(Circular) (Six-base) MAPSORT of: pFN21AA2009.seq Check: 9711 from: 1 to: 6412 pFN21AA2009 6412 bp With 182 enzymes: SgfI * February 27, 2009 14:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4222 278 Size: 53 83 186 936 2686 2468 Fragments arranged by size: 2686 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3755 1937 Size: 1818 4594 Acc65I G'GTAC_C Cuts at: 2503 3733 2503 Size: 1230 5182 AclI AA'CG_TT Cuts at: 1563 4539 4912 1563 Size: 2976 373 3063 Fragments arranged by size: 3063 2976 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4475 5859 6127 1632 Size: 2843 1384 268 1917 Fragments arranged by size: 2843 1917 1384 268 AfeI AGC'GCT Cuts at: 2277 2277 Size: 6412 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6215 AflIII A'CryG_T Cuts at: 1203 2577 3471 5311 6288 1203 Size: 1374 894 1840 977 1327 Fragments arranged by size: 1840 1374 1327 977 894 AgeI A'CCGG_T Cuts at: 5243 5243 Size: 6412 AhdI GACnn_n'nnGTC Cuts at: 5141 5141 Size: 6412 AleI CACnn'nnGTG Cuts at: 1166 2744 1166 Size: 1578 4834 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6412 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2600 2632 2600 Size: 32 6380 AlwNI CAG_nnn'CTG Cuts at: 2796 2947 4155 5727 2796 Size: 151 1208 1572 3481 Fragments arranged by size: 3481 1572 1208 151 ApaI G_GGCC'C Cuts at: 2538 3139 2538 Size: 601 5811 ApaLI G'TGCA_C Cuts at: 3287 4469 5625 3287 Size: 1182 1156 4074 Fragments arranged by size: 4074 1182 1156 ApoI r'AATT_y Cuts at: 1493 3721 3947 4099 6208 1493 Size: 2228 226 152 2109 1697 Fragments arranged by size: 2228 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4966 160 Size: 872 3934 1606 Fragments arranged by size: 3934 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6412 AvaI C'yCGr_G Cuts at: 1870 1943 2477 2655 3074 3154 3239 3349 3394 Size: 73 534 178 419 80 85 110 45 Cuts at: 3394 3659 3737 1870 Size: 265 78 4545 Fragments arranged by size: 4545 534 419 265 178 110 85 80 78 73 45 BamHI G'GATC_C Cuts at: 3742 4109 3742 Size: 367 6045 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2156 2455 2503 2543 2738 Size: 325 217 627 369 299 48 40 195 Cuts at: 2738 3121 3248 3308 3595 3733 5188 618 Size: 383 127 60 287 138 1455 1842 Fragments arranged by size: 1842 1455 627 383 369 325 299 287 217 195 138 127 60 48 40 BanII G_rGCy'C Cuts at: 1391 2104 2526 2538 2709 3139 3482 3660 3731 Size: 713 422 12 171 430 343 178 71 Cuts at: 3731 1391 Size: 4072 Fragments arranged by size: 4072 713 430 422 343 178 171 71 12 BbeI G_GCGC'C Cuts at: 2160 2547 3252 2160 Size: 387 705 5320 Fragments arranged by size: 5320 705 387 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2294 3226 928 Size: 971 395 932 4114 Fragments arranged by size: 4114 971 932 395 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3566 3600 4603 4637 1903 Size: 34 50 34 1545 34 1003 34 3678 Fragments arranged by size: 3678 1545 1003 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4304 5514 4304 Size: 1210 5202 BclI T'GATC_A Cuts at: 1548 1548 Size: 6412 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3353 5023 136 Size: 107 122 71 631 605 1681 1670 1525 Fragments arranged by size: 1681 1670 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6407 6407 Size: 6412 BlpI GC'TnA_GC Cuts at: 3829 3829 Size: 6412 Bme1580I G_kGCm'C Cuts at: 1790 2538 3124 3139 3291 3445 3600 3668 4473 Size: 748 586 15 152 154 155 68 805 Cuts at: 4473 5629 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 805 748 586 155 154 152 68 15 BmgBI CAC'GTC Cuts at: 1318 3474 1318 Size: 2156 4256 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2217 2306 3133 Size: 416 53 83 399 24 800 89 827 Cuts at: 3133 4143 5101 442 Size: 1010 958 1753 Fragments arranged by size: 1753 1010 958 827 800 416 399 89 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6412 