(Circular) (Six-base) MAPSORT of: pFN21AB0063.seq Check: 678 from: 1 to: 11308 pFN21AB0063 11308 bp With 182 enzymes: SgfI * January 31, 2011 17:48 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2021 2999 3047 8324 2021 Size: 978 48 5277 5005 Fragments arranged by size: 5277 5005 978 48 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4031 9118 278 Size: 53 83 186 936 2495 5087 2468 Fragments arranged by size: 5087 2495 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3168 6522 8651 1937 Size: 1231 3354 2129 4594 Fragments arranged by size: 4594 3354 2129 1231 Acc65I G'GTAC_C Cuts at: 8629 8629 Size: 11308 AclI AA'CG_TT Cuts at: 1563 9435 9808 1563 Size: 7872 373 3063 Fragments arranged by size: 7872 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2229 2488 2532 4170 4409 4613 4961 5713 Size: 597 259 44 1638 239 204 348 752 Cuts at: 5713 8327 8560 9371 10755 11023 1632 Size: 2614 233 811 1384 268 1917 Fragments arranged by size: 2614 1917 1638 1384 811 752 597 348 268 259 239 233 204 44 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 11111 AflIII A'CryG_T Cuts at: 1203 2789 4641 4705 5200 10207 11184 1203 Size: 1586 1852 64 495 5007 977 1327 Fragments arranged by size: 5007 1852 1586 1327 977 495 64 AgeI A'CCGG_T Cuts at: 7748 10139 7748 Size: 2391 8917 AhdI GACnn_n'nnGTC Cuts at: 3551 3815 4027 10037 3551 Size: 264 212 6010 4822 Fragments arranged by size: 6010 4822 264 212 AleI CACnn'nnGTG Cuts at: 1166 3153 3987 1166 Size: 1987 834 8487 Fragments arranged by size: 8487 1987 834 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2442 1184 Size: 1258 10050 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3989 4021 4562 4594 8523 8555 3989 Size: 32 541 32 3929 32 6742 Fragments arranged by size: 6742 3929 541 32 32 32 AlwNI CAG_nnn'CTG Cuts at: 2354 2676 3516 3702 3795 8448 9051 10623 2354 Size: 322 840 186 93 4653 603 1572 3039 Fragments arranged by size: 4653 3039 1572 840 603 322 186 93 ApaI G_GGCC'C Cuts at: 4382 7623 7887 4382 Size: 3241 264 7803 Fragments arranged by size: 7803 3241 264 ApaLI G'TGCA_C Cuts at: 9365 10521 9365 Size: 1156 10152 ApoI r'AATT_y Cuts at: 1493 4160 5036 6725 7174 8162 8617 8843 8995 Size: 2667 876 1689 449 988 455 226 152 Cuts at: 8995 11104 1493 Size: 2109 1697 Fragments arranged by size: 2667 2109 1697 1689 988 876 455 449 226 152 AseI AT'TA_AT Cuts at: 160 1032 6624 9862 160 Size: 872 5592 3238 1606 Fragments arranged by size: 5592 3238 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 11308 AvaI C'yCGr_G Cuts at: 1870 1943 3820 4374 6217 6224 6356 7574 7734 Size: 73 1877 554 1843 7 132 1218 160 Cuts at: 7734 8633 1870 Size: 899 4545 Fragments arranged by size: 4545 1877 1843 1218 899 554 160 132 73 7 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 7919 7952 7919 Size: 33 11275 BamHI G'GATC_C Cuts at: 2186 4201 8638 9005 2186 Size: 2015 4437 367 4489 Fragments arranged by size: 4489 4437 2015 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2236 2987 7198 8629 10084 Size: 325 217 627 449 751 4211 1431 1455 Cuts at: 10084 618 Size: 1842 Fragments arranged by size: 4211 1842 1455 1431 751 627 449 325 217 BanII G_rGCy'C Cuts at: 1391 2253 2568 2943 3222 4382 5679 7139 7623 Size: 862 315 375 279 1160 1297 1460 484 Cuts at: 7623 7634 7844 7887 8220 8627 1391 Size: 11 210 43 333 407 4072 Fragments arranged by size: 4072 1460 1297 1160 862 484 407 375 333 315 279 210 43 11 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2293 2397 2934 3027 3492 3864 4920 Size: 971 394 104 537 93 465 372 1056 Cuts at: 4920 5027 6934 8032 928 Size: 107 1907 1098 4204 Fragments arranged by size: 4204 1907 1098 1056 971 537 465 394 372 107 104 93 BbvCI CC'TCA_GC Cuts at: 4812 5478 6297 8025 4812 Size: 666 819 1728 8095 Fragments arranged by size: 8095 1728 819 666 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2583 2617 2701 2735 2807 2841 9499 Size: 34 646 34 84 34 72 34 6658 Cuts at: 9499 9533 1903 Size: 34 3678 Fragments arranged by size: 6658 3678 646 84 72 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 9200 10410 9200 Size: 1210 10098 BclI T'GATC_A Cuts at: 1548 4464 1548 Size: 2916 8392 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2820 3750 Size: 107 122 71 631 605 337 811 930 Cuts at: 3750 4127 7413 9919 136 Size: 377 3286 2506 1525 Fragments arranged by size: 3286 2506 1525 930 811 631 605 377 337 122 107 71 BglII A'GATC_T Cuts at: 11303 11303 Size: 11308 BlpI GC'TnA_GC Cuts at: 2372 8725 2372 Size: 6353 4955 Bme1580I G_kGCm'C Cuts at: 1790 2769 4382 7201 7242 7623 7887 9369 10525 Size: 979 1613 2819 41 381 264 1482 1156 Cuts at: 10525 1790 Size: 2573 Fragments arranged by size: 2819 2573 1613 1482 1156 979 381 264 41 BmgBI CAC'GTC Cuts at: 1318 4528 4706 1318 Size: 3210 178 7920 Fragments arranged by size: 7920 3210 178 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2185 2756 3303 Size: 416 53 83 399 24 768 571 547 Cuts at: 3303 3690 3783 9039 9997 442 Size: 387 93 5256 958 1753 Fragments arranged by size: 5256 1753 958 768 571 547 416 399 387 93 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 11308 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2228 2402 2411 2432 3222 4070 4112 4818 Size: 989 174 9 21 790 848 42 706 Cuts at: 4818 5550 6461 6776 7213 9968 1239 Size: 732 911 315 437 2755 2579 Fragments arranged by size: 2755 2579 989 911 848 790 732 706 437 315 174 42 21 9 Bpu10I CC'TnA_GC Cuts at: 4812 5229 5478 6297 7838 8025 4812 Size: 417 249 819 1541 187 8095 Fragments arranged by size: 8095 1541 819 417 249 187 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2063 3206 5390 6856 9433 10298 10596 2063 Size: 1143 2184 1466 2577 865 298 2775 Fragments arranged by size: 2775 2577 2184 1466 1143 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4292 4530 4658 4991 6395 6805 7046 Size: 617 2793 238 128 333 1404 410 241 Cuts at: 7046 8557 9971 882 Size: 1511 1414 2219 Fragments arranged by size: 2793 2219 1511 1414 1404 617 410 333 241 238 128 BsaAI yAC'GTr Cuts at: 493 1204 4134 9099 493 Size: 711 2930 4965 2702 Fragments arranged by size: 4965 2930 2702 711 BsaBI GATnn'nnATC Cuts at: 11302 11302 Size: 11308 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4028 9115 9497 10132 Size: 53 83 186 936 2495 5087 382 635 Cuts at: 10132 275 Size: 1451 Fragments arranged by size: 5087 2495 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4242 6549 6865 7224 7748 9741 10139 10413 10560 Size: 2307 316 359 524 1993 398 274 147 Cuts at: 10560 10971 4242 Size: 411 4579 Fragments arranged by size: 4579 2307 1993 524 411 398 359 316 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2882 2912 3068 3098 3209 Size: 30 165 30 1479 30 156 30 111 Cuts at: 3209 3239 4562 4592 5502 5532 1178 Size: 30 1323 30 910 30 6954 Fragments arranged by size: 6954 1479 1323 910 165 156 111 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2399 2792 3089 3867 4303 5780 7906 1186 Size: 1213 393 297 778 436 1477 2126 4588 Fragments arranged by size: 4588 2126 1477 1213 778 436 393 297 BseYI C'CCAG_C Cuts at: 2252 2669 4421 4654 5262 5535 5808 6052 6081 Size: 417 1752 233 608 273 273 244 29 Cuts at: 6081 6542 10511 11007 2252 Size: 461 3969 496 2553 Fragments arranged by size: 3969 2553 1752 608 496 461 417 273 273 244 233 29 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3606 4128 4337 5111 5348 5621 5966 Size: 573 1870 522 209 774 237 273 345 Cuts at: 5966 6167 1163 Size: 201 6304 Fragments arranged by size: 6304 1870 774 573 522 345 273 237 209 201 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2494 2968 3439 8758 9519 10547 Size: 275 186 493 474 471 5319 761 1028 Cuts at: 10547 1540 Size: 2301 Fragments arranged by size: 5319 2301 1028 761 493 474 471 275 186 BsiHKAI G_wGCw'C Cuts at: 2274 2784 2943 3222 5313 5472 6132 7634 8220 Size: 510 159 279 2091 159 660 1502 586 Cuts at: 8220 8416 9369 9454 10525 2274 Size: 196 953 85 1071 3057 Fragments arranged by size: 3057 2091 1502 1071 953 660 586 510 279 196 159 159 85 BsmI GAATG_Cn' Cuts at: 4107 6670 8819 8912 4107 Size: 2563 2149 93 6503 Fragments arranged by size: 6503 2563 2149 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3823 3908 8080 3823 Size: 85 4172 7051 Fragments arranged by size: 7051 4172 85 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2253 2274 2568 2769 2784 2943 3222 Size: 399 463 21 294 201 15 159 279 Cuts at: 3222 4382 5313 5472 5679 6132 7139 7201 7242 Size: 1160 931 159 207 453 1007 62 41 Cuts at: 7242 7623 7634 7844 7887 8220 8416 8627 9369 Size: 381 11 210 43 333 196 211 742 Cuts at: 9369 9454 10525 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1160 1071 1007 931 742 463 453 399 381 333 294 279 211 210 207 201 196 159 159 85 62 43 41 21 15 11 BspEI T'CCGG_A Cuts at: 4242 6549 7224 10971 4242 Size: 2307 675 3747 4579 Fragments arranged by size: 4579 3747 2307 675 BspHI T'CATG_A Cuts at: 9197 10927 9197 Size: 1730 9578 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2021 2999 3047 4150 5676 7764 8238 8324 Size: 1177 978 48 1103 1526 2088 474 86 Cuts at: 8324 8663 844 Size: 339 3489 Fragments arranged by size: 3489 2088 1526 1177 1103 978 474 339 86 48 BsrBI CCG'CTC Cuts at: 1394 1823 9195 1394 Size: 429 7372 3507 Fragments arranged by size: 7372 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2426 2686 4212 7470 7971 9803 9977 Size: 1183 1178 260 1526 3258 501 1832 174 Cuts at: 9977 65 Size: 1396 Fragments arranged by size: 3258 1832 1526 1396 1183 1178 501 260 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2598 3066 3107 3572 4308 7748 9952 Size: 110 788 468 41 465 736 3440 2204 Cuts at: 9952 10139 1700 Size: 187 2869 Fragments arranged by size: 3440 2869 2204 788 736 468 465 187 110 41 BsrGI T'GTAC_A Cuts at: 96 3969 96 Size: 3873 7435 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 11308 BssSI C'ACGA_G Cuts at: 3746 3983 9368 10380 3746 Size: 237 5385 1012 4674 Fragments arranged by size: 5385 4674 1012 237 BstAPI GCAn_nnn'nTGC Cuts at: 794 3675 8135 794 Size: 2881 4460 3967 Fragments arranged by size: 4460 3967 2881 BstBI TT'CG_AA Cuts at: 2721 10112 2721 Size: 7391 3917 BstEII G'GTnAC_C Cuts at: 1184 4396 7751 1184 Size: 3212 3355 4741 Fragments arranged by size: 4741 3355 3212 BstXI CCAn_nnnn'nTGG Cuts at: 3989 4411 4957 5344 7399 3989 Size: 422 546 387 2055 7898 Fragments arranged by size: 7898 2055 546 422 387 BstYI r'GATC_y Cuts at: 1972 2186 2203 4201 6040 6393 7866 8363 8638 Size: 214 17 1998 1839 353 1473 497 275 Cuts at: 8638 9005 9390 9407 10859 10945 10957 11303 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1998 1977 1839 1473 1452 497 385 367 353 346 275 214 86 17 17 12 Bsu36I CC'TnA_GG Cuts at: 2411 4286 5951 6200 7889 2411 Size: 1875 1665 249 1689 5830 Fragments arranged by size: 5830 1875 1689 1665 249 BtgI C'CryG_G Cuts at: 513 1065 2004 2875 3247 3613 5710 5934 7131 Size: 552 939 871 372 366 2097 224 1197 Cuts at: 7131 7263 7584 8239 8343 513 Size: 132 321 655 104 3478 Fragments arranged by size: 3478 2097 1197 939 871 655 552 372 366 321 224 132 104 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2294 2847 7327 487 Size: 683 56 1068 553 4480 4468 Fragments arranged by size: 4480 4468 1068 683 553 56 BtsI GCAGTG_nn' Cuts at: 2621 2779 2941 3089 3247 3275 3406 3647 4455 Size: 158 162 148 158 28 131 241 808 Cuts at: 4455 7641 8824 9618 9638 2621 Size: 3186 1183 794 20 4291 Fragments arranged by size: 4291 3186 1183 808 794 241 162 158 158 148 131 28 20 ClaI AT'CG_AT Cuts at: 1554 4494 4551 1554 Size: 2940 57 8311 Fragments arranged by size: 8311 2940 57 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 11273 DraI TTT'AAA Cuts at: 8612 8612 Size: 11308 DraIII CAC_nnn'GTG Cuts at: 4360 4360 Size: 11308 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7490 10315 809 Size: 507 6174 2825 1802 Fragments arranged by size: 6174 2825 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3157 3244 3250 Size: 54 1425 50 275 18 1327 87 6 Cuts at: 3250 3436 3616 3709 3730 3772 4128 5031 5523 Size: 186 180 93 21 42 356 903 492 Cuts at: 5523 7452 8755 9644 11273 8 Size: 1929 1303 889 1629 43 Fragments arranged by size: 1929 1629 1425 1327 1303 903 889 492 356 275 186 180 93 87 54 50 43 42 21 18 6 EagI C'GGCC_G Cuts at: 1537 1812 3436 8755 1537 Size: 275 1624 5319 4090 Fragments arranged by size: 5319 4090 1624 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2127 3122 3906 4097 4385 5889 7622 9238 Size: 774 995 784 191 288 1504 1733 1616 Cuts at: 9238 1353 Size: 3423 Fragments arranged by size: 3423 1733 1616 1504 995 784 774 288 191 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 9895 10269 10415 387 Size: 1064 138 8306 374 146 1280 Fragments arranged by size: 8306 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2941 3220 7632 8218 2941 Size: 