(Circular) (Six-base) MAPSORT of: pFN21AB3598.seq Check: 7636 from: 1 to: 9202 pFN21AB3598 9202 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3691 7012 278 Size: 53 83 186 936 2155 3321 2468 Fragments arranged by size: 3321 2468 2155 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 6545 1937 Size: 4608 4594 Acc65I G'GTAC_C Cuts at: 3317 5171 6523 3317 Size: 1854 1352 5996 Fragments arranged by size: 5996 1854 1352 AclI AA'CG_TT Cuts at: 1563 7329 7702 1563 Size: 5766 373 3063 Fragments arranged by size: 5766 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2918 3216 3639 3774 4386 5438 5538 5781 Size: 1286 298 423 135 612 1052 100 243 Cuts at: 5781 5840 7265 8649 8917 1632 Size: 59 1425 1384 268 1917 Fragments arranged by size: 1917 1425 1384 1286 1052 612 423 298 268 243 135 100 59 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 9005 AflIII A'CryG_T Cuts at: 1203 5888 6008 8101 9078 1203 Size: 4685 120 2093 977 1327 Fragments arranged by size: 4685 2093 1327 977 120 AgeI A'CCGG_T Cuts at: 8033 8033 Size: 9202 AhdI GACnn_n'nnGTC Cuts at: 7931 7931 Size: 9202 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 9202 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2715 1184 Size: 1531 7671 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3112 3144 3526 3558 4696 4728 3112 Size: 32 382 32 1138 32 7586 Fragments arranged by size: 7586 1138 382 32 32 32 AlwNI CAG_nnn'CTG Cuts at: 3599 4769 4787 5633 6945 8517 3599 Size: 1170 18 846 1312 1572 4284 Fragments arranged by size: 4284 1572 1312 1170 846 18 ApaI G_GGCC'C Cuts at: 2780 3231 3977 4418 5112 5642 2780 Size: 451 746 441 694 530 6340 Fragments arranged by size: 6340 746 694 530 451 441 ApaLI G'TGCA_C Cuts at: 3075 7259 8415 3075 Size: 4184 1156 3862 Fragments arranged by size: 4184 3862 1156 ApoI r'AATT_y Cuts at: 1493 2320 3390 6467 6511 6737 6889 8998 1493 Size: 827 1070 3077 44 226 152 2109 1697 Fragments arranged by size: 3077 2109 1697 1070 827 226 152 44 AseI AT'TA_AT Cuts at: 160 1032 7756 160 Size: 872 6724 1606 Fragments arranged by size: 6724 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9202 AvaI C'yCGr_G Cuts at: 1870 1943 3717 3836 4464 4491 4509 4808 5319 Size: 73 1774 119 628 27 18 299 511 Cuts at: 5319 6527 1870 Size: 1208 4545 Fragments arranged by size: 4545 1774 1208 628 511 299 119 73 27 18 AvrII C'CTAG_G Cuts at: 4798 4798 Size: 9202 BamHI G'GATC_C Cuts at: 3679 4649 6532 6899 3679 Size: 970 1883 367 5982 Fragments arranged by size: 5982 1883 970 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2993 3290 3317 5171 5252 Size: 325 217 627 1206 297 27 1854 81 Cuts at: 5252 6523 7978 618 Size: 1271 1455 1842 Fragments arranged by size: 1854 1842 1455 1271 1206 627 325 297 217 81 27 BanII G_rGCy'C Cuts at: 1391 2780 2863 2881 3231 3311 3934 3977 4418 Size: 1389 83 18 350 80 623 43 441 Cuts at: 4418 4780 5112 5642 6521 1391 Size: 362 332 530 879 4072 Fragments arranged by size: 4072 1389 879 623 530 441 362 350 332 83 80 43 18 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2097 2461 2879 3248 4274 4391 4823 Size: 971 198 364 418 369 1026 117 432 Cuts at: 4823 5444 5453 928 Size: 621 9 4677 Fragments arranged by size: 4677 1026 971 621 432 418 369 364 198 117 9 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3004 3038 7393 7427 1903 Size: 34 1067 34 4355 34 3678 Fragments arranged by size: 4355 