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2176 2404 2422 2176 Size: 228 18 6166 Fragments arranged by size: 6166 228 18 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2051 2818 2854 3490 5072 1239 Size: 812 767 36 636 1582 2579 Fragments arranged by size: 2579 1582 812 767 636 36 Bpu10I CC'TnA_GC Cuts at: 2978 2978 Size: 6412 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4537 5402 5700 4537 Size: 865 298 5249 Fragments arranged by size: 5249 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2075 5075 882 Size: 617 576 3000 2219 Fragments arranged by size: 3000 2219 617 576 BsaAI yAC'GTr Cuts at: 493 1204 2580 4203 493 Size: 711 1376 1623 2702 Fragments arranged by size: 2702 1623 1376 711 BsaBI GATnn'nnATC Cuts at: 2760 6406 2760 Size: 3646 2766 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2157 2544 2591 3249 Size: 53 83 186 936 624 387 47 658 Cuts at: 3249 4219 4601 5236 275 Size: 970 382 635 1451 Fragments arranged by size: 1451 970 936 658 635 624 387 382 186 83 53 47 BsaWI w'CCGG_w Cuts at: 3175 4845 5243 5517 5664 6075 3175 Size: 1670 398 274 147 411 3512 Fragments arranged by size: 3512 1670 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2111 2141 2600 2630 2852 Size: 30 165 30 708 30 459 30 222 Cuts at: 2852 2882 1178 Size: 30 4708 Fragments arranged by size: 4708 708 459 222 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2339 2675 3214 3417 3472 3475 3490 3493 Size: 1153 336 539 203 55 3 15 3 Cuts at: 3493 1186 Size: 4105 Fragments arranged by size: 4105 1153 539 336 203 55 15 3 3 BseYI C'CCAG_C Cuts at: 2009 2134 2594 2813 2940 3436 5615 6111 2009 Size: 125 460 219 127 496 2179 496 2310 Fragments arranged by size: 2310 2179 496 496 460 219 127 125 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3284 1163 Size: 573 1548 4291 Fragments arranged by size: 4291 1548 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2836 3862 4623 5651 1540 Size: 275 186 835 1026 761 1028 2301 Fragments arranged by size: 2301 1028 1026 835 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2403 2709 3291 3366 4473 4558 5629 2403 Size: 306 582 75 1107 85 1071 3186 Fragments arranged by size: 3186 1107 1071 582 306 85 75 BsmI GAATG_Cn' Cuts at: 3923 4016 3923 Size: 93 6319 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2104 2403 2526 2538 2709 3124 3139 Size: 399 314 299 123 12 171 415 15 Cuts at: 3139 3291 3366 3445 3482 3600 3660 3668 3731 Size: 152 75 79 37 118 60 8 63 Cuts at: 3731 4473 4558 5629 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 415 399 314 299 171 152 123 118 85 79 75 63 60 37 15 12 8 BspEI T'CCGG_A Cuts at: 6075 6075 Size: 6412 BspHI T'CATG_A Cuts at: 4301 6031 4301 Size: 1730 4682 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2481 3175 3767 844 Size: 1637 694 592 3489 Fragments arranged by size: 3489 1637 694 592 BsrBI CCG'CTC Cuts at: 1394 1823 3107 3148 4299 1394 Size: 429 1284 41 1151 3507 Fragments arranged by size: 3507 1284 1151 429 41 BsrDI GCAATG_nn' Cuts at: 65 1248 2676 3022 4907 5081 65 Size: 1183 1428 346 1885 174 1396 Fragments arranged by size: 1885 1428 1396 1183 346 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2611 2627 2690 3294 5056 5243 1700 Size: 110 801 16 63 604 1762 187 2869 Fragments arranged by size: 2869 1762 801 604 187 110 63 16 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6412 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6412 BssSI C'ACGA_G Cuts at: 2525 4472 5484 2525 Size: 1947 1012 3453 Fragments arranged by size: 3453 1947 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3315 3627 794 Size: 2521 312 3579 Fragments arranged by size: 3579 2521 312 BstBI TT'CG_AA Cuts at: 5216 5216 Size: 6412 BstEII G'GTnAC_C Cuts at: 1184 2623 1184 Size: 1439 4973 BstXI CCAn_nnnn'nTGG Cuts at: 3132 3436 3132 Size: 304 6108 BstYI r'GATC_y Cuts at: 1972 3742 4109 4494 4511 5963 6049 6061 6407 Size: 1770 367 385 17 1452 86 12 346 Cuts at: 6407 1972 Size: 1977 Fragments arranged by size: 1977 1770 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2367 3228 3681 513 Size: 552 1302 861 453 3244 Fragments arranged by size: 3244 1302 861 552 453 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5673 Fragments arranged by size: 5673 683 56 BtsI GCAGTG_nn' Cuts at: 2890 3928 4722 4742 2890 Size: 1038 794 20 4560 Fragments arranged by size: 4560 1038 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6412 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6377 DraI TTT'AAA Cuts at: 3716 3716 Size: 6412 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5419 809 Size: 507 4103 1802 Fragments arranged by size: 4103 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2364 2833 3604 Size: 54 1425 50 275 18 534 469 771 Cuts at: 3604 3859 4748 6377 8 Size: 255 889 1629 43 Fragments arranged by size: 1629 1425 889 771 534 469 275 255 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2833 3859 1537 Size: 275 1021 1026 4090 Fragments arranged by size: 4090 1026 1021 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2183 3199 3493 3517 3526 3620 3644 4342 Size: 830 1016 294 24 9 94 24 698 Cuts at: 4342 1353 Size: 3423 Fragments arranged by size: 3423 1016 830 698 294 94 24 24 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2966 3177 4999 5373 5519 387 Size: 1064 138 1377 211 1822 374 146 1280 Fragments arranged by size: 1822 1377 1280 1064 374 211 146 138 EcoICRI GAG'CTC Cuts at: 2707 2707 Size: 6412 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2051 2818 2854 3490 4475 5072 5859 Size: 393 419 767 36 636 985 597 787 Cuts at: 5859 6127 1239 Size: 268 1524 Fragments arranged by size: 1524 985 787 767 636 597 419 393 268 36 EcoO109I rG'GnC_Cy Cuts at: 2534 2534 Size: 6412 EcoRI G'AATT_C Cuts at: 3721 4099 3721 Size: 378 6034 FspI TGC'GCA Cuts at: 2988 3386 4918 2988 Size: 398 1532 4482 Fragments arranged by size: 4482 1532 398 HaeII r_GCGC'y Cuts at: 1678 2160 2279 2547 2947 3252 5559 1678 Size: 482 119 268 400 305 2307 2531 Fragments arranged by size: 2531 2307 482 400 305 268 119 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2111 2143 5053 5085 5127 Size: 32 135 32 539 32 2910 32 42 Cuts at: 5127 5159 1373 Size: 32 2626 Fragments arranged by size: 2910 2626 539 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3756 4002 669 Size: 988 281 1818 246 3079 Fragments arranged by size: 3079 1818 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6412 HpaI GTT'AAC Cuts at: 4002 4002 Size: 6412 KasI G'GCGC_C Cuts at: 2156 2543 3248 2156 Size: 387 705 5320 Fragments arranged by size: 5320 705 387 KpnI G_GTAC'C Cuts at: 2507 3737 2507 Size: 1230 5182 MfeI C'AATT_G Cuts at: 4011 4011 Size: 6412 MluI A'CGCG_T Cuts at: 6288 6288 Size: 6412 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3288 3447 5526 5710 1305 Size: 1983 159 2079 184 2007 Fragments arranged by size: 2079 2007 1983 184 159 MscI TGG'CCA Cuts at: 10 64 1489 1832 2366 3606 10 Size: 54 1425 343 534 1240 2816 Fragments arranged by size: 2816 1425 1240 534 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2495 2744 4370 4729 4888 518 Size: 648 1329 249 1626 359 159 2042 Fragments arranged by size: 2042 1626 1329 648 359 249 159 MspA1I CmG'CkG Cuts at: 1606 2046 2070 2933 3035 3315 3448 3683 3828 Size: 440 24 863 102 280 133 235 145 Cuts at: 3828 4505 5653 5898 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 863 677 440 280 245 235 145 133 102 24 NaeI GCC'GGC Cuts at: 3296 3296 Size: 6412 NarI GG'CG_CC Cuts at: 2157 2544 3249 2157 Size: 387 705 5320 Fragments arranged by size: 5320 705 387 NcoI C'CATG_G Cuts at: 513 1065 2367 513 Size: 552 1302 4558 Fragments arranged by size: 4558 1302 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6412 NgoMIV G'CCGG_C Cuts at: 3294 3294 Size: 6412 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6412 NotI GC'GGCC_GC Cuts at: 3859 3859 Size: 6412 NspI r_CATG'y Cuts at: 1132 1229 2237 2635 3770 5315 1132 Size: 97 1008 398 1135 1545 2229 Fragments arranged by size: 2229 1545 1135 1008 398 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6412 PciI A'CATG_T Cuts at: 5311 5311 Size: 6412 PflMI CCAn_nnn'nTGG Cuts at: 1108 2947 1108 Size: 1839 4573 PfoI T'CCnGG_A Cuts at: 2423 2423 Size: 6412 PmeI GTTT'AAAC Cuts at: 3716 3716 Size: 6412 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2600 2632 4481 4513 5994 6026 2600 Size: 32 1849 32 1481 32 2986 Fragments arranged by size: 2986 1849 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6412 PsiI TTA'TAA Cuts at: 3982 3982 Size: 6412 PspOMI G'GGCC_C Cuts at: 2534 3135 2534 Size: 601 5811 PstI C_TGCA'G Cuts at: 830 2109 3084 3172 3764 830 Size: 1279 975 88 592 3478 Fragments arranged by size: 3478 1279 975 592 88 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6412 PvuII CAG'CTG Cuts at: 3315 3448 3315 Size: 133 6279 SacI G_AGCT'C Cuts at: 2709 2709 Size: 6412 SacII CC_GC'GG Cuts at: 3684 3684 Size: 6412 SalI G'TCGA_C Cuts at: 1936 3754 1936 Size: 1818 4594 SapI GCTCTTCn'nnn_ Cuts at: 2183 3199 3517 3644 2183 Size: 1016 318 127 4951 Fragments arranged by size: 4951 1016 318 127 SbfI CC_TGCA'GG Cuts at: 3764 3764 Size: 6412 ScaI AGT'ACT Cuts at: 4660 6372 4660 Size: 1712 4700 SfcI C'TryA_G Cuts at: 826 1045 2105 2956 3080 3168 3760 4895 5576 Size: 219 1060 851 124 88 592 1135 681 Cuts at: 5576 5767 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1060 851 681 592 219 191 124 88 SfoI GGC'GCC Cuts at: 2158 2545 3250 2158 Size: 387 705 5320 Fragments arranged by size: 5320 705 387 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6412 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6412 SmaI CCC'GGG Cuts at: 1872 2479 3156 3241 3351 3396 3739 1872 Size: 607 677 85 110 45 343 4545 Fragments arranged by size: 4545 677 607 343 110 85 45 SmlI C'TyrA_G Cuts at: 820 1017 1943 2655 4516 5417 5679 820 Size: 197 926 712 1861 901 262 1553 Fragments arranged by size: 1861 1553 926 901 712 262 197 SnaBI TAC'GTA Cuts at: 493 4203 493 Size: 3710 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6412 SphI G_CATG'C Cuts at: 1132 2635 3770 1132 Size: 1503 1135 3774 Fragments arranged by size: 3774 1503 1135 SrfI GCCC'GGGC Cuts at: 2479 2479 Size: 6412 SspI AAT'ATT Cuts at: 5 52 4336 5 Size: 47 4284 2081 Fragments arranged by size: 4284 2081 47 StuI AGG'CCT Cuts at: 1517 3408 1517 Size: 1891 4521 StyI C'CwwG_G Cuts at: 513 1065 2367 3851 513 Size: 552 1302 1484 3074 Fragments arranged by size: 3074 1484 1302 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2219 4439 4456 4609 4794 5239 325 Size: 1255 639 2220 17 153 185 445 1498 Fragments arranged by size: 2220 1498 1255 639 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4658 6370 96 Size: 274 80 33 51 1443 2681 1712 138 Fragments arranged by size: 2681 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6372 6404 6372 Size: 32 6380 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6412 XbaI T'CTAG_A Cuts at: 3748 3748 Size: 6412 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2793 3271 2793 Size: 478 5934 XhoI C'TCGA_G Cuts at: 1943 2655 1943 Size: 712 5700 XmaI C'CCGG_G Cuts at: 1870 2477 3154 3239 3349 3394 3737 1870 Size: 607 677 85 110 45 343 4545 Fragments arranged by size: 4545 677 607 343 110 85 45 XmnI GAAnn'nnTTC Cuts at: 2723 4541 2723 Size: 1818 4594 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4220 276 Size: 53 83 186 936 2686 2468 Fragments arranged by size: 2686 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SanDI SexAI SfiI SwaI