Restriction map for
pFN21AB0001
(Linear) (Six-base) MAPSORT of: KIBB0001.seq Check: 1415 from: 1 to: 7533
KIBB0001.orf
With 182 enzymes: SgfI *
October 24, 2006 15:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 640 1113 1440 3233 3318 3588 4678 5553
Size: 640 473 327 1793 85 270 1090 875
Cuts at: 5553 5808 6873 7533
Size: 255 1065 660
Fragments arranged by size:
1793 1090 1065 875 660 640 473 327
270 255 85
AatII G_ACGT'C
Cuts at: 0 5972 7533
Size: 5972 1561
AccI GT'mk_AC
Cuts at: 0 4514 4697 4952 7533
Size: 4514 183 255 2581
Fragments arranged by size:
4514 2581 255 183
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 432 595 1615 2332 2344 2984 3490
Size: 181 251 163 1020 717 12 640 506
Cuts at: 3490 3547 3733 4192 4303 4348 5377 5614 7189
Size: 57 186 459 111 45 1029 237 1575
Cuts at: 7189 7533
Size: 344
Fragments arranged by size:
1575 1029 1020 717 640 506 459 344
251 237 186 181 163 111 57 45
12
AfeI AGC'GCT
Cuts at: 0 1103 1897 7533
Size: 1103 794 5636
Fragments arranged by size:
5636 1103 794
AflIII A'CryG_T
Cuts at: 0 4124 7512 7533
Size: 4124 3388 21
Fragments arranged by size:
4124 3388 21
AleI CACnn'nnGTG
Cuts at: 0 589 892 3691 5092 6763 7533
Size: 589 303 2799 1401 1671 770
Fragments arranged by size:
2799 1671 1401 770 589 303
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 557 3890 5585 6343 7345 7533
Size: 557 3333 1695 758 1002 188
Fragments arranged by size:
3333 1695 1002 758 557 188
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 6643 6675 7533
Size: 6643 32 858
Fragments arranged by size:
6643 858 32
AlwNI CAG_nnn'CTG
Cuts at: 0 912 1140 1308 1341 1611 3195 3441 3468
Size: 912 228 168 33 270 1584 246 27
Cuts at: 3468 3531 3603 3837 3904 5709 6495 6567 7230
Size: 63 72 234 67 1805 786 72 663
Cuts at: 7230 7245 7533
Size: 15 288
Fragments arranged by size:
1805 1584 912 786 663 288 270 246
234 228 168 72 72 67 63 33
27 15
ApaI G_GGCC'C
Cuts at: 0 458 1062 3501 5502 5856 5891 6685 6901
Size: 458 604 2439 2001 354 35 794 216
Cuts at: 6901 7533
Size: 632
Fragments arranged by size:
2439 2001 794 632 604 458 354 216
35
ApaLI G'TGCA_C
Cuts at: 0 1198 5481 6739 6816 7533
Size: 1198 4283 1258 77 717
Fragments arranged by size:
4283 1258 1198 717 77
ApoI r'AATT_y
Cuts at: 0 5311 7533
Size: 5311 2222
AvaI C'yCGr_G
Cuts at: 0 370 622 926 1005 1905 1953 2152 2471
Size: 370 252 304 79 900 48 199 319
Cuts at: 2471 3048 3509 3964 4057 4350 4461 4610 4809
Size: 577 461 455 93 293 111 149 199
Cuts at: 4809 4999 5022 5028 5168 5855 6017 6821 6892
Size: 190 23 6 140 687 162 804 71
Cuts at: 6892 7533
Size: 641
Fragments arranged by size:
900 804 687 641 577 461 455 370
319 304 293 252 199 199 190 162
149 140 111 93 79 71 48 23
6
BamHI G'GATC_C
Cuts at: 0 3891 5756 7533
Size: 3891 1865 1777
Fragments arranged by size:
3891 1865 1777
BanI G'GyrC_C
Cuts at: 0 628 748 886 989 1452 1852 1914 3330
Size: 628 120 138 103 463 400 62 1416
Cuts at: 3330 3377 3480 4049 4678 4848 4941 5798 5992
Size: 47 103 569 629 170 93 857 194
Cuts at: 5992 6181 7354 7533
Size: 189 1173 179
Fragments arranged by size:
1416 1173 857 629 628 569 463 400
194 189 179 170 138 120 103 103
93 62 47
BanII G_rGCy'C
Cuts at: 0 131 458 933 1055 1062 1192 2093 2224
Size: 131 327 475 122 7 130 901 131
Cuts at: 2224 2945 3121 3290 3501 4391 4468 5029 5502
Size: 721 176 169 211 890 77 561 473
Cuts at: 5502 5856 5891 6509 6685 6718 6757 6901 6979
Size: 354 35 618 176 33 39 144 78
Cuts at: 6979 7533
Size: 554
Fragments arranged by size:
901 890 721 618 561 554 475 473
354 327 211 176 176 169 144 131
131 130 122 78 77 39 35 33
7
BbeI G_GCGC'C
Cuts at: 0 1856 4682 5802 6185 7358 7533
Size: 1856 2826 1120 383 1173 175
Fragments arranged by size:
2826 1856 1173 1120 383 175
BbsI GAAGACnn'nnnn_
Cuts at: 0 2695 4310 5076 5895 6045 7533
Size: 2695 1615 766 819 150 1488
Fragments arranged by size:
2695 1615 1488 819 766 150
BbvCI CC'TCA_GC
Cuts at: 0 230 1714 1993 2571 3250 3978 4429 7082
Size: 230 1484 279 578 679 728 451 2653
Cuts at: 7082 7533
Size: 451
Fragments arranged by size:
2653 1484 728 679 578 451 451 279
230
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 624 658 917 951 5139 5173 5392 5426
Size: 624 34 259 34 4188 34 219 34
Cuts at: 5426 5538 5572 6008 6042 6403 6437 7533
Size: 112 34 436 34 361 34 1096
Fragments arranged by size:
4188 1096 624 436 361 259 219 112
34 34 34 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 7425 7533
Size: 7425 108
BclI T'GATC_A
Cuts at: 0 7208 7533
Size: 7208 325
BglI GCCn_nnn'nGGC
Cuts at: 0 517 626 844 1907 3223 3841 4166 4323
Size: 517 109 218 1063 1316 618 325 157
Cuts at: 4323 4534 5875 6161 6937 7226 7533
Size: 211 1341 286 776 289 307
Fragments arranged by size:
1341 1316 1063 776 618 517 325 307
289 286 218 211 157 109
BlpI GC'TnA_GC
Cuts at: 0 616 1522 1615 1699 2137 3643 3778 3802
Size: 616 906 93 84 438 1506 135 24
Cuts at: 3802 6790 7246 7289 7505 7533
Size: 2988 456 43 216 28
Fragments arranged by size:
2988 1506 906 616 456 438 216 135
93 84 43 28 24
Bme1580I G_kGCm'C
Cuts at: 0 458 541 631 992 1062 1202 1457 1501
Size: 458 83 90 361 70 140 255 44
Cuts at: 1501 1919 3335 3485 3501 4052 5485 5502 5856
Size: 418 1416 150 16 551 1433 17 354
Cuts at: 5856 5891 6031 6685 6743 6820 6901 7533
Size: 35 140 654 58 77 81 632
Fragments arranged by size:
1433 1416 654 632 551 458 418 361
354 255 150 140 140 90 83 81
77 70 58 44 35 17 16
BmgBI CAC'GTC
Cuts at: 0 2186 2529 3231 4446 6195 7425 7533
Size: 2186 343 702 1215 1749 1230 108
Fragments arranged by size:
2186 1749 1230 1215 702 343 108
BmrI ACTGGGnnnn_n'
Cuts at: 0 2154 2792 3084 7533
Size: 2154 638 292 4449
Fragments arranged by size:
4449 2154 638 292
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1020 4953 6588 7533
Size: 1020 3933 1635 945
Fragments arranged by size:
3933 1635 1020 945
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 471 1063 1333 1833 2296 2398 2818 2833
Size: 471 592 270 500 463 102 420 15
Cuts at: 2833 3046 3361 3607 3859 4128 4576 4945 5107
Size: 213 315 246 252 269 448 369 162
Cuts at: 5107 5713 5866 5959 6295 6517 7171 7474 7489
Size: 606 153 93 336 222 654 303 15
Cuts at: 7489 7533
Size: 44
Fragments arranged by size:
654 606 592 500 471 463 448 420
369 336 315 303 270 269 252 246
222 213 162 153 102 93 44 15
15
Bpu10I CC'TnA_GC
