(Circular) (Six-base) MAPSORT of: pFN21AB3497.seq Check: 4334 from: 1 to: 9016 pFN21AB3497 9016 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 5371 5371 Size: 9016 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6826 278 Size: 53 83 186 936 5290 2468 Fragments arranged by size: 5290 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3173 6359 1937 Size: 1236 3186 4594 Fragments arranged by size: 4594 3186 1236 Acc65I G'GTAC_C Cuts at: 2419 6337 2419 Size: 3918 5098 AclI AA'CG_TT Cuts at: 1563 5143 7143 7516 1563 Size: 3580 2000 373 3063 Fragments arranged by size: 3580 3063 2000 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2148 2649 3222 3515 3711 4005 5106 7079 Size: 516 501 573 293 196 294 1101 1973 Cuts at: 7079 8463 8731 1632 Size: 1384 268 1917 Fragments arranged by size: 1973 1917 1384 1101 573 516 501 294 293 268 196 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8819 AflIII A'CryG_T Cuts at: 1203 3492 7915 8892 1203 Size: 2289 4423 977 1327 Fragments arranged by size: 4423 2289 1327 977 AgeI A'CCGG_T Cuts at: 7847 7847 Size: 9016 AhdI GACnn_n'nnGTC Cuts at: 7745 7745 Size: 9016 AleI CACnn'nnGTG Cuts at: 1166 3372 5704 1166 Size: 2206 2332 4478 Fragments arranged by size: 4478 2332 2206 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3390 4844 4954 5857 1184 Size: 2206 1454 110 903 4343 Fragments arranged by size: 4343 2206 1454 903 110 AlwNI CAG_nnn'CTG Cuts at: 4028 6759 8331 4028 Size: 2731 1572 4713 Fragments arranged by size: 4713 2731 1572 ApaI G_GGCC'C Cuts at: 6184 6184 Size: 9016 ApaLI G'TGCA_C Cuts at: 7073 8229 7073 Size: 1156 7860 ApoI r'AATT_y Cuts at: 1493 2349 2373 3342 3554 4319 4346 5510 6140 Size: 856 24 969 212 765 27 1164 630 Cuts at: 6140 6325 6551 6703 8812 1493 Size: 185 226 152 2109 1697 Fragments arranged by size: 2109 1697 1164 969 856 765 630 226 212 185 152 27 24 AseI AT'TA_AT Cuts at: 160 1032 5537 7570 160 Size: 872 4505 2033 1606 Fragments arranged by size: 4505 2033 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9016 AvaI C'yCGr_G Cuts at: 1870 1943 2919 3150 6341 1870 Size: 73 976 231 3191 4545 Fragments arranged by size: 4545 3191 976 231 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2975 3008 2975 Size: 33 8983 BamHI G'GATC_C Cuts at: 6346 6713 6346 Size: 367 8649 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2419 2915 3860 6337 7792 Size: 325 217 627 632 496 945 2477 1455 Cuts at: 7792 618 Size: 1842 Fragments arranged by size: 2477 1842 1455 945 632 627 496 325 217 BanII G_rGCy'C Cuts at: 1391 5578 6184 6335 1391 Size: 4187 606 151 4072 Fragments arranged by size: 4187 4072 606 151 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2450 2505 3848 928 Size: 971 551 55 1343 6096 Fragments arranged by size: 6096 1343 971 551 55 BbvCI CC'TCA_GC Cuts at: 3027 4389 3027 Size: 1362 7654 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 7207 7241 1903 Size: 34 5270 34 3678 Fragments arranged by size: 5270 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2232 2289 4534 6908 8118 2232 Size: 57 2245 2374 1210 3130 Fragments arranged by size: 3130 2374 2245 1210 57 BclI T'GATC_A Cuts at: 1548 1548 Size: 9016 BfrBI ATG'CAT Cuts at: 5962 5962 Size: 9016 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 7627 136 Size: 107 122 71 631 605 5955 1525 Fragments arranged by size: 5955 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2197 2624 3272 4935 9011 2197 Size: 427 648 1663 4076 2202 Fragments arranged by size: 4076 2202 1663 648 427 BlpI GC'TnA_GC Cuts at: 6433 6433 Size: 9016 Bme1580I G_kGCm'C Cuts at: 1790 2920 4793 5390 6184 7077 8233 1790 Size: 1130 1873 597 794 893 1156 2573 Fragments arranged by size: 2573 1873 1156 1130 893 794 597 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 9016 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2744 4254 5041 Size: 416 53 83 399 24 1327 1510 787 Cuts at: 5041 6747 7705 442 Size: 1706 958 1753 Fragments arranged by size: 1753 1706 1510 1327 958 787 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9016 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3337 3337 Size: 9016 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3483 4205 4509 5415 6280 7676 1239 Size: 2244 722 304 906 865 1396 2579 Fragments arranged by size: 2579 2244 1396 906 865 722 304 Bpu10I CC'TnA_GC Cuts at: 3027 4389 4817 3027 Size: 1362 428 7226 Fragments arranged by size: 7226 1362 428 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2906 3316 5188 7141 8006 8304 2906 Size: 410 1872 1953 865 298 3618 Fragments arranged by size: 3618 1953 1872 865 410 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2520 7679 882 Size: 617 1021 5159 2219 Fragments arranged by size: 5159 2219 1021 617 BsaAI yAC'GTr Cuts at: 493 1204 6807 493 Size: 711 5603 2702 Fragments arranged by size: 5603 2702 711 BsaBI GATnn'nnATC Cuts at: 4201 9010 4201 Size: 4809 4207 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6823 7205 7840 275 Size: 53 83 186 936 5290 382 635 1451 Fragments arranged by size: 5290 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 7449 7847 8121 8268 8679 7449 Size: 398 274 147 411 7786 Fragments arranged by size: 7786 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 5988 6018 6077 6107 6291 Size: 30 165 30 4585 30 59 30 184 Cuts at: 6291 6321 1178 Size: 30 3873 Fragments arranged by size: 4585 3873 184 165 59 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2400 3313 1186 Size: 1214 913 6889 Fragments arranged by size: 6889 1214 913 BseYI C'CCAG_C Cuts at: 2182 2880 4792 5389 5472 8219 8715 2182 Size: 698 1912 597 83 2747 496 2483 Fragments arranged by size: 2747 2483 1912 698 597 496 83 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2328 4073 5279 6133 6141 6180 1163 Size: 573 592 1745 1206 854 8 39 3999 Fragments arranged by size: 3999 1745 1206 854 592 573 39 8 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4509 6466 7227 8255 1540 Size: 275 186 2508 1957 761 1028 2301 Fragments arranged by size: 2508 2301 1957 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2559 3103 7077 7162 8233 2559 Size: 544 3974 85 1071 3342 Fragments arranged by size: 3974 3342 1071 544 85 BsmI GAATG_Cn' Cuts at: 3541 4817 5375 5558 6527 6620 3541 Size: 1276 558 183 969 93 5937 Fragments arranged by size: 5937 1276 969 558 183 93 BsmBI CGTCTCn'nnnn_ Cuts at: 5923 5923 Size: 9016 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2559 2920 3103 4793 5390 5578 6184 Size: 399 769 361 183 1690 597 188 606 Cuts at: 6184 6335 7077 7162 8233 1391 Size: 151 742 85 1071 2174 Fragments arranged by size: 2174 1690 1071 769 742 606 597 399 361 188 183 151 85 BspEI T'CCGG_A Cuts at: 8679 8679 Size: 9016 BspHI T'CATG_A Cuts at: 2971 4327 5020 5667 6169 6905 8635 2971 Size: 1356 693 647 502 736 1730 3352 Fragments arranged by size: 3352 1730 1356 736 693 647 502 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3802 3979 3993 5371 6371 844 Size: 2958 177 14 1378 1000 3489 Fragments arranged by size: 3489 2958 1378 1000 177 14 BsrBI CCG'CTC Cuts at: 1394 1823 5464 6903 1394 Size: 429 3641 1439 3507 Fragments arranged by size: 3641 3507 1439 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2121 3117 4500 7511 7685 65 Size: 1183 873 996 1383 3011 174 1396 Fragments arranged by size: 3011 1396 1383 1183 996 873 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5639 7660 7847 1700 Size: 110 3829 2021 187 2869 Fragments arranged by size: 3829 2869 2021 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 9016 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9016 BssSI C'ACGA_G Cuts at: 5579 7076 8088 5579 Size: 1497 1012 6507 Fragments arranged by size: 6507 1497 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 5909 794 Size: 5115 3901 BstBI TT'CG_AA Cuts at: 7820 7820 Size: 9016 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 9016 BstXI CCAn_nnnn'nTGG Cuts at: 6258 6258 Size: 9016 BstYI r'GATC_y Cuts at: 1972 2197 2624 3272 4935 5262 6346 6713 7098 Size: 225 427 648 1663 327 1084 367 385 Cuts at: 7098 7115 8567 8653 8665 9011 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1663 1452 1084 648 427 385 367 346 327 225 86 17 12 BstZ17I GTA'TAC Cuts at: 3174 3174 Size: 9016 Bsu36I CC'TnA_GG Cuts at: 3828 5480 3828 Size: 1652 7364 BtgI C'CryG_G Cuts at: 513 1065 2004 4059 6092 6234 513 Size: 552 939 2055 2033 142 3295 Fragments arranged by size: 3295 2055 2033 939 552 142 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 6120 487 Size: 683 56 4894 3383 Fragments arranged by size: 4894 3383 683 56 BtsI GCAGTG_nn' Cuts at: 4353 4393 5851 6124 6532 7326 7346 4353 Size: 40 1458 273 408 794 20 6023 Fragments arranged by size: 6023 1458 794 408 273 40 20 ClaI AT'CG_AT Cuts at: 1554 3561 1554 Size: 2007 7009 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3763 3798 529 Size: 35 3199 35 5747 Fragments arranged by size: 5747 3199 35 35 DraI TTT'AAA Cuts at: 2535 6320 2535 Size: 3785 5231 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 8023 809 Size: 507 6707 1802 Fragments arranged by size: 6707 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2137 4506 4925 Size: 54 1425 50 275 18 307 2369 419 Cuts at: 4925 5864 6463 7352 8981 8 Size: 939 599 889 1629 43 Fragments arranged by size: 2369 1629 1425 939 889 599 419 307 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4506 6463 1537 Size: 275 2694 1957 4090 Fragments arranged by size: 4090 2694 1957 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2625 3198 3375 3381 3534 4075 5889 5921 Size: 1272 573 177 6 153 541 1814 32 Cuts at: 5921 6946 1353 Size: 1025 3423 Fragments arranged by size: 3423 1814 1272 1025 573 541 177 153 32 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2068 7603 7977 8123 387 Size: 1064 138 479 5535 374 146 1280 Fragments arranged by size: 5535 1280 1064 479 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2148 2649 3222 3483 3515 3711 4005 Size: 393 516 501 573 261 32 196 294 Cuts at: 4005 4205 4509 5106 5415 6280 7079 7676 8463 Size: 200 304 597 309 865 799 597 787 Cuts at: 8463 8731 1239 Size: 268 1524 Fragments arranged by size: 1524 865 799 787 597 597 573 516 501 393 309 304 294 268 261 200 196 32 EcoO109I rG'GnC_Cy Cuts at: 4972 5476 6180 6181 4972 Size: 504 704 1 7807 Fragments arranged by size: 7807 704 504 1 EcoRI G'AATT_C Cuts at: 6140 6325 6703 6140 Size: 185 378 8453 Fragments arranged by size: 8453 378 185 EcoRV GAT'ATC Cuts at: 4426 4426 Size: 9016 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3647 3647 Size: 9016 FspI TGC'GCA Cuts at: 7522 7522 Size: 9016 HaeII r_GCGC'y Cuts at: 1678 8163 1678 Size: 6485 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2624 2656 2690 2722 3197 Size: 32 135 32 1052 32 34 32 475 Cuts at: 3197 3229 4040 4072 5550 5582 5922 5954 5988 Size: 32 811 32 1478 32 340 32 34 Cuts at: 5988 6020 7657 7689 7731 7763 1373 Size: 32 1637 32 42 32 2626 Fragments arranged by size: 2626 1637 1478 1052 811 475 340 135 42 34 34 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2698 5610 5775 5886 6360 6606 Size: 988 281 760 2912 165 111 474 246 Cuts at: 6606 669 Size: 3079 Fragments arranged by size: 3079 2912 988 760 474 281 246 165 111 HindIII A'AGCT_T Cuts at: 748 5077 748 Size: 4329 4687 HpaI GTT'AAC Cuts at: 5610 5775 6606 5610 Size: 165 831 8020 Fragments arranged by size: 8020 831 165 KpnI G_GTAC'C Cuts at: 2423 6341 2423 Size: 3918 5098 MfeI C'AATT_G Cuts at: 6615 6615 Size: 9016 MluI A'CGCG_T Cuts at: 8892 8892 Size: 9016 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2801 8130 8314 1305 Size: 1496 5329 184 2007 Fragments arranged by size: 5329 2007 1496 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2139 4927 10 Size: 54 1425 343 307 2788 4099 Fragments arranged by size: 4099 2788 1425 343 307 54 MslI CAynn'nnrTG Cuts at: 518 1166 3372 3455 4091 4229 4826 5704 6974 Size: 648 2206 83 636 138 597 878 1270 Cuts at: 6974 7333 7492 518 Size: 359 159 2042 Fragments arranged by size: 2206 2042 1270 878 648 636 597 359 159 138 83 MspA1I CmG'CkG Cuts at: 1606 4383 5295 5312 5393 6125 6432 7109 8257 Size: 2777 912 17 81 732 307 677 1148 Cuts at: 8257 8502 1606 Size: 245 2120 Fragments arranged by size: 2777 2120 1148 912 732 677 307 245 81 17 NaeI GCC'GGC Cuts at: 5641 5641 Size: 9016 NcoI C'CATG_G Cuts at: 513 1065 2004 4059 6234 513 Size: 552 939 2055 2175 3295 Fragments arranged by size: 3295 2175 2055 939 552 NdeI CA'TA_TG Cuts at: 387 2149 4657 6073 387 Size: 1762 2508 1416 3330 Fragments arranged by size: 3330 2508 1762 1416 NgoMIV G'CCGG_C Cuts at: 5639 5639 Size: 9016 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9016 NotI GC'GGCC_GC Cuts at: 6463 6463 Size: 9016 NsiI A_TGCA'T Cuts at: 5964 5964 Size: 9016 NspI r_CATG'y Cuts at: 1132 1229 3496 4519 5880 6374 7919 1132 Size: 97 2267 1023 1361 494 1545 2229 Fragments arranged by size: 2267 2229 1545 1361 1023 494 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 9016 PciI A'CATG_T Cuts at: 3492 7915 3492 Size: 4423 4593 PflMI CCAn_nnn'nTGG Cuts at: 1108 6074 1108 Size: 4966 4050 PfoI T'CCnGG_A Cuts at: 5148 5148 Size: 9016 PmeI GTTT'AAAC Cuts at: 6320 6320 Size: 9016 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7085 7117 8598 8630 7085 Size: 32 1481 32 7471 Fragments arranged by size: 7471 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 9016 PsiI TTA'TAA Cuts at: 3635 5787 6586 3635 Size: 2152 799 6065 Fragments arranged by size: 6065 2152 799 PspOMI G'GGCC_C Cuts at: 6180 6180 Size: 9016 PstI C_TGCA'G Cuts at: 830 3990 4033 5764 5846 6368 830 Size: 3160 43 1731 82 522 3478 Fragments arranged by size: 3478 3160 1731 522 82 43 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9016 PvuII CAG'CTG Cuts at: 5312 5393 6125 5312 Size: 81 732 8203 Fragments arranged by size: 8203 732 81 SalI G'TCGA_C Cuts at: 1936 6358 1936 Size: 4422 4594 SapI GCTCTTCn'nnn_ Cuts at: 2625 3381 3534 2625 Size: 756 153 8107 Fragments arranged by size: 8107 756 153 SbfI CC_TGCA'GG Cuts at: 3990 5764 6368 3990 Size: 1774 604 6638 Fragments arranged by size: 6638 1774 604 ScaI AGT'ACT Cuts at: 2733 3337 7264 8976 2733 Size: 604 3927 1712 2773 Fragments arranged by size: 3927 2773 1712 604 SfcI C'TryA_G Cuts at: 826 1045 3540 3708 3834 3986 4029 4543 5760 Size: 219 2495 168 126 152 43 514 1217 Cuts at: 5760 5842 6364 7499 8180 8371 826 Size: 82 522 1135 681 191 1471 Fragments arranged by size: 2495 1471 1217 1135 681 522 514 219 191 168 152 126 82 43 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9016 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9016 SmaI CCC'GGG Cuts at: 1872 6343 1872 Size: 4471 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2885 3150 3331 5203 7120 8021 Size: 197 926 942 265 181 1872 1917 901 Cuts at: 8021 8283 820 Size: 262 1553 Fragments arranged by size: 1917 1872 1553 942 926 901 265 262 197 181 SnaBI TAC'GTA Cuts at: 493 6807 493 Size: 6314 2702 SpeI A'CTAG_T Cuts at: 152 3932 5096 152 Size: 3780 1164 4072 Fragments arranged by size: 4072 3780 1164 SphI G_CATG'C Cuts at: 1132 6374 1132 Size: 5242 3774 SspI AAT'ATT Cuts at: 5 52 6940 5 Size: 47 6888 2081 Fragments arranged by size: 6888 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 9016 StyI C'CwwG_G Cuts at: 513 1065 2004 2717 2832 2849 4059 5619 6234 Size: 552 939 713 115 17 1210 1560 615 Cuts at: 6234 6455 513 Size: 221 3074 Fragments arranged by size: 3074 1560 1210 939 713 615 552 221 115 17 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4269 7043 7060 7213 7398 7843 325 Size: 1255 2689 2774 17 153 185 445 1498 Fragments arranged by size: 2774 2689 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2542 2731 3335 Size: 274 80 33 51 1443 565 189 604 Cuts at: 3335 7262 8974 96 Size: 3927 1712 138 Fragments arranged by size: 3927 1712 1443 604 565 274 189 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2634 2666 4950 4982 5327 5359 6077 6109 8976 Size: 32 2284 32 345 32 718 32 2867 Cuts at: 8976 9008 2634 Size: 32 2642 Fragments arranged by size: 2867 2642 2284 718 345 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 9016 XbaI T'CTAG_A Cuts at: 3968 6352 3968 Size: 2384 6632 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2961 4839 6078 2961 Size: 1878 1239 5899 Fragments arranged by size: 5899 1878 1239 XhoI C'TCGA_G Cuts at: 1943 3150 1943 Size: 1207 7809 XmaI C'CCGG_G Cuts at: 1870 6341 1870 Size: 4471 4545 XmnI GAAnn'nnTTC Cuts at: 2199 3754 3822 5656 7145 2199 Size: 1555 68 1834 1489 4070 Fragments arranged by size: 4070 1834 1555 1489 68 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6824 276 Size: 53 83 186 936 5290 2468 Fragments arranged by size: 5290 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BbeI BsiWI DraIII EcoICRI EcoNI FseI FspAI KasI NarI NruI PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI