Restriction map for
pFN21AB3497
(Linear) (Six-base) MAPSORT of: KIBB3497.seq Check: 8075 from: 1 to: 4311
KIBB3497.orf
With 182 enzymes: SgfI *
October 24, 2006 15:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3366 4311
Size: 3366 945
AccI GT'mk_AC
Cuts at: 0 1168 4311
Size: 1168 3143
Acc65I G'GTAC_C
Cuts at: 0 414 4311
Size: 414 3897
AclI AA'CG_TT
Cuts at: 0 3138 4311
Size: 3138 1173
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 143 644 1217 1510 1706 2000 3101 4311
Size: 143 501 573 293 196 294 1101 1210
Fragments arranged by size:
1210 1101 573 501 294 293 196 143
AflIII A'CryG_T
Cuts at: 0 1487 4311
Size: 1487 2824
AleI CACnn'nnGTG
Cuts at: 0 1367 3699 4311
Size: 1367 2332 612
Fragments arranged by size:
2332 1367 612
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1385 2839 2949 3852 4311
Size: 1385 1454 110 903 459
Fragments arranged by size:
1454 1385 903 459 110
AlwNI CAG_nnn'CTG
Cuts at: 0 2023 4311
Size: 2023 2288
ApaI G_GGCC'C
Cuts at: 0 4179 4311
Size: 4179 132
ApoI r'AATT_y
Cuts at: 0 344 368 1337 1549 2314 2341 3505 4135
Size: 344 24 969 212 765 27 1164 630
Cuts at: 4135 4311
Size: 176
Fragments arranged by size:
1164 969 765 630 344 212 176 27
24
AseI AT'TA_AT
Cuts at: 0 3532 4311
Size: 3532 779
AvaI C'yCGr_G
Cuts at: 0 914 1145 4311
Size: 914 231 3166
Fragments arranged by size:
3166 914 231
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 970 1003 4311
Size: 970 33 3308
Fragments arranged by size:
3308 970 33
BanI G'GyrC_C
Cuts at: 0 414 910 1855 4311
Size: 414 496 945 2456
Fragments arranged by size:
2456 945 496 414
BanII G_rGCy'C
Cuts at: 0 3573 4179 4311
Size: 3573 606 132
Fragments arranged by size:
3573 606 132
BbsI GAAGACnn'nnnn_
Cuts at: 0 445 500 1843 4311
Size: 445 55 1343 2468
Fragments arranged by size:
2468 1343 445 55
BbvCI CC'TCA_GC
Cuts at: 0 1022 2384 4311
Size: 1022 1362 1927
Fragments arranged by size:
1927 1362 1022
BciVI GTATCCnnnnn_n'
Cuts at: 0 227 284 2529 4311
Size: 227 57 2245 1782
Fragments arranged by size:
2245 1782 227 57
BfrBI ATG'CAT
Cuts at: 0 3957 4311
Size: 3957 354
BglII A'GATC_T
Cuts at: 0 192 619 1267 2930 4311
Size: 192 427 648 1663 1381
Fragments arranged by size:
1663 1381 648 427 192
Bme1580I G_kGCm'C
Cuts at: 0 915 2788 3385 4179 4311
Size: 915 1873 597 794 132
Fragments arranged by size:
1873 915 794 597 132
BmrI ACTGGGnnnn_n'
Cuts at: 0 739 2249 3036 4311
Size: 739 1510 787 1275
Fragments arranged by size:
1510 1275 787 739
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1332 4311
Size: 1332 2979
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1478 2200 2504 3410 4275 4311
Size: 1478 722 304 906 865 36
Fragments arranged by size:
1478 906 865 722 304 36
Bpu10I CC'TnA_GC
Cuts at: 0 1022 2384 2812 4311
Size: 1022 1362 428 1499
Fragments arranged by size:
1499 1362 1022 428
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 901 1311 3183 4311
Size: 901 410 1872 1128
Fragments arranged by size:
1872 1128 901 410
BsaI GGTCTCn'nnnn_
Cuts at: 0 515 4311
Size: 515 3796
BsaBI GATnn'nnATC
Cuts at: 0 2196 4311
Size: 2196 2115
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 3983 4013 4072 4102 4286 4311
Size: 3983 30 59 30 184 25
Fragments arranged by size:
3983 184 59 30 30 25
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 395 1308 4311
Size: 395 913 3003
Fragments arranged by size:
3003 913 395
BseYI C'CCAG_C
Cuts at: 0 177 875 2787 3384 3467 4311
Size: 177 698 1912 597 83 844
Fragments arranged by size:
1912 844 698 597 177 83
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 323 2068 3274 4128 4136 4175 4311
Size: 323 1745 1206 854 8 39 136
Fragments arranged by size:
1745 1206 854 323 136 39 8
BsiEI CG_ry'CG
Cuts at: 0 2504 4311
Size: 2504 1807
BsiHKAI G_wGCw'C
Cuts at: 0 554 1098 4311
Size: 554 544 3213
Fragments arranged by size:
3213 554 544
BsmI GAATG_Cn'
Cuts at: 0 1536 2812 3370 3553 4311
Size: 1536 1276 558 183 758
Fragments arranged by size:
1536 1276 758 558 183
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3918 4311
Size: 3918 393
Bsp1286I G_dGCh'C
Cuts at: 0 554 915 1098 2788 3385 3573 4179 4311
Size: 554 361 183 1690 597 188 606 132
Fragments arranged by size:
1690 606 597 554 361 188 183 132
BspHI T'CATG_A
Cuts at: 0 966 2322 3015 3662 4164 4311
Size: 966 1356 693 647 502 147
Fragments arranged by size:
1356 966 693 647 502 147
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1797 1974 1988 3366 4311
Size: 1797 177 14 1378 945
Fragments arranged by size:
1797 1378 945 177 14
BsrBI CCG'CTC
Cuts at: 0 3459 4311
Size: 3459 852
BsrDI GCAATG_nn'
Cuts at: 0 116 1112 2495 4311
Size: 116 996 1383 1816
Fragments arranged by size:
1816 1383 996 116
BsrFI r'CCGG_y
Cuts at: 0 3634 4311
Size: 3634 677
BssSI C'ACGA_G
Cuts at: 0 3574 4311
Size: 3574 737
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3904 4311
Size: 3904 407
BstXI CCAn_nnnn'nTGG
Cuts at: 0 4253 4311
Size: 4253 58
BstYI r'GATC_y
Cuts at: 0 192 619 1267 2930 3257 4311
Size: 192 427 648 1663 327 1054
Fragments arranged by size:
1663 1054 648 427 327 192
BstZ17I GTA'TAC
Cuts at: 0 1169 4311
Size: 1169 3142
Bsu36I CC'TnA_GG
Cuts at: 0 1823 3475 4311
Size: 1823 1652 836
Fragments arranged by size:
1823 1652 836
BtgI C'CryG_G
Cuts at: 0 2054 4087 4229 4311
Size: 2054 2033 142 82
Fragments arranged by size:
2054 2033 142 82
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 4115 4311
Size: 4115 196
BtsI GCAGTG_nn'
Cuts at: 0 2348 2388 3846 4119 4311
Size: 2348 40 1458 273 192
Fragments arranged by size:
2348 1458 273 192 40
ClaI AT'CG_AT
Cuts at: 0 1556 4311
Size: 1556 2755
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1758 1793 4311
Size: 1758 35 2518
Fragments arranged by size:
2518 1758 35
DraI TTT'AAA
Cuts at: 0 530 4311
Size: 530 3781
EaeI y'GGCC_r
Cuts at: 0 132 2501 2920 3859 4311
Size: 132 2369 419 939 452
Fragments arranged by size:
2369 939 452 419 132
EagI C'GGCC_G
Cuts at: 0 2501 4311
Size: 2501 1810
EarI CTCTTCn'nnn_
Cuts at: 0 620 1193 1370 1376 1529 2070 3884 3916
Size: 620 573 177 6 153 541 1814 32
Cuts at: 3916 4311
Size: 395
Fragments arranged by size:
1814 620 573 541 395 177 153 32
6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 63 4311
Size: 63 4248
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 143 644 1217 1478 1510 1706 2000 2200
Size: 143 501 573 261 32 196 294 200
Cuts at: 2200 2504 3101 3410 4275 4311
Size: 304 597 309 865 36
Fragments arranged by size:
865 597 573 501 309 304 294 261
200 196 143 36 32
EcoO109I rG'GnC_Cy
Cuts at: 0 2967 3471 4175 4176 4311
Size: 2967 504 704 1 135
Fragments arranged by size:
2967 704 504 135 1
EcoRI G'AATT_C
Cuts at: 0 4135 4311
Size: 4135 176
EcoRV GAT'ATC
Cuts at: 0 2421 4311
Size: 2421 1890
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1642 4311
Size: 1642 2669
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 619 651 685 717 1192 1224 2035 2067
Size: 619 32 34 32 475 32 811 32
Cuts at: 2067 3545 3577 3917 3949 3983 4015 4311
Size: 1478 32 340 32 34 32 296
Fragments arranged by size:
1478 811 619 475 340 296 34 34
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 693 3605 3770 3881 4311
Size: 693 2912 165 111 430
Fragments arranged by size:
2912 693 430 165 111
HindIII A'AGCT_T
Cuts at: 0 3072 4311
Size: 3072 1239
HpaI GTT'AAC
Cuts at: 0 3605 3770 4311
Size: 3605 165 541
Fragments arranged by size:
3605 541 165
KpnI G_GTAC'C
Cuts at: 0 418 4311
Size: 418 3893
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 796 4311
Size: 796 3515
MscI TGG'CCA
Cuts at: 0 134 2922 4311
Size: 134 2788 1389
Fragments arranged by size:
2788 1389 134
MslI CAynn'nnrTG
Cuts at: 0 1367 1450 2086 2224 2821 3699 4311
Size: 1367 83 636 138 597 878 612
Fragments arranged by size:
1367 878 636 612 597 138 83
MspA1I CmG'CkG
Cuts at: 0 2378 3290 3307 3388 4120 4311
Size: 2378 912 17 81 732 191
Fragments arranged by size:
2378 912 732 191 81 17
NaeI GCC'GGC
Cuts at: 0 3636 4311
Size: 3636 675
NcoI C'CATG_G
Cuts at: 0 2054 4229 4311
Size: 2054 2175 82
Fragments arranged by size:
2175 2054 82
NdeI CA'TA_TG
Cuts at: 0 144 2652 4068 4311
Size: 144 2508 1416 243
Fragments arranged by size:
2508 1416 243 144
NgoMIV G'CCGG_C
Cuts at: 0 3634 4311
Size: 3634 677
NsiI A_TGCA'T
Cuts at: 0 3959 4311
Size: 3959 352
NspI r_CATG'y
Cuts at: 0 1491 2514 3875 4311
Size: 1491 1023 1361 436
Fragments arranged by size:
1491 1361 1023 436
PciI A'CATG_T
Cuts at: 0 1487 4311
Size: 1487 2824
PflMI CCAn_nnn'nTGG
Cuts at: 0 4069 4311
Size: 4069 242
PfoI T'CCnGG_A
Cuts at: 0 3143 4311
Size: 3143 1168
PsiI TTA'TAA
Cuts at: 0 1630 3782 4311
Size: 1630 2152 529
Fragments arranged by size:
2152 1630 529
PspOMI G'GGCC_C
Cuts at: 0 4175 4311
Size: 4175 136
PstI C_TGCA'G
Cuts at: 0 1985 2028 3759 3841 4311
Size: 1985 43 1731 82 470
Fragments arranged by size:
1985 1731 470 82 43
PvuII CAG'CTG
Cuts at: 0 3307 3388 4120 4311
Size: 3307 81 732 191
Fragments arranged by size:
3307 732 191 81
SapI GCTCTTCn'nnn_
Cuts at: 0 620 1376 1529 4311
Size: 620 756 153 2782
Fragments arranged by size:
2782 756 620 153
SbfI CC_TGCA'GG
Cuts at: 0 1985 3759 4311
Size: 1985 1774 552
Fragments arranged by size:
1985 1774 552
ScaI AGT'ACT
Cuts at: 0 728 1332 4311
Size: 728 604 2979
Fragments arranged by size:
2979 728 604
SfcI C'TryA_G
Cuts at: 0 1535 1703 1829 1981 2024 2538 3755 3837
Size: 1535 168 126 152 43 514 1217 82
Cuts at: 3837 4311
Size: 474
Fragments arranged by size:
1535 1217 514 474 168 152 126 82
43
SmlI C'TyrA_G
Cuts at: 0 880 1145 1326 3198 4311
Size: 880 265 181 1872 1113
Fragments arranged by size:
1872 1113 880 265 181
SpeI A'CTAG_T
Cuts at: 0 1927 3091 4311
Size: 1927 1164 1220
Fragments arranged by size:
1927 1220 1164
StyI C'CwwG_G
Cuts at: 0 712 827 844 2054 3614 4229 4311
Size: 712 115 17 1210 1560 615 82
Fragments arranged by size:
1560 1210 712 615 115 82 17
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2264 4311
Size: 2264 2047
TatI w'GTAC_w
Cuts at: 0 537 726 1330 4311
Size: 537 189 604 2981
Fragments arranged by size:
2981 604 537 189
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 629 661 2945 2977 3322 3354 4072 4104
Size: 629 32 2284 32 345 32 718 32
Cuts at: 4104 4311
Size: 207
Fragments arranged by size:
2284 718 629 345 207 32 32 32
32
XbaI T'CTAG_A
Cuts at: 0 1963 4311
Size: 1963 2348
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 956 2834 4073 4311
Size: 956 1878 1239 238
Fragments arranged by size:
1878 1239 956 238
XhoI C'TCGA_G
Cuts at: 0 1145 4311
Size: 1145 3166
XmnI GAAnn'nnTTC
Cuts at: 0 194 1749 1817 3651 4311
Size: 194 1555 68 1834 660
Fragments arranged by size:
1834 1555 660 194 68
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AlfI
AlwNI ApaI ApoI AseI AvaI BaeI BanI BanII
BbsI BbvCI BciVI BfrBI BglII Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaBI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI
Hin4I HincII HindIII HpaI KpnI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NsiI NspI PciI
PflMI PfoI PsiI PspOMI PstI PvuII SapI SbfI
ScaI SfcI SmlI SpeI StyI TaqII TatI TstI
XbaI XcmI XhoI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AloI ApaLI AscI
AsiSI AvrII BamHI BbeI BcgI BclI BglI BlpI
BmgBI BmtI BsaAI BsaHI BsaWI BsiWI BspEI BsrGI
BssHII BstBI BstEII DraIII DrdI EcoICRI EcoNI FseI
FspI FspAI HaeII KasI MfeI MluI NarI NheI
NotI NruI PacI PasI PmeI PmlI PpiI PpuMI
PshAI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SphI SrfI SspI StuI SwaI Tth111I XmaI ZraI