3678 1067 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3114 6227 7094 8304 3114 Size: 3113 867 1210 4012 Fragments arranged by size: 4012 3113 1210 867 BclI T'GATC_A Cuts at: 1548 4084 5398 1548 Size: 2536 1314 5352 Fragments arranged by size: 5352 2536 1314 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 3306 3855 Size: 107 122 71 631 605 337 1297 549 Cuts at: 3855 4233 4485 5034 5439 5584 5664 5862 7813 Size: 378 252 549 405 145 80 198 1951 Cuts at: 7813 136 Size: 1525 Fragments arranged by size: 1951 1525 1297 631 605 549 549 405 378 337 252 198 145 122 107 80 71 BglII A'GATC_T Cuts at: 2184 6430 9197 2184 Size: 4246 2767 2189 Fragments arranged by size: 4246 2767 2189 BlpI GC'TnA_GC Cuts at: 3337 6619 3337 Size: 3282 5920 Bme1580I G_kGCm'C Cuts at: 1790 2780 3079 3231 3977 4418 5112 5257 5642 Size: 990 299 152 746 441 694 145 385 Cuts at: 5642 5721 7263 8419 1790 Size: 79 1542 1156 2573 Fragments arranged by size: 2573 1542 1156 990 746 694 441 385 299 152 145 79 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 9202 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2026 3234 5537 Size: 416 53 83 399 24 609 1208 2303 Cuts at: 5537 6933 7891 442 Size: 1396 958 1753 Fragments arranged by size: 2303 1753 1396 1208 958 609 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9202 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2228 3948 2228 Size: 1720 7482 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2325 2397 2505 3234 3342 3531 3792 3912 Size: 1086 72 108 729 108 189 261 120 Cuts at: 3912 3930 3999 4035 4083 4119 4227 4281 4404 Size: 18 69 36 48 36 108 54 123 Cuts at: 4404 4461 4551 4596 4614 4746 4764 4794 4809 Size: 57 90 45 18 132 18 30 15 Cuts at: 4809 5268 5520 5583 7862 1239 Size: 459 252 63 2279 2579 Fragments arranged by size: 2579 2279 1086 729 459 261 252 189 132 123 120 108 108 108 90 72 69 63 57 54 48 45 36 36 30 18 18 18 15 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2584 3612 7327 8192 8490 2584 Size: 1028 3715 865 298 3296 Fragments arranged by size: 3715 3296 1028 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5211 5400 6117 6123 7865 882 Size: 617 3712 189 717 6 1742 2219 Fragments arranged by size: 3712 2219 1742 717 617 189 6 BsaAI yAC'GTr Cuts at: 493 1204 2387 2765 5889 6041 6993 493 Size: 711 1183 378 3124 152 952 2702 Fragments arranged by size: 3124 2702 1183 952 711 378 152 BsaBI GATnn'nnATC Cuts at: 9196 9196 Size: 9202 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3688 7009 7391 8026 Size: 53 83 186 936 2155 3321 382 635 Cuts at: 8026 275 Size: 1451 Fragments arranged by size: 3321 2155 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6327 7635 8033 8307 8454 8865 6327 Size: 1308 398 274 147 411 6664 Fragments arranged by size: 6664 1308 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2701 2731 3295 3325 1178 Size: 30 165 30 1298 30 564 30 7055 Fragments arranged by size: 7055 1298 564 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2027 2448 4014 4149 4176 5319 1186 Size: 841 421 1566 135 27 1143 5069 Fragments arranged by size: 5069 1566 1143 841 421 135 27 BseYI C'CCAG_C Cuts at: 2591 5038 5348 5878 8405 8901 2591 Size: 2447 310 530 2527 496 2892 Fragments arranged by size: 2892 2527 2447 530 496 310 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2110 2721 1163 Size: 573 374 611 7644 Fragments arranged by size: 7644 611 573 374 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4971 6652 7413 8441 1540 Size: 275 186 2970 1681 761 1028 2301 Fragments arranged by size: 2970 2301 1681 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2521 2863 2953 3061 3079 3574 3628 3718 3934 Size: 342 90 108 18 495 54 90 216 Cuts at: 3934 4780 5284 7263 7348 8419 2521 Size: 846 504 1979 85 1071 3304 Fragments arranged by size: 3304 1979 1071 846 504 495 342 216 108 90 90 85 54 18 BsmI GAATG_Cn' Cuts at: 2234 2282 3027 6713 6806 2234 Size: 48 745 3686 93 4630 Fragments arranged by size: 4630 3686 745 93 48 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2521 2780 2863 2881 2953 3061 3079 Size: 399 731 259 83 18 72 108 18 Cuts at: 3079 3231 3311 3574 3628 3718 3934 3977 4418 Size: 152 80 263 54 90 216 43 441 Cuts at: 4418 4780 5112 5257 5284 5642 5721 6521 7263 Size: 362 332 145 27 358 79 800 742 Cuts at: 7263 7348 8419 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 800 742 731 441 399 362 358 332 263 259 216 152 145 108 90 85 83 80 79 72 54 43 27 18 18 BspEI T'CCGG_A Cuts at: 8865 8865 Size: 9202 BspHI T'CATG_A Cuts at: 7091 8821 7091 Size: 1730 7472 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2179 3161 4421 4795 5222 6557 844 Size: 1335 982 1260 374 427 1335 3489 Fragments arranged by size: 3489 1335 1335 1260 982 427 374 BsrBI CCG'CTC Cuts at: 1394 1823 7089 1394 Size: 429 5266 3507 Fragments arranged by size: 5266 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5170 7697 7871 65 Size: 1183 3922 2527 174 1396 Fragments arranged by size: 3922 2527 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2401 7846 8033 1700 Size: 110 591 5445 187 2869 Fragments arranged by size: 5445 2869 591 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 9202 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9202 BssSI C'ACGA_G Cuts at: 2466 2862 5382 7262 8274 2466 Size: 396 2520 1880 1012 3394 Fragments arranged by size: 3394 2520 1880 1012 396 BstAPI GCAn_nnn'nTGC Cuts at: 794 3905 794 Size: 3111 6091 BstBI TT'CG_AA Cuts at: 8006 8006 Size: 9202 BstEII G'GTnAC_C Cuts at: 1184 2903 3524 3875 1184 Size: 1719 621 351 6511 Fragments arranged by size: 6511 1719 621 351 BstYI r'GATC_y Cuts at: 1972 2184 2849 3353 3679 4102 4649 5308 5740 Size: 212 665 504 326 423 547 659 432 Cuts at: 5740 5957 6430 6532 6899 7284 7301 8753 8839 Size: 217 473 102 367 385 17 1452 86 Cuts at: 8839 8851 9197 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 665 659 547 504 473 432 423 385 367 346 326 217 212 102 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2475 3204 3366 3384 5634 2475 Size: 729 162 18 2250 6043 Fragments arranged by size: 6043 2250 729 162 18 BtgI C'CryG_G Cuts at: 513 1065 2997 513 Size: 552 1932 6718 Fragments arranged by size: 6718 1932 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 8463 Fragments arranged by size: 8463 683 56 BtsI GCAGTG_nn' Cuts at: 3877 5620 6718 7512 7532 3877 Size: 1743 1098 794 20 5547 Fragments arranged by size: 5547 1743 1098 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9202 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9167 DraI TTT'AAA Cuts at: 6506 6506 Size: 9202 DraIII CAC_nnn'GTG Cuts at: 3056 3497 3056 Size: 441 8761 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2619 2748 2919 3108 4557 4827 5151 Size: 507 1303 129 171 189 1449 270 324 Cuts at: 5151 5340 8209 809 Size: 189 2869 1802 Fragments arranged by size: 2869 1802 1449 1303 507 324 270 189 189 171 129 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2137 3793 5683 Size: 54 1425 50 275 18 307 1656 1890 Cuts at: 5683 6649 7538 9167 8 Size: 966 889 1629 43 Fragments arranged by size: 1890 1656 1629 1425 966 889 307 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6649 1537 Size: 275 4837 4090 Fragments arranged by size: 4837 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 7132 1353 Size: 5779 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2036 4704 7789 8163 8309 387 Size: 1064 138 447 2668 3085 374 146 1280 Fragments arranged by size: 3085 2668 1280 1064 447 374 146 138 EcoICRI GAG'CTC Cuts at: 2861 3932 4778 2861 Size: 1071 846 7285 Fragments arranged by size: 7285 1071 846 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2325 2397 2505 2918 3216 3234 3342 Size: 393 693 72 108 413 298 18 108 Cuts at: 3342 3531 3639 3774 3792 3912 3930 3999 4035 Size: 189 108 135 18 120 18 69 36 Cuts at: 4035 4083 4119 4227 4281 4386 4404 4461 4551 Size: 48 36 108 54 105 18 57 90 Cuts at: 4551 4596 4614 4746 4764 4794 4809 5268 5438 Size: 45 18 132 18 30 15 459 170 Cuts at: 5438 5520 5538 5583 5781 5840 7265 7862 8649 Size: 82 18 45 198 59 1425 597 787 Cuts at: 8649 8917 1239 Size: 268 1524 Fragments arranged by size: 1524 1425 787 693 597 459 413 393 298 268 198 189 170 135 132 120 108 108 108 108 105 90 82 72 69 59 57 54 48 45 45 36 36 30 18 18 18 18 18 18 18 15 EcoNI CCTnn'n_nnAGG Cuts at: 4065 5952 4065 Size: 1887 7315 EcoO109I rG'GnC_Cy Cuts at: 2408 2525 2552 2651 2705 2714 2776 2777 2930 Size: 117 27 99 54 9 62 1 153 Cuts at: 2930 3002 3110 3173 3191 3200 3228 3344 3362 Size: 72 108 63 18 9 28 116 18 Cuts at: 3362 3515 3641 3659 3749 3803 3857 4010 4019 Size: 153 126 18 90 54 54 153 9 Cuts at: 4019 4055 4127 4254 4406 4415 4433 4559 4766 Size: 36 72 127 152 9 18 126 207 Cuts at: 4766 4784 4793 4820 4840 4912 5108 5109 5324 Size: 18 9 27 20 72 196 1 215 Cuts at: 5324 5378 5531 5630 5638 5639 5675 2408 Size: 54 153 99 8 1 36 5935 Fragments arranged by size: 5935 215 207 196 153 153 153 153 152 127 126 126 117 116 108 99 99 90 72 72 72 63 62 54 54 54 54 36 36 28 27 27 20 18 18 18 18 18 9 9 9 9 9 8 1 1 1 EcoRI G'AATT_C Cuts at: 3390 6467 6511 6889 3390 Size: 3077 44 378 5703 Fragments arranged by size: 5703 3077 378 44 EcoRV GAT'ATC Cuts at: 5710 6301 5710 Size: 591 8611 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3779 6403 3779 Size: 2624 6578 FspI TGC'GCA Cuts at: 5852 7708 5852 Size: 1856 7346 HaeII r_GCGC'y Cuts at: 1678 8349 1678 Size: 6671 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2022 2054 2591 2623 3718 Size: 32 135 32 450 32 537 32 1095 Cuts at: 3718 3750 4832 4864 6122 6154 6338 6370 7843 Size: 32 1082 32 1258 32 184 32 1473 Cuts at: 7843 7875 7917 7949 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1473 1258 1095 1082 537 450 184 135 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2510 6546 6792 669 Size: 988 281 572 4036 246 3079 Fragments arranged by size: 4036 3079 988 572 281 246 HindIII A'AGCT_T Cuts at: 748 5903 748 Size: 5155 4047 HpaI GTT'AAC Cuts at: 6792 6792 Size: 9202 KpnI G_GTAC'C Cuts at: 3321 5175 6527 3321 Size: 1854 1352 5996 Fragments arranged by size: 5996 1854 1352 MfeI C'AATT_G Cuts at: 5589 6801 5589 Size: 1212 7990 MluI