Restriction map for
pFN21AB0020
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# NO CUT ENZYMES ( 51 enzymes) #
##################################
AhdI AscI AseI AsnI AspEI
AviII BbvCI BglII BsaBI BsiWI
Bsp1407I BspDI BspHI BspLU11I BsrGI
BssHII Bst1107I BstZ17I ClaI CpoI
DraIII Eam1105I EcoRI EcoT22I FseI
FspI MamI MluI NcoI NheI
NotI NruI NsiI NspI PacI
PaeR7I PmeI PshBI PvuI RcaI
RsrII SalI ScaI SexAI SfiI
SphI SspBI SspI SwaI XcmI
XhoI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AatII 4719 GACGTC GACGT^C
Acc65I 3607 GGTACC G^GTACC
AccI 1003 GTAGAC GT^[AC][GT]AC
AccII 123 CGCG CG^CG
AclI 4326 AACGTT AA^CGTT
AflII 4485 CTTAAG C^TTAAG
AgeI 3175 ACCGGT A^CCGGT
Aor51HI 1918 AGCGCT AGC^GCT
ApaI 4426 GGGCCC GGGCC^C
Asp700I 439 GAACAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3607 GGTACC G^GTACC
AvrII 4197 CCTAGG C^CTAGG
BamHI 2180 GGATCC G^GATCC
BclI 4959 TGATCA T^GATCA
BfrI 4485 CTTAAG C^TTAAG
BlnI 4197 CCTAGG C^CTAGG
BsmI 790 GAATGC GAATGC(1/-1)
BsrBI 4394 CCGCTC CCGCTC(-3/-3)
BsrDI 2116 GCAATG GCAATG(2/0)
BssSI 1096 CACGAG CACGAG(-5/-1)
BstBI 3841 TTCGAA TT^CGAA
BstEII 235 GGTGACC G^GT[ACGT]ACC
BstPI 235 GGTGACC G^GT[ACGT]ACC
BstUI 123 CGCG CG^CG
BstYI 2180 GGATCC [GA]^GATC[CT]
DrdI 2518 GACTCTGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1918 AGCGCT AGC^GCT
EcoO65I 235 GGTGACC G^GT[ACGT]ACC
EcoRV 2739 GATATC GAT^ATC
FbaI 4959 TGATCA T^GATCA
KpnI 3607 GGTACC GGTAC^C
KspI 122 CCGCGG CCGC^GG
MfeI 4586 CAATTG C^AATTG
MflI 2180 GGATCC [GA]^GATC[CT]
MunI 4586 CAATTG C^AATTG
MvnI 123 CGCG CG^CG
NdeI 2668 CATATG CA^TATG
NspV 3841 TTCGAA TT^CGAA
PinAI 3175 ACCGGT A^CCGGT
PshAI 1441 GACCTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 4326 AACGTT AA^CGTT
SacII 122 CCGCGG CCGC^GG
SbfI 4336 CCTGCAGG CCTGCA^GG
SfuI 3841 TTCGAA TT^CGAA
SgrAI 3174 CACCGGTG C[GA]^CCGG[CT]G
SmaI 2879 CCCGGG CCC^GGG
SnaBI 4688 TACGTA TAC^GTA
Sse8387I 4336 CCTGCAGG CCTGCA^GG
XbaI 3726 TCTAGA T^CTAGA
XhoII 2180 GGATCC [GA]^GATC[CT]
XmaCI 2879 CCCGGG C^CCGGG
XmaI 2879 CCCGGG C^CCGGG
XmnI 439 GAACAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccIII 1414 TCCGGA T^CCGGA
1662 TCCGGA
AflIII 1405 ACGTGT A^C[GA][CT]GT
1729 ACGTGT
Alw44I 4343 GTGCAC G^TGCAC
5254 GTGCAC
ApaLI 4343 GTGCAC G^TGCAC
5254 GTGCAC
BalI 246 TGGCCA TGG^CCA
774 TGGCCA
BbeI 4 GGCGCC GGCGC^C
108 GGCGCC
BbrPI 1728 CACGTG CAC^GTG
4744 CACGTG
BcgI 1689 CGAGAGCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2973 CGAAGCATCTGC
BciVI 2019 GGATAC GTATCC(6/5)
3763 GTATCC
BglI 100 GCCGAGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4419 GCCCACAGGGC
BseAI 1414 TCCGGA T^CCGGA
1662 TCCGGA
BspEI 1414 TCCGGA T^CCGGA
1662 TCCGGA
DraI 541 TTTAAA TTT^AAA
2284 TTTAAA
EagI 98 CGGCCG C^GGCCG
2531 CGGCCG
EclXI 98 CGGCCG C^GGCCG
2531 CGGCCG
Eco52I 98 CGGCCG C^GGCCG
2531 CGGCCG
EcoNI 225 CCTCCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2248 CCTACCGAAGG
FauI 130 CCCGC CCCGC(4/6)
151 CCCGC
HpaI 2793 GTTAAC GTT^AAC
3024 GTTAAC
KasI 4 GGCGCC G^GCGCC
108 GGCGCC
MluNI 246 TGGCCA TGG^CCA
774 TGGCCA
MroI 1414 TCCGGA T^CCGGA
1662 TCCGGA
MscI 246 TGGCCA TGG^CCA
774 TGGCCA
NarI 4 GGCGCC GG^CGCC
108 GGCGCC
PflMI 777 CCAAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3129 CCAGGCAGTGG
PmaCI 1728 CACGTG CAC^GTG
4744 CACGTG
PmlI 1728 CACGTG CAC^GTG
4744 CACGTG
SfoI 4 GGCGCC GGC^GCC
108 GGCGCC
SpeI 853 ACTAGT A^CTAGT
5076 ACTAGT
StuI 3658 AGGCCT AGG^CCT
4808 AGGCCT
Van91I 777 CCAAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3129 CCAGGCAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI AocI ApoI AspHI
AspI AvaI AvaII BanI BanII
BbsI BbvI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstXI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MseI MslI MspA1I
MspI MvaI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV PflFI
PleI PpuMI PstI PvuII RsaI
SacI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I