Restriction map for
pFN21AA2034
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# NO CUT ENZYMES ( 59 enzymes) #
##################################
Acc65I AclI AflII Alw44I Aor51HI
ApaLI AscI AseI AsnI Asp718I
AvrII BamHI BclI BfrI BlnI
BsaBI BsiWI BsmI Bsp1407I BspDI
BspHI BspLU11I BsrGI Bst1107I BstBI
BstZ17I ClaI DraI Eco47III EcoRV
EcoT22I FbaI HindIII HpaI KpnI
MamI MluI NdeI NheI NotI
NruI NsiI NspV PacI PmeI
PshAI PshBI Psp1406I PvuI RcaI
ScaI SfuI SgrAI SnaBI SpeI
SspBI SspI SwaI XbaI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AatII 4738 GACGTC GACGT^C
AccI 1831 GTCGAC GT^[AC][GT]AC
AccIII 2123 TCCGGA T^CCGGA
AgeI 1004 ACCGGT A^CCGGT
Asp700I 1485 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 5705 TGCGCA TGC^GCA
BcgI 1778 CGAGGCACCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2569 GGATAC GTATCC(6/5)
BseAI 2123 TCCGGA T^CCGGA
BspEI 2123 TCCGGA T^CCGGA
BssHII 2838 GCGCGC G^CGCGC
BstXI 4472 CCATGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CpoI 5270 CGGACCG CG^G[AT]CCG
EcoRI 3153 GAATTC G^AATTC
FspI 5705 TGCGCA TGC^GCA
HincII 1831 GTCGAC GT[CT]^[GA]AC
HindII 1831 GTCGAC GT[CT]^[GA]AC
MfeI 2857 CAATTG C^AATTG
MroI 2123 TCCGGA T^CCGGA
MseI 5726 TTAA T^TAA
MunI 2857 CAATTG C^AATTG
NspI 551 GCATGC [GA]CATG^[CT]
PaeR7I 985 CTCGAG C^TCGAG
PinAI 1004 ACCGGT A^CCGGT
RsrII 5270 CGGACCG CG^G[AT]CCG
SalI 1831 GTCGAC G^TCGAC
SexAI 2094 ACCAGGT A^CC[AT]GGT
SfiI 3855 GGCCCTGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 551 GCATGC GCATG^C
Tru9I 5726 TTAA T^TAA
XhoI 985 CTCGAG C^TCGAG
XmnI 1485 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 19 enzymes) #
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AflIII 404 ACACGT A^C[GA][CT]GT
518 ACGTGT
AhdI 3550 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3906 GACTGCACGTC
AocI 2619 CCTGAGG CC^T[ACGT]AGG
6079 CCTGAGG
AspEI 3550 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3906 GACTGCACGTC
AspI 12 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1882 GACAACGTC
BstEII 2529 GGTCACC G^GT[ACGT]ACC
5332 GGTAACC
BstPI 2529 GGTCACC G^GT[ACGT]ACC
5332 GGTAACC
Bsu36I 2619 CCTGAGG CC^T[ACGT]AGG
6079 CCTGAGG
DraIII 502 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG
2093 CACCAGGTG
Eam1105I 3550 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3906 GACTGCACGTC
Eco81I 2619 CCTGAGG CC^T[ACGT]AGG
6079 CCTGAGG
EcoO65I 2529 GGTCACC G^GT[ACGT]ACC
5332 GGTAACC
FseI 1652 GGCCGGCC GGCCGG^CC
5865 GGCCGGCC
NcoI 4193 CCATGG C^CATGG
4472 CCATGG
PflFI 12 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1882 GACAACGTC
SapI 5401 GAAGAGC GCTCTTC(1/4)
1536 GCTCTTC
SbfI 4911 CCTGCAGG CCTGCA^GG
5463 CCTGCAGG
Sse8387I 4911 CCTGCAGG CCTGCA^GG
5463 CCTGCAGG
Tth111I 12 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1882 GACAACGTC
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# 3 OR MORE CUT ENZYMES ( 148 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApaI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbeI BbrPI BbsI BbvCI
BbvI BcnI BfaI BglI BglII
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrDI BsrFI
BsrI BssKI BssSI BstAPI BstF5I
BstNI BstUI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DrdI DsaI EaeI EagI
EarI EclXI Eco52I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I KspI MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NdeII NgoMIV NlaIII
NlaIV PflMI PleI PmaCI PmlI
PpuMI PstI PvuII RsaI SacI
SacII Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmaI SmlI StuI
StyI TaqI TfiI TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmaCI XmaI