Cuts at: 0 230 358 1351 1714 1993 2258 2571 2674
Size: 230 128 993 363 279 265 313 103
Cuts at: 2674 3250 3978 4114 4429 6032 6316 7082 7533
Size: 576 728 136 315 1603 284 766 451
Fragments arranged by size:
1603 993 766 728 576 451 363 315
313 284 279 265 230 136 128 103
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 621 2077 3655 5461 5943 7533
Size: 621 1456 1578 1806 482 1590
Fragments arranged by size:
1806 1590 1578 1456 621 482
BsaI GGTCTCn'nnnn_
Cuts at: 0 337 359 4490 7533
Size: 337 22 4131 3043
Fragments arranged by size:
4131 3043 337 22
BsaAI yAC'GTr
Cuts at: 0 2253 7029 7533
Size: 2253 4776 504
Fragments arranged by size:
4776 2253 504
BsaBI GATnn'nnATC
Cuts at: 0 3109 7533
Size: 3109 4424
BsaHI Gr'CG_yC
Cuts at: 0 317 1853 2426 2717 3272 4679 4820 5513
Size: 317 1536 573 291 555 1407 141 693
Cuts at: 5513 5799 5900 5969 6182 6265 7355 7533
Size: 286 101 69 213 83 1090 178
Fragments arranged by size:
1536 1407 1090 693 573 555 317 291
286 213 178 141 101 83 69
BsaWI w'CCGG_w
Cuts at: 0 4406 4887 5759 6551 7533
Size: 4406 481 872 792 982
Fragments arranged by size:
4406 982 872 792 481
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 415 445 1993 2023 2230 2260 2584 2614
Size: 415 30 1548 30 207 30 324 30
Cuts at: 2614 3361 3391 4450 4480 4487 4517 4609 4639
Size: 747 30 1059 30 7 30 92 30
Cuts at: 4639 4690 4720 6533 6563 6643 6673 7375 7405
Size: 51 30 1813 30 80 30 702 30
Cuts at: 7405 7533
Size: 128
Fragments arranged by size:
1813 1548 1059 747 702 415 324 207
128 92 80 51 30 30 30 30
30 30 30 30 30 30 30 30
7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 19 474 1804 1897 3385 3421 4573 4588
Size: 19 455 1330 93 1488 36 1152 15
Cuts at: 4588 4635 4942 5590 6048 7195 7533
Size: 47 307 648 458 1147 338
Fragments arranged by size:
1488 1330 1152 1147 648 458 455 338
307 93 47 36 19 15
BseYI C'CCAG_C
Cuts at: 0 457 606 1098 1416 2463 3458 3706 3910
Size: 457 149 492 318 1047 995 248 204
Cuts at: 3910 4268 4634 4661 4682 5138 6254 6632 6956
Size: 358 366 27 21 456 1116 378 324
Cuts at: 6956 6968 6978 7533
Size: 12 10 555
Fragments arranged by size:
1116 1047 995 555 492 457 456 378
366 358 324 318 248 204 149 27
21 12 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 613 1054 1321 1465 2319 2392 2740 3454
Size: 613 441 267 144 854 73 348 714
Cuts at: 3454 4015 4674 5461 5581 5746 5836 6193 6730
Size: 561 659 787 120 165 90 357 537
Cuts at: 6730 6907 7135 7533
Size: 177 228 398
Fragments arranged by size:
854 787 714 659 613 561 537 441
398 357 348 267 228 177 165 144
120 90 73
BsiEI CG_ry'CG
Cuts at: 0 839 2537 2646 3822 5292 6236 7533
Size: 839 1698 109 1176 1470 944 1297
Fragments arranged by size:
1698 1470 1297 1176 944 839 109
BsiHKAI G_wGCw'C
Cuts at: 0 1049 1202 1621 1633 2093 2264 4603 5485
Size: 1049 153 419 12 460 171 2339 882
Cuts at: 5485 6223 6509 6718 6743 6820 6955 7139 7533
Size: 738 286 209 25 77 135 184 394
Fragments arranged by size:
2339 1049 882 738 460 419 394 286
209 184 171 153 135 77 25 12
BsmBI CGTCTCn'nnnn_
Cuts at: 0 4811 4999 6205 7084 7533
Size: 4811 188 1206 879 449
Fragments arranged by size:
4811 1206 879 449 188
Bsp1286I G_dGCh'C
Cuts at: 0 131 458 541 631 933 992 1049 1055
Size: 131 327 83 90 302 59 57 6
Cuts at: 1055 1062 1192 1202 1457 1501 1621 1633 1919
Size: 7 130 10 255 44 120 12 286
Cuts at: 1919 2093 2224 2264 2945 3121 3290 3335 3485
Size: 174 131 40 681 176 169 45 150
Cuts at: 3485 3501 4052 4391 4468 4603 5029 5485 5502
Size: 16 551 339 77 135 426 456 17
Cuts at: 5502 5856 5891 6031 6223 6509 6685 6718 6743
Size: 354 35 140 192 286 176 33 25
Cuts at: 6743 6757 6820 6901 6955 6979 7139 7533
Size: 14 63 81 54 24 160 394
Fragments arranged by size:
681 551 456 426 394 354 339 327
302 286 286 255 192 176 176 174
169 160 150 140 135 131 131 130
120 90 83 81 77 63 59 57
54 45 44 40 35 33 25 24
17 16 14 12 10 7 6
BspEI T'CCGG_A
Cuts at: 0 5759 6551 7533
Size: 5759 792 982
Fragments arranged by size:
5759 982 792
BspHI T'CATG_A
Cuts at: 0 6470 7533
Size: 6470 1063
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 640 1076 1113 1440 1703 3149 3233 3318
Size: 640 436 37 327 263 1446 84 85
Cuts at: 3318 3587 3588 4678 5553 5772 5808 6662 6873
Size: 269 1 1090 875 219 36 854 211
Cuts at: 6873 7533
Size: 660
Fragments arranged by size:
1446 1090 875 854 660 640 436 327
269 263 219 211 85 84 37 36
1
BsrBI CCG'CTC
Cuts at: 0 335 671 821 1473 5337 6723 7533
Size: 335 336 150 652 3864 1386 810
Fragments arranged by size:
3864 1386 810 652 336 335 150
BsrDI GCAATG_nn'
Cuts at: 0 5864 6087 7533
Size: 5864 223 1446
Fragments arranged by size:
5864 1446 223
BsrFI r'CCGG_y
Cuts at: 0 19 425 745 2029 2392 2532 3739 4211
Size: 19 406 320 1284 363 140 1207 472
Cuts at: 4211 4245 5446 5789 6143 6913 7533
Size: 34 1201 343 354 770 620
Fragments arranged by size:
1284 1207 1201 770 620 472 406 363
354 343 320 140 34 19
BsrGI T'GTAC_A
Cuts at: 0 3197 7533
Size: 3197 4336
BssHII G'CGCG_C
Cuts at: 0 3390 7338 7533
Size: 3390 3948 195
Fragments arranged by size:
3948 3390 195
BssSI C'ACGA_G
Cuts at: 0 379 5734 7533
Size: 379 5355 1799
Fragments arranged by size:
5355 1799 379
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1308 1452 3330 3441 3531 3904 4038 5193
Size: 1308 144 1878 111 90 373 134 1155
Cuts at: 5193 7533
Size: 2340
Fragments arranged by size:
2340 1878 1308 1155 373 144 134 111
90
BstEII G'GTnAC_C
Cuts at: 0 3453 3748 6012 7435 7533
Size: 3453 295 2264 1423 98
Fragments arranged by size:
3453 2264 1423 295 98
BstXI CCAn_nnnn'nTGG
Cuts at: 0 495 3918 4772 4863 6597 7533
Size: 495 3423 854 91 1734 936
Fragments arranged by size:
3423 1734 936 854 495 91
BstYI r'GATC_y
Cuts at: 0 1094 3503 3891 5756 7533
Size: 1094 2409 388 1865 1777
Fragments arranged by size:
2409 1865 1777 1094 388
Bsu36I CC'TnA_GG
Cuts at: 0 792 1210 1292 1961 4571 7533
Size: 792 418 82 669 2610 2962
Fragments arranged by size:
2962 2610 792 669 418 82
BtgI C'CryG_G
Cuts at: 0 876 1149 1401 1856 1865 2936 3307 3692
Size: 876 273 252 455 9 1071 371 385
Cuts at: 3692 3744 5093 5373 5493 5891 5915 6119 6389
Size: 52 1349 280 120 398 24 204 270
Cuts at: 6389 7199 7317 7533
Size: 810 118 216
Fragments arranged by size:
1349 1071 876 810 455 398 385 371
280 273 270 252 216 204 120 118
52 24 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 427 447 1353 2052 5037 5043 5649 7533
Size: 427 20 906 699 2985 6 606 1884
Fragments arranged by size:
2985 1884 906 699 606 427 20 6
BtsI GCAGTG_nn'
Cuts at: 0 541 2077 3202 3439 3686 4036 5415 5594
Size: 541 1536 1125 237 247 350 1379 179
Cuts at: 5594 5710 6181 6820 7533
Size: 116 471 639 713
Fragments arranged by size:
1536 1379 1125 713 639 541 471 350
247 237 179 116
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 7332 7367 7533
Size: 7332 35 166
Fragments arranged by size:
7332 166 35
DraIII CAC_nnn'GTG
Cuts at: 0 1505 2268 7533
Size: 1505 763 5265
Fragments arranged by size:
5265 1505 763
DrdI GACnn_nn'nnGTC
Cuts at: 0 705 2647 6235 7533
Size: 705 1942 3588 1298
Fragments arranged by size:
3588 1942 1298 705
EaeI y'GGCC_r
Cuts at: 0 419 686 836 1022 1734 2390 2534 3122
Size: 419 267 150 186 712 656 144 588
Cuts at: 3122 3263 3737 3741 3812 4101 4733 4865 5621
Size: 141 474 4 71 289 632 132 756
Cuts at: 5621 5912 6167 6911 7533
Size: 291 255 744 622
Fragments arranged by size:
756 744 712 656 632 622 588 474
419 291 289 267 255 186 150 144
141 132 71 4
EagI C'GGCC_G
Cuts at: 0 836 2534 7533
Size: 836 1698 4999
Fragments arranged by size:
4999 1698 836
EarI CTCTTCn'nnn_
Cuts at: 0 2659 2908 2953 4144 4204 4314 5243 6295
Size: 2659 249 45 1191 60 110 929 1052
Cuts at: 6295 7006 7533
Size: 711 527
Fragments arranged by size:
2659 1191 1052 929 711 527 249 110
60 45
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2181 4369 4531 5260 5776 6105 6463 6595
Size: 2181 2188 162 729 516 329 358 132
Cuts at: 6595 7533
Size: 938
Fragments arranged by size:
2188 2181 938 729 516 358 329 162
132
EcoICRI GAG'CTC
Cuts at: 0 2091 6507 6716 7533
Size: 2091 4416 209 817
Fragments arranged by size:
4416 2091 817 209
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 432 471 595 1063 1333 1615 1833
Size: 181 251 39 124 468 270 282 218
Cuts at: 1833 2296 2332 2344 2398 2818 2833 2984 3046
Size: 463 36 12 54 420 15 151 62
Cuts at: 3046 3361 3490 3547 3607 3733 3859 4128 4192
Size: 315 129 57 60 126 126 269 64
Cuts at: 4192 4303 4348 4576 4945 5107 5377 5614 5713
Size: 111 45 228 369 162 270 237 99
Cuts at: 5713 5866 5959 6295 6517 7171 7189 7474 7489
Size: 153 93 336 222 654 18 285 15
Cuts at: 7489 7533
Size: 44
Fragments arranged by size:
654 468 463 420 369 336 315 285
282 270 270 269 251 237 228 222
218 181 162 153 151 129 126 126
124 111 99 93 64 62 60 57
54 45 44 39 36 18 15 15
12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1024 1747 1783 1812 2299 2956 3403 4117
Size: 1024 723 36 29 487 657 447 714
Cuts at: 4117 4555 6382 7393 7533
Size: 438 1827 1011 140
Fragments arranged by size:
1827 1024 1011 723 714 657 487 447
438 140 36 29
EcoO109I rG'GnC_Cy
Cuts at: 0 165 354 454 1059 1320 1338 1957 1965
Size: 165 189 100 605 261 18 619 8
Cuts at: 1965 2241 3497 3513 3633 3834 3969 4526 4617
Size: 276 1256 16 120 201 135 557 91
Cuts at: 4617 4860 5379 5498 5852 5859 6375 6681 6682
Size: 243 519 119 354 7 516 306 1
Cuts at: 6682 6897 6898 6929 7173 7227 7368 7533
Size: 215 1 31 244 54 141 165
Fragments arranged by size:
1256 619 605 557 519 516 354 306
276 261 244 243 215 201 189 165
