(Circular) (Six-base) MAPSORT of: pFN21AA1966.seq Check: 2173 from: 1 to: 6886 pFN21AA1966 6886 bp With 182 enzymes: SgfI * February 27, 2009 14:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3571 4696 278 Size: 53 83 186 936 2035 1125 2468 Fragments arranged by size: 2468 2035 1125 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3610 4033 4229 1937 Size: 1673 423 196 4594 Fragments arranged by size: 4594 1673 423 196 Acc65I G'GTAC_C Cuts at: 4207 4207 Size: 6886 AclI AA'CG_TT Cuts at: 1563 5013 5386 1563 Size: 3450 373 3063 Fragments arranged by size: 3450 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2088 2553 3237 4949 6333 6601 1632 Size: 456 465 684 1712 1384 268 1917 Fragments arranged by size: 1917 1712 1384 684 465 456 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6689 AflIII A'CryG_T Cuts at: 1203 5785 6762 1203 Size: 4582 977 1327 Fragments arranged by size: 4582 1327 977 AgeI A'CCGG_T Cuts at: 5717 5717 Size: 6886 AhdI GACnn_n'nnGTC Cuts at: 5615 5615 Size: 6886 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6886 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6886 AlwNI CAG_nnn'CTG Cuts at: 4629 6201 4629 Size: 1572 5314 ApaLI G'TGCA_C Cuts at: 4943 6099 4943 Size: 1156 5730 ApoI r'AATT_y Cuts at: 1493 2346 3227 3788 4195 4421 4573 6682 1493 Size: 853 881 561 407 226 152 2109 1697 Fragments arranged by size: 2109 1697 881 853 561 407 226 152 AseI AT'TA_AT Cuts at: 160 1032 5440 160 Size: 872 4408 1606 Fragments arranged by size: 4408 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6886 AvaI C'yCGr_G Cuts at: 1870 1943 4211 1870 Size: 73 2268 4545 Fragments arranged by size: 4545 2268 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2055 2088 2055 Size: 33 6853 BamHI G'GATC_C Cuts at: 2419 3700 4216 4583 2419 Size: 1281 516 367 4722 Fragments arranged by size: 4722 1281 516 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4207 5662 618 Size: 325 217 627 2420 1455 1842 Fragments arranged by size: 2420 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2231 4205 1391 Size: 840 1974 4072 Fragments arranged by size: 4072 1974 840 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2502 3464 928 Size: 971 603 962 4350 Fragments arranged by size: 4350 971 962 603 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5077 5111 1903 Size: 34 3140 34 3678 Fragments arranged by size: 3678 3140 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4778 5988 4778 Size: 1210 5676 BclI T'GATC_A Cuts at: 1548 1548 Size: 6886 BfrBI ATG'CAT Cuts at: 3544 3544 Size: 6886 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 5497 136 Size: 107 122 71 631 605 337 3488 1525 Fragments arranged by size: 3488 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 2309 2861 3179 6881 2309 Size: 552 318 3702 2314 Fragments arranged by size: 3702 2314 552 318 BlpI GC'TnA_GC Cuts at: 4303 4303 Size: 6886 Bme1580I G_kGCm'C Cuts at: 1790 4947 6103 1790 Size: 3157 1156 2573 Fragments arranged by size: 3157 2573 1156 BmgBI CAC'GTC Cuts at: 1318 2489 3428 4112 1318 Size: 1171 939 684 4092 Fragments arranged by size: 4092 1171 939 684 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3295 4617 5575 Size: 416 53 83 399 24 1878 1322 958 Cuts at: 5575 442 Size: 1753 Fragments arranged by size: 1878 1753 1322 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6886 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2353 5546 1239 Size: 1114 3193 2579 Fragments arranged by size: 3193 2579 1114 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3879 5011 5876 6174 3879 Size: 1132 865 298 4591 Fragments arranged by size: 4591 1132 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2763 3676 4041 4059 4149 5549 882 Size: 617 1264 913 365 18 90 1400 2219 Fragments arranged by size: 2219 1400 1264 913 617 365 90 18 BsaAI yAC'GTr Cuts at: 493 1204 4677 493 Size: 711 3473 2702 Fragments arranged by size: 3473 2702 711 BsaBI GATnn'nnATC Cuts at: 2337 6880 2337 Size: 4543 2343 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3568 3607 4693 5075 Size: 53 83 186 936 2035 39 1086 382 Cuts at: 5075 5710 275 Size: 635 1451 Fragments arranged by size: 2035 1451 1086 936 635 382 186 83 53 39 BsaWI w'CCGG_w Cuts at: 2409 5319 5717 5991 6138 6549 2409 Size: 2910 398 274 147 411 2746 Fragments arranged by size: 2910 2746 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6661 Fragments arranged by size: 6661 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2039 2621 2708 2711 2714 2723 2726 2729 Size: 853 582 87 3 3 9 3 3 Cuts at: 2729 2738 2741 2744 2747 2750 2795 3882 1186 Size: 9 3 3 3 3 45 1087 4190 Fragments arranged by size: 4190 1087 853 582 87 45 9 9 3 3 3 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 3319 3462 6089 6585 3319 Size: 143 2627 496 3620 Fragments arranged by size: 3620 2627 496 143 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6313 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4336 5097 6125 1540 Size: 275 186 2335 761 1028 2301 Fragments arranged by size: 2335 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2231 4947 5032 6103 2231 Size: 2716 85 1071 3014 Fragments arranged by size: 3014 2716 1071 85 BsmI GAATG_Cn' Cuts at: 3544 3546 3831 4397 4490 3544 Size: 2 285 566 93 5940 Fragments arranged by size: 5940 566 285 93 2 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2231 4205 4947 5032 6103 1391 Size: 399 441 1974 742 85 1071 2174 Fragments arranged by size: 2174 1974 1071 742 441 399 85 BspEI T'CCGG_A Cuts at: 2409 6549 2409 Size: 4140 2746 BspHI T'CATG_A Cuts at: 4775 6505 4775 Size: 1730 5156 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2966 4241 844 Size: 2122 1275 3489 Fragments arranged by size: 3489 2122 1275 BsrBI CCG'CTC Cuts at: 1394 1823 4773 1394 Size: 429 2950 3507 Fragments arranged by size: 3507 2950 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5381 5555 65 Size: 1183 4133 174 1396 Fragments arranged by size: 4133 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5530 5717 1700 Size: 110 3720 187 2869 Fragments arranged by size: 3720 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6886 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6886 BssSI C'ACGA_G Cuts at: 3561 4946 5958 3561 Size: 1385 1012 4489 Fragments arranged by size: 4489 1385 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3826 794 Size: 3032 3854 BstBI TT'CG_AA Cuts at: 5690 5690 Size: 6886 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6886 BstXI CCAn_nnnn'nTGG Cuts at: 2558 3816 2558 Size: 1258 5628 BstYI r'GATC_y Cuts at: 1972 2309 2419 2498 2861 3179 3272 3700 4216 Size: 337 110 79 363 318 93 428 516 Cuts at: 4216 4583 4968 4985 6437 6523 6535 6881 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 516 428 385 367 363 346 337 318 110 93 86 79 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5395 Fragments arranged by size: 5395 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6147 Fragments arranged by size: 6147 683 56 BtsI GCAGTG_nn' Cuts at: 2572 4402 5196 5216 2572 Size: 1830 794 20 4242 Fragments arranged by size: 4242 1830 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6886 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6851 DraI TTT'AAA Cuts at: 3334 3696 3759 4190 3334 Size: 362 63 431 6030 Fragments arranged by size: 6030 431 362 63 DraIII CAC_nnn'GTG Cuts at: 3289 3289 Size: 6886 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5893 809 Size: 507 4577 1802 Fragments arranged by size: 4577 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4021 4333 5222 Size: 54 1425 50 275 18 2191 312 889 Cuts at: 5222 6851 8 Size: 1629 43 Fragments arranged by size: 2191 1629 1425 889 312 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4333 1537 Size: 275 2521 4090 Fragments arranged by size: 4090 2521 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2667 2700 2715 2903 4177 4816 1353 Size: 1314 33 15 188 1274 639 3423 Fragments arranged by size: 3423 1314 1274 639 188 33 15 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5473 5847 5993 387 Size: 1064 138 3884 374 146 1280 Fragments arranged by size: 3884 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2229 2229 Size: 6886 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2088 2353 2553 3237 4949 5546 6333 Size: 393 456 265 200 684 1712 597 787 Cuts at: 6333 6601 1239 Size: 268 1524 Fragments arranged by size: 1712 1524 787 684 597 456 393 268 265 200 EcoNI CCTnn'n_nnAGG Cuts at: 3819 3837 3912 3819 Size: 18 75 6793 Fragments arranged by size: 6793 75 18 EcoO109I rG'GnC_Cy Cuts at: 3765 3765 Size: 6886 EcoRI G'AATT_C Cuts at: 4195 4573 4195 Size: 378 6508 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3267 3267 Size: 6886 FspI TGC'GCA Cuts at: 5392 5392 Size: 6886 HaeII r_GCGC'y Cuts at: 1678 6033 1678 Size: 4355 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2987 3019 3063 3092 3095 Size: 32 135 32 1415 32 44 29 3 Cuts at: 3095 3124 3573 3605 3646 3678 5527 5559 5601 Size: 29 449 32 41 32 1849 32 42 Cuts at: 5601 5633 1373 Size: 32 2626 Fragments arranged by size: 2626 1849 1415 449 135 44 42 41 32 32 32 32 32 32 32 29 29 3 HincII GTy'rAC Cuts at: 669 1657 1938 3611 4230 4476 669 Size: 988 281 1673 619 246 3079 Fragments arranged by size: 3079 1673 988 619 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6886 HpaI GTT'AAC Cuts at: 4476 4476 Size: 6886 KpnI G_GTAC'C Cuts at: 4211 4211 Size: 6886 MfeI C'AATT_G Cuts at: 2931 4485 2931 Size: 1554 5332 MluI A'CGCG_T Cuts at: 6762 6762 Size: 6886 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6000 6184 1305 Size: 4695 184 2007 Fragments arranged by size: 4695 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5064 Fragments arranged by size: 5064 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3102 3969 4844 5203 5362 518 Size: 648 1936 867 875 359 159 2042 Fragments arranged by size: 2042 1936 875 867 648 359 159 MspA1I CmG'CkG Cuts at: 1606 4302 4979 6127 6372 1606 Size: 2696 677 1148 245 2120 Fragments arranged by size: 2696 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5395 Fragments arranged by size: 5395 939 552 NdeI CA'TA_TG Cuts at: 387 2971 387 Size: 2584 4302 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6886 NotI GC'GGCC_GC Cuts at: 4333 4333 Size: 6886 NsiI A_TGCA'T Cuts at: 3546 3546 Size: 6886 NspI r_CATG'y Cuts at: 1132 1229 4244 5789 1132 Size: 97 3015 1545 2229 Fragments arranged by size: 3015 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6886 PciI A'CATG_T Cuts at: 5785 5785 Size: 6886 PflMI CCAn_nnn'nTGG Cuts at: 1108 3396 3718 1108 Size: 2288 322 4276 Fragments arranged by size: 4276 2288 322 PfoI T'CCnGG_A Cuts at: 3591 3591 Size: 6886 PmeI GTTT'AAAC Cuts at: 4190 4190 Size: 6886 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4955 4987 6468 6500 4955 Size: 32 1481 32 5341 Fragments arranged by size: 5341 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3765 3765 Size: 6886 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6886 PsiI TTA'TAA Cuts at: 2382 4456 2382 Size: 2074 4812 PstI C_TGCA'G Cuts at: 830 4238 830 Size: 3408 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6886 SacI G_AGCT'C Cuts at: 2231 2231 Size: 6886 SalI G'TCGA_C Cuts at: 1936 3609 4228 1936 Size: 1673 619 4594 Fragments arranged by size: 4594 1673 619 SbfI CC_TGCA'GG Cuts at: 4238 4238 Size: 6886 ScaI AGT'ACT Cuts at: 5134 6846 5134 Size: 1712 5174 SfcI C'TryA_G Cuts at: 826 1045 4234 5369 6050 6241 826 Size: 219 3189 1135 681 191 1471 Fragments arranged by size: 3189 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6886 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6886 SmaI CCC'GGG Cuts at: 1872 4213 1872 Size: 2341 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3894 4990 5891 6153 820 Size: 197 926 1951 1096 901 262 1553 Fragments arranged by size: 1951 1553 1096 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4677 493 Size: 4184 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6886 SphI G_CATG'C Cuts at: 1132 4244 1132 Size: 3112 3774 SspI AAT'ATT Cuts at: 5 52 3204 4810 5 Size: 47 3152 1606 2081 Fragments arranged by size: 3152 2081 1606 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6886 StyI C'CwwG_G Cuts at: 513 1065 2004 3395 3650 3815 4325 513 Size: 552 939 1391 255 165 510 3074 Fragments arranged by size: 3074 1391 939 552 510 255 165 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3277 4173 4913 4930 5083 5268 5713 Size: 1255 1697 896 740 17 153 185 445 Cuts at: 5713 325 Size: 1498 Fragments arranged by size: 1697 1498 1255 896 740 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2261 3961 5132 Size: 274 80 33 51 1443 284 1700 1171 Cuts at: 5132 6844 96 Size: 1712 138 Fragments arranged by size: 1712 1700 1443 1171 284 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6846 6878 6846 Size: 32 6854 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6886 XbaI T'CTAG_A Cuts at: 2329 4222 2329 Size: 1893 4993 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3813 3813 Size: 6886 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6886 XmaI C'CCGG_G Cuts at: 1870 4211 1870 Size: 2341 4545 XmnI GAAnn'nnTTC Cuts at: 3544 5015 3544 Size: 1471 5415 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3569 4694 276 Size: 53 83 186 936 2035 1125 2468 Fragments arranged by size: 2468 2035 1125 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI