(Circular) (Six-base) MAPSORT of: pFN21AB3816.seq Check: 9397 from: 1 to: 10741 pFN21AB3816 10741 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3190 4016 3190 Size: 826 9915 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4332 8551 278 Size: 53 83 186 936 2796 4219 2468 Fragments arranged by size: 4219 2796 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3447 5124 7341 8084 1937 Size: 1510 1677 2217 743 4594 Fragments arranged by size: 4594 2217 1677 1510 743 Acc65I G'GTAC_C Cuts at: 2178 3045 8062 2178 Size: 867 5017 4857 Fragments arranged by size: 5017 4857 867 AclI AA'CG_TT Cuts at: 1563 6304 8868 9241 1563 Size: 4741 2564 373 3063 Fragments arranged by size: 4741 3063 2564 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2260 2310 2801 5784 5860 6924 7835 8804 Size: 628 50 491 2983 76 1064 911 969 Cuts at: 8804 10188 10456 1632 Size: 1384 268 1917 Fragments arranged by size: 2983 1917 1384 1064 969 911 628 491 268 76 50 AfeI AGC'GCT Cuts at: 2748 2748 Size: 10741 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 10544 AflIII A'CryG_T Cuts at: 1203 3723 3732 6475 7527 9640 10617 1203 Size: 2520 9 2743 1052 2113 977 1327 Fragments arranged by size: 2743 2520 2113 1327 1052 977 9 AgeI A'CCGG_T Cuts at: 9572 9572 Size: 10741 AhdI GACnn_n'nnGTC Cuts at: 3178 5985 9470 3178 Size: 2807 3485 4449 Fragments arranged by size: 4449 3485 2807 AleI CACnn'nnGTG Cuts at: 1166 3579 6476 1166 Size: 2413 2897 5431 Fragments arranged by size: 5431 2897 2413 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3172 5513 1184 Size: 1988 2341 6412 Fragments arranged by size: 6412 2341 1988 AlwNI CAG_nnn'CTG Cuts at: 2944 3023 4955 5500 5781 6451 6970 8484 10056 Size: 79 1932 545 281 670 519 1514 1572 Cuts at: 10056 2944 Size: 3629 Fragments arranged by size: 3629 1932 1572 1514 670 545 519 281 79 ApaI G_GGCC'C Cuts at: 6380 7155 7224 6380 Size: 775 69 9897 Fragments arranged by size: 9897 775 69 ApaLI G'TGCA_C Cuts at: 8798 9954 8798 Size: 1156 9585 ApoI r'AATT_y Cuts at: 1493 2250 4175 4359 5361 5745 8050 8276 8428 Size: 757 1925 184 1002 384 2305 226 152 Cuts at: 8428 10537 1493 Size: 2109 1697 Fragments arranged by size: 2305 2109 1925 1697 1002 757 384 226 184 152 AseI AT'TA_AT Cuts at: 160 1032 4608 9295 160 Size: 872 3576 4687 1606 Fragments arranged by size: 4687 3576 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 10741 AvaI C'yCGr_G Cuts at: 1870 1943 2213 2750 5255 7989 8066 1870 Size: 73 270 537 2505 2734 77 4545 Fragments arranged by size: 4545 2734 2505 537 270 77 73 BamHI G'GATC_C Cuts at: 2217 8071 8438 2217 Size: 5854 367 4520 Fragments arranged by size: 5854 4520 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2134 2178 2754 3045 7754 Size: 325 217 627 347 44 576 291 4709 Cuts at: 7754 8062 9517 618 Size: 308 1455 1842 Fragments arranged by size: 4709 1842 1455 627 576 347 325 308 291 217 44 BanII G_rGCy'C Cuts at: 1391 2379 3962 4659 4917 4929 6380 6429 6783 Size: 988 1583 697 258 12 1451 49 354 Cuts at: 6783 7000 7155 7224 7839 8060 1391 Size: 217 155 69 615 221 4072 Fragments arranged by size: 4072 1583 1451 988 697 615 354 258 221 217 155 69 49 12 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2064 2422 2828 3835 4287 6066 6171 Size: 971 165 358 406 1007 452 1779 105 Cuts at: 6171 928 Size: 5498 Fragments arranged by size: 5498 1779 1007 971 452 406 358 165 105 BbvCI CC'TCA_GC Cuts at: 4143 4824 5605 7920 4143 Size: 681 781 2315 6964 Fragments arranged by size: 6964 2315 781 681 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2731 2765 8932 8966 1903 Size: 34 794 34 6167 34 3678 Fragments arranged by size: 6167 3678 794 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2004 6230 6818 8633 9843 2004 Size: 4226 588 1815 1210 2902 Fragments arranged by size: 4226 2902 1815 1210 588 BclI T'GATC_A Cuts at: 1548 3378 4260 5703 1548 Size: 1830 882 1443 6586 Fragments arranged by size: 6586 1830 1443 882 BfrBI ATG'CAT Cuts at: 2090 2090 Size: 10741 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 9352 136 Size: 107 122 71 631 605 7680 1525 Fragments arranged by size: 7680 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2199 5541 10736 2199 Size: 3342 5195 2204 Fragments arranged by size: 5195 3342 2204 BlpI GC'TnA_GC Cuts at: 6999 7089 8158 6999 Size: 90 1069 9582 Fragments arranged by size: 9582 1069 90 Bme1580I G_kGCm'C Cuts at: 1790 2139 2757 5268 6380 7155 7224 8802 9958 Size: 349 618 2511 1112 775 69 1578 1156 Cuts at: 9958 1790 Size: 2573 Fragments arranged by size: 2573 2511 1578 1156 1112 775 618 349 69 BmgBI CAC'GTC Cuts at: 1318 2227 3384 6177 7530 1318 Size: 909 1157 2793 1353 4529 Fragments arranged by size: 4529 2793 1353 1157 909 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4195 5100 5262 Size: 416 53 83 399 24 2778 905 162 Cuts at: 5262 5398 5979 6106 6424 7158 8472 9430 442 Size: 136 581 127 318 734 1314 958 1753 Fragments arranged by size: 2778 1753 1314 958 905 734 581 416 399 318 162 136 127 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 10741 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6603 6603 Size: 10741 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2053 3403 5003 5351 5474 6228 7327 7567 Size: 814 1350 1600 348 123 754 1099 240 Cuts at: 7567 7853 9401 1239 Size: 286 1548 2579 Fragments arranged by size: 2579 1600 1548 1350 1099 814 754 348 286 240 123 Bpu10I CC'TnA_GC Cuts at: 2760 2910 4143 4712 4824 5605 7769 7774 7920 Size: 150 1233 569 112 781 2164 5 146 Cuts at: 7920 2760 Size: 5581 Fragments arranged by size: 5581 2164 1233 781 569 150 146 112 5 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3171 3332 3985 5627 6767 8866 9731 10029 3171 Size: 161 653 1642 1140 2099 865 298 3883 Fragments arranged by size: 3883 2099 1642 1140 865 653 298 161 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3868 7025 7414 9404 882 Size: 617 2369 3157 389 1990 2219 Fragments arranged by size: 3157 2369 2219 1990 617 389 BsaAI yAC'GTr Cuts at: 493 1204 2932 3321 3726 3775 8532 493 Size: 711 1728 389 405 49 4757 2702 Fragments arranged by size: 4757 2702 1728 711 405 389 49 BsaBI GATnn'nnATC Cuts at: 10735 10735 Size: 10741 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4329 8548 8930 9565 Size: 53 83 186 936 2796 4219 382 635 Cuts at: 9565 275 Size: 1451 Fragments arranged by size: 4219 2796 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2615 4896 9174 9572 9846 9993 10404 2615 Size: 2281 4278 398 274 147 411 2952 Fragments arranged by size: 4278 2952 2281 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2051 2081 3710 3740 4524 Size: 30 165 30 648 30 1629 30 784 Cuts at: 4524 4554 4847 4877 5511 5541 6709 6739 7609 Size: 30 293 30 634 30 1168 30 870 Cuts at: 7609 7638 7639 7668 1178 Size: 29 1 29 4251 Fragments arranged by size: 4251 1629 1168 870 784 648 634 293 165 30 30 30 30 30 30 30 30 29 29 1 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2210 2440 6025 6871 7417 7849 7858 1186 Size: 1024 230 3585 846 546 432 9 4069 Fragments arranged by size: 4069 3585 1024 846 546 432 230 9 BseYI C'CCAG_C Cuts at: 2662 2717 4190 4464 4916 5413 6296 7217 7939 Size: 55 1473 274 452 497 883 921 722 Cuts at: 7939 9944 10440 2662 Size: 2005 496 2963 Fragments arranged by size: 2963 2005 1473 921 883 722 497 496 452 274 55 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3218 3439 4414 5216 5844 1163 Size: 573 1482 221 975 802 628 6060 Fragments arranged by size: 6060 1482 975 802 628 573 221 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 7181 8191 8952 9980 1540 Size: 275 186 5180 1010 761 1028 2301 Fragments arranged by size: 5180 2301 1028 1010 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3962 4044 4929 6840 8802 8887 9958 3962 Size: 82 885 1911 1962 85 1071 4745 Fragments arranged by size: 4745 1962 1911 1071 885 85 82 BsmI GAATG_Cn' Cuts at: 3522 4772 6981 7213 8252 8345 3522 Size: 1250 2209 232 1039 93 5918 Fragments arranged by size: 5918 2209 1250 1039 232 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2482 2615 5514 2482 Size: 133 2899 7709 Fragments arranged by size: 7709 2899 133 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2139 2379 2757 3962 4044 4659 4917 Size: 399 349 240 378 1205 82 615 258 Cuts at: 4917 4929 5268 6380 6429 6783 6840 7000 7155 Size: 12 339 1112 49 354 57 160 155 Cuts at: 7155 7224 7839 8060 8802 8887 9958 1391 Size: 69 615 221 742 85 1071 2174 Fragments arranged by size: 2174 1205 1112 1071 742 615 615 399 378 354 349 339 258 240 221 160 155 85 82 69 57 49 12 BspEI T'CCGG_A Cuts at: 10404 10404 Size: 10741 BspHI T'CATG_A Cuts at: 6918 8630 10360 6918 Size: 1712 1730 7299 Fragments arranged by size: 7299 1730 1712 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3190 3639 4016 6624 7602 8096 844 Size: 2346 449 377 2608 978 494 3489 Fragments arranged by size: 3489 2608 2346 978 494 449 377 BsrBI CCG'CTC Cuts at: 1394 1823 3185 8628 1394 Size: 429 1362 5443 3507 Fragments arranged by size: 5443 3507 1362 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2425 3058 4252 6081 9236 9410 65 Size: 1183 1177 633 1194 1829 3155 174 1396 Fragments arranged by size: 3155 1829 1396 1194 1183 1177 633 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3271 3906 5213 9385 9572 1700 Size: 110 1461 635 1307 4172 187 2869 Fragments arranged by size: 4172 2869 1461 1307 635 187 110 BsrGI T'GTAC_A Cuts at: 96 2560 96 Size: 2464 8277 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 10741 BssSI C'ACGA_G Cuts at: 3955 7873 8801 9813 3955 Size: 3918 928 1012 4883 Fragments arranged by size: 4883 3918 1012 928 BstAPI GCAn_nnn'nTGC Cuts at: 794 4561 6970 794 Size: 3767 2409 4565 Fragments arranged by size: 4565 3767 2409 BstBI TT'CG_AA Cuts at: 9545 9545 Size: 10741 BstEII G'GTnAC_C Cuts at: 1184 3931 4148 5072 6793 7691 1184 Size: 2747 217 924 1721 898 4234 Fragments arranged by size: 4234 2747 1721 924 898 217 BstXI CCAn_nnnn'nTGG Cuts at: 4081 4528 5401 7831 7846 4081 Size: 447 873 2430 15 6976 Fragments arranged by size: 6976 2430 873 447 15 BstYI r'GATC_y Cuts at: 1972 2199 2217 2513 5541 6101 8071 8438 8823 Size: 227 18 296 3028 560 1970 367 385 Cuts at: 8823 8840 10292 10378 10390 10736 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 3028 1977 1970 1452 560 385 367 346 296 227 86 18 17 12 Bsu36I CC'TnA_GG Cuts at: 4968 6415 7638 4968 Size: 1447 1223 8071 Fragments arranged by size: 8071 1447 1223 BtgI C'CryG_G Cuts at: 513 1065 2121 2187 2535 2712 4091 4627 4722 Size: 552 1056 66 348 177 1379 536 95 Cuts at: 4722 5056 5580 5613 5897 7050 7824 7905 513 Size: 334 524 33 284 1153 774 81 3349 Fragments arranged by size: 3349 1379 1153 1056 774 552 536 524 348 334 284 177 95 81 66 33 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2687 4382 487 Size: 683 56 1461 1695 6846 Fragments arranged by size: 6846 1695 1461 683 56 BtsI GCAGTG_nn' Cuts at: 3456 5827 6278 7617 7976 8257 9051 9071 3456 Size: 2371 451 1339 359 281 794 20 5126 Fragments arranged by size: 5126 2371 1339 794 451 359 281 20 ClaI AT'CG_AT Cuts at: 1554 2608 1554 Size: 1054 9687 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4828 4863 529 Size: 35 4264 35 6407 Fragments arranged by size: 6407 4264 35 35 DraI TTT'AAA Cuts at: 6120 8045 6120 Size: 1925 8816 DraIII CAC_nnn'GTG Cuts at: 7512 7512 Size: 10741 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3443 9748 809 Size: 507 2127 6305 1802 Fragments arranged by size: 6305 2127 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3904 3908 4225 Size: 54 1425 50 275 18 2074 4 317 Cuts at: 4225 4398 4836 7178 7446 7685 7908 8188 9077 Size: 173 438 2342 268 239 223 280 889 Cuts at: 9077 10706 8 Size: 1629 43 Fragments arranged by size: 2342 2074 1629 1425 889 438 317 280 275 268 239 223 173 54 50 43 18 4 EagI C'GGCC_G Cuts at: 1537 1812 7178 8188 1537 Size: 275 5366 1010 4090 Fragments arranged by size: 5366 4090 1010 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4530 5803 7008 7470 7645 8671 1353 Size: 3177 1273 1205 462 175 1026 3423 Fragments arranged by size: 3423 3177 1273 1205 1026 462 175 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2210 3780 4955 6022 7852 9328 Size: 1064 138 621 1570 1175 1067 1830 1476 Cuts at: 9328 9702 9848 387 Size: 374 146 1280 Fragments arranged by size: 1830 1570 1476 1280 1175 1067 1064 621 374 146 138 EcoICRI GAG'CTC Cuts at: 3960 4927 3960 Size: 967 9774 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2053 2260 2310 2801 3403 5003 5351 Size: 393 421 207 50 491 602 1600 348 Cuts at: 5351 5474 5784 5860 6228 6924 7327 7567 7835 Size: 123 310 76 368 696 403 240 268 Cuts at: 7835 7853 8804 9401 10188 10456 1239 Size: 18 951 597 787 268 1524 Fragments arranged by size: 1600 1524 951 787 696 602 597 491 421 403 393 368 348 310 268 268 240 207 123 76 50 18 EcoNI CCTnn'n_nnAGG Cuts at: 3488 4301 3488 Size: 813 9928 EcoO109I rG'GnC_Cy Cuts at: 3512 5222 5573 5938 6546 6582 7152 7452 7765 Size: 1710 351 365 608 36 570 300 313 Cuts at: 7765 3512 Size: 6488 Fragments arranged by size: 6488 1710 608 570 365 351 313 300 36 EcoRI G'AATT_C Cuts at: 5361 5745 8050 8428 5361 Size: 384 2305 378 7674 Fragments arranged by size: 7674 2305 384 378 EcoRV GAT'ATC Cuts at: 4852 4852 Size: 10741 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 5368 5368 Size: 10741 FseI GG_CCGG'CC Cuts at: 3910 3910 Size: 10741 FspI TGC'GCA Cuts at: 3167 9247 3167 Size: 6080 4661 HaeII r_GCGC'y Cuts at: 1678 2643 2750 7822 9888 1678 Size: 965 107 5072 2066 2531 Fragments arranged by size: 5072 2531 2066 965 107 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2002 2034 2051 2083 2511 Size: 32 135 32 430 32 17 32 428 Cuts at: 2511 2543 3164 3196 3577 3609 3961 3993 4534 Size: 32 621 32 381 32 352 32 541 Cuts at: 4534 4566 4976 5008 5511 5543 5749 5781 5971 Size: 32 410 32 503 32 206 32 190 Cuts at: 5971 6003 6559 6591 7031 7063 7096 7128 7517 Size: 32 556 32 440 32 33 32 389 Cuts at: 7517 7549 9382 9414 9456 9488 1373 Size: 32 1833 32 42 32 2626 Fragments arranged by size: 2626 1833 621 556 541 503 440 430 428 410 389 381 352 206 190 135 42 33 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 17 HincII GTy'rAC Cuts at: 669 1657 1938 5125 7062 7545 8085 8331 669 Size: 988 281 3187 1937 483 540 246 3079 Fragments arranged by size: 3187 3079 1937 988 540 483 281 246 HindIII A'AGCT_T Cuts at: 748 2272 6031 748 Size: 1524 3759 5458 Fragments arranged by size: 5458 3759 1524 HpaI GTT'AAC Cuts at: 8331 8331 Size: 10741 KpnI G_GTAC'C Cuts at: 2182 3049 8066 2182 Size: 867 5017 4857 Fragments arranged by size: 5017 4857 867 MfeI C'AATT_G Cuts at: 4132 4212 8340 4132 Size: 80 4128 6533 Fragments arranged by size: 6533 4128 80 MluI A'CGCG_T Cuts at: 6475 10617 6475 Size: 4142 6599 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2916 3366 9855 10039 1305 Size: 1611 450 6489 184 2007 Fragments arranged by size: 6489 2007 1611 450 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4400 7448 7910 10 Size: 54 1425 343 2568 3048 462 2841 Fragments arranged by size: 3048 2841 2568 1425 462 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3154 3579 4880 5495 6476 7844 8699 Size: 648 1988 425 1301 615 981 1368 855 Cuts at: 8699 9058 9217 518 Size: 359 159 2042 Fragments arranged by size: 2042 1988 1368 1301 981 855 648 615 425 359 159 MspA1I CmG'CkG Cuts at: 1606 2315 2592 2775 3453 3838 4034 4464 4828 Size: 709 277 183 678 385 196 430 364 Cuts at: 4828 5133 5582 5597 6078 7924 8157 8834 9982 Size: 305 449 15 481 1846 233 677 1148 Cuts at: 9982 10227 1606 Size: 245 2120 Fragments arranged by size: 2120 1846 1148 709 678 677 481 449 430 385 364 305 277 245 233 196 183 15 NaeI GCC'GGC Cuts at: 3908 5215 3908 Size: 1307 9434 NcoI C'CATG_G Cuts at: 513 1065 2712 4722 5613 5897 513 Size: 552 1647 2010 891 284 5357 Fragments arranged by size: 5357 2010 1647 891 552 284 NdeI CA'TA_TG Cuts at: 387 387 Size: 10741 NgoMIV G'CCGG_C Cuts at: 3906 5213 3906 Size: 1307 9434 NheI G'CTAG_C Cuts at: 1052 1052 Size: 10741 NotI GC'GGCC_GC Cuts at: 8188 8188 Size: 10741 NruI TCG'CGA Cuts at: 2042 2042 Size: 10741 NsiI A_TGCA'T Cuts at: 2092 2092 Size: 10741 NspI r_CATG'y Cuts at: 1132 1229 2794 4271 4885 7788 8099 9644 1132 Size: 97 1565 1477 614 2903 311 1545 2229 Fragments arranged by size: 2903 2229 1565 1545 1477 614 311 97 PasI CC'CwG_GG Cuts at: 1348 3917 6380 6993 7323 1348 Size: 2569 2463 613 330 4766 Fragments arranged by size: 4766 2569 2463 613 330 PciI A'CATG_T Cuts at: 9640 9640 Size: 10741 PflMI CCAn_nnn'nTGG Cuts at: 1108 3612 3917 4170 5500 6564 7251 7305 1108 Size: 2504 305 253 1330 1064 687 54 4544 Fragments arranged by size: 4544 2504 1330 1064 687 305 253 54 PfoI T'CCnGG_A Cuts at: 3432 7830 3432 Size: 4398 6343 PmeI GTTT'AAAC Cuts at: 8045 8045 Size: 10741 PmlI CAC'GTG Cuts at: 3321 3726 3775 3321 Size: 405 49 10287 Fragments arranged by size: 10287 405 49 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6416 6448 8810 8842 10323 10355 6416 Size: 32 2362 32 1481 32 6802 Fragments arranged by size: 6802 2362 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 5573 5938 6582 7765 5573 Size: 365 644 1183 8549 Fragments arranged by size: 8549 1183 644 365 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 10741 PsiI TTA'TAA Cuts at: 3116 8311 3116 Size: 5195 5546 PspOMI G'GGCC_C Cuts at: 6376 7151 7220 6376 Size: 775 69 9897 Fragments arranged by size: 9897 775 69 PstI C_TGCA'G Cuts at: 830 3011 4557 5953 6249 6621 6645 7333 7613 Size: 2181 1546 1396 296 372 24 688 280 Cuts at: 7613 8093 830 Size: 480 3478 Fragments arranged by size: 3478 2181 1546 1396 688 480 372 296 280 24 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 10741 PvuII CAG'CTG Cuts at: 2315 4828 6078 2315 Size: 2513 1250 6978 Fragments arranged by size: 6978 2513 1250 SacI G_AGCT'C Cuts at: 3962 4929 3962 Size: 967 9774 SacII CC_GC'GG Cuts at: 5583 5583 Size: 10741 SalI G'TCGA_C Cuts at: 1936 5123 8083 1936 Size: 3187 2960 4594 Fragments arranged by size: 4594 3187 2960 SanDI GG'GwC_CC Cuts at: 5938 5938 Size: 10741 SapI GCTCTTCn'nnn_ Cuts at: 7470 7470 Size: 10741 SbfI CC_TGCA'GG Cuts at: 6621 8093 6621 Size: 1472 9269 ScaI AGT'ACT Cuts at: 6251 8989 10701 6251 Size: 2738 1712 6291 Fragments arranged by size: 6291 2738 1712 SexAI A'CCwGG_T Cuts at: 6629 6629 Size: 10741 SfcI C'TryA_G Cuts at: 826 1045 3007 4553 4870 5485 5740 5949 6245 Size: 219 1962 1546 317 615 255 209 296 Cuts at: 6245 6617 6641 7329 7609 8089 9224 9905 10096 Size: 372 24 688 280 480 1135 681 191 Cuts at: 10096 826 Size: 1471 Fragments arranged by size: 1962 1546 1471 1135 688 681 615 480 372 317 296 280 255 219 209 191 24 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 10741 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 10741 SmaI CCC'GGG Cuts at: 1872 2215 7991 8068 1872 Size: 343 5776 77 4545 Fragments arranged by size: 5776 4545 343 77 SmlI C'TyrA_G Cuts at: 820 1017 1943 3186 3347 3964 5255 5642 6782 Size: 197 926 1243 161 617 1291 387 1140 Cuts at: 6782 8845 9746 10008 820 Size: 2063 901 262 1553 Fragments arranged by size: 2063 1553 1291 1243 1140 926 901 617 387 262 197 161 SnaBI TAC'GTA Cuts at: 493 8532 493 Size: 8039 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 10741 SphI G_CATG'C Cuts at: 1132 7788 8099 1132 Size: 6656 311 3774 Fragments arranged by size: 6656 3774 311 SspI AAT'ATT Cuts at: 5 52 3946 8665 5 Size: 47 3894 4719 2081 Fragments arranged by size: 4719 3894 2081 47 StuI AGG'CCT Cuts at: 1517 5201 5720 1517 Size: 3684 519 6538 Fragments arranged by size: 6538 3684 519 StyI C'CwwG_G Cuts at: 513 1065 2712 3925 4521 4722 5343 5613 5897 Size: 552 1647 1213 596 201 621 270 284 Cuts at: 5897 7212 8180 513 Size: 1315 968 3074 Fragments arranged by size: 3074 1647 1315 1213 968 621 596 552 284 270 201 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4494 5405 5773 5998 7336 8768 8785 Size: 1255 2914 911 368 225 1338 1432 17 Cuts at: 8785 8938 9123 9568 325 Size: 153 185 445 1498 Fragments arranged by size: 2914 1498 1432 1338 1255 911 445 368 225 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2560 3720 5082 Size: 274 80 33 51 1443 583 1160 1362 Cuts at: 5082 5475 5667 5772 6130 6249 6540 6716 7563 Size: 393 192 105 358 119 291 176 847 Cuts at: 7563 8987 10699 96 Size: 1424 1712 138 Fragments arranged by size: 1712 1443 1424 1362 1160 847 583 393 358 291 274 192 176 138 119 105 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2944 2976 3066 3098 3710 3742 4524 4556 4847 Size: 32 90 32 612 32 782 32 291 Cuts at: 4847 4879 5041 5073 7609 7641 10701 10733 2944 Size: 32 162 32 2536 32 3060 32 2952 Fragments arranged by size: 3060 2952 2536 782 612 291 162 90 32 32 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 5525 5912 6175 1316 Size: 4209 387 263 5882 Fragments arranged by size: 5882 4209 387 263 XbaI T'CTAG_A Cuts at: 2516 8077 2516 Size: 5561 5180 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2128 2772 4525 2011 Size: 117 644 1753 8227 Fragments arranged by size: 8227 1753 644 117 XhoI C'TCGA_G Cuts at: 1943 5255 1943 Size: 3312 7429 XmaI C'CCGG_G Cuts at: 1870 2213 7989 8066 1870 Size: 343 5776 77 4545 Fragments arranged by size: 5776 4545 343 77 XmnI GAAnn'nnTTC Cuts at: 2048 5171 8870 2048 Size: 3123 3699 3919 Fragments arranged by size: 3919 3699 3123 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4330 8549 276 Size: 53 83 186 936 2796 4219 2468 Fragments arranged by size: 4219 2796 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BsiWI BstZ17I FspAI KasI NarI PacI PspXI PsrI RsrII SfiI SfoI SrfI SwaI