(Circular) (Six-base) MAPSORT of: pFN21AB3009.seq Check: 5388 from: 1 to: 6649 pFN21AB3009 6649 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4459 278 Size: 53 83 186 936 2923 2468 Fragments arranged by size: 2923 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3094 3992 1937 Size: 1157 898 4594 Fragments arranged by size: 4594 1157 898 Acc65I G'GTAC_C Cuts at: 3657 3970 3657 Size: 313 6336 AclI AA'CG_TT Cuts at: 1563 2717 4776 5149 1563 Size: 1154 2059 373 3063 Fragments arranged by size: 3063 2059 1154 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3468 3541 3625 3793 3877 4712 6096 6364 Size: 1836 73 84 168 84 835 1384 268 Cuts at: 6364 1632 Size: 1917 Fragments arranged by size: 1917 1836 1384 835 268 168 84 84 73 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6452 AflIII A'CryG_T Cuts at: 1203 5548 6525 1203 Size: 4345 977 1327 Fragments arranged by size: 4345 1327 977 AgeI A'CCGG_T Cuts at: 5480 5480 Size: 6649 AhdI GACnn_n'nnGTC Cuts at: 5378 5378 Size: 6649 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6649 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6649 AlwNI CAG_nnn'CTG Cuts at: 2102 2227 2605 2710 4392 5964 2102 Size: 125 378 105 1682 1572 2787 Fragments arranged by size: 2787 1682 1572 378 125 105 ApaLI G'TGCA_C Cuts at: 4706 5862 4706 Size: 1156 5493 ApoI r'AATT_y Cuts at: 1493 2055 3045 3385 3958 4184 4336 6445 1493 Size: 562 990 340 573 226 152 2109 1697 Fragments arranged by size: 2109 1697 990 573 562 340 226 152 AseI AT'TA_AT Cuts at: 160 1032 5203 160 Size: 872 4171 1606 Fragments arranged by size: 4171 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6649 AvaI C'yCGr_G Cuts at: 1870 1943 2516 2630 3974 1870 Size: 73 573 114 1344 4545 Fragments arranged by size: 4545 1344 573 114 73 BamHI G'GATC_C Cuts at: 2494 3979 4346 2494 Size: 1485 367 4797 Fragments arranged by size: 4797 1485 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2560 3330 3657 3970 5425 Size: 325 217 627 773 770 327 313 1455 Cuts at: 5425 618 Size: 1842 Fragments arranged by size: 1842 1455 773 770 627 327 325 313 217 BanII G_rGCy'C Cuts at: 1391 2549 2598 2663 3968 1391 Size: 1158 49 65 1305 4072 Fragments arranged by size: 4072 1305 1158 65 49 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2817 3680 928 Size: 971 918 863 3897 Fragments arranged by size: 3897 971 918 863 BbvCI CC'TCA_GC Cuts at: 3687 3687 Size: 6649 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4840 4874 1903 Size: 34 2903 34 3678 Fragments arranged by size: 3678 2903 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4541 5751 4541 Size: 1210 5439 BclI T'GATC_A Cuts at: 1548 1548 Size: 6649 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5260 136 Size: 107 122 71 631 605 3588 1525 Fragments arranged by size: 3588 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2551 2862 2998 6644 2551 Size: 311 136 3646 2556 Fragments arranged by size: 3646 2556 311 136 BlpI GC'TnA_GC Cuts at: 4066 4066 Size: 6649 Bme1580I G_kGCm'C Cuts at: 1790 2563 2631 3875 4710 5866 1790 Size: 773 68 1244 835 1156 2573 Fragments arranged by size: 2573 1244 1156 835 773 68 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6649 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2618 3861 4380 Size: 416 53 83 399 24 1201 1243 519 Cuts at: 4380 5338 442 Size: 958 1753 Fragments arranged by size: 1753 1243 1201 958 519 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6649 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2210 2677 2210 Size: 467 6182 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2322 2582 2685 3008 3042 3537 5309 1239 Size: 1083 260 103 323 34 495 1772 2579 Fragments arranged by size: 2579 1772 1083 495 323 260 103 34 Bpu10I CC'TnA_GC Cuts at: 3687 3687 Size: 6649 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2907 3114 3775 4774 5639 5937 2907 Size: 207 661 999 865 298 3619 Fragments arranged by size: 3619 999 865 661 298 207 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2224 2386 3389 5312 882 Size: 617 725 162 1003 1923 2219 Fragments arranged by size: 2219 1923 1003 725 617 162 BsaAI yAC'GTr Cuts at: 493 1204 3870 4440 493 Size: 711 2666 570 2702 Fragments arranged by size: 2702 2666 711 570 BsaBI GATnn'nnATC Cuts at: 6643 6643 Size: 6649 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4456 4838 5473 275 Size: 53 83 186 936 2923 382 635 1451 Fragments arranged by size: 2923 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3378 3669 5082 5480 5754 5901 6312 3378 Size: 291 1413 398 274 147 411 3715 Fragments arranged by size: 3715 1413 411 398 291 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2459 2489 2784 2814 1178 Size: 30 165 30 1056 30 295 30 5013 Fragments arranged by size: 5013 1056 295 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2363 2459 2606 2671 1186 Size: 1177 96 147 65 5164 Fragments arranged by size: 5164 1177 147 96 65 BseYI C'CCAG_C Cuts at: 2316 5852 6348 2316 Size: 3536 496 2617 Fragments arranged by size: 3536 2617 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6076 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4099 4860 5888 1540 Size: 275 186 2098 761 1028 2301 Fragments arranged by size: 2301 2098 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2598 2663 4710 4795 5866 2598 Size: 65 2047 85 1071 3381 Fragments arranged by size: 3381 2047 1071 85 65 BsmI GAATG_Cn' Cuts at: 4160 4253 4160 Size: 93 6556 BsmBI CGTCTCn'nnnn_ Cuts at: 2441 2441 Size: 6649 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2549 2563 2598 2631 2663 3875 3968 Size: 399 759 14 35 33 32 1212 93 Cuts at: 3968 4710 4795 5866 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1212 1071 759 742 399 93 85 35 33 32 14 BspEI T'CCGG_A Cuts at: 6312 6312 Size: 6649 BspHI T'CATG_A Cuts at: 3193 4538 6268 3193 Size: 1345 1730 3574 Fragments arranged by size: 3574 1730 1345 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4004 844 Size: 3160 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2465 4536 1394 Size: 429 642 2071 3507 Fragments arranged by size: 3507 2071 642 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5144 5318 65 Size: 1183 3896 174 1396 Fragments arranged by size: 3896 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2563 5293 5480 1700 Size: 110 753 2730 187 2869 Fragments arranged by size: 2869 2730 753 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6649 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6649 BssSI C'ACGA_G Cuts at: 4709 5721 4709 Size: 1012 5637 BstAPI GCAn_nnn'nTGC Cuts at: 794 3711 794 Size: 2917 3732 BstBI TT'CG_AA Cuts at: 5453 5453 Size: 6649 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6649 BstXI CCAn_nnnn'nTGG Cuts at: 2403 2403 Size: 6649 BstYI r'GATC_y Cuts at: 1972 2035 2256 2494 2551 2862 2998 3979 4346 Size: 63 221 238 57 311 136 981 367 Cuts at: 4346 4731 4748 6200 6286 6298 6644 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 981 385 367 346 311 238 221 136 86 63 57 17 12 Bsu36I CC'TnA_GG Cuts at: 2529 2913 3251 2529 Size: 384 338 5927 Fragments arranged by size: 5927 384 338 BtgI C'CryG_G Cuts at: 513 1065 2004 2016 513 Size: 552 939 12 5146 Fragments arranged by size: 5146 939 552 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5910 Fragments arranged by size: 5910 683 56 BtsI GCAGTG_nn' Cuts at: 2361 4165 4959 4979 2361 Size: 1804 