A'CGCG_T Cuts at: 9078 9078 Size: 9202 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2577 2926 4735 5176 8316 8500 1305 Size: 1272 349 1809 441 3140 184 2007 Fragments arranged by size: 3140 2007 1809 1272 441 349 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2139 5685 10 Size: 54 1425 343 307 3546 3527 Fragments arranged by size: 3546 3527 1425 343 307 54 MslI CAynn'nnrTG Cuts at: 518 1166 4857 5724 7160 7519 7678 518 Size: 648 3691 867 1436 359 159 2042 Fragments arranged by size: 3691 2042 1436 867 648 359 159 MspA1I CmG'CkG Cuts at: 1606 4376 4880 4961 5042 5159 5696 6618 7295 Size: 2770 504 81 81 117 537 922 677 Cuts at: 7295 8443 8688 1606 Size: 1148 245 2120 Fragments arranged by size: 2770 2120 1148 922 677 537 504 245 117 81 81 NcoI C'CATG_G Cuts at: 513 1065 2997 513 Size: 552 1932 6718 Fragments arranged by size: 6718 1932 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 9202 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9202 NotI GC'GGCC_GC Cuts at: 6649 6649 Size: 9202 NspI r_CATG'y Cuts at: 1132 1229 4996 5023 5737 6012 6560 8105 1132 Size: 97 3767 27 714 275 548 1545 2229 Fragments arranged by size: 3767 2229 1545 714 548 275 97 27 PasI CC'CwG_GG Cuts at: 1348 2270 3881 4555 5222 5573 5801 1348 Size: 922 1611 674 667 351 228 4749 Fragments arranged by size: 4749 1611 922 674 667 351 228 PciI A'CATG_T Cuts at: 6008 8101 6008 Size: 2093 7109 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 9202 PfoI T'CCnGG_A Cuts at: 2395 2413 2521 2530 2737 2854 3061 3142 3151 Size: 18 108 9 207 117 207 81 9 Cuts at: 3151 3349 3358 3412 3682 3934 4060 4096 4105 Size: 198 9 54 270 252 126 36 9 Cuts at: 4105 4627 4681 4780 5284 2395 Size: 522 54 99 504 6313 Fragments arranged by size: 6313 522 504 270 252 207 207 198 126 117 108 99 81 54 54 36 18 9 9 9 9 PmeI GTTT'AAAC Cuts at: 6506 6506 Size: 9202 PmlI CAC'GTG Cuts at: 2765 5889 2765 Size: 3124 6078 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7271 7303 8784 8816 7271 Size: 32 1481 32 7657 Fragments arranged by size: 7657 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2408 2525 2552 2651 2705 2714 3002 3110 3173 Size: 117 27 99 54 9 288 108 63 Cuts at: 3173 3200 3362 3515 3641 3659 3749 3803 4010 Size: 27 162 153 126 18 90 54 207 Cuts at: 4010 4127 4254 4406 4433 4559 4784 4793 4820 Size: 117 127 152 27 126 225 9 27 Cuts at: 4820 4840 4912 5324 5378 5531 5630 5675 2408 Size: 20 72 412 54 153 99 45 5935 Fragments arranged by size: 5935 412 288 225 207 162 153 153 152 127 126 126 117 117 108 99 99 90 72 63 54 54 54 45 27 27 27 27 20 18 9 9 PshAI GACnn'nnGTC Cuts at: 1613 2539 5421 1613 Size: 926 2882 5394 Fragments arranged by size: 5394 2882 926 PsiI TTA'TAA Cuts at: 6772 6772 Size: 9202 PspOMI G'GGCC_C Cuts at: 2776 3227 3973 4414 5108 5638 2776 Size: 451 746 441 694 530 6340 Fragments arranged by size: 6340 746 694 530 451 441 PspXI vC'TCGA_Gb Cuts at: 4464 4464 Size: 9202 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3265 3297 6194 6226 3265 Size: 32 2897 32 6241 Fragments arranged by size: 6241 2897 32 32 PstI C_TGCA'G Cuts at: 830 5233 6554 830 Size: 4403 1321 3478 Fragments arranged by size: 4403 3478 1321 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9202 PvuII CAG'CTG Cuts at: 4376 4880 4961 5159 5696 4376 Size: 504 81 198 537 7882 Fragments arranged by size: 7882 537 504 198 81 SacI G_AGCT'C Cuts