(Circular) (Six-base) MAPSORT of: pFN21AB0020.seq Check: 5959 from: 1 to: 10054 pFN21AB0020 10054 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 6335 6335 Size: 10054 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6728 7864 278 Size: 53 83 186 936 5192 1136 2468 Fragments arranged by size: 5192 2468 1136 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3009 7397 1937 Size: 1072 4388 4594 Fragments arranged by size: 4594 4388 1072 Acc65I G'GTAC_C Cuts at: 5612 7375 5612 Size: 1763 8291 AclI AA'CG_TT Cuts at: 1563 6332 8181 8554 1563 Size: 4769 1849 373 3063 Fragments arranged by size: 4769 3063 1849 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2361 2442 2753 2958 3201 3357 3801 4737 Size: 729 81 311 205 243 156 444 936 Cuts at: 4737 4806 5013 5186 5268 5282 5925 6126 6548 Size: 69 207 173 82 14 643 201 422 Cuts at: 6548 6828 8117 9501 9769 1632 Size: 280 1289 1384 268 1917 Fragments arranged by size: 1917 1384 1289 936 729 643 444 422 311 280 268 243 207 205 201 173 156 82 81 69 14 AfeI AGC'GCT Cuts at: 3925 3925 Size: 10054 AflII C'TTAA_G Cuts at: 820 1017 6490 820 Size: 197 5473 4384 Fragments arranged by size: 5473 4384 197 AflIII A'CryG_T Cuts at: 1203 3410 3734 8953 9930 1203 Size: 2207 324 5219 977 1327 Fragments arranged by size: 5219 2207 1327 977 324 AgeI A'CCGG_T Cuts at: 5180 8885 5180 Size: 3705 6349 AhdI GACnn_n'nnGTC Cuts at: 8783 8783 Size: 10054 AleI CACnn'nnGTG Cuts at: 1166 4640 5066 6529 1166 Size: 3474 426 1463 4691 Fragments arranged by size: 4691 3474 1463 426 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 10054 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5460 5492 5460 Size: 32 10022 AlwNI CAG_nnn'CTG Cuts at: 2639 3326 3956 4391 4637 4688 4946 5648 6275 Size: 687 630 435 246 51 258 702 627 Cuts at: 6275 6973 7352 7797 9369 2639 Size: 698 379 445 1572 3324 Fragments arranged by size: 3324 1572 702 698 687 630 627 445 435 379 258 246 51 ApaI G_GGCC'C Cuts at: 6435 6435 Size: 10054 ApaLI G'TGCA_C Cuts at: 6348 7259 8111 9267 6348 Size: 911 852 1156 7135 Fragments arranged by size: 7135 1156 911 852 ApoI r'AATT_y Cuts at: 1493 2549 2757 2897 6231 7363 7589 7741 9850 Size: 1056 208 140 3334 1132 226 152 2109 Cuts at: 9850 1493 Size: 1697 Fragments arranged by size: 3334 2109 1697 1132 1056 226 208 152 140 AseI AT'TA_AT Cuts at: 160 1032 8608 160 Size: 872 7576 1606 Fragments arranged by size: 7576 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 10054 AvaI C'yCGr_G Cuts at: 1870 1943 2051 2078 3722 4619 4884 7379 1870 Size: 73 108 27 1644 897 265 2495 4545 Fragments arranged by size: 4545 2495 1644 897 265 108 73 27 AvrII C'CTAG_G Cuts at: 6202 6202 Size: 10054 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4017 4050 4017 Size: 33 10021 BamHI G'GATC_C Cuts at: 4185 7384 7751 4185 Size: 3199 367 6488 Fragments arranged by size: 6488 3199 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2009 2113 4961 5177 5612 Size: 325 217 627 222 104 2848 216 435 Cuts at: 5612 7375 8830 618 Size: 1763 1455 1842 Fragments arranged by size: 2848 1842 1763 1455 627 435 325 222 217 216 104 BanII G_rGCy'C Cuts at: 1391 2021 2063 2079 2085 2226 2266 2284 3108 Size: 630 42 16 6 141 40 18 824 Cuts at: 3108 3701 3723 4656 6248 6435 7373 1391 Size: 593 22 933 1592 187 938 4072 Fragments arranged by size: 4072 1592 938 933 824 630 593 187 141 42 40 22 18 16 6 BbeI G_GCGC'C Cuts at: 2013 2117 2013 Size: 104 9950 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3027 3927 4353 4497 5730 6396 6420 Size: 971 1128 900 426 144 1233 666 24 Cuts at: 6420 928 Size: 4562 Fragments arranged by size: 4562 1233 1128 971 900 666 426 144 24 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3683 3717 4967 5001 8245 8279 1903 Size: 34 1746 34 1250 34 3244 34 3678 Fragments arranged by size: 3678 3244 1746 1250 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4018 5779 7946 9156 4018 Size: 1761 2167 1210 4916 Fragments arranged by size: 4916 2167 1761 1210 BclI T'GATC_A Cuts at: 1548 6964 1548 Size: 5416 4638 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2111 6430 8665 Size: 107 122 71 631 605 439 4319 2235 Cuts at: 8665 136 Size: 1525 Fragments arranged by size: 4319 2235 1525 631 605 439 122 107 71 BglII A'GATC_T Cuts at: 10049 10049 Size: 10054 BlpI GC'TnA_GC Cuts at: 2073 2211 2225 3751 4655 7471 2073 Size: 138 14 1526 904 2816 4656 Fragments arranged by size: 4656 2816 1526 904 138 14 Bme1580I G_kGCm'C Cuts at: 1790 3100 5630 6147 6352 6426 6435 7263 8115 Size: 1310 2530 517 205 74 9 828 852 Cuts at: 8115 9271 1790 Size: 1156 2573 Fragments arranged by size: 2573 2530 1310 1156 852 828 517 205 74 9 BmgBI CAC'GTC Cuts at: 1318 3265 1318 Size: 1947 8107 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5020 5611 7785 Size: 416 53 83 399 24 3603 591 2174 Cuts at: 7785 8743 442 Size: 958 1753 Fragments arranged by size: 3603 2174 1753 958 591 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 10054 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2077 3058 3160 3464 5136 6391 2077 Size: 981 102 304 1672 1255 5740 Fragments arranged by size: 5740 1672 1255 981 304 102 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2216 2691 4368 5117 5652 6113 8714 1239 Size: 977 475 1677 749 535 461 2601 2579 Fragments arranged by size: 2601 2579 1677 977 749 535 475 461 Bpu10I CC'TnA_GC Cuts at: 5298 5434 5298 Size: 136 9918 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2892 5106 5361 5781 5876 6098 6653 8179 9044 Size: 2214 255 420 95 222 555 1526 865 Cuts at: 9044 9342 2892 Size: 298 3604 Fragments arranged by size: 3604 2214 1526 865 555 420 298 255 222 95 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2720 4125 4423 4737 4991 6629 8717 Size: 617 1221 1405 298 314 254 1638 2088 Cuts at: 8717 882 Size: 2219 Fragments arranged by size: 2219 2088 1638 1405 1221 617 314 298 254 BsaAI yAC'GTr Cuts at: 493 1204 3735 6695 6751 7845 493 Size: 711 2531 2960 56 1094 2702 Fragments arranged by size: 2960 2702 2531 1094 711 56 BsaBI GATnn'nnATC Cuts at: 10048 10048 Size: 10054 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2010 2114 3857 6725 Size: 53 83 186 936 477 104 1743 2868 Cuts at: 6725 7861 8243 8878 275 Size: 1136 382 635 1451 Fragments arranged by size: 2868 1743 1451 1136 936 635 477 382 186 104 83 53 BsaWI w'CCGG_w Cuts at: 3419 3667 5065 5180 8487 8885 9159 9306 9717 Size: 248 1398 115 3307 398 274 147 411 Cuts at: 9717 3419 Size: 3756 Fragments arranged by size: 3756 3307 1398 411 398 274 248 147 115 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3719 3749 6848 6878 1178 Size: 30 165 30 2316 30 3099 30 4354 Fragments arranged by size: 4354 3099 2316 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2438 3164 3293 3630 3633 3636 3695 3731 Size: 1252 726 129 337 3 3 59 36 Cuts at: 3731 3740 4143 4820 5069 5364 5606 5979 6116 Size: 9 403 677 249 295 242 373 137 Cuts at: 6116 6183 6227 6296 6611 6869 7316 1186 Size: 67 44 69 315 258 447 3924 Fragments arranged by size: 3924 1252 726 677 447 403 373 337 315 295 258 249 242 137 129 69 67 59 44 36 9 3 3 BseYI C'CCAG_C Cuts at: 2084 2657 2767 4358 5166 5438 9257 9753 2084 Size: 573 110 1591 808 272 3819 496 2385 Fragments arranged by size: 3819 2385 1591 808 573 496 272 110 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3557 3782 5090 5231 5634 6804 1163 Size: 573 1821 225 1308 141 403 1170 4413 Fragments arranged by size: 4413 1821 1308 1170 573 403 225 141 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2106 4539 4899 7504 8265 9293 Size: 275 186 105 2433 360 2605 761 1028 Cuts at: 9293 1540 Size: 2301 Fragments arranged by size: 2605 2433 2301 1028 761 360 275 186 105 BsiHKAI G_wGCw'C Cuts at: 2021 2063 2226 3645 3701 3950 4656 6084 6248 Size: 42 163 1419 56 249 706 1428 164 Cuts at: 6248 6352 7263 8115 8200 9271 2021 Size: 104 911 852 85 1071 2804 Fragments arranged by size: 2804 1428 1419 1071 911 852 706 249 164 163 104 85 56 42 BsmI GAATG_Cn' Cuts at: 2801 7565 7658 2801 Size: 4764 93 5197 Fragments arranged by size: 5197 4764 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3618 4163 5264 3618 Size: 545 1101 8408 Fragments arranged by size: 8408 1101 545 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2021 2063 2079 2085 2226 2266 2284 Size: 399 231 42 16 6 141 40 18 Cuts at: 2284 3100 3108 3645 3701 3723 3950 4656 5630 Size: 816 8 537 56 22 227 706 974 Cuts at: 5630 6084 6147 6248 6352 6426 6435 7263 7373 Size: 454 63 101 104 74 9 828 110 Cuts at: 7373 8115 8200 9271 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 974 828 816 742 706 537 454 399 231 227 141 110 104 101 85 74 63 56 42 40 22 18 16 9 8 6 BspEI T'CCGG_A Cuts at: 3419 3667 9717 3419 Size: 248 6050 3756 Fragments arranged by size: 6050 3756 248 BspHI T'CATG_A Cuts at: 7943 9673 7943 Size: 1730 8324 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3199 3388 6335 6547 7409 844 Size: 2355 189 2947 212 862 3489 Fragments arranged by size: 3489 2947 2355 862 212 189 BsrBI CCG'CTC Cuts at: 1394 1823 6401 7941 1394 Size: 429 4578 1540 3507 Fragments arranged by size: 4578 3507 1540 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4128 8549 8723 65 Size: 1183 2880 4421 174 1396 Fragments arranged by size: 4421 2880 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2068 2918 3085 5180 8698 8885 1700 Size: 110 258 850 167 2095 3518 187 2869 Fragments arranged by size: 3518 2869 2095 850 258 187 167 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 10054 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 10054 BssSI C'ACGA_G Cuts at: 3101 8114 9126 3101 Size: 5013 1012 4029 Fragments arranged by size: 5013 4029 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3038 3095 4946 4988 5648 794 Size: 2244 57 1851 42 660 5200 Fragments arranged by size: 5200 2244 1851 660 57 42 BstBI TT'CG_AA Cuts at: 5847 8858 5847 Size: 3011 7043 BstEII G'GTnAC_C Cuts at: 1184 2240 1184 Size: 1056 8998 BstXI CCAn_nnnn'nTGG Cuts at: 3391 5008 5174 6776 3391 Size: 1617 166 1602 6669 Fragments arranged by size: 6669 1617 1602 166 BstYI r'GATC_y Cuts at: 1972 4185 7384 7751 8136 8153 9605 9691 9703 Size: 2213 3199 367 385 17 1452 86 12 Cuts at: 9703 10049 1972 Size: 346 1977 Fragments arranged by size: 3199 2213 1977 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3366 3714 6810 7165 3366 Size: 348 3096 355 6255 Fragments arranged by size: 6255 3096 355 348 BtgI C'CryG_G Cuts at: 513 1065 2004 2127 4539 4848 6000 513 Size: 552 939 123 2412 309 1152 4567 Fragments arranged by size: 4567 2412 1152 939 552 309 123 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2125 487 Size: 683 56 899 8416 Fragments arranged by size: 8416 899 683 56 BtsI GCAGTG_nn' Cuts at: 2793 5145 6879 7570 8364 8384 2793 Size: 2352 1734 691 794 20 4463 Fragments arranged by size: 4463 2352 1734 794 691 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 10054 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 6855 6890 529 Size: 35 6291 35 3693 Fragments arranged by size: 6291 3693 35 35 DraI TTT'AAA Cuts at: 2548 4291 7358 2548 Size: 1743 3067 5244 Fragments arranged by size: 5244 3067 1743 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4529 9061 809 Size: 507 3213 4532 1802 Fragments arranged by size: 4532 3213 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2103 2251 2779 Size: 54 1425 50 275 18 273 148 528 Cuts at: 2779 3252 4536 7501 8390 10019 8 Size: 473 1284 2965 889 1629 43 Fragments arranged by size: 2965 1629 1425 1284 889 528 473 275 273 148 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2103 4536 7501 1537 Size: 275 291 2433 2965 4090 Fragments arranged by size: 4090 2965 2433 291 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2469 2718 2808 3177 3237 3285 3984 4099 Size: 1116 249 90 369 60 48 699 115 Cuts at: 4099 4329 4989 5097 5232 6063 6102 6360 6972 Size: 230 660 108 135 831 39 258 612 Cuts at: 6972 6996 7984 1353 Size: 24 988 3423 Fragments arranged by size: 3423 1116 988 831 699 660 612 369 258 249 230 135 115 108 90 60 48 39 24 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4925 5131 8641 9015 9161 387 Size: 1064 138 3336 206 3510 374 146 1280 Fragments arranged by size: 3510 3336 1280 1064 374 206 146 138 EcoICRI GAG'CTC Cuts at: 2019 2061 2224 3699 4654 6246 2019 Size: 42 163 1475 955 1592 5827 Fragments arranged by size: 5827 1592 1475 955 163 42 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2216 2361 2442 2691 2753 2958 3201 Size: 393 584 145 81 249 62 205 243 Cuts at: 3201 3357 3801 4368 4737 4806 5013 5117 5186 Size: 156 444 567 369 69 207 104 69 Cuts at: 5186 5268 5282 5652 5925 6113 6126 6548 6828 Size: 82 14 370 273 188 13 422 280 Cuts at: 6828 8117 8714 9501 9769 1239 Size: 1289 597 787 268 1524 Fragments arranged by size: 1524 1289 787 597 584 567 444 422 393 370 369 280 273 268 249 243 207 205 188 156 145 104 82 81 69 69 62 14 13 EcoNI CCTnn'n_nnAGG Cuts at: 2234 4257 2234 Size: 2023 8031 EcoO109I rG'GnC_Cy Cuts at: 2653 3173 3427 4089 5128 6431 6805 2653 Size: 520 254 662 1039 1303 374 5902 Fragments arranged by size: 5902 1303 1039 662 520 374 254 EcoRI G'AATT_C Cuts at: 7363 7741 7363 Size: 378 9676 EcoRV GAT'ATC Cuts at: 4746 4746 Size: 10054 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4752 5216 5930 6092 6505 4752 Size: 464 714 162 413 8301 Fragments arranged by size: 8301 714 464 413 162 FspI TGC'GCA Cuts at: 8560 8560 Size: 10054 HaeII r_GCGC'y Cuts at: 1678 2013 2117 3927 9201 1678 Size: 335 104 1810 5274 2531 Fragments arranged by size: 5274 2531 1810 335 104 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3617 3649 3721 3753 3807 Size: 32 135 32 2045 32 72 32 54 Cuts at: 3807 3839 4750 4782 5591 5623 6730 6762 6848 Size: 32 911 32 809 32 1107 32 86 Cuts at: 6848 6880 7310 7342 8695 8727 8769 8801 1373 Size: 32 430 32 1353 32 42 32 2626 Fragments arranged by size: 2626 2045 1353 1107 911 809 430 135 86 72 54 42 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4800 5031 6682 6723 6867 7398 Size: 988 281 2862 231 1651 41 144 531 Cuts at: 7398 7644 669 Size: 246 3079 Fragments arranged by size: 3079 2862 1651 988 531 281 246 231 144 41 HindIII A'AGCT_T Cuts at: 748 2970 3557 3584 4044 5359 6074 748 Size: 2222 587 27 460 1315 715 4728 Fragments arranged by size: 4728 2222 1315 715 587 460 27 HpaI GTT'AAC Cuts at: 4800 5031 7644 4800 Size: 231 2613 7210 Fragments arranged by size: 7210 2613 231 KasI G'GCGC_C Cuts at: 2009 2113 2009 Size: 104 9950 KpnI G_GTAC'C Cuts at: 5616 7379 5616 Size: 1763 8291 MfeI C'AATT_G Cuts at: 6591 7653 6591 Size: 1062 8992 MluI A'CGCG_T Cuts at: 9930 9930 Size: 10054 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2305 2879 6315 6522 9168 9352 1305 Size: 1000 574 3436 207 2646 184 2007 Fragments arranged by size: 3436 2646 2007 1000 574 207 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2253 2781 10 Size: 54 1425 343 421 528 7283 Fragments arranged by size: 7283 1425 528 421 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4640 5006 5066 6063 6529 6716 8012 Size: 648 3474 366 60 997 466 187 1296 Cuts at: 8012 8371 8530 518 Size: 359 159 2042 Fragments arranged by size: 3474 2042 1296 997 648 466 366 359 187 159 60 MspA1I CmG'CkG Cuts at: 1606 2088 2129 2188 2390 3119 3335 4688 4937 Size: 482 41 59 202 729 216 1353 249 Cuts at: 4937 5170 6092 6272 6973 7470 8147 9295 9540 Size: 233 922 180 701 497 677 1148 245 Cuts at: 9540 1606 Size: 2120 Fragments arranged by size: 2120 1353 1148 922 729 701 677 497 482 249 245 233 216 202 180 59 41 NaeI GCC'GGC Cuts at: 2070 2920 3087 2070 Size: 850 167 9037 Fragments arranged by size: 9037 850 167 NarI GG'CG_CC Cuts at: 2010 2114 2010 Size: 104 9950 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 8563 Fragments arranged by size: 8563 939 552 NdeI CA'TA_TG Cuts at: 387 4674 387 Size: 4287 5767 NgoMIV G'CCGG_C Cuts at: 2068 2918 3085 2068 Size: 850 167 9037 Fragments arranged by size: 9037 850 167 NheI G'CTAG_C Cuts at: 1052 1052 Size: 10054 NotI GC'GGCC_GC Cuts at: 7501 7501 Size: 10054 NspI r_CATG'y Cuts at: 1132 1229 7412 8957 1132 Size: 97 6183 1545 2229 Fragments arranged by size: 6183 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3831 1348 Size: 2483 7571 PciI A'CATG_T Cuts at: 8953 8953 Size: 10054 PflMI CCAn_nnn'nTGG Cuts at: 1108 2788 5140 1108 Size: 1680 2352 6022 Fragments arranged by size: 6022 2352 1680 PfoI T'CCnGG_A Cuts at: 2232 4438 5691 2232 Size: 2206 1253 6595 Fragments arranged by size: 6595 2206 1253 PmeI GTTT'AAAC Cuts at: 7358 7358 Size: 10054 PmlI CAC'GTG Cuts at: 3735 6751 3735 Size: 3016 7038 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 8123 8155 9636 9668 8123 Size: 32 1481 32 8509 Fragments arranged by size: 8509 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2653 3173 3427 4089 5128 6805 2653 Size: 520 254 662 1039 1677 5902 Fragments arranged by size: 5902 1677 1039 662 520 254 PshAI GACnn'nnGTC Cuts at: 1613 3450 1613 Size: 1837 8217 PsiI TTA'TAA Cuts at: 7624 7624 Size: 10054 PspOMI G'GGCC_C Cuts at: 6431 6431 Size: 10054 PstI C_TGCA'G Cuts at: 830 2848 4942 4951 5973 6280 6346 7406 830 Size: 2018 2094 9 1022 307 66 1060 3478 Fragments arranged by size: 3478 2094 2018 1060 1022 307 66 9 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 10054 PvuII CAG'CTG Cuts at: 2188 3119 3335 4688 4937 5170 6092 6973 2188 Size: 931 216 1353 249 233 922 881 5269 Fragments arranged by size: 5269 1353 931 922 881 249 233 216 SacI G_AGCT'C Cuts at: 2021 2063 2226 3701 4656 6248 2021 Size: 42 163 1475 955 1592 5827 Fragments arranged by size: 5827 1592 1475 955 163 42 SacII CC_GC'GG Cuts at: 2130 2130 Size: 10054 SalI G'TCGA_C Cuts at: 1936 7396 1936 Size: 5460 4594 SapI GCTCTTCn'nnn_ Cuts at: 2469 3285 3984 5097 5232 2469 Size: 816 699 1113 135 7291 Fragments arranged by size: 7291 1113 816 699 135 SbfI CC_TGCA'GG Cuts at: 6346 7406 6346 Size: 1060 8994 ScaI AGT'ACT Cuts at: 8302 10014 8302 Size: 1712 8342 SfcI C'TryA_G Cuts at: 826 1045 2171 2709 2844 4566 4938 4947 5502 Size: 219 1126 538 135 1722 372 9 555 Cuts at: 5502 5865 5969 6276 6342 7402 8537 9218 9409 Size: 363 104 307 66 1060 1135 681 191 Cuts at: 9409 826 Size: 1471 Fragments arranged by size: 1722 1471 1135 1126 1060 681 555 538 372 363 307 219 191 135 104 66 9 SfoI GGC'GCC Cuts at: 2011 2115 2011 Size: 104 9950 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 10054 SgrAI Cr'CCGG_yG Cuts at: 1700 5180 1700 Size: 3480 6574 SmaI CCC'GGG Cuts at: 1872 4886 7381 1872 Size: 3014 2495 4545 Fragments arranged by size: 4545 3014 2495 SmlI C'TyrA_G Cuts at: 820 1017 1943 2907 5121 5376 5796 5855 6077 Size: 197 926 964 2214 255 420 59 222 Cuts at: 6077 6490 6632 8158 9059 9321 820 Size: 413 142 1526 901 262 1553 Fragments arranged by size: 2214 1553 1526 964 926 901 420 413 262 255 222 197 142 59 SnaBI TAC'GTA Cuts at: 493 6695 7845 493 Size: 6202 1150 2702 Fragments arranged by size: 6202 2702 1150 SpeI A'CTAG_T Cuts at: 152 2858 7081 152 Size: 2706 4223 3125 Fragments arranged by size: 4223 3125 2706 SphI G_CATG'C Cuts at: 1132 7412 1132 Size: 6280 3774 SspI AAT'ATT Cuts at: 5 52 7978 5 Size: 47 7926 2081 Fragments arranged by size: 7926 2081 47 StuI AGG'CCT Cuts at: 1517 5665 6815 1517 Size: 4148 1150 4756 Fragments arranged by size: 4756 4148 1150 StyI C'CwwG_G Cuts at: 513 1065 2004 2662 3384 4647 6202 7493 513 Size: 552 939 658 722 1263 1555 1291 3074 Fragments arranged by size: 3074 1555 1291 1263 939 722 658 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3090 4949 8081 8098 8251 8436 8881 Size: 1255 1510 1859 3132 17 153 185 445 Cuts at: 8881 325 Size: 1498 Fragments arranged by size: 3132 1859 1510 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 8300 10012 96 Size: 274 80 33 51 1443 6323 1712 138 Fragments arranged by size: 6323 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3637 3669 3719 3751 10014 10046 3637 Size: 32 50 32 6263 32 3645 Fragments arranged by size: 6263 3645 50 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2588 3914 4895 1316 Size: 1272 1326 981 6475 Fragments arranged by size: 6475 1326 1272 981 XbaI T'CTAG_A Cuts at: 5731 7390 5731 Size: 1659 8395 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 10054 XmaI C'CCGG_G Cuts at: 1870 4884 7379 1870 Size: 3014 2495 4545 Fragments arranged by size: 4545 3014 2495 XmnI GAAnn'nnTTC Cuts at: 2448 8183 2448 Size: 5735 4319 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6726 7862 276 Size: 53 83 186 936 5192 1136 2468 Fragments arranged by size: 5192 2468 1136 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI BbvCI BfrBI BsiWI BstZ17I DraIII FseI FspAI NruI NsiI PacI PspXI PsrI RsrII SanDI SexAI SfiI SrfI SwaI XcmI