794 20 4031 Fragments arranged by size: 4031 1804 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6649 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3778 3813 529 Size: 35 3214 35 3365 Fragments arranged by size: 3365 3214 35 35 DraI TTT'AAA Cuts at: 3953 3953 Size: 6649 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3827 5656 809 Size: 507 2511 1829 1802 Fragments arranged by size: 2511 1829 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2093 2333 2425 Size: 54 1425 50 275 18 263 240 92 Cuts at: 2425 2730 4096 4985 6614 8 Size: 305 1366 889 1629 43 Fragments arranged by size: 1629 1425 1366 889 305 275 263 240 92 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4096 1537 Size: 275 2284 4090 Fragments arranged by size: 4090 2284 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2043 2583 2691 2877 2973 3387 3797 4579 Size: 690 540 108 186 96 414 410 782 Cuts at: 4579 1353 Size: 3423 Fragments arranged by size: 3423 782 690 540 414 410 186 108 96 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2257 5236 5610 5756 387 Size: 1064 138 668 2979 374 146 1280 Fragments arranged by size: 2979 1280 1064 668 374 146 138 EcoICRI GAG'CTC Cuts at: 2596 2661 2596 Size: 65 6584 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2322 2582 2685 3008 3042 3468 3537 Size: 393 690 260 103 323 34 426 69 Cuts at: 3537 3541 3625 3793 3877 4712 5309 6096 6364 Size: 4 84 168 84 835 597 787 268 Cuts at: 6364 1239 Size: 1524 Fragments arranged by size: 1524 835 787 690 597 426 393 323 268 260 168 103 84 84 69 34 4 EcoNI CCTnn'n_nnAGG Cuts at: 2441 3772 2441 Size: 1331 5318 EcoO109I rG'GnC_Cy Cuts at: 2620 2635 2893 3777 3927 2620 Size: 15 258 884 150 5342 Fragments arranged by size: 5342 884 258 150 15 EcoRI G'AATT_C Cuts at: 2055 3958 4336 2055 Size: 1903 378 4368 Fragments arranged by size: 4368 1903 378 EcoRV GAT'ATC Cuts at: 2902 3905 2902 Size: 1003 5646 FspI TGC'GCA Cuts at: 5155 5155 Size: 6649 HaeII r_GCGC'y Cuts at: 1678 3721 5796 1678 Size: 2043 2075 2531 Fragments arranged by size: 2531 2075 2043 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2459 2491 2536 2568 2668 Size: 32 135 32 887 32 45 32 100 Cuts at: 2668 2700 2784 2816 2849 2881 3796 3828 5290 Size: 32 84 32 33 32 915 32 1462 Cuts at: 5290 5322 5364 5396 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1462 915 887 135 100 84 45 42 33 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3576 3993 4239 669 Size: 988 281 1638 417 246 3079 Fragments arranged by size: 3079 1638 988 417 281 246 HindIII A'AGCT_T Cuts at: 748 3553 3889 748 Size: 2805 336 3508 Fragments arranged by size: 3508 2805 336 HpaI GTT'AAC Cuts at: 3576 4239 3576 Size: 663 5986 KpnI G_GTAC'C Cuts at: 3661 3974 3661 Size: 313 6336 MfeI C'AATT_G Cuts at: 4248 4248 Size: 6649 MluI A'CGCG_T Cuts at: 6525 6525 Size: 6649 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2176 5763 5947 1305 Size: 871 3587 184 2007 Fragments arranged by size: 3587 2007 871 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4827 Fragments arranged by size: 4827 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3868 4607 4966 5125 518 Size: 648 2702 739 359 159 2042 Fragments arranged by size: 2702 2042 739 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3477 4065 4742 5890 6135 1606 Size: 1871 588 677 1148 245 2120 Fragments arranged by size: 2120 1871 1148 677 588 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5158 Fragments arranged by size: 5158 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6649 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6649 NotI GC'GGCC_GC Cuts at: 4096 4096 Size: 6649 NspI r_CATG'y Cuts at: 1132 1229 4007 5552 1132 Size: 97 2778 1545 2229 Fragments arranged by size: 2778 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6649 PciI A'CATG_T Cuts at: 5548 5548 Size: 6649 PflMI CCAn_nnn'nTGG Cuts at: 1108 2342 1108 Size: 1234 5415 PmeI GTTT'AAAC Cuts at: 3953 3953 Size: 6649 PmlI CAC'GTG Cuts at: 3870 3870 Size: 6649 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3684 3716 4718 4750 6231 6263 3684 Size: 32 1002 32 1481 32 4070 Fragments arranged by size: 4070 1481 1002 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2620 2635 2893 3927 2620 Size: 15 258 1034 5342 Fragments arranged by size: 5342 1034 258 15 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6649 PsiI TTA'TAA Cuts at: 4219 4219 Size: 6649 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3344 3376 3344 Size: 32 6617 PstI C_TGCA'G Cuts at: 830 2493 2610 2653 3707 4001 830 Size: 1663 117 43 1054 294 3478 Fragments arranged by size: 3478 1663 1054 294 117 43 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6649 SacI G_AGCT'C Cuts at: 2598 2663 2598 Size: 65 6584 SalI G'TCGA_C Cuts at: 1936 3991 1936 Size: 2055 4594 SanDI GG'GwC_CC Cuts at: 2893 2893 Size: 6649 SapI GCTCTTCn'nnn_ Cuts at: 2691 2877 3797 2691 Size: 186 920 5543 Fragments arranged by size: 5543 920 186 SbfI CC_TGCA'GG Cuts at: 2610 4001 2610 Size: 1391 5258 ScaI AGT'ACT Cuts at: 4897 6609 4897 Size: 1712 4937 SfcI C'TryA_G Cuts at: 826 1045 2010 2309 2489 2606 2649 3445 3703 Size: 219 965 299 180 117 43 796 258 Cuts at: 3703 3997 5132 5813 6004 826 Size: 294 1135 681 191 1471 Fragments arranged by size: 1471 1135 965 796 681 299 294 258 219 191 180 117 43 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6649 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6649 SmaI CCC'GGG Cuts at: 1872 3976 1872 Size: 2104 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2922 3129 3754 4753 5654 5916 Size: 197 926 979 207 625 999 901 262 Cuts at: 5916 820 Size: 1553 Fragments arranged by size: 1553 999 979 926 901 625 262 207 197 SnaBI TAC'GTA Cuts at: 493 4440 493 Size: 3947 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6649 SphI G_CATG'C Cuts at: 1132 4007 1132 Size: 2875 3774 SspI AAT'ATT Cuts at: 5 52 3124 4573 5 Size: 47 3072 1449 2081 Fragments arranged by size: 3072 2081 1449 47 StuI AGG'CCT Cuts at: 1517 3025 1517 Size: 1508 5141 StyI C'CwwG_G Cuts at: 513 1065 2004 2396 2888 4088 513 Size: 552 939 392 492 1200 3074 Fragments arranged by size: 3074 1200 939 552 492 392 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2308 2395 3743 4676 4693 4846 5031 Size: 1255 728 87 1348 933 17 153 185 Cuts at: 5031 5476 325 Size: 445 1498 Fragments arranged by size: 1498 1348 1255 933 728 445 185 153 87 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3536 4895 6607 Size: 274 80 33 51 1443 1559 1359 1712 Cuts at: 6607 96 Size: 138 Fragments arranged by size: 1712 1559 1443 1359 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6609 6641 6609 Size: 32 6617 Tth111I GACn'n_nGTC Cuts at: 1316 3491 1316 Size: 2175 4474 XbaI T'CTAG_A Cuts at: 3985 3985 Size: 6649 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6649 XmaI C'CCGG_G Cuts at: 1870 3974 1870 Size: 2104 4545 XmnI GAAnn'nnTTC Cuts at: 2283 2783 3806 3863 4778 2283 Size: 500 1023 57 915 4154 Fragments arranged by size: 4154 1023 915 500 57 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4457 276 Size: 53 83 186 936 2923 2468 Fragments arranged by size: 2923 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI PstI PvuI SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I DraIII FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspOMI PspXI PvuII RsrII SacII SexAI SfiI SfoI SrfI SwaI XcmI