Restriction map for pFN21AA2010
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# NO CUT ENZYMES (  121 enzymes) #
##################################

Acc65I      AccI        AccII       AclI        AflII       
AgeI        AhdI        Alw44I      AocI        Aor51HI     
ApaI        ApaLI       AscI        Asp718I     AspEI       
AvaI        AviII       AvrII       BalI        BbeI        
BbrPI       BbvCI       BcgI        BciVI       BclI        
BfrI        BglI        BglII       BlnI        BlpI        
Bpu1102I    BsaI        BsiWI       BsmBI       BsmI        
BsoBI       BspDI       BspHI       BspMI       BsrDI       
BssHII      Bst1107I    BstEII      BstPI       BstUI       
BstXI       BstZ17I     Bsu36I      CelII       CfoI        
ClaI        CpoI        DraIII      DrdI        EaeI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRI       FbaI        
FseI        FspI        HaeII       HgaI        HhaI        
HinP1I      KasI        KpnI        KspI        MfeI        
MluI        MluNI       MscI        MspA1I      MunI        
MvnI        NaeI        NarI        NgoMIV      NotI        
NruI        PacI        PaeR7I      PinAI       PmaCI       
PmeI        PmlI        PshAI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        Sse8387I    SwaI        
Tsp45I      XbaI        XcmI        XhoI        XmaCI       
XmaI        


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# ONE CUT ENZYMES (   45 enzymes) #
###################################

AatII         655  GACGTC               GACGT^C
AccIII       1571  TCCGGA               T^CCGGA
AcyI          655  GACGTC               G[GA]^CG[CT]C
AflIII        851  ACATGT               A^C[GA][CT]GT
AseI         1555  ATTAAT               AT^TAAT
AsnI         1555  ATTAAT               AT^TAAT
AspHI        1522  GAGCAC               G[AT]GC[AT]^C
AspI         1065  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BanI           67  GGCACC               G^G[CT][GA]CC
BanII        2181  GAGCCC               G[GA]GC[CT]^C
BsaAI          91  TACGTG               [CT]AC^GT[GA]
BsaBI        1270  GATACAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         655  GACGTC               G[GA]^CG[CT]C
BsaWI        1571  TCCGGA               [AT]^CCGG[AT]
BseAI        1571  TCCGGA               T^CCGGA
BsgI          565  CTGCAC               GTGCAG(16/14)
BsiEI          60  CGACCG               CG[GA][CT]^CG
BsiHKAI      1522  GAGCAC               G[AT]GC[AT]^C
BsmAI        2052  GTCTC                GTCTC(1/5)
BspEI        1571  TCCGGA               T^CCGGA
BspLU11I      851  ACATGT               A^CATGT
BsrBI          97  GAGCGG               CCGCTC(-3/-3)
BsrFI          47  ACCGGC               [GA]^CCGG[CT]
BssSI        2359  CTCGTG               CACGAG(-5/-1)
BstBI        1316  TTCGAA               TT^CGAA
Cfr10I         47  ACCGGC               [GA]^CCGG[CT]
EcoT22I      1405  ATGCAT               ATGCA^T
Hin1I         655  GACGTC               G[GA]^CG[CT]C
HindIII      2194  AAGCTT               A^AGCTT
MaeIII       2427  GTTAC                ^GT[ACGT]AC
MamI         1270  GATACAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI         1571  TCCGGA               T^CCGGA
NcoI         1181  CCATGG               C^CATGG
NdeI         1258  CATATG               CA^TATG
NheI         1344  GCTAGC               G^CTAGC
NsiI         1405  ATGCAT               ATGCA^T
NspV         1316  TTCGAA               TT^CGAA
PflFI        1065  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PleI          157  GAGTC                GAGTC(4/5)
PshBI        1555  ATTAAT               AT^TAAT
SfuI         1316  TTCGAA               TT^CGAA
SphI         1602  GCATGC               GCATG^C
SspI          553  AATATT               AAT^ATT
StuI         2355  AGGCCT               AGG^CCT
Tth111I      1065  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AlwNI         758  CAGATCCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1274  CAGATCCTG           
BbsI         1444  GAAGAC               GAAGAC(2/6)
             2217  GAAGAC              
BbvI         2335  GCAGC                GCAGC(8/12)
             1138  GCTGC               
BpmI         2291  CTCCAG               CTGGAG(16/14)
              520  CTGGAG              
BpuAI        1444  GAAGAC               GAAGAC(2/6)
             2217  GAAGAC              
BseRI         355  GAGGAG               GAGGAG(10/8)
             1289  GAGGAG              
BsmFI          56  GGGAC                GGGAC(10/14)
              114  GTCCC               
Bsp1407I       29  TGTACA               T^GTACA
             1860  TGTACA              
BsrGI          29  TGTACA               T^GTACA
             1860  TGTACA              
BstAPI       1398  GCACTGTATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1615  GCATTATGTGC         
DraII         308  AGGACCC              [GA]G^G[ACGT]CC[CT]
              332  AGGACCT             
DsaI          317  CCGTGG               C^C[GA][CT]GG
             1181  CCATGG              
EcoO109I      308  AGGACCC              [GA]G^G[ACGT]CC[CT]
              332  AGGACCT             
EcoRV        1564  GATATC               GAT^ATC
             1961  GATATC              
EcoT14I      1107  CCTTGG               C^C[AT][AT]GG
             1181  CCATGG              
FauI         1736  CCCGC                CCCGC(4/6)
               18  GCGGG               
HpaI          994  GTTAAC               GTT^AAC
             1506  GTTAAC              
MslI          532  CATGTGTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              571  CACTTGTATG          
PflMI        1299  CCAGCTTATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1479  CCAACTATTGG         
PpuMI         308  AGGACCC              [GA]G^G[AT]CC[CT]
              332  AGGACCT             
SfcI         1033  CTACAG               C^T[GA][CT]AG
             2045  CTGTAG              
SspBI          29  TGTACA               T^GTACA
             1860  TGTACA              
StyI         1107  CCTTGG               C^C[AT][AT]GG
             1181  CCATGG              
TseI         1138  GCTGC                G^C[AT]GC
             2335  GCAGC               
Van91I       1299  CCAGCTTATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1479  CCAACTATTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   67 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        Asp700I     AvaII       BamHI       BcnI        
BfaI        BmyI        BsaJI       BsiYI       BslI        
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
BstYI       Cac8I       Cfr13I      DdeI        DpnII       
DraI        EarI        EcoRII      Fnu4HI      FokI        
HaeIII      HapII       HincII      HindII      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MboI        MboII       MflI        MnlI        
MseI        MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NspI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       TaqI        
TfiI        Tru9I       Tsp509I     TspRI       TthHB8I     
XhoII       XmnI        
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