(Circular) (Six-base) MAPSORT of: pFN21AA2010.seq Check: 2396 from: 1 to: 7165 pFN21AA2010 7165 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2664 4975 278 Size: 53 83 186 936 1128 2311 2468 Fragments arranged by size: 2468 2311 1128 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4508 1937 Size: 2571 4594 Acc65I G'GTAC_C Cuts at: 4486 4486 Size: 7165 AclI AA'CG_TT Cuts at: 1563 5292 5665 1563 Size: 3729 373 3063 Fragments arranged by size: 3729 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2129 2244 2376 2664 5228 6612 6880 1632 Size: 497 115 132 288 2564 1384 268 1917 Fragments arranged by size: 2564 1917 1384 497 288 268 132 115 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6968 AflIII A'CryG_T Cuts at: 1203 2856 6064 7041 1203 Size: 1653 3208 977 1327 Fragments arranged by size: 3208 1653 1327 977 AgeI A'CCGG_T Cuts at: 5996 5996 Size: 7165 AhdI GACnn_n'nnGTC Cuts at: 5894 5894 Size: 7165 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7165 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2138 1184 Size: 954 6211 AlwNI CAG_nnn'CTG Cuts at: 2768 3284 4908 6480 2768 Size: 516 1624 1572 3453 Fragments arranged by size: 3453 1624 1572 516 ApaLI G'TGCA_C Cuts at: 5222 6378 5222 Size: 1156 6009 ApoI r'AATT_y Cuts at: 1493 2280 2717 2784 2930 3014 3380 3782 4109 Size: 787 437 67 146 84 366 402 327 Cuts at: 4109 4454 4474 4700 4852 6961 1493 Size: 345 20 226 152 2109 1697 Fragments arranged by size: 2109 1697 787 437 402 366 345 327 226 152 146 84 67 20 AseI AT'TA_AT Cuts at: 160 1032 3561 5719 160 Size: 872 2529 2158 1606 Fragments arranged by size: 2529 2158 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7165 AvaI C'yCGr_G Cuts at: 1870 1943 4490 1870 Size: 73 2547 4545 Fragments arranged by size: 4545 2547 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3117 3150 3182 3215 3117 Size: 33 32 33 7067 Fragments arranged by size: 7067 33 33 32 BamHI G'GATC_C Cuts at: 3737 4096 4283 4310 4495 4862 3737 Size: 359 187 27 185 367 6040 Fragments arranged by size: 6040 367 359 187 185 27 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2072 4486 5941 618 Size: 325 217 627 285 2414 1455 1842 Fragments arranged by size: 2414 1842 1455 627 325 285 217 BanII G_rGCy'C Cuts at: 1391 4190 4484 1391 Size: 2799 294 4072 Fragments arranged by size: 4072 2799 294 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3456 4229 928 Size: 971 1557 773 3864 Fragments arranged by size: 3864 1557 971 773 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5356 5390 1903 Size: 34 3419 34 3678 Fragments arranged by size: 3678 3419 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5057 6267 5057 Size: 1210 5955 BclI T'GATC_A Cuts at: 1548 1548 Size: 7165 BfrBI ATG'CAT Cuts at: 3412 3412 Size: 7165 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5776 136 Size: 107 122 71 631 605 4104 1525 Fragments arranged by size: 4104 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7160 7160 Size: 7165 BlpI GC'TnA_GC Cuts at: 4582 4582 Size: 7165 Bme1580I G_kGCm'C Cuts at: 1790 2075 3631 5226 6382 1790 Size: 285 1556 1595 1156 2573 Fragments arranged by size: 2573 1595 1556 1156 285 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7165 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4896 5854 442 Size: 416 53 83 399 24 3479 958 1753 Fragments arranged by size: 3479 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3353 1056 Size: 2297 4868 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2546 4281 5825 1239 Size: 1307 1735 1544 2579 Fragments arranged by size: 2579 1735 1544 1307 Bpu10I CC'TnA_GC Cuts at: 2249 2769 2249 Size: 520 6645 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5290 6155 6453 5290 Size: 865 298 6002 Fragments arranged by size: 6002 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5828 882 Size: 617 4329 2219 Fragments arranged by size: 4329 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2098 4956 493 Size: 711 894 2858 2702 Fragments arranged by size: 2858 2702 894 711 BsaBI GATnn'nnATC Cuts at: 3279 7159 3279 Size: 3880 3285 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2661 4972 5354 5989 Size: 53 83 186 936 1128 2311 382 635 Cuts at: 5989 275 Size: 1451 Fragments arranged by size: 2311 1451 1128 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3576 5598 5996 6270 6417 6828 3576 Size: 2022 398 274 147 411 3913 Fragments arranged by size: 3913 2022 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3285 3315 4035 4065 4350 Size: 30 165 30 1882 30 720 30 285 Cuts at: 4350 4380 1178 Size: 30 3963 Fragments