(Circular) (Six-base) MAPSORT of: pFN21AB1755.seq Check: 9470 from: 1 to: 9100 pFN21AB1755 9100 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3045 3045 Size: 9100 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2189 2838 3884 4107 Size: 53 83 186 936 653 649 1046 223 Cuts at: 4107 6910 278 Size: 2803 2468 Fragments arranged by size: 2803 2468 1046 936 653 649 223 186 83 53 AccI GT'mk_AC Cuts at: 1937 6443 1937 Size: 4506 4594 Acc65I G'GTAC_C Cuts at: 2082 2268 2952 6421 2082 Size: 186 684 3469 4761 Fragments arranged by size: 4761 3469 684 186 AclI AA'CG_TT Cuts at: 1563 7227 7600 1563 Size: 5664 373 3063 Fragments arranged by size: 5664 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2851 2945 3013 4540 4642 5735 5738 7163 Size: 1219 94 68 1527 102 1093 3 1425 Cuts at: 7163 8547 8815 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1527 1425 1384 1219 1093 268 102 94 68 3 AfeI AGC'GCT Cuts at: 2312 2312 Size: 9100 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8903 AflIII A'CryG_T Cuts at: 1203 4482 4704 4815 7999 8976 1203 Size: 3279 222 111 3184 977 1327 Fragments arranged by size: 3279 3184 1327 977 222 111 AgeI A'CCGG_T Cuts at: 3310 3548 7931 3310 Size: 238 4383 4479 Fragments arranged by size: 4479 4383 238 AhdI GACnn_n'nnGTC Cuts at: 4972 7829 4972 Size: 2857 6243 AleI CACnn'nnGTG Cuts at: 1166 3551 1166 Size: 2385 6715 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 9100 AlwNI CAG_nnn'CTG Cuts at: 2214 2278 6843 8415 2214 Size: 64 4565 1572 2899 Fragments arranged by size: 4565 2899 1572 64 ApaI G_GGCC'C Cuts at: 3151 3151 Size: 9100 ApaLI G'TGCA_C Cuts at: 2899 3156 3554 4016 7157 8313 2899 Size: 257 398 462 3141 1156 3686 Fragments arranged by size: 3686 3141 1156 462 398 257 ApoI r'AATT_y Cuts at: 1493 4087 4208 5372 5990 6409 6635 6787 8896 Size: 2594 121 1164 618 419 226 152 2109 Cuts at: 8896 1493 Size: 1697 Fragments arranged by size: 2594 2109 1697 1164 618 419 226 152 121 AseI AT'TA_AT Cuts at: 160 1032 5157 7654 160 Size: 872 4125 2497 1606 Fragments arranged by size: 4125 2497 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9100 AvaI C'yCGr_G Cuts at: 1870 1943 3081 3706 5006 6241 6425 1870 Size: 73 1138 625 1300 1235 184 4545 Fragments arranged by size: 4545 1300 1235 1138 625 184 73 BamHI G'GATC_C Cuts at: 6430 6797 6430 Size: 367 8733 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2082 2268 2952 4739 6421 Size: 325 217 627 295 186 684 1787 1682 Cuts at: 6421 7876 618 Size: 1455 1842 Fragments arranged by size: 1842 1787 1682 1455 684 627 325 295 217 186 BanII G_rGCy'C Cuts at: 1391 2464 3151 3242 3978 4392 4443 5007 5559 Size: 1073 687 91 736 414 51 564 552 Cuts at: 5559 6419 1391 Size: 860 4072 Fragments arranged by size: 4072 1073 860 736 687 564 552 414 91 51 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2154 4648 5298 6089 928 Size: 971 255 2494 650 791 3939 Fragments arranged by size: 3939 2494 971 791 650 255 BbvCI CC'TCA_GC Cuts at: 2318 3590 6385 2318 Size: 1272 2795 5033 Fragments arranged by size: 5033 2795 1272 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2779 2813 5433 5467 6025 6059 7291 Size: 34 