165 141 135 120 119 100 91 54
31 18 16 8 7 1 1
EcoRV GAT'ATC
Cuts at: 0 801 7533
Size: 801 6732
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3369 4197 7533
Size: 3369 828 3336
Fragments arranged by size:
3369 3336 828
FseI GG_CCGG'CC
Cuts at: 0 3743 7533
Size: 3743 3790
HaeII r_GCGC'y
Cuts at: 0 1105 1470 1856 1899 3917 3929 4682 5437
Size: 1105 365 386 43 2018 12 753 755
Cuts at: 5437 5802 6185 7159 7358 7533
Size: 365 383 974 199 175
Fragments arranged by size:
2018 1105 974 755 753 386 383 365
365 199 175 43 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 213 245 1541 1573 1811 1843 2584 2616
Size: 213 32 1296 32 238 32 741 32
Cuts at: 2616 3961 3993 4291 4323 4450 4482 4609 4641
Size: 1345 32 298 32 127 32 127 32
Cuts at: 4641 7533
Size: 2892
Fragments arranged by size:
2892 1345 1296 741 298 238 213 127
127 32 32 32 32 32 32 32
32
HincII GTy'rAC
Cuts at: 0 2208 5223 5741 6429 7533
Size: 2208 3015 518 688 1104
Fragments arranged by size:
3015 2208 1104 688 518
KasI G'GCGC_C
Cuts at: 0 1852 4678 5798 6181 7354 7533
Size: 1852 2826 1120 383 1173 179
Fragments arranged by size:
2826 1852 1173 1120 383 179
MluI A'CGCG_T
Cuts at: 0 4124 7512 7533
Size: 4124 3388 21
Fragments arranged by size:
4124 3388 21
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 26 1004 1088 1217 3365 4448 4937 7533
Size: 26 978 84 129 2148 1083 489 2596
Fragments arranged by size:
2596 2148 1083 978 489 129 84 26
MscI TGG'CCA
Cuts at: 0 421 1024 4103 4735 4867 7533
Size: 421 603 3079 632 132 2666
Fragments arranged by size:
3079 2666 632 603 421 132
MslI CAynn'nnrTG
Cuts at: 0 589 892 1390 3691 5092 6205 6475 6691
Size: 589 303 498 2301 1401 1113 270 216
Cuts at: 6691 6763 7129 7533
Size: 72 366 404
Fragments arranged by size:
2301 1401 1113 589 498 404 366 303
270 216 72
MspA1I CmG'CkG
Cuts at: 0 234 530 652 957 1280 1459 1608 1992
Size: 234 296 122 305 323 179 149 384
Cuts at: 1992 2132 2727 3309 3585 3647 3801 4268 4634
Size: 140 595 582 276 62 154 467 366
Cuts at: 4634 4826 5990 6372 6648 6960 7167 7288 7463
Size: 192 1164 382 276 312 207 121 175
Cuts at: 7463 7533
Size: 70
Fragments arranged by size:
1164 595 582 467 384 382 366 323
312 305 296 276 276 234 207 192
179 175 154 149 140 122 121 70
62
NaeI GCC'GGC
Cuts at: 0 21 747 2394 2534 3741 4213 5448 6145
Size: 21 726 1647 140 1207 472 1235 697
Cuts at: 6145 6915 7533
Size: 770 618
Fragments arranged by size:
1647 1235 1207 770 726 697 618 472
140 21
NarI GG'CG_CC
Cuts at: 0 1853 4679 5799 6182 7355 7533
Size: 1853 2826 1120 383 1173 178
Fragments arranged by size:
2826 1853 1173 1120 383 178
NcoI C'CATG_G
Cuts at: 0 1856 5493 6119 7317 7533
Size: 1856 3637 626 1198 216
Fragments arranged by size:
3637 1856 1198 626 216
NdeI CA'TA_TG
Cuts at: 0 7102 7533
Size: 7102 431
NgoMIV G'CCGG_C
Cuts at: 0 19 745 2392 2532 3739 4211 5446 6143
Size: 19 726 1647 140 1207 472 1235 697
Cuts at: 6143 6913 7533
Size: 770 620
Fragments arranged by size:
1647 1235 1207 770 726 697 620 472
140 19
NspI r_CATG'y
Cuts at: 0 27 1252 1359 3705 4591 5194 6605 7533
Size: 27 1225 107 2346 886 603 1411 928
Fragments arranged by size:
2346 1411 1225 928 886 603 107 27
PasI CC'CwG_GG
Cuts at: 0 1743 2706 3879 3918 4765 7533
Size: 1743 963 1173 39 847 2768
Fragments arranged by size:
2768 1743 1173 963 847 39
PflMI CCAn_nnn'nTGG
Cuts at: 0 1341 1743 2517 2622 3837 4634 6261 6495
Size: 1341 402 774 105 1215 797 1627 234
Cuts at: 6495 6777 6852 7230 7533
Size: 282 75 378 303
Fragments arranged by size:
1627 1341 1215 797 774 402 378 303
282 234 105 75
PfoI T'CCnGG_A
Cuts at: 0 28 803 2105 2234 2810 3023 3338 3653
Size: 28 775 1302 129 576 213 315 315
Cuts at: 3653 4448 5297 5453 5752 5963 7533
Size: 795 849 156 299 211 1570
Fragments arranged by size:
1570 1302 849 795 775 576 315 315
299 213 211 156 129 28
PmlI CAC'GTG
Cuts at: 0 2253 7533
Size: 2253 5280
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 6643 6675 7533
Size: 6643 32 858
Fragments arranged by size:
6643 858 32
PpuMI rG'GwC_Cy
Cuts at: 0 165 1338 1957 3513 3633 3969 4617 5379
Size: 165 1173 619 1556 120 336 648 762
Cuts at: 5379 6375 7368 7533
Size: 996 993 165
Fragments arranged by size:
1556 1173 996 993 762 648 619 336
165 165 120
PspOMI G'GGCC_C
Cuts at: 0 454 1058 3497 5498 5852 5887 6681 6897
Size: 454 604 2439 2001 354 35 794 216
Cuts at: 6897 7533
Size: 636
Fragments arranged by size:
2439 2001 794 636 604 454 354 216
35
PstI C_TGCA'G
Cuts at: 0 506 1124 2087 2303 3104 3209 3404 3446
Size: 506 618 963 216 801 105 195 42
Cuts at: 3446 3559 3584 3800 3863 4481 5261 6563 6815
Size: 113 25 216 63 618 780 1302 252
Cuts at: 6815 6965 7533
Size: 150 568
Fragments arranged by size:
1302 963 801 780 618 618 568 506
252 216 216 195 150 113 105 63
42 25
PvuI CG_AT'CG
Cuts at: 0 6236 7533
Size: 6236 1297
PvuII CAG'CTG
Cuts at: 0 234 1608 1992 2727 3585 3801 6648 6960
Size: 234 1374 384 735 858 216 2847 312
Cuts at: 6960 7533
Size: 573
Fragments arranged by size:
2847 1374 858 735 573 384 312 234
216
RsrII CG'GwC_CG
Cuts at: 0 149 2027 7533
Size: 149 1878 5506
Fragments arranged by size:
5506 1878 149
SacI G_AGCT'C
Cuts at: 0 2093 6509 6718 7533
Size: 2093 4416 209 815
Fragments arranged by size:
4416 2093 815 209
SacII CC_GC'GG
Cuts at: 0 3310 7533
Size: 3310 4223
SanDI GG'GwC_CC
Cuts at: 0 3513 3969 7533
Size: 3513 456 3564
Fragments arranged by size:
3564 3513 456
SapI GCTCTTCn'nnn_
Cuts at: 0 2908 4204 7533
Size: 2908 1296 3329
Fragments arranged by size:
3329 2908 1296
SbfI CC_TGCA'GG
Cuts at: 0 3404 3559 7533
Size: 3404 155 3974
Fragments arranged by size:
3974 3404 155
SexAI A'CCwGG_T
Cuts at: 0 2213 2621 2852 4499 5225 6377 6512 7533
Size: 2213 408 231 1647 726 1152 135 1021
Fragments arranged by size:
2213 1647 1152 1021 726 408 231 135
SfcI C'TryA_G
Cuts at: 0 387 502 1120 2083 2299 3100 3205 3400
Size: 387 115 618 963 216 801 105 195
Cuts at: 3400 3442 3555 3580 3796 3859 4477 4953 5257
Size: 42 113 25 216 63 618 476 304
Cuts at: 5257 6351 6559 6811 6961 7533
Size: 1094 208 252 150 572
Fragments arranged by size:
1094 963 801 618 618 572 476 387
304 252 216 216 208 195 150 115
113 105 63 42 25
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 844 6161 6937 7533
Size: 844 5317 776 596
Fragments arranged by size:
5317 844 776 596
SfoI GGC'GCC
Cuts at: 0 1854 4680 5800 6183 7356 7533
Size: 1854 2826 1120 383 1173 177
Fragments arranged by size:
2826 1854 1173 1120 383 177
SgrAI Cr'CCGG_yG
Cuts at: 0 5789 7533
Size: 5789 1744
SmaI CCC'GGG
Cuts at: 0 1907 3511 4463 4612 5001 5170 5857 7533
Size: 1907 1604 952 149 389 169 687 1676
Fragments arranged by size:
1907 1676 1604 952 687 389 169 149
SmlI C'TyrA_G
Cuts at: 0 600 2092 3670 5476 5922 7533
Size: 600 1492 1578 1806 446 1611
Fragments arranged by size:
1806 1611 1578 1492 600 446
SnaBI TAC'GTA
Cuts at: 0 7029 7533
Size: 7029 504
SphI G_CATG'C
Cuts at: 0 1359 6605 7533
Size: 1359 5246 928
Fragments arranged by size:
5246 1359 928
StuI AGG'CCT
Cuts at: 0 640 1685 2818 3676 3773 6155 6164 7533
Size: 640 1045 1133 858 97 2382 9 1369
Fragments arranged by size:
2382 1369 1133 1045 858 640 97 9
StyI C'CwwG_G
Cuts at: 0 890 1025 1856 1934 2015 3449 4856 5240
Size: 890 135 831 78 81 1434 1407 384
Cuts at: 5240 5493 5532 6119 6614 7304 7317 7533
Size: 253 39 587 495 690 13 216
Fragments arranged by size:
1434 1407 890 831 690 587 495 384
253 216 135 81 78 39 13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 312 1408 3066 3196 4260 5668 6758 7003
Size: 312 1096 1658 130 1064 1408 1090 245
Cuts at: 7003 7057 7317 7533
Size: 54 260 216
Fragments arranged by size:
1658 1408 1096 1090 1064 312 260 245
216 130 54
TatI w'GTAC_w
Cuts at: 0 2198 3197 5984 7295 7533
Size: 2198 999 2787 1311 238
Fragments arranged by size:
2787 2198 1311 999 238
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1989 2021 2230 2262 3361 3393 3742 3774
Size: 1989 32 209 32 1099 32 349 32
Cuts at: 3774 4487 4519 4877 4909 7533
Size: 713 32 358 32 2624
Fragments arranged by size:
2624 1989 1099 713 358 349 209 32
32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 94 1645 1942 2790 5227 7533
Size: 94 1551 297 848 2437 2306
Fragments arranged by size:
2437 2306 1551 848 297 94
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 465 708 913 981 1863 2501 4353 4920
Size: 465 243 205 68 882 638 1852 567
Cuts at: 4920 6117 6192 7029 7533
Size: 1197 75 837 504
Fragments arranged by size:
1852 1197 882 837 638 567 504 465
243 205 75 68
XmaI C'CCGG_G
Cuts at: 0 1905 3509 4461 4610 4999 5168 5855 7533
Size: 1905 1604 952 149 389 169 687 1678
Fragments arranged by size:
1905 1678 1604 952 687 389 169 149
XmnI GAAnn'nnTTC
Cuts at: 0 1585 4180 7533
Size: 1585 2595 3353
Fragments arranged by size:
3353 2595 1585
ZraI GAC'GTC
Cuts at: 0 5970 7533
Size: 5970 1563
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflIII AleI AlfI
AloI AlwNI ApaI ApaLI ApoI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRV FalI FseI HaeII Hin4I HincII KasI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NspI PasI PflMI PfoI PmlI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SacII
SanDI SapI SbfI SexAI SfcI SfiI SfoI SgrAI
SmaI SmlI SnaBI SphI StuI StyI TaqII TatI
TstI Tth111I XcmI XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AflII AgeI AhdI AscI AseI AsiSI
AvrII BaeI BfrBI BglII BmtI BsiWI BsmI BstBI
BstZ17I ClaI DraI EcoRI FspI FspAI HindIII HpaI
KpnI MfeI NheI NotI NruI NsiI PacI PciI
PmeI PshAI PsiI PspXI PsrI SalI ScaI SgfI
SpeI SrfI SspI SwaI XbaI XhoI