at: 2863 3934 4780 2863 Size: 1071 846 7285 Fragments arranged by size: 7285 1071 846 SalI G'TCGA_C Cuts at: 1936 6544 1936 Size: 4608 4594 SanDI GG'GwC_CC Cuts at: 4254 4793 4840 4912 5675 4254 Size: 539 47 72 763 7781 Fragments arranged by size: 7781 763 539 72 47 SbfI CC_TGCA'GG Cuts at: 5233 6554 5233 Size: 1321 7881 ScaI AGT'ACT Cuts at: 7450 9162 7450 Size: 1712 7490 SexAI A'CCwGG_T Cuts at: 2494 2512 2728 2953 3124 4078 4276 4456 4933 Size: 18 216 225 171 954 198 180 477 Cuts at: 4933 5338 6047 2494 Size: 405 709 5649 Fragments arranged by size: 5649 954 709 477 405 225 216 198 180 171 18 SfcI C'TryA_G Cuts at: 826 1045 2709 3177 3645 4725 5130 5229 5328 Size: 219 1664 468 468 1080 405 99 99 Cuts at: 5328 6550 7685 8366 8557 826 Size: 1222 1135 681 191 1471 Fragments arranged by size: 1664 1471 1222 1135 1080 681 468 468 405 219 191 99 99 SfiI GGCCn_nnn'nGGCC Cuts at: 3855 5034 5584 5664 3855 Size: 1179 550 80 7393 Fragments arranged by size: 7393 1179 550 80 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9202 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9202 SmaI CCC'GGG Cuts at: 1872 3838 4493 4810 6529 1872 Size: 1966 655 317 1719 4545 Fragments arranged by size: 4545 1966 1719 655 317 SmlI C'TyrA_G Cuts at: 820 1017 1943 2563 3627 4464 4509 7306 8207 Size: 197 926 620 1064 837 45 2797 901 Cuts at: 8207 8469 820 Size: 262 1553 Fragments arranged by size: 2797 1553 1064 926 901 837 620 262 197 45 SnaBI TAC'GTA Cuts at: 493 6993 493 Size: 6500 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 9202 SphI G_CATG'C Cuts at: 1132 4996 5023 5737 6560 1132 Size: 3864 27 714 823 3774 Fragments arranged by size: 3864 3774 823 714 27 SspI AAT'ATT Cuts at: 5 52 6319 7126 5 Size: 47 6267 807 2081 Fragments arranged by size: 6267 2081 807 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 9202 StyI C'CwwG_G Cuts at: 513 1065 2663 2961 2997 4329 4708 4798 5374 Size: 552 1598 298 36 1332 379 90 576 Cuts at: 5374 5953 6641 513 Size: 579 688 3074 Fragments arranged by size: 3074 1598 1332 688 579 576 552 379 298 90 36 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2562 2981 3764 5267 5424 5892 7229 Size: 1255 982 419 783 1503 157 468 1337 Cuts at: 7229 7246 7399 7584 8029 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1503 1498 1337 1255 982 783 468 445 419 185 157 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2145 7448 9160 Size: 274 80 33 51 1443 168 5303 1712 Cuts at: 9160 96 Size: 138 Fragments arranged by size: 5303 1712 1443 274 168 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 9162 9194 9162 Size: 32 9170 Tth111I GACn'n_nGTC Cuts at: 1316 4215 1316 Size: 2899 6303 XbaI T'CTAG_A Cuts at: 6538 6538 Size: 9202 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2986 3004 4715 5381 6045 2986 Size: 18 1711 666 664 6143 Fragments arranged by size: 6143 1711 666 664 18 XhoI C'TCGA_G Cuts at: 1943 4464 4509 1943 Size: 2521 45 6636 Fragments arranged by size: 6636 2521 45 XmaI C'CCGG_G Cuts at: 1870 3836 4491 4808 6527 1870 Size: 1966 655 317 1719 4545 Fragments arranged by size: 4545 1966 1719 655 317 XmnI GAAnn'nnTTC Cuts at: 7331 7331 Size: 9202 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3689 7010 276 Size: 53 83 186 936 2155 3321 2468 Fragments arranged by size: 3321 2468 2155 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI RsrII SacII SapI SfoI SrfI SwaI