: 3679 3423 2001 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7690 8064 8210 387 Size: 1064 138 6101 374 146 1280 Fragments arranged by size: 6101 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2888 3221 4013 4832 2888 Size: 333 792 819 7159 Fragments arranged by size: 7159 819 792 333 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2511 2577 2622 2631 2730 2742 3234 Size: 393 879 66 45 9 99 12 492 Cuts at: 3234 3237 3435 3522 3543 3588 3615 3822 3936 Size: 3 198 87 21 45 27 207 114 Cuts at: 3936 3999 4029 4209 4353 4404 4542 4560 4683 Size: 63 30 180 144 51 138 18 123 Cuts at: 4683 4776 4818 4884 5349 6063 6070 6194 6231 Size: 93 42 66 465 714 7 124 37 Cuts at: 6231 6263 7166 7763 8550 8818 1239 Size: 32 903 597 787 268 1524 Fragments arranged by size: 1524 903 879 787 714 597 492 465 393 268 207 198 180 144 138 124 123 114 99 93 87 66 66 63 51 45 45 42 37 32 30 27 21 18 12 9 7 3 EcoNI CCTnn'n_nnAGG Cuts at: 3939 5850 3939 Size: 1911 7192 EcoO109I rG'GnC_Cy Cuts at: 2312 2321 2339 2394 2543 2606 2642 2651 3002 Size: 9 18 55 149 63 36 9 351 Cuts at: 3002 3146 3200 3245 3479 3515 3542 3551 3659 Size: 144 54 45 234 36 27 9 108 Cuts at: 3659 3695 3806 3821 3857 3866 3902 4074 4075 Size: 36 111 15 36 9 36 172 1 Cuts at: 4075 4289 4424 4514 4532 4676 4685 4713 4721 Size: 214 135 90 18 144 9 28 8 Cuts at: 4721 4819 4901 5369 5405 5414 5423 5477 5495 Size: 98 82 468 36 9 9 54 18 Cuts at: 5495 5558 6169 2312 Size: 63 611 5246 Fragments arranged by size: 5246 611 468 351 234 214 172 149 144 144 135 111 108 98 90 82 63 63 55 54 54 45 36 36 36 36 36 36 28 27 18 18 18 15 9 9 9 9 9 9 9 8 1 EcoRI G'AATT_C Cuts at: 6200 6412 6790 6200 Size: 212 378 8513 Fragments arranged by size: 8513 378 212 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 6068 6068 Size: 9103 FspI TGC'GCA Cuts at: 7609 7609 Size: 9103 HaeII r_GCGC'y Cuts at: 1678 5139 8250 1678 Size: 3461 3111 2531 Fragments arranged by size: 3461 3111 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2162 2169 2194 2201 2471 Size: 32 135 32 590 7 25 7 270 Cuts at: 2471 2503 2684 2716 3573 3605 4787 4819 7744 Size: 32 181 32 857 32 1182 32 2925 Cuts at: 7744 7776 7818 7850 1373 Size: 32 42 32 2626 Fragments arranged by size: 2925 2626 1182 857 590 270 181 135 42 32 32 32 32 32 32 32 32 25 7 7 HincII GTy'rAC Cuts at: 669 1657 1938 2996 3689 5842 6447 6693 669 Size: 988 281 1058 693 2153 605 246 3079 Fragments arranged by size: 3079 2153 1058 988 693 605 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 9103 HpaI GTT'AAC Cuts at: 6693 6693 Size: 9103 KpnI G_GTAC'C Cuts at: 6428 6428 Size: 9103 MfeI C'AATT_G Cuts at: 6702 6702 Size: 9103 MluI A'CGCG_T Cuts at: 8979 8979 Size: 9103 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2303 3043 5410 5584 8217 8401 1305 Size: 998 740 2367 174 2633 184 2007 Fragments arranged by size: 2633 2367 2007 998 740 184 174 MscI TGG'CCA Cuts at: 10 64 1489 1832 4326 4785 10 Size: 54 1425 343 2494 459 4328 Fragments arranged by size: 4328 2494 1425 459 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3135 7061 7420 7579 518 Size: 648 1969 3926 359 159 2042 Fragments arranged by size: 3926 2042 1969 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2531 2765 3206 4322 4692 5024 5177 5612 Size: 925 234 441 1116 370 332 153 435 Cuts at: 5612 6064 6519 7196 8344 8589 1606 Size: 452 455 677 1148 245 2120 Fragments arranged by size: 2120 1148 1116 925 677 455 452 441 435 370 332 245 234 153 NcoI C'CATG_G Cuts at: 513 1065 2898 5997 513 Size: 552 1833 3099 3619 Fragments arranged by size: 3619 3099 1833 552 NdeI CA'TA_TG Cuts at: 387 2476 387 Size: 2089 7014 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9103 NotI GC'GGCC_GC Cuts at: 6550 6550 Size: 9103 NsiI A_TGCA'T Cuts at: 5912 5912 Size: 9103 NspI r_CATG'y Cuts at: 1132 1229 3619 4897 5910 6461 8006 1132 Size: 97 2390 1278 1013 551 1545 2229 Fragments arranged by size: 2390 2229 1545 1278 1013 551 97 PasI CC'CwG_GG Cuts at: 1348 3467 3863 4268 4816 4996 5546 1348 Size: 2119 396 405 548 180 550 4905 Fragments arranged by size: 4905 2119 550 548 405 396 180 PciI A'CATG_T Cuts at: 8002 8002 Size: 9103 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 9103 PfoI T'CCnGG_A Cuts at: 2380 2521 2593 2647 2845 2890 3016 3214 3223 Size: 141 72 54 198 45 126 198 9 Cuts at: 3223 3250 3277 3376 3439 3601 3628 3664 3826 Size: 27 27 99 63 162 27 36 162 Cuts at: 3826 3952 4015 4060 4420 4447 4519 5401 5563 Size: 126 63 45 360 27 72 882 162 Cuts at: 5563 2380 Size: 5920 Fragments arranged by size: 5920 882 360 198 198 162 162 162 141 126 126 99 72 72 63 63 54 45 45 36 27 27 27 27 9 PmeI GTTT'AAAC Cuts at: 6407 6407 Size: 9103 PmlI CAC'GTG Cuts at: 3341 5276 3341 Size: 1935 7168 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7172 7204 8685 8717 7172 Size: 32 1481 32 7558 Fragments arranged by size: 7558 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2312 2394 2606 2651 3146 3200 3245 3479 3515 Size: 82 212 45 495 54 45 234 36 Cuts at: 3515 3695 3806 3821 3857 3902 4289 4424 4532 Size: 180 111 15 36 45 387 135 108 Cuts at: 4532 4676 4685 4721 4901 5405 5423 5477 2312 Size: 144 9 36 180 504 18 54 5938 Fragments arranged by size: 5938 504 495 387 234 212 180 180 144 135 111 108 82 54 54 45 45 45 36 36 36 18 15 9 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 9103 PsiI TTA'TAA Cuts at: 6673 6673 Size: 9103 PspOMI G'GGCC_C Cuts at: 3551 4074 4712 4819 3551 Size: 523 638 107 7835 Fragments arranged by size: 7835 638 523 107 PspXI vC'TCGA_Gb Cuts at: 2979 5499 2979 Size: 2520 6583 PstI C_TGCA'G Cuts at: 830 2956 3073 3451 3550 5413 5792 6455 830 Size: 2126 117 378 99 1863 379 663 3478 Fragments arranged by size: 3478 2126 1863 663 379 378 117 99 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9103 PvuII CAG'CTG Cuts at: 2531 2765 4322 5024 5177 6064 2531 Size: 234 1557 702 153 887 5570 Fragments arranged by size: 5570 1557 887 702 234 153 SacI G_AGCT'C Cuts at: 2890 3223 4015 4834 2890 Size: 333 792 819 7159 Fragments arranged by size: 7159 819 792 333 SalI G'TCGA_C Cuts at: 1936 3687 6445 1936 Size: 1751 2758 4594 Fragments arranged by size: 4594 2758 1751 SanDI GG'GwC_CC Cuts at: 2394 3200 3857 2394 Size: 806 657 7640 Fragments arranged by size: 7640 806 657 SbfI CC_TGCA'GG Cuts at: 3073 3550 5413 6455 3073 Size: 477 1863 1042 5721 Fragments arranged by size: 5721 1863 1042 477 ScaI AGT'ACT Cuts at: 7351 9063 7351 Size: 1712 7391 SexAI A'CCwGG_T Cuts at: 3421 3691 3979 4150 4465 4609 5158 3421 Size: 270 288 171 315 144 549 7366 Fragments arranged by size: 7366 549 315 288 270 171 144 SfcI C'TryA_G Cuts at: 826 1045 2664 2952 3069 3447 3546 5409 5788 Size: 219 1619 288 117 378 99 1863 379 Cuts at: 5788 6451 7586 8267 8458 826 Size: 663 1135 681 191 1471 Fragments arranged by size: 1863 1619 1471 1135 681 663 379 378 288 219 191 117 99 SfiI GGCCn_nnn'nGGCC Cuts at: 2541 3874 4323 5214 2541 Size: 1333 449 891 6430 Fragments arranged by size: 6430 1333 891 449 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9103 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9103 SmaI CCC'GGG Cuts at: 1872 3242 6430 1872 Size: 1370 3188 4545 Fragments arranged by size: 4545 3188 1370 SmlI C'TyrA_G Cuts at: 820 1017 1943 2316 2979 3141 5265 5499 5717 Size: 197 926 373 663 162 2124 234 218 Cuts at: 5717 5819 7207 8108 8370 820 Size: 102 1388 901 262 1553 Fragments arranged by size: 2124 1553 1388 926 901 663 373 262 234 218 197 162 102 SnaBI TAC'GTA Cuts at: 493 6894 493 Size: 6401 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 9103 SphI G_CATG'C Cuts at: 1132 3619 4897 6461 1132 Size: 2487 1278 1564 3774 Fragments arranged by size: 3774 2487 1564 1278 SspI AAT'ATT Cuts at: 5 52 7027 5 Size: 47 6975 2081 Fragments arranged by size: 6975 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 9103 StyI C'CwwG_G Cuts at: 513 1065 2325 2602 2898 4283 4573 4904 5851 Size: 552 1260 277 296 1385 290 331 947 Cuts at: 5851 5997 6542 513 Size: 146 545 3074 Fragments arranged by size: 3074 1385 1260 947 552 545 331 296 290 277 146 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5159 5559 7130 7147 7300 7485 7930 Size: 1255 3579 400 1571 17 153 185 445 Cuts at: 7930 325 Size: 1498 Fragments arranged by size: 3579 1571 1498 1255 445 400 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 7349 9061 96 Size: 274 80 33 51 1443 5372 1712 138 Fragments arranged by size: 5372 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2350 2382 4726 4758 5617 5649 9063 9095 2350 Size: 32 2344 32 859 32 3414 32 2358 Fragments arranged by size: 3414 2358 2344 859 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3693 1316 Size: 2377 6726 XbaI T'CTAG_A Cuts at: 6439 6439 Size: 9103 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2065 2609 5003 5943 2065 Size: 544 2394 940 5225 Fragments arranged by size: 5225 2394 940 544 XhoI C'TCGA_G Cuts at: 1943 2979 5499 1943 Size: 1036 2520 5547 Fragments arranged by size: 5547 2520 1036 XmaI C'CCGG_G Cuts at: 1870 3240 6428 1870 Size: 1370 3188 4545 Fragments arranged by size: 4545 3188 1370 XmnI GAAnn'nnTTC Cuts at: 7232 7232 Size: 9103 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6911 276 Size: 53 83 186 936 5377 2468 Fragments arranged by size: 5377 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BaeI BbeI BbvCI BplI BsiWI BsmBI BstXI BstZ17I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PsrI RsrII SacII SapI SfoI SrfI SwaI