arranged by size: 3963 1882 720 285 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2375 3309 1186 Size: 1189 934 5042 Fragments arranged by size: 5042 1189 934 BseYI C'CCAG_C Cuts at: 6368 6864 6368 Size: 496 6669 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2555 1163 Size: 573 819 5773 Fragments arranged by size: 5773 819 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2068 4615 5376 6404 1540 Size: 275 186 67 2547 761 1028 2301 Fragments arranged by size: 2547 2301 1028 761 275 186 67 BsiHKAI G_wGCw'C Cuts at: 3531 5226 5311 6382 3531 Size: 1695 85 1071 4314 Fragments arranged by size: 4314 1695 1071 85 BsmI GAATG_Cn' Cuts at: 4676 4769 4676 Size: 93 7072 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2075 3531 3631 4190 4484 5226 5311 Size: 399 285 1456 100 559 294 742 85 Cuts at: 5311 6382 1391 Size: 1071 2174 Fragments arranged by size: 2174 1456 1071 742 559 399 294 285 100 85 BspEI T'CCGG_A Cuts at: 3576 6828 3576 Size: 3252 3913 BspHI T'CATG_A Cuts at: 5054 6784 5054 Size: 1730 5435 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4520 844 Size: 3676 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2104 5052 1394 Size: 429 281 2948 3507 Fragments arranged by size: 3507 2948 429 281 BsrDI GCAATG_nn' Cuts at: 65 1248 5660 5834 65 Size: 1183 4412 174 1396 Fragments arranged by size: 4412 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2052 5809 5996 1700 Size: 110 242 3757 187 2869 Fragments arranged by size: 3757 2869 242 187 110 BsrGI T'GTAC_A Cuts at: 96 2034 3865 96 Size: 1938 1831 3396 Fragments arranged by size: 3396 1938 1831 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7165 BssSI C'ACGA_G Cuts at: 4364 5225 6237 4364 Size: 861 1012 5292 Fragments arranged by size: 5292 1012 861 BstAPI GCAn_nnn'nTGC Cuts at: 794 3409 3626 794 Size: 2615 217 4333 Fragments arranged by size: 4333 2615 217 BstBI TT'CG_AA Cuts at: 3322 5969 3322 Size: 2647 4518 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7165 BstYI r'GATC_y Cuts at: 1972 2764 3280 3737 4096 4283 4292 4310 4495 Size: 792 516 457 359 187 9 18 185 Cuts at: 4495 4862 5247 5264 6716 6802 6814 7160 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 792 516 457 385 367 359 346 187 185 86 18 17 12 9 BtgI C'CryG_G Cuts at: 513 1065 2322 3186 513 Size: 552 1257 864 4492 Fragments arranged by size: 4492 1257 864 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2367 487 Size: 683 56 1141 5285 Fragments arranged by size: 5285 1141 683 56 BtsI GCAGTG_nn' Cuts at: 2063 2181 3354 3420 4681 5475 5495 2063 Size: 118 1173 66 1261 794 20 3733 Fragments arranged by size: 3733 1261 1173 794 118 66 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7165 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7130 DraI TTT'AAA Cuts at: 2743 3009 3640 3877 4469 2743 Size: 266 631 237 592 5439 Fragments arranged by size: 5439 631 592 266 237 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6172 809 Size: 507 4856 1802 Fragments arranged by size: 4856 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4612 5501 7130 Size: 54 1425 50 275 18 2782 889 1629 Cuts at: 7130 8 Size: 43 Fragments arranged by size: 2782 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4612 1537 Size: 275 2800 4090 Fragments arranged by size: 4090 2800 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2352 2472 3967 3996 5095 1353 Size: 999 120 1495 29 1099 3423 Fragments arranged by size: 3423 1495 1099 999 120 29 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5752 6126 6272 387 Size: 1064 138 4163 374 146 1280 Fragments arranged by size: 4163 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2129 2244 2376 2546 2664 4281 5228 Size: 393 497 115 132 170 118 1617 947 Cuts at: 5228 5825 6612 6880 1239 Size: 597 787 268 1524 Fragments arranged by size: 1617 1524 947 787 597 497 393 268 170 132 118 115 EcoO109I rG'GnC_Cy Cuts at: 2314 2338 2314 Size: 24 7141 EcoRI G'AATT_C Cuts at: 4474 4852 4474 Size: 378 6787 EcoRV GAT'ATC Cuts at: 3571 3968 3571 Size: 397 6768 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3128 3128 Size: 7165 FspI TGC'GCA Cuts at: 5671 5671 Size: 7165 HaeII r_GCGC'y Cuts at: 1678 6312 1678 Size: 4634 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2363 2366 2395 2398 3143 Size: 32 135 32 791 3 29 3 745 Cuts at: 3143 3175 3186 3218 3285 3317 3791 3823 5806 Size: 32 11 32 67 32 474 32 1983 Cuts at: 5806 5838 5880 5912 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1983 791 745 474 135 67 42 32 32 32 32 32 32 32 32 29 11 3 3 HincII GTy'rAC Cuts at: 669 1657 1938 3001 3334 3513 4509 4755 669 Size: 988 281 1063 333 179 996 246 3079 Fragments arranged by size: 3079 1063 996 988 333 281 246 179 HindIII A'AGCT_T Cuts at: 748 4199 748 Size: 3451 3714 HpaI GTT'AAC Cuts at: 3001 3513 4755 3001 Size: 512 1242 5411 Fragments arranged by size: 5411 1242 512 KpnI G_GTAC'C Cuts at: 4490 4490 Size: 7165 MfeI C'AATT_G Cuts at: 4764 4764 Size: 7165 MluI A'CGCG_T Cuts at: 7041 7041 Size: 7165 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6279 6463 1305 Size: 4974 184 2007 Fragments arranged by size: 4974 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5343 Fragments arranged by size: 5343 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2541 2580 5123 5482 5641 518 Size: 648 1375 39 2543 359 159 2042 Fragments arranged by size: 2543 2042 1375 648 359 159 39 MspA1I CmG'CkG Cuts at: 1606 4581 5258 6406 6651 1606 Size: 2975 677 1148 245 2120 Fragments arranged by size: 2975 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 3186 513 Size: 552 2121 4492 Fragments arranged by size: 4492 2121 552 NdeI CA'TA_TG Cuts at: 387 3264 387 Size: 2877 4288 NheI G'CTAG_C Cuts at: 1052 3349 1052 Size: 2297 4868 NotI GC'GGCC_GC Cuts at: 4612 4612 Size: 7165 NsiI A_TGCA'T Cuts at: 3414 3414 Size: 7165 NspI r_CATG'y Cuts at: 1132 1229 2084 2119 2860 2914 3349 3416 3611 Size: 97 855 35 741 54 435 67 195 Cuts at: 3611 4523 6068 1132 Size: 912 1545 2229 Fragments arranged by size: 2229 1545 912 855 741 435 195 97 67 54 35 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7165 PciI A'CATG_T Cuts at: 2856 6064 2856 Size: 3208 3957 PflMI CCAn_nnn'nTGG Cuts at: 1108 3310 3490 1108 Size: 2202 180 4783 Fragments arranged by size: 4783 2202 180 PfoI T'CCnGG_A Cuts at: 2523 4270 4306 2523 Size: 1747 36 5382 Fragments arranged by size: 5382 1747 36 PmeI GTTT'AAAC Cuts at: 4469 4469 Size: 7165 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2131 2163 3573 3605 5234 5266 6747 6779 2131 Size: 32 1410 32 1629 32 1481 32 2517 Fragments arranged by size: 2517 1629 1481 1410 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2314 2338 2314 Size: 24 7141 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7165 PsiI TTA'TAA Cuts at: 4735 4735 Size: 7165 PstI C_TGCA'G Cuts at: 830 4517 830 Size: 3687 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7165 SalI G'TCGA_C Cuts at: 1936 4507 1936 Size: 2571 4594 SbfI CC_TGCA'GG Cuts at: 4517 4517 Size: 7165 ScaI AGT'ACT Cuts at: 5413 7125 5413 Size: 1712 5453 SfcI C'TryA_G Cuts at: 826 1045 3038 4050 4513 5648 6329 6520 826 Size: 219 1993 1012 463 1135 681 191 1471 Fragments arranged by size: 1993 1471 1135 1012 681 463 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7165 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7165 SmaI CCC'GGG Cuts at: 1872 4492 1872 Size: 2620 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5269 6170 6432 820 Size: 197 926 3326 901 262 1553 Fragments arranged by size: 3326 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4956 493 Size: 4463 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7165 SphI G_CATG'C Cuts at: 1132 3611 4523 1132 Size: 2479 912 3774 Fragments arranged by size: 3774 2479 912 SspI AAT'ATT Cuts at: 5 52 2560 5089 5 Size: 47 2508 2529 2081 Fragments arranged by size: 2529 2508 2081 47 StuI AGG'CCT Cuts at: 1517 4362 1517 Size: 2845 4320 StyI C'CwwG_G Cuts at: 513 1065 3112 3186 4604 513 Size: 552 2047 74 1418 3074 Fragments arranged by size: 3074 2047 1418 552 74 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2024 2830 5192 5209 5362 5547 5992 Size: 1255 444 806 2362 17 153 185 445 Cuts at: 5992 325 Size: 1498 Fragments arranged by size: 2362 1498 1255 806 445 444 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2034 3865 5411 Size: 274 80 33 51 1443 57 1831 1546 Cuts at: 5411 7123 96 Size: 1712 138 Fragments arranged by size: 1831 1712 1546 1443 274 138 80 57 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2042 2074 4350 4382 7125 7157 2042 Size: 32 2276 32 2743 32 2050 Fragments arranged by size: 2743 2276 2050 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3073 1316 Size: 1757 5408 XbaI T'CTAG_A Cuts at: 4501 4501 Size: 7165 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7165 XmaI C'CCGG_G Cuts at: 1870 4490 1870 Size: 2620 4545 XmnI GAAnn'nnTTC Cuts at: 2751 2931 4155 5294 2751 Size: 180 1224 1139 4622 Fragments arranged by size: 4622 1224 1139 180 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2662 4973 276 Size: 53 83 186 936 1128 2311 2468 Fragments arranged by size: 2468 2311 1128 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI