(Circular) (Six-base) MAPSORT of: pFN21AB0065.seq Check: 6828 from: 1 to: 10828 pFN21AB0065 10828 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4819 7735 4819 Size: 2916 7912 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3432 7832 8008 8638 Size: 53 83 186 936 1896 4400 176 630 Cuts at: 8638 278 Size: 2468 Fragments arranged by size: 4400 2468 1896 936 630 186 176 83 53 AccI GT'mk_AC Cuts at: 1937 5186 8171 1937 Size: 3249 2985 4594 Fragments arranged by size: 4594 3249 2985 Acc65I G'GTAC_C Cuts at: 8149 8149 Size: 10828 AclI AA'CG_TT Cuts at: 1563 8955 9328 1563 Size: 7392 373 3063 Fragments arranged by size: 7392 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2122 2132 2141 2187 3195 3296 3561 3769 Size: 490 10 9 46 1008 101 265 208 Cuts at: 3769 4337 4450 5779 5895 6671 6702 7897 8016 Size: 568 113 1329 116 776 31 1195 119 Cuts at: 8016 8891 10275 10543 1632 Size: 875 1384 268 1917 Fragments arranged by size: 1917 1384 1329 1195 1008 875 776 568 490 268 265 208 119 116 113 101 46 31 10 9 AfeI AGC'GCT Cuts at: 2051 2051 Size: 10828 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 10631 AflIII A'CryG_T Cuts at: 1203 5569 9727 10704 1203 Size: 4366 4158 977 1327 Fragments arranged by size: 4366 4158 1327 977 AgeI A'CCGG_T Cuts at: 9659 9659 Size: 10828 AhdI GACnn_n'nnGTC Cuts at: 6575 9557 6575 Size: 2982 7846 AleI CACnn'nnGTG Cuts at: 1166 4431 4853 1166 Size: 3265 422 7141 Fragments arranged by size: 7141 3265 422 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 7767 7812 1184 Size: 6583 45 4200 Fragments arranged by size: 6583 4200 45 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2487 2519 2487 Size: 32 10796 AlwNI CAG_nnn'CTG Cuts at: 2149 2176 2327 4337 6592 6725 6766 7994 8571 Size: 27 151 2010 2255 133 41 1228 577 Cuts at: 8571 10143 2149 Size: 1572 2834 Fragments arranged by size: 2834 2255 2010 1572 1228 577 151 133 41 27 ApaI G_GGCC'C Cuts at: 2035 7523 2035 Size: 5488 5340 ApaLI G'TGCA_C Cuts at: 8885 10041 8885 Size: 1156 9672 ApoI r'AATT_y Cuts at: 1493 2366 2565 4666 8137 8363 8515 10624 1493 Size: 873 199 2101 3471 226 152 2109 1697 Fragments arranged by size: 3471 2109 2101 1697 873 226 199 152 AseI AT'TA_AT Cuts at: 160 1032 2343 5031 5175 7322 9382 160 Size: 872 1311 2688 144 2147 2060 1606 Fragments arranged by size: 2688 2147 2060 1606 1311 872 144 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 10828 AvaI C'yCGr_G Cuts at: 1870 1943 8153 1870 Size: 73 6210 4545 Fragments arranged by size: 6210 4545 73 AvrII C'CTAG_G Cuts at: 5056 7210 5056 Size: 2154 8674 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 7233 7266 7233 Size: 33 10795 BamHI G'GATC_C Cuts at: 4018 4159 7427 8158 8525 4018 Size: 141 3268 731 367 6321 Fragments arranged by size: 6321 3268 731 367 141 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3011 4204 5123 5845 6959 Size: 325 217 627 1224 1193 919 722 1114 Cuts at: 6959 8149 9604 618 Size: 1190 1455 1842 Fragments arranged by size: 1842 1455 1224 1193 1190 1114 919 722 627 325 217 BanII G_rGCy'C Cuts at: 1391 2035 2132 2424 3414 4940 4964 7523 8147 Size: 644 97 292 990 1526 24 2559 624 Cuts at: 8147 1391 Size: 4072 Fragments arranged by size: 4072 2559 1526 990 644 624 292 97 24 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2100 3432 5236 6121 6659 7018 7303 Size: 971 201 1332 1804 885 538 359 285 Cuts at: 7303 7344 8004 928 Size: 41 660 3752 Fragments arranged by size: 3752 1804 1332 971 885 660 538 359 285 201 41 BbvCI CC'TCA_GC Cuts at: 2682 2682 Size: 10828 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 9019 9053 1903 Size: 34 7082 34 3678 Fragments arranged by size: 7082 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3564 8720 9930 3564 Size: 5156 1210 4462 Fragments arranged by size: 5156 4462 1210 BclI T'GATC_A Cuts at: 1548 2371 2560 2593 2875 5935 1548 Size: 823 189 33 282 3060 6441 Fragments arranged by size: 6441 3060 823 282 189 33 BfrBI ATG'CAT Cuts at: 7472 7724 7472 Size: 252 10576 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5843 9439 136 Size: 107 122 71 631 605 4171 3596 1525 Fragments arranged by size: 4171 3596 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2929 3982 7032 10823 2929 Size: 1053 3050 3791 2934 Fragments arranged by size: 3791 3050 2934 1053 BlpI GC'TnA_GC Cuts at: 4356 5531 8245 4356 Size: 1175 2714 6939 Fragments arranged by size: 6939 2714 1175 Bme1580I G_kGCm'C Cuts at: 1790 2035 4493 5898 6268 7523 8889 10045 1790 Size: 245 2458 1405 370 1255 1366 1156 2573 Fragments arranged by size: 2573 2458 1405 1366 1255 1156 370 245 BmgBI CAC'GTC Cuts at: 1318 7765 1318 Size: 6447 4381 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5844 6754 7544 Size: 416 53 83 399 24 4427 910 790 Cuts at: 7544 7830 8559 9517 442 Size: 286 729 958 1753 Fragments arranged by size: 4427 1753 958 910 790 729 416 399 286 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 10828 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2460 2883 4133 4724 5488 5637 6083 6476 Size: 1221 423 1250 591 764 149 446 393 Cuts at: 6476 7044 7266 7572 7632 7776 9488 1239 Size: 568 222 306 60 144 1712 2579 Fragments arranged by size: 2579 1712 1250 1221 764 591 568 446 423 393 306 222 149 144 60 Bpu10I CC'TnA_GC Cuts at: 2682 4398 6926 7906 2682 Size: 1716 2528 980 5604 Fragments arranged by size: 5604 2528 1716 980 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4316 4554 7078 8953 9818 10116 4316 Size: 238 2524 1875 865 298 5028 Fragments arranged by size: 5028 2524 1875 865 298 238 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2895 3273 7305 9491 882 Size: 617 1396 378 4032 2186 2219 Fragments arranged by size: 4032 2219 2186 1396 617 378 BsaAI yAC'GTr Cuts at: 493 1204 3979 8619 493 Size: 711 2775 4640 2702 Fragments arranged by size: 4640 2775 2702 711 BsaBI GATnn'nnATC Cuts at: 10822 10822 Size: 10828 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3429 7103 7829 8005 Size: 53 83 186 936 1896 3674 726 176 Cuts at: 8005 8635 9017 9652 275 Size: 630 382 635 1451 Fragments arranged by size: 3674 1896 1451 936 726 635 630 382 186 176 83 53 BsaWI w'CCGG_w Cuts at: 2749 6796 9261 9659 9933 10080 10491 2749 Size: 4047 2465 398 274 147 411 3086 Fragments arranged by size: 4047 3086 2465 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 7148 7178 7239 7269 7749 Size: 30 165 30 5745 30 61 30 480 Cuts at: 7749 7779 1178 Size: 30 4227 Fragments arranged by size: 5745 4227 480 165 61 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2414 2499 2880 3049 3400 4635 7169 7569 Size: 1228 85 381 169 351 1235 2534 400 Cuts at: 7569 7773 7854 1186 Size: 204 81 4160 Fragments arranged by size: 4160 2534 1235 1228 400 381 351 204 169 85 81 BseYI C'CCAG_C Cuts at: 2248 2491 6098 6919 6955 10031 10527 2248 Size: 243 3607 821 36 3076 496 2549 Fragments arranged by size: 3607 3076 2549 821 496 243 36 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4917 5753 5908 6750 1163 Size: 573 3181 836 155 842 5241 Fragments arranged by size: 5241 3181 842 836 573 155 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 8278 9039 10067 1540 Size: 275 186 6277 761 1028 2301 Fragments arranged by size: 6277 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2424 3372 3414 4327 4964 8889 8974 10045 2424 Size: 948 42 913 637 3925 85 1071 3207 Fragments arranged by size: 3925 3207 1071 948 913 637 85 42 BsmI GAATG_Cn' Cuts at: 4095 6247 7474 8339 8432 4095 Size: 2152 1227 865 93 6491 Fragments arranged by size: 6491 2152 1227 865 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3786 7755 3786 Size: 3969 6859 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2035 2132 2424 3372 3414 4327 4493 Size: 399 245 97 292 948 42 913 166 Cuts at: 4493 4940 4964 5898 6268 7523 8147 8889 8974 Size: 447 24 934 370 1255 624 742 85 Cuts at: 8974 10045 1391 Size: 1071 2174 Fragments arranged by size: 2174 1255 1071 948 934 913 742 624 447 399 370 292 245 166 97 85 42 24 BspEI T'CCGG_A Cuts at: 10491 10491 Size: 10828 BspHI T'CATG_A Cuts at: 8717 10447 8717 Size: 1730 9098 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2362 4819 4956 6869 7735 8183 844 Size: 1518 2457 137 1913 866 448 3489 Fragments arranged by size: 3489 2457 1913 1518 866 448 137 BsrBI CCG'CTC Cuts at: 1394 1823 8715 1394 Size: 429 6892 3507 Fragments arranged by size: 6892 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2675 2981 3174 4215 5542 7602 8028 Size: 1183 1427 306 193 1041 1327 2060 426 Cuts at: 8028 9323 9497 65 Size: 1295 174 1396 Fragments arranged by size: 2060 1427 1396 1327 1295 1183 1041 426 306 193 174 BsrFI r'CCGG_y Cuts at: 1700 1810 9472 9659 1700 Size: 110 7662 187 2869 Fragments arranged by size: 7662 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2167 96 Size: 2071 8757 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 10828 BssSI C'ACGA_G Cuts at: 5214 8888 9900 5214 Size: 3674 1012 6142 Fragments arranged by size: 6142 3674 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3011 3038 6728 6842 794 Size: 2217 27 3690 114 4780 Fragments arranged by size: 4780 3690 2217 114 27 BstBI TT'CG_AA Cuts at: 5104 9632 5104 Size: 4528 6300 BstEII G'GTnAC_C Cuts at: 1184 4247 6620 1184 Size: 3063 2373 5392 Fragments arranged by size: 5392 3063 2373 BstXI CCAn_nnnn'nTGG Cuts at: 2540 3043 5458 2540 Size: 503 2415 7910 Fragments arranged by size: 7910 2415 503 BstYI r'GATC_y Cuts at: 1972 2795 2929 3982 4018 4159 7032 7427 8158 Size: 823 134 1053 36 141 2873 395 731 Cuts at: 8158 8525 8910 8927 10379 10465 10477 10823 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 2873 1977 1452 1053 823 731 395 385 367 346 141 134 86 36 17 12 Bsu36I CC'TnA_GG Cuts at: 2969 4650 5488 2969 Size: 1681 838 8309 Fragments arranged by size: 8309 1681 838 BtgI C'CryG_G Cuts at: 513 1065 6558 513 Size: 552 5493 4783 Fragments arranged by size: 5493 4783 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4046 7531 487 Size: 683 56 2820 3485 3784 Fragments arranged by size: 3784 3485 2820 683 56 BtsI GCAGTG_nn' Cuts at: 2530 2902 2953 3036 3250 5896 6315 8344 9138 Size: 372 51 83 214 2646 419 2029 794 Cuts at: 9138 9158 2530 Size: 20 4200 Fragments arranged by size: 4200 2646 2029 794 419 372 214 83 51 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 10828 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 10793 DraI TTT'AAA Cuts at: 6439 8132 6439 Size: 1693 9135 DraIII CAC_nnn'GTG Cuts at: 6515 7574 6515 Size: 1059 9769 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 9835 809 Size: 507 8519 1802 Fragments arranged by size: 8519 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4077 5140 6321 Size: 54 1425 50 275 18 2247 1063 1181 Cuts at: 6321 8275 9164 10793 8 Size: 1954 889 1629 43 Fragments arranged by size: 2247 1954 1629 1425 1181 1063 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 8275 1537 Size: 275 6463 4090 Fragments arranged by size: 6463 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2141 3788 4053 4469 4593 5175 5283 5317 Size: 788 1647 265 416 124 582 108 34 Cuts at: 5317 5385 8758 1353 Size: 68 3373 3423 Fragments arranged by size: 3423 3373 1647 788 582 416 265 124 108 68 34 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 9415 9789 9935 387 Size: 1064 138 7826 374 146 1280 Fragments arranged by size: 7826 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2422 3412 4962 2422 Size: 990 1550 8288 Fragments arranged by size: 8288 1550 990 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2122 2132 2141 2187 2460 2883 3195 Size: 393 490 10 9 46 273 423 312 Cuts at: 3195 3296 3561 3769 4133 4337 4450 4724 5488 Size: 101 265 208 364 204 113 274 764 Cuts at: 5488 5637 5779 5895 6083 6476 6671 6702 7044 Size: 149 142 116 188 393 195 31 342 Cuts at: 7044 7266 7572 7632 7776 7897 8016 8891 9488 Size: 222 306 60 144 121 119 875 597 Cuts at: 9488 10275 10543 1239 Size: 787 268 1524 Fragments arranged by size: 1524 875 787 764 597 490 423 393 393 364 342 312 306 274 273 268 265 222 208 204 195 188 149 144 142 121 119 116 113 101 60 46 31 10 9 EcoO109I rG'GnC_Cy Cuts at: 2032 2324 3758 4775 7141 7519 7520 7859 2032 Size: 292 1434 1017 2366 378 1 339 5001 Fragments arranged by size: 5001 2366 1434 1017 378 339 292 1 EcoRI G'AATT_C Cuts at: 8137 8515 8137 Size: 378 10450 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 5809 5809 Size: 10828 FspI TGC'GCA Cuts at: 9334 9334 Size: 10828 HaeII r_GCGC'y Cuts at: 1678 2053 9975 1678 Size: 375 7922 2531 Fragments arranged by size: 7922 2531 375 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2248 2280 2799 2831 3401 Size: 32 135 32 676 32 519 32 570 Cuts at: 3401 3433 3491 3523 4003 4035 4852 4884 5223 Size: 32 58 32 480 32 817 32 339 Cuts at: 5223 5255 5509 5541 5610 5642 6196 6228 6561 Size: 32 254 32 69 32 554 32 333 Cuts at: 6561 6593 7017 7049 9469 9501 9543 9575 1373 Size: 32 424 32 2420 32 42 32 2626 Fragments arranged by size: 2626 2420 817 676 570 554 519 480 424 339 333 254 135 69 58 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4276 7708 8172 8418 669 Size: 988 281 2338 3432 464 246 3079 Fragments arranged by size: 3432 3079 2338 988 464 281 246 HindIII A'AGCT_T Cuts at: 748 5302 748 Size: 4554 6274 HpaI GTT'AAC Cuts at: 4276 7708 8418 4276 Size: 3432 710 6686 Fragments arranged by size: 6686 3432 710 KpnI G_GTAC'C Cuts at: 8153 8153 Size: 10828 MfeI C'AATT_G Cuts at: 4164 5437 8427 4164 Size: 1273 2990 6565 Fragments arranged by size: 6565 2990 1273 MluI A'CGCG_T Cuts at: 10704 10704 Size: 10828 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 1989 3745 6559 7903 9942 10126 1305 Size: 684 1756 2814 1344 2039 184 2007 Fragments arranged by size: 2814 2039 2007 1756 1344 684 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4079 10 Size: 54 1425 343 2247 6759 Fragments arranged by size: 6759 2247 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2343 2538 3843 4431 4769 4853 5121 Size: 648 1177 195 1305 588 338 84 268 Cuts at: 5121 6240 7739 8114 8786 9145 9304 518 Size: 1119 1499 375 672 359 159 2042 Fragments arranged by size: 2042 1499 1305 1177 1119 672 648 588 375 359 338 268 195 159 84 MspA1I CmG'CkG Cuts at: 1606 2176 2252 3569 3774 4928 5591 6003 6419 Size: 570 76 1317 205 1154 663 412 416 Cuts at: 6419 6725 8244 8921 10069 10314 1606 Size: 306 1519 677 1148 245 2120 Fragments arranged by size: 2120 1519 1317 1154 1148 677 663 570 416 412 306 245 205 76 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 10276 NdeI CA'TA_TG Cuts at: 387 4695 6051 387 Size: 4308 1356 5164 Fragments arranged by size: 5164 4308 1356 NheI G'CTAG_C Cuts at: 1052 1052 Size: 10828 NotI GC'GGCC_GC Cuts at: 8275 8275 Size: 10828 NsiI A_TGCA'T Cuts at: 7474 7726 7474 Size: 252 10576 NspI r_CATG'y Cuts at: 1132 1229 2042 5573 7002 8186 9731 1132 Size: 97 813 3531 1429 1184 1545 2229 Fragments arranged by size: 3531 2229 1545 1429 1184 813 97 PasI CC'CwG_GG Cuts at: 1348 2395 1348 Size: 1047 9781 PciI A'CATG_T Cuts at: 5569 9727 5569 Size: 4158 6670 PflMI CCAn_nnn'nTGG Cuts at: 1108 2300 3847 6782 7424 1108 Size: 1192 1547 2935 642 4512 Fragments arranged by size: 4512 2935 1547 1192 642 PfoI T'CCnGG_A Cuts at: 6116 6331 6116 Size: 215 10613 PmeI GTTT'AAAC Cuts at: 8132 8132 Size: 10828 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 8897 8929 10410 10442 8897 Size: 32 1481 32 9283 Fragments arranged by size: 9283 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2324 3758 7859 2324 Size: 1434 4101 5293 Fragments arranged by size: 5293 4101 1434 PshAI GACnn'nnGTC Cuts at: 1613 8004 1613 Size: 6391 4437 PsiI TTA'TAA Cuts at: 4190 5732 8398 4190 Size: 1542 2666 6620 Fragments arranged by size: 6620 2666 1542 PspOMI G'GGCC_C Cuts at: 2031 7519 2031 Size: 5488 5340 PstI C_TGCA'G Cuts at: 830 2127 2634 2722 5278 6163 7191 8180 830 Size: 1297 507 88 2556 885 1028 989 3478 Fragments arranged by size: 3478 2556 1297 1028 989 885 507 88 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 10828 PvuII CAG'CTG Cuts at: 2176 2252 3569 4928 5591 6003 6419 6725 2176 Size: 76 1317 1359 663 412 416 306 6279 Fragments arranged by size: 6279 1359 1317 663 416 412 306 76 SacI G_AGCT'C Cuts at: 2424 3414 4964 2424 Size: 990 1550 8288 Fragments arranged by size: 8288 1550 990 SalI G'TCGA_C Cuts at: 1936 8170 1936 Size: 6234 4594 SapI GCTCTTCn'nnn_ Cuts at: 3788 3788 Size: 10828 SbfI CC_TGCA'GG Cuts at: 8180 8180 Size: 10828 ScaI AGT'ACT Cuts at: 4683 9076 10788 4683 Size: 4393 1712 4723 Fragments arranged by size: 4723 4393 1712 SexAI A'CCwGG_T Cuts at: 2184 2326 2184 Size: 142 10686 SfcI C'TryA_G Cuts at: 826 1045 2123 2630 2718 3873 4305 5274 5706 Size: 219 1078 507 88 1155 432 969 432 Cuts at: 5706 6159 6597 7129 7187 8176 9311 9992 10183 Size: 453 438 532 58 989 1135 681 191 Cuts at: 10183 826 Size: 1471 Fragments arranged by size: 1471 1155 1135 1078 989 969 681 532 507 453 438 432 432 219 191 88 58 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 10828 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 10828 SmaI CCC'GGG Cuts at: 1872 8155 1872 Size: 6283 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4295 4569 7057 8932 9833 10095 Size: 197 926 2352 274 2488 1875 901 262 Cuts at: 10095 820 Size: 1553 Fragments arranged by size: 2488 2352 1875 1553 926 901 274 262 197 SnaBI TAC'GTA Cuts at: 493 8619 493 Size: 8126 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 10828 SphI G_CATG'C Cuts at: 1132 2042 8186 1132 Size: 910 6144 3774 Fragments arranged by size: 6144 3774 910 SspI AAT'ATT Cuts at: 5 52 2194 2218 2499 8752 5 Size: 47 2142 24 281 6253 2081 Fragments arranged by size: 6253 2142 2081 281 47 24 StuI AGG'CCT Cuts at: 1517 2445 7209 1517 Size: 928 4764 5136 Fragments arranged by size: 5136 4764 928 StyI C'CwwG_G Cuts at: 513 1065 2446 3157 5056 5741 6465 7210 8267 Size: 552 1381 711 1899 685 724 745 1057 Cuts at: 8267 513 Size: 3074 Fragments arranged by size: 3074 1899 1381 1057 745 724 711 685 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3049 3928 3935 4266 5863 6126 6947 Size: 1255 1469 879 7 331 1597 263 821 Cuts at: 6947 7370 7620 7712 8078 8855 8872 9025 9210 Size: 423 250 92 366 777 17 153 185 Cuts at: 9210 9655 325 Size: 445 1498 Fragments arranged by size: 1597 1498 1469 1255 879 821 777 445 423 366 331 263 250 185 153 92 17 7 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2167 4052 4681 Size: 274 80 33 51 1443 190 1885 629 Cuts at: 4681 5787 7433 9074 10786 96 Size: 1106 1646 1641 1712 138 Fragments arranged by size: 1885 1712 1646 1641 1443 1106 629 274 190 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3351 3383 7148 7180 7239 7271 7749 7781 10788 Size: 32 3765 32 59 32 478 32 3007 Cuts at: 10788 10820 3351 Size: 32 3359 Fragments arranged by size: 3765 3359 3007 478 59 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 7265 1316 Size: 5949 4879 XbaI T'CTAG_A Cuts at: 6199 8050 8164 6199 Size: 1851 114 8863 Fragments arranged by size: 8863 1851 114 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 10828 XmaI C'CCGG_G Cuts at: 1870 8153 1870 Size: 6283 4545 XmnI GAAnn'nnTTC Cuts at: 3782 4062 5233 5685 6372 7341 8957 3782 Size: 280 1171 452 687 969 1616 5653 Fragments arranged by size: 5653 1616 1171 969 687 452 280 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3430 7830 8006 8636 Size: 53 83 186 936 1896 4400 176 630 Cuts at: 8636 276 Size: 2468 Fragments arranged by size: 4400 2468 1896 936 630 186 176 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI BbeI BplI BsiWI BstZ17I EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI XcmI