842 34 2620 34 558 34 1232 Cuts at: 7291 7325 1903 Size: 34 3678 Fragments arranged by size: 3678 2620 1232 842 558 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3992 6992 8202 3992 Size: 3000 1210 4890 Fragments arranged by size: 4890 3000 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 9100 BfrBI ATG'CAT Cuts at: 5330 5330 Size: 9100 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6389 7711 136 Size: 107 122 71 631 605 4717 1322 1525 Fragments arranged by size: 4717 1525 1322 631 605 122 107 71 BglII A'GATC_T Cuts at: 5814 9095 5814 Size: 3281 5819 BlpI GC'TnA_GC Cuts at: 5118 6164 6517 5118 Size: 1046 353 7701 Fragments arranged by size: 7701 1046 353 Bme1580I G_kGCm'C Cuts at: 1790 2903 3151 3160 3558 4020 7161 8317 1790 Size: 1113 248 9 398 462 3141 1156 2573 Fragments arranged by size: 3141 2573 1156 1113 462 398 248 9 BmgBI CAC'GTC Cuts at: 1318 2965 4485 4707 6061 6295 1318 Size: 1647 1520 222 1354 234 4123 Fragments arranged by size: 4123 1647 1520 1354 234 222 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4395 5425 6831 Size: 416 53 83 399 24 2978 1030 1406 Cuts at: 6831 7789 442 Size: 958 1753 Fragments arranged by size: 2978 1753 1406 1030 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9100 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6193 6193 Size: 9100 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2161 2666 3394 4593 4963 5096 5558 7760 Size: 922 505 728 1199 370 133 462 2202 Cuts at: 7760 1239 Size: 2579 Fragments arranged by size: 2579 2202 1199 922 728 505 462 370 133 Bpu10I CC'TnA_GC Cuts at: 2318 3590 3782 3909 6385 2318 Size: 1272 192 127 2476 5033 Fragments arranged by size: 5033 2476 1272 192 127 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2167 2879 5480 7225 8090 8388 2167 Size: 712 2601 1745 865 298 2879 Fragments arranged by size: 2879 2601 1745 865 712 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3421 4630 4726 7763 882 Size: 617 1922 1209 96 3037 2219 Fragments arranged by size: 3037 2219 1922 1209 617 96 BsaAI yAC'GTr Cuts at: 493 1204 3940 6891 493 Size: 711 2736 2951 2702 Fragments arranged by size: 2951 2736 2702 711 BsaBI GATnn'nnATC Cuts at: 9094 9094 Size: 9100 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2186 2835 2915 3090 Size: 53 83 186 936 653 649 80 175 Cuts at: 3090 3183 3525 3881 4104 6907 7289 7924 275 Size: 93 342 356 223 2803 382 635 1451 Fragments arranged by size: 2803 1451 936 653 649 635 382 356 342 223 186 175 93 83 80 53 BsaWI w'CCGG_w Cuts at: 3310 3548 4918 5960 7533 7931 8205 8352 8763 Size: 238 1370 1042 1573 398 274 147 411 Cuts at: 8763 3310 Size: 3647 Fragments arranged by size: 3647 1573 1370 1042 411 398 274 238 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2495 2525 3788 3818 4163 Size: 30 165 30 1092 30 1263 30 345 Cuts at: 4163 4193 1178 Size: 30 6085 Fragments arranged by size: 6085 1263 1092 345 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2261 2354 3932 4151 4577 4862 5549 1186 Size: 1075 93 1578 219 426 285 687 4737 Fragments arranged by size: 4737 1578 1075 687 426 285 219 93 BseYI C'CCAG_C Cuts at: 3594 3928 4295 6348 8303 8799 3594 