279 4412 586 6031 Fragments arranged by size: 6031 4412 586 279 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2228 2229 2402 2411 2432 2488 2532 Size: 393 596 1 173 9 21 56 44 Cuts at: 2532 3222 4070 4112 4170 4409 4613 4818 4961 Size: 690 848 42 58 239 204 205 143 Cuts at: 4961 5550 5713 6461 6776 7213 8327 8560 9371 Size: 589 163 748 315 437 1114 233 811 Cuts at: 9371 9968 10755 11023 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1114 848 811 787 748 690 597 596 589 437 393 315 268 239 233 205 204 173 163 143 58 56 44 42 21 9 1 EcoNI CCTnn'n_nnAGG Cuts at: 2457 7377 7453 2457 Size: 4920 76 6312 Fragments arranged by size: 6312 4920 76 EcoO109I rG'GnC_Cy Cuts at: 2074 2478 2549 3529 4378 4379 4972 5574 6586 Size: 404 71 980 849 1 593 602 1012 Cuts at: 6586 7619 7620 7884 2074 Size: 1033 1 264 5498 Fragments arranged by size: 5498 1033 1012 980 849 602 593 404 264 71 1 1 EcoRI G'AATT_C Cuts at: 6725 8162 8617 8995 6725 Size: 1437 455 378 9038 Fragments arranged by size: 9038 1437 455 378 EcoRV GAT'ATC Cuts at: 4347 4347 Size: 11308 FspI TGC'GCA Cuts at: 9814 9814 Size: 11308 HaeII r_GCGC'y Cuts at: 1678 4122 8033 10455 1678 Size: 2444 3911 2422 2531 Fragments arranged by size: 3911 2531 2444 2422 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2195 2227 2697 2729 3537 Size: 32 135 32 623 32 470 32 808 Cuts at: 3537 3569 3801 3833 4013 4045 6237 6269 6352 Size: 32 232 32 180 32 2192 32 83 Cuts at: 6352 6384 7394 7426 7685 7717 7802 7834 7970 Size: 32 1010 32 259 32 85 32 136 Cuts at: 7970 8002 8039 8071 9949 9981 10023 10055 1373 Size: 32 37 32 1878 32 42 32 2626 Fragments arranged by size: 2626 2192 1878 1010 808 623 470 259 232 180 136 135 85 83 42 37 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3922 6157 6874 8461 8506 8652 Size: 988 281 1984 2235 717 1587 45 146 Cuts at: 8652 8898 669 Size: 246 3079 Fragments arranged by size: 3079 2235 1984 1587 988 717 281 246 146 45 HindIII A'AGCT_T Cuts at: 748 2260 748 Size: 1512 9796 HpaI GTT'AAC Cuts at: 8506 8898 8506 Size: 392 10916 KpnI G_GTAC'C Cuts at: 8633 8633 Size: 11308 MfeI C'AATT_G Cuts at: 2863 7923 8907 2863 Size: 5060 984 5264 Fragments arranged by size: 5264 5060 984 MluI A'CGCG_T Cuts at: 11184 11184 Size: 11308 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4938 6642 7683 10422 10606 1305 Size: 3633 1704 1041 2739 184 2007 Fragments arranged by size: 3633 2739 2007 1704 1041 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3246 3711 3774 4130 5033 Size: 54 1425 343 1414 465 63 356 903 Cuts at: 5033 7454 10 Size: 2421 3864 Fragments arranged by size: 3864 2421 1425 1414 903 465 356 343 63 54 MslI CAynn'nnrTG Cuts at: 518 1166 3153 3987 5324 5597 6143 7346 9266 Size: 648 1987 834 1337 273 546 1203 1920 Cuts at: 9266 9625 9784 518 Size: 359 159 2042 Fragments arranged by size: 2042 1987 1920 1337 1203 834 648 546 359 273 159 MspA1I CmG'CkG Cuts at: 1606 2023 2659 2976 3162 3255 3348 3441 3720 Size: 417 636 317 186 93 93 93 279 Cuts at: 3720 3828 3852 5294 5712 6052 6113 6929 7191 Size: 108 24 1442 418 340 61 816 262 Cuts at: 7191 8724 9401 10549 10794 1606 Size: 1533 677 1148 245 2120 Fragments arranged by size: 2120 1533 1442 1148 816 677 636 418 417 340 317 279 262 245 186 108 93 93 93 61 24 NcoI C'CATG_G Cuts at: 513 1065 2004 3247 3613 5934 7131 7263 8239 Size: 552 939 1243 366 2321 1197 132 976 Cuts at: 8239 8343 513 Size: 104 3478 Fragments arranged by size: 3478 2321 1243 1197 976 939 552 366 132 104 NdeI CA'TA_TG Cuts at: 387 8131 387 Size: 7744 3564 NheI G'CTAG_C Cuts at: 1052 1052 Size: 11308 NotI GC'GGCC_GC Cuts at: 8755 8755 Size: 11308 NspI r_CATG'y Cuts at: 1132 1229 2793 3050 3457 4645 5204 8666 10211 Size: 97 1564 257 407 1188 559 3462 1545 Cuts at: 10211 1132 Size: 2229 Fragments arranged by size: 3462 2229 1564 1545 1188 559 407 257 97 PasI CC'CwG_GG Cuts at: 1348 3061 3510 8203 1348 Size: 1713 449 4693 4453 Fragments arranged by size: 4693 4453 1713 449 PciI A'CATG_T Cuts at: 2789 4641 5200 10207 2789 Size: 1852 559 5007 3890 Fragments arranged by size: 5007 3890 1852 559 PflMI CCAn_nnn'nTGG Cuts at: 1108 2146 5083 1108 Size: 1038 2937 7333 Fragments arranged by size: 7333 2937 1038 PfoI T'CCnGG_A Cuts at: 4041 4089 5874 6374 6647 6920 7541 4041 Size: 48 1785 500 273 273 621 7808 Fragments arranged by size: 7808 1785 621 500 273 273 48 PmeI GTTT'AAAC Cuts at: 8612 8612 Size: 11308 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4562 4594 7273 7305 9377 9409 10890 10922 4562 Size: 32 2679 32 2072 32 1481 32 4948 Fragments arranged by size: 4948 2679 2072 1481 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2074 4972 2074 Size: 2898 8410 PshAI GACnn'nnGTC Cuts at: 1613 5656 1613 Size: 4043 7265 PsiI TTA'TAA Cuts at: 8878 8878 Size: 11308 PspOMI G'GGCC_C Cuts at: 4378 7619 7883 4378 Size: 3241 264 7803 Fragments arranged by size: 7803 3241 264 PspXI vC'TCGA_Gb Cuts at: 6224 7574 6224 Size: 1350 9958 PstI C_TGCA'G Cuts at: 830 2431 2654 2747 2774 2936 3122 3242 3308 Size: 1601 223 93 27 162 186 120 66 Cuts at: 3308 3401 3521 4996 8660 830 Size: 93 120 1475 3664 3478 Fragments arranged by size: 3664 3478 1601 1475 223 186 162 120 120 93 93 66 27 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 11308 PvuII CAG'CTG Cuts at: 2023 2659 5294 6052 6113 6929 7191 2023 Size: 636 2635 758 61 816 262 6140 Fragments arranged by size: 6140 2635 816 758 636 262 61 SacI G_AGCT'C Cuts at: 2943 3222 7634 8220 2943 Size: 279 4412 586 6031 Fragments arranged by size: 6031 4412 586 279 SacII CC_GC'GG Cuts at: 5713 5713 Size: 11308 SalI G'TCGA_C Cuts at: 1936 8650 1936 Size: 6714 4594 SapI GCTCTTCn'nnn_ Cuts at: 4097 7622 4097 Size: 3525 7783 SbfI CC_TGCA'GG Cuts at: 8660 8660 Size: 11308 ScaI AGT'ACT Cuts at: 4067 4577 5585 6288 6508 9556 11268 4067 Size: 510 1008 703 220 3048 1712 4107 Fragments arranged by size: 4107 3048 1712 1008 703 510 220 SexAI A'CCwGG_T Cuts at: 2397 4469 5886 2397 Size: 2072 1417 7819 Fragments arranged by size: 7819 2072 1417 SfcI C'TryA_G Cuts at: 826 1045 2427 2485 2650 2743 2770 2932 3118 Size: 219 1382 58 165 93 27 162 186 Cuts at: 3118 3238 3304 3397 3517 3847 4992 5455 6021 Size: 120 66 93 120 330 1145 463 566 Cuts at: 6021 6274 7116 7822 8656 9791 10472 10663 826 Size: 253 842 706 834 1135 681 191 1471 Fragments arranged by size: 1471 1382 1145 1135 842 834 706 681 566 463 330 253 219 191 186 165 162 120 120 93 93 66 58 27 SfiI GGCCn_nnn'nGGCC Cuts at: 7413 7413 Size: 11308 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 11308 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 11308 SmaI CCC'GGG Cuts at: 1872 4376 6219 7736 8635 1872 Size: 2504 1843 1517 899 4545 Fragments arranged by size: 4545 2504 1843 1517 899 SmlI C'TyrA_G Cuts at: 820 1017 1943 2078 3221 5405 6224 6835 7574 Size: 197 926 135 1143 2184 819 611 739 Cuts at: 7574 9412 10313 10575 820 Size: 1838 901 262 1553 Fragments arranged by size: 2184 1838 1553 1143 926 901 819 739 611 262 197 135 SnaBI TAC'GTA Cuts at: 493 9099 493 Size: 8606 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 11308 SphI G_CATG'C Cuts at: 1132 8666 1132 Size: 7534 3774 SspI AAT'ATT Cuts at: 5 52 9232 5 Size: 47 9180 2081 Fragments arranged by size: 9180 2081 47 StuI AGG'CCT Cuts at: 1517 3762 4155 4680 5130 5303 5483 5949 6302 Size: 2245 393 525 450 173 180 466 353 Cuts at: 6302 6768 1517 Size: 466 6057 Fragments arranged by size: 6057 2245 525 466 466 450 393 353 180 173 StyI C'CwwG_G Cuts at: 513 1065 2004 3247 3613 5934 6814 7131 7263 Size: 552 939 1243 366 2321 880 317 132 Cuts at: 7263 7449 7947 8239 8343 8747 513 Size: 186 498 292 104 404 3074 Fragments arranged by size: 3074 2321 1243 939 880 552 498 404 366 317 292 186 132 104 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3074 3995 5071 5813 6827 7216 7583 Size: 1255 1494 921 1076 742 1014 389 367 Cuts at: 7583 7865 9335 9352 9505 9690 10135 325 Size: 282 1470 17 153 185 445 1498 Fragments arranged by size: 1498 1494 1470 1255 1076 1014 921 742 445 389 367 282 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2423 3969 4065 Size: 274 80 33 51 1443 446 1546 96 Cuts at: 4065 4575 5148 5583 6286 6506 9554 11266 96 Size: 510 573 435 703 220 3048 1712 138 Fragments arranged by size: 3048 1712 1546 1443 703 573 510 446 435 274 220 138 96 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2871 2882 2903 2914 3068 3100 3209 3241 3533 Size: 11 21 11 154 32 109 32 292 Cuts at: 3533 3565 3719 3751 4181 4213 5502 5534 8061 Size: 32 154 32 430 32 1289 32 2527 Cuts at: 8061 8093 8320 8352 11268 11300 2871 Size: 32 227 32 2916 32 2879 Fragments arranged by size: 2916 2879 2527 1289 430 292 227 154 154 109 32 32 32 32 32 32 32 32 32 21 11 11 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 11308 XbaI T'CTAG_A Cuts at: 4207 8644 4207 Size: 4437 6871 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2464 4408 6436 7210 7270 7477 7877 2464 Size: 1944 2028 774 60 207 400 5895 Fragments arranged by size: 5895 2028 1944 774 400 207 60 XhoI C'TCGA_G Cuts at: 1943 6224 7574 1943 Size: 4281 1350 5677 Fragments arranged by size: 5677 4281 1350 XmaI C'CCGG_G Cuts at: 1870 4374 6217 7734 8633 1870 Size: 2504 1843 1517 899 4545 Fragments arranged by size: 4545 2504 1843 1517 899 XmnI GAAnn'nnTTC Cuts at: 2262 9437 2262 Size: 7175 4133 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4029 9116 276 Size: 53 83 186 936 2495 5087 2468 Fragments arranged by size: 5087 2495 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BbeI BfrBI BplI BsiWI BstZ17I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PsrI RsrII SanDI SfoI SrfI SwaI