Size: 334 367 2053 1955 496 3895 Fragments arranged by size: 3895 2053 1955 496 367 334 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2579 2954 3023 3314 3417 3914 4136 Size: 573 843 375 69 291 103 497 222 Cuts at: 4136 5294 5713 6085 1163 Size: 1158 419 372 4178 Fragments arranged by size: 4178 1158 843 573 497 419 375 372 291 222 103 69 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2159 6550 7311 8339 1540 Size: 275 186 158 4391 761 1028 2301 Fragments arranged by size: 4391 2301 1028 761 275 186 158 BsiHKAI G_wGCw'C Cuts at: 2234 2903 3160 3558 4020 4410 5007 5124 5559 Size: 669 257 398 462 390 597 117 435 Cuts at: 5559 6174 7161 7246 8317 2234 Size: 615 987 85 1071 3017 Fragments arranged by size: 3017 1071 987 669 615 597 462 435 398 390 257 117 85 BsmI GAATG_Cn' Cuts at: 5330 6611 6704 5330 Size: 1281 93 7726 Fragments arranged by size: 7726 1281 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2751 2876 4824 2751 Size: 125 1948 7027 Fragments arranged by size: 7027 1948 125 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2234 2464 2903 3151 3160 3242 3558 Size: 399 444 230 439 248 9 82 316 Cuts at: 3558 3978 4020 4392 4410 4443 5007 5124 5559 Size: 420 42 372 18 33 564 117 435 Cuts at: 5559 6174 6419 7161 7246 8317 1391 Size: 615 245 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 615 564 444 439 435 420 399 372 316 248 245 230 117 85 82 42 33 18 9 BspEI T'CCGG_A Cuts at: 4918 5960 8763 4918 Size: 1042 2803 5255 Fragments arranged by size: 5255 2803 1042 BspHI T'CATG_A Cuts at: 5281 5760 6989 8719 5281 Size: 479 1229 1730 5662 Fragments arranged by size: 5662 1730 1229 479 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2094 2256 3045 3933 4641 6027 6107 6455 Size: 1250 162 789 888 708 1386 80 348 Cuts at: 6455 844 Size: 3489 Fragments arranged by size: 3489 1386 1250 888 789 708 348 162 80 BsrBI CCG'CTC Cuts at: 1394 1823 2449 3628 3663 6987 1394 Size: 429 626 1179 35 3324 3507 Fragments arranged by size: 3507 3324 1179 626 429 35 BsrDI GCAATG_nn' Cuts at: 65 1248 3307 5035 5688 7595 7769 65 Size: 1183 2059 1728 653 1907 174 1396 Fragments arranged by size: 2059 1907 1728 1396 1183 653 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3310 3548 3771 4376 7744 7931 1700 Size: 110 1500 238 223 605 3368 187 2869 Fragments arranged by size: 3368 2869 1500 605 238 223 187 110 BsrGI T'GTAC_A Cuts at: 96 3060 6309 96 Size: 2964 3249 2887 Fragments arranged by size: 3249 2964 2887 BssHII G'CGCG_C Cuts at: 1925 2941 1925 Size: 1016 8084 BssSI C'ACGA_G Cuts at: 4277 7160 8172 4277 Size: 2883 1012 5205 Fragments arranged by size: 5205 2883 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 4676 794 Size: 3882 5218 BstBI TT'CG_AA Cuts at: 7904 7904 Size: 9100 BstEII G'GTnAC_C Cuts at: 1184 2860 6064 1184 Size: 1676 3204 4220 Fragments arranged by size: 4220 3204 1676 BstXI CCAn_nnnn'nTGG Cuts at: 2554 4148 2554 Size: 1594 7506 BstYI r'GATC_y Cuts at: 1972 2469 3701 4396 5814 6327 6430 6797 7182 Size: 497 1232 695 1418 513 103 367 385 Cuts at: 7182 7199 8651 8737 8749 9095 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1418 1232 695 513 497 385 367 346 103 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2553 2707 3162 3613 3651 4029 4884 Size: 552 1488 154 455 451 38 378 855 Cuts at: 4884 5587 513 Size: 703 4026 Fragments arranged by size: 4026 1488 855 703 552 455 451 378 154 38 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2973 487 Size: 683 56 1747 6614 Fragments arranged by size: 6614 1747 683 56 BtsI GCAGTG_nn' Cuts at: 2567 2673 2697 3179 3302 3387 3902 5525 6616 Size: 106 24 482 123 85 515 1623 1091 Cuts at: 6616 7410 7430 2567 Size: 794 20 4237 Fragments arranged by size: 4237 1623 1091 794 515 482 123 106 85 24 20 ClaI AT'CG_AT Cuts at: 1554 2810 5239 1554 Size: 1256 2429 5415 Fragments arranged by size: 5415 2429 1256 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4668 4703 529 Size: 35 4104 35 4926 Fragments arranged by size: 4926 4104 35 35 DraI TTT'AAA Cuts at: 4620 4873 6404 4620 Size: 253 1531 7316 Fragments arranged by size: 7316 1531 253 DraIII CAC_nnn'GTG Cuts at: 4833 4833 Size: 9100 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3137 4816 5168 8107 809 Size: 507 1821 1679 352 2939 1802 Fragments arranged by size: 2939 1821 1802 1679 507 352 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2283 2694 3954 Size: 54 1425 50 275 18 453 411 1260 Cuts at: 3954 4174 4374 6547 7436 9065 8 Size: 220 200 2173 889 1629 43 Fragments arranged by size: 2173 1629 1425 1260 889 453 411 275 220 200 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6547 1537 Size: 275 4735 4090 Fragments arranged by size: 4735 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2030 2439 4995 5343 5847 7030 1353 Size: 677 409 2556 348 504 1183 3423 Fragments arranged by size: 3423 2556 1183 677 504 409 348 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7687 8061 8207 387 Size: 1064 138 6098 374 146 1280 Fragments arranged by size: 6098 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 5005 5557 5005 Size: 552 8548 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2161 2666 2851 2945 3013 3394 4540 Size: 393 529 505 185 94 68 381 1146 Cuts at: 4540 4593 4642 4963 5096 5558 5735 5738 7163 Size: 53 49 321 133 462 177 3 1425 Cuts at: 7163 7760 8547 8815 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1425 1146 787 597 529 505 462 393 381 321 268 185 177 133 94 68 53 49 3 EcoNI CCTnn'n_nnAGG Cuts at: 2869 3041 2869 Size: 172 8928 EcoO109I rG'GnC_Cy Cuts at: 3055 3147 3148 3539 3622 3922 4201 4507 5262 Size: 92 1 391 83 300 279 306 755 Cuts at: 5262 5390 3055 Size: 128 6765 Fragments arranged by size: 6765 755 391 306 300 279 128 92 83 1 EcoRI G'AATT_C Cuts at: 5990 6409 6787 5990 Size: 419 378 8303 Fragments arranged by size: 8303 419 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3016 4924 5322 5462 5623 3016 Size: 1908 398 140 161 6493 Fragments arranged by size: 6493 1908 398 161 140 FspI TGC'GCA Cuts at: 3071 7606 3071 Size: 4535 4565 HaeII r_GCGC'y Cuts at: 1678 2314 3108 8247 1678 Size: 636 794 5139 2531 Fragments arranged by size: 5139 2531 794 636 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2187 2219 2495 2527 2875 Size: 32 135 32 615 32 276 32 348 Cuts at: 2875 2907 3788 3820 4163 4195 4566 4598 4958 Size: 32 881 32 343 32 371 32 360 Cuts at: 4958 4990 5111 5143 6076 6108 7741 7773 7815 Size: 32 121 32 933 32 1633 32 42 Cuts at: 7815 7847 1373 Size: 32 2626 Fragments arranged by size: 2626 1633 933 881 615 371 360 348 343 276 135 121 42 32 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3682 4369 6444 6690 669 Size: 988 281 1744 687 2075 246 3079 Fragments arranged by size: 3079 2075 1744 988 687 281 246 HindIII A'AGCT_T Cuts at: 748 5967 748 Size: 5219 3881 HpaI GTT'AAC Cuts at: 6690 6690 Size: 9100 KpnI G_GTAC'C Cuts at: 2086 2272 2956 6425 2086 Size: 186 684 3469 4761 Fragments arranged by size: 4761 3469 684 186 MfeI C'AATT_G Cuts at: 6699 6699 Size: 9100 MluI A'CGCG_T Cuts at: 4815 8976 4815 Size: 4161 4939 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4689 8214 8398 1305 Size: 3384 3525 184 2007 Fragments arranged by size: 3525 3384 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2696 10 Size: 54 1425 343 864 6414 Fragments arranged by size: 6414 1425 864 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2552 2909 3551 4112 4146 5418 5805 Size: 648 1386 357 642 561 34 1272 387 Cuts at: 5805 7058 7417 7576 518 Size: 1253 359 159 2042 Fragments arranged by size: 2042 1386 1272 1253 648 642 561 387 359 357 159 34 MspA1I CmG'CkG Cuts at: 1606 2155 3136 3594 3669 3695 6389 6516 7193 Size: 549 981 458 75 26 2694 127 677 Cuts at: 7193 8341 8586 1606 Size: 1148 245 2120 Fragments arranged by size: 2694 2120 1148 981 677 549 458 245 127 75 26 NaeI GCC'GGC Cuts at: 4378 4378 Size: 9100 NcoI C'CATG_G Cuts at: 513 1065 3162 513 Size: 552 2097 6451 Fragments arranged by size: 6451 2097 552 NdeI CA'TA_TG Cuts at: 387 5832 387 Size: 5445 3655 NgoMIV G'CCGG_C Cuts at: 4376 4376 Size: 9100 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9100 NotI GC'GGCC_GC Cuts at: 6547 6547 Size: 9100 NsiI A_TGCA'T Cuts at: 5332 5332 Size: 9100 NspI r_CATG'y Cuts at: 1132 1229 6458 8003 1132 Size: 97 5229 1545 2229 Fragments arranged by size: 5229 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2191 1348 Size: 843 8257 PciI A'CATG_T Cuts at: 7999 7999 Size: 9100 PflMI CCAn_nnn'nTGG Cuts at: 1108 2278 2845 3969 1108 Size: 1170 567 1124 6239 Fragments arranged by size: 6239 1170 1124 567 PfoI T'CCnGG_A Cuts at: 6330 6330 Size: 9100 PmeI GTTT'AAAC Cuts at: 6404 6404 Size: 9100 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7169 7201 8682 8714 7169 Size: 32 1481 32 7555 Fragments arranged by size: 7555 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3622 3922 4201 5262 3622 Size: 300 279 1061 7460 Fragments arranged by size: 7460 1061 300 279 PshAI GACnn'nnGTC Cuts at: 1613 2716 3833 4652 1613 Size: 1103 1117 819 6061 Fragments arranged by size: 6061 1117 1103 819 PsiI TTA'TAA Cuts at: 6670 6670 Size: 9100 PspOMI G'GGCC_C Cuts at: 3147 3147 Size: 9100 PspXI vC'TCGA_Gb Cuts at: 5006 5006 Size: 9100 PstI C_TGCA'G Cuts at: 830 2210 4638 5871 6452 830 Size: 1380 2428 1233 581 3478 Fragments arranged by size: 3478 2428 1380 1233 581 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9100 PvuII CAG'CTG Cuts at: 3594 3669 3695 3594 Size: 75 26 8999 Fragments arranged by size: 8999 75 26 SacI G_AGCT'C Cuts at: 5007 5559 5007 Size: 552 8548 SalI G'TCGA_C Cuts at: 1936 6442 1936 Size: 4506 4594 SapI GCTCTTCn'nnn_ Cuts at: 2439 4995 2439 Size: 2556 6544 SbfI CC_TGCA'GG Cuts at: 6452 6452 Size: 9100 ScaI AGT'ACT Cuts at: 5408 7348 9060 5408 Size: 1940 1712 5448 Fragments arranged by size: 5448 1940 1712 SexAI A'CCwGG_T Cuts at: 4829 4829 Size: 9100 SfcI C'TryA_G Cuts at: 826 1045 2206 4634 5867 6448 7583 8264 8455 Size: 219 1161 2428 1233 581 1135 681 191 Cuts at: 8455 826 Size: 1471 Fragments arranged by size: 2428 1471 1233 1161 1135 681 581 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9100 SgrAI Cr'CCGG_yG Cuts at: 1700 3310 3548 1700 Size: 1610 238 7252 Fragments arranged by size: 7252 1610 238 SmaI CCC'GGG Cuts at: 1872 6427 1872 Size: 4555 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2146 2894 5006 5495 7204 8105 Size: 197 926 203 748 2112 489 1709 901 Cuts at: 8105 8367 820 Size: 262 1553 Fragments arranged by size: 2112 1709 1553 926 901 748 489 262 203 197 SnaBI TAC'GTA Cuts at: 493 6891 493 Size: 6398 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 9100 SphI G_CATG'C Cuts at: 1132 6458 1132 Size: 5326 3774 SspI AAT'ATT Cuts at: 5 52 2071 7024 5 Size: 47 2019 4953 2081 Fragments arranged by size: 4953 2081 2019 47 StuI AGG'CCT Cuts at: 1517 3017 4424 1517 Size: 1500 1407 6193 Fragments arranged by size: 6193 1500 1407 StyI C'CwwG_G Cuts at: 513 1065 3162 3444 4488 4743 6539 513 Size: 552 2097 282 1044 255 1796 3074 Fragments arranged by size: 3074 2097 1796 1044 552 282 255 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2138 3175 3279 3280 3619 3918 4309 Size: 1255 558 1037 104 1 339 299 391 Cuts at: 4309 7127 7144 7297 7482 7927 325 Size: 2818 17 153 185 445 1498 Fragments arranged by size: 2818 1498 1255 1037 558 445 391 339 299 185 153 104 17 1 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2610 3060 3633 Size: 274 80 33 51 1443 633 450 573 Cuts at: 3633 4041 4515 4905 5406 6249 6309 7346 9058 Size: 408 474 390 501 843 60 1037 1712 Cuts at: 9058 96 Size: 138 Fragments arranged by size: 1712 1443 1037 843 633 573 501 474 450 408 390 274 138 80 60 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3158 3190 4116 4148 4533 4565 9060 9092 3158 Size: 32 926 32 385 32 4495 32 3166 Fragments arranged by size: 4495 3166 926 385 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3197 1316 Size: 1881 7219 XbaI T'CTAG_A Cuts at: 6436 6436 Size: 9100 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2731 3883 4987 2731 Size: 1152 1104 6844 Fragments arranged by size: 6844 1152 1104 XhoI C'TCGA_G Cuts at: 1943 5006 1943 Size: 3063 6037 XmaI C'CCGG_G Cuts at: 1870 6425 1870 Size: 4555 4545 XmnI GAAnn'nnTTC Cuts at: 2996 7229 2996 Size: 4233 4867 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2187 2836 3882 4105 Size: 53 83 186 936 653 649 1046 223 Cuts at: 4105 6908 276 Size: 2803 2468 Fragments arranged by size: 2803 2468 1046 936 653 649 223 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BsiWI BstZ17I Bsu36I EcoRV FseI FspAI KasI NarI NruI PacI PmlI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI