(Circular) (Six-base) MAPSORT of: pFN21AA1805.seq Check: 3539 from: 1 to: 6406 pFN21AA1805 6406 bp With 182 enzymes: SgfI * February 27, 2009 14:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4216 278 Size: 53 83 186 936 2680 2468 Fragments arranged by size: 2680 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3045 3252 3749 1937 Size: 1108 207 497 4594 Fragments arranged by size: 4594 1108 497 207 Acc65I G'GTAC_C Cuts at: 3118 3727 3118 Size: 609 5797 AclI AA'CG_TT Cuts at: 1563 4533 4906 1563 Size: 2970 373 3063 Fragments arranged by size: 3063 2970 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3092 3151 4469 5853 6121 1632 Size: 1460 59 1318 1384 268 1917 Fragments arranged by size: 1917 1460 1384 1318 268 59 AflII C'TTAA_G Cuts at: 820 1017 2158 820 Size: 197 1141 5068 Fragments arranged by size: 5068 1141 197 AflIII A'CryG_T Cuts at: 1203 5305 6282 1203 Size: 4102 977 1327 Fragments arranged by size: 4102 1327 977 AgeI A'CCGG_T Cuts at: 5237 5237 Size: 6406 AhdI GACnn_n'nnGTC Cuts at: 5135 5135 Size: 6406 AleI CACnn'nnGTG Cuts at: 1166 2877 1166 Size: 1711 4695 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6406 AlwNI CAG_nnn'CTG Cuts at: 3061 3115 4149 5721 3061 Size: 54 1034 1572 3746 Fragments arranged by size: 3746 1572 1034 54 ApaLI G'TGCA_C Cuts at: 4463 5619 4463 Size: 1156 5250 ApoI r'AATT_y Cuts at: 1493 2348 3347 3715 3941 4093 6202 1493 Size: 855 999 368 226 152 2109 1697 Fragments arranged by size: 2109 1697 999 855 368 226 152 AseI AT'TA_AT Cuts at: 160 1032 4960 160 Size: 872 3928 1606 Fragments arranged by size: 3928 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6406 AvaI C'yCGr_G Cuts at: 1870 1943 3548 3731 1870 Size: 73 1605 183 4545 Fragments arranged by size: 4545 1605 183 73 BamHI G'GATC_C Cuts at: 3736 4103 3736 Size: 367 6039 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3118 3727 5182 618 Size: 325 217 627 1331 609 1455 1842 Fragments arranged by size: 1842 1455 1331 627 609 325 217 BanII G_rGCy'C Cuts at: 1391 3159 3290 3725 1391 Size: 1768 131 435 4072 Fragments arranged by size: 4072 1768 435 131 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2001 2343 2549 3200 3323 928 Size: 971 102 342 206 651 123 4011 Fragments arranged by size: 4011 971 651 342 206 123 102 BbvCI CC'TCA_GC Cuts at: 3396 3396 Size: 6406 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2059 2093 4597 4631 1903 Size: 34 122 34 2504 34 3678 Fragments arranged by size: 3678 2504 122 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4298 5508 4298 Size: 1210 5196 BclI T'GATC_A Cuts at: 1548 1548 Size: 6406 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5017 136 Size: 107 122 71 631 605 3345 1525 Fragments arranged by size: 3345 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6401 6401 Size: 6406 BlpI GC'TnA_GC Cuts at: 3823 3823 Size: 6406 Bme1580I G_kGCm'C Cuts at: 1790 4467 5623 1790 Size: 2677 1156 2573 Fragments arranged by size: 2677 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6406 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2098 2756 4137 Size: 416 53 83 399 24 681 658 1381 Cuts at: 4137 5095 442 Size: 958 1753 Fragments arranged by size: 1753 1381 958 681 658 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6406 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2794 3082 2794 Size: 288 6118 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2764 5066 1239 Size: 1525 2302 2579 Fragments arranged by size: 2579 2302 1525 Bpu10I CC'TnA_GC Cuts at: 2756 3396 2756 Size: 640 5766 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4531 5396 5694 4531 Size: 865 298 5243 Fragments arranged by size: 5243 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3516 5069 882 Size: 617 2017 1553 2219 Fragments arranged by size: 2219 2017 1553 617 BsaAI yAC'GTr Cuts at: 493 1204 4197 493 Size: 711 2993 2702 Fragments arranged by size: 2993 2702 711 BsaBI GATnn'nnATC Cuts at: 6400 6400 Size: 6406 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4213 4595 5230 275 Size: 53 83 186 936 2680 382 635 1451 Fragments arranged by size: 2680 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4839 5237 5511 5658 6069 4839 Size: 398 274 147 411 5176 Fragments arranged by size: 5176 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2465 2495 3368 3398 1178 Size: 30 165 30 1062 30 873 30 4186 Fragments arranged by size: 4186 1062 873 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2080 2486 3601 1186 Size: 894 406 1115 3991 Fragments arranged by size: 3991 1115 894 406 BseYI C'CCAG_C Cuts at: 5609 6105 5609 Size: 496 5910 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5833 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3856 4617 5645 1540 Size: 275 186 1855 761 1028 2301 Fragments arranged by size: 2301 1855 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2072 2111 4467 4552 5623 2072 Size: 39 2356 85 1071 2855 Fragments arranged by size: 2855 2356 1071 85 39 BsmI GAATG_Cn' Cuts at: 3917 4010 3917 Size: 93 6313 BsmBI CGTCTCn'nnnn_ Cuts at: 3016 3016 Size: 6406 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2072 2111 3159 3290 3725 4467 4552 Size: 399 282 39 1048 131 435 742 85 Cuts at: 4552 5623 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 1048 742 435 399 282 131 85 39 BspEI T'CCGG_A Cuts at: 6069 6069 Size: 6406 BspHI T'CATG_A Cuts at: 2146 4295 6025 2146 Size: 2149 1730 2527 Fragments arranged by size: 2527 2149 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2531 3761 844 Size: 1687 1230 3489 Fragments arranged by size: 3489 1687 1230 BsrBI CCG'CTC Cuts at: 1394 1823 4293 1394 Size: 429 2470 3507 Fragments arranged by size: 3507 2470 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2483 4901 5075 65 Size: 1183 1235 2418 174 1396 Fragments arranged by size: 2418 1396 1235 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5050 5237 1700 Size: 110 3240 187 2869 Fragments arranged by size: 3240 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3293 96 Size: 3197 3209 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6406 BssSI C'ACGA_G Cuts at: 2071 4466 5478 2071 Size: 2395 1012 2999 Fragments arranged by size: 2999 2395 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6406 BstBI TT'CG_AA Cuts at: 5210 5210 Size: 6406 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6406 BstXI CCAn_nnnn'nTGG Cuts at: 2800 2800 Size: 6406 BstYI r'GATC_y Cuts at: 1972 3736 4103 4488 4505 5957 6043 6055 6401 Size: 1764 367 385 17 1452 86 12 346 Cuts at: 6401 1972 Size: 1977 Fragments arranged by size: 1977 1764 1452 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3046 3046 Size: 6406 Bsu36I CC'TnA_GG Cuts at: 2921 2921 Size: 6406 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5854 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5667 Fragments arranged by size: 5667 683 56 BtsI GCAGTG_nn' Cuts at: 2512 2538 3265 3922 4716 4736 2512 Size: 26 727 657 794 20 4182 Fragments arranged by size: 4182 794 727 657 26 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6406 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6371 DraI TTT'AAA Cuts at: 2559 3710 2559 Size: 1151 5255 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5413 809 Size: 507 4097 1802 Fragments arranged by size: 4097 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3078 3853 4742 Size: 54 1425 50 275 18 1248 775 889 Cuts at: 4742 6371 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1248 889 775 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3853 1537 Size: 275 2041 4090 Fragments arranged by size: 4090 2041 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4336 1353 Size: 2983 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4993 5367 5513 387 Size: 1064 138 3404 374 146 1280 Fragments arranged by size: 3404 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2764 3092 3151 4469 5066 5853 6121 Size: 393 1132 328 59 1318 597 787 268 Cuts at: 6121 1239 Size: 1524 Fragments arranged by size: 1524 1318 1132 787 597 393 328 268 59 EcoO109I rG'GnC_Cy Cuts at: 2042 2100 3112 2042 Size: 58 1012 5336 Fragments arranged by size: 5336 1012 58 EcoRI G'AATT_C Cuts at: 3715 4093 3715 Size: 378 6028 EcoRV GAT'ATC Cuts at: 2807 3202 2807 Size: 395 6011 FspI TGC'GCA Cuts at: 4912 4912 Size: 6406 HaeII r_GCGC'y Cuts at: 1678 5553 1678 Size: 3875 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2629 2661 5047 5079 5121 Size: 32 135 32 1057 32 2386 32 42 Cuts at: 5121 5153 1373 Size: 32 2626 Fragments arranged by size: 2626 2386 1057 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3750 3996 669 Size: 988 281 1812 246 3079 Fragments arranged by size: 3079 1812 988 281 246 HindIII A'AGCT_T Cuts at: 748 2369 3433 748 Size: 1621 1064 3721 Fragments arranged by size: 3721 1621 1064 HpaI GTT'AAC Cuts at: 3996 3996 Size: 6406 KpnI G_GTAC'C Cuts at: 3122 3731 3122 Size: 609 5797 MfeI C'AATT_G Cuts at: 4005 4005 Size: 6406 MluI A'CGCG_T Cuts at: 6282 6282 Size: 6406 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5520 5704 1305 Size: 4215 184 2007 Fragments arranged by size: 4215 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3080 10 Size: 54 1425 343 1248 3336 Fragments arranged by size: 3336 1425 1248 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2182 2607 2877 4364 4723 4882 518 Size: 648 1016 425 270 1487 359 159 2042 Fragments arranged by size: 2042 1487 1016 648 425 359 270 159 MspA1I CmG'CkG Cuts at: 1606 2261 3463 3822 4499 5647 5892 1606 Size: 655 1202 359 677 1148 245 2120 Fragments arranged by size: 2120 1202 1148 677 655 359 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5854 NdeI CA'TA_TG Cuts at: 387 387 Size: 6406 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6406 NotI GC'GGCC_GC Cuts at: 3853 3853 Size: 6406 NspI r_CATG'y Cuts at: 1132 1229 2767 3764 5309 1132 Size: 97 1538 997 1545 2229 Fragments arranged by size: 2229 1545 1538 997 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6406 PciI A'CATG_T Cuts at: 5305 5305 Size: 6406 PflMI CCAn_nnn'nTGG Cuts at: 1108 2533 3087 1108 Size: 1425 554 4427 Fragments arranged by size: 4427 1425 554 PmeI GTTT'AAAC Cuts at: 3710 3710 Size: 6406 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3389 3421 4475 4507 5988 6020 3389 Size: 32 1054 32 1481 32 3775 Fragments arranged by size: 3775 1481 1054 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2042 2100 3112 2042 Size: 58 1012 5336 Fragments arranged by size: 5336 1012 58 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6406 PsiI TTA'TAA Cuts at: 3976 3976 Size: 6406 PstI C_TGCA'G Cuts at: 830 2845 3758 830 Size: 2015 913 3478 Fragments arranged by size: 3478 2015 913 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6406 PvuII CAG'CTG Cuts at: 2261 2261 Size: 6406 SalI G'TCGA_C Cuts at: 1936 3748 1936 Size: 1812 4594 SanDI GG'GwC_CC Cuts at: 2042 2042 Size: 6406 SbfI CC_TGCA'GG Cuts at: 3758 3758 Size: 6406 ScaI AGT'ACT Cuts at: 3180 4654 6366 3180 Size: 1474 1712 3220 Fragments arranged by size: 3220 1712 1474 SexAI A'CCwGG_T Cuts at: 3114 3114 Size: 6406 SfcI C'TryA_G Cuts at: 826 1045 2841 3053 3253 3754 4889 5570 5761 Size: 219 1796 212 200 501 1135 681 191 Cuts at: 5761 826 Size: 1471 Fragments arranged by size: 1796 1471 1135 681 501 219 212 200 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6406 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6406 SmaI CCC'GGG Cuts at: 1872 3733 1872 Size: 1861 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2158 4510 5411 5673 820 Size: 197 926 215 2352 901 262 1553 Fragments arranged by size: 2352 1553 926 901 262 215 197 SnaBI TAC'GTA Cuts at: 493 4197 493 Size: 3704 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6406 SphI G_CATG'C Cuts at: 1132 2767 3764 1132 Size: 1635 997 3774 Fragments arranged by size: 3774 1635 997 SspI AAT'ATT Cuts at: 5 52 4330 5 Size: 47 4278 2081 Fragments arranged by size: 4278 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6406 StyI C'CwwG_G Cuts at: 513 1065 2301 3081 3845 513 Size: 552 1236 780 764 3074 Fragments arranged by size: 3074 1236 780 764 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4433 4450 4603 4788 5233 325 Size: 1255 2853 17 153 185 445 1498 Fragments arranged by size: 2853 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2133 3178 3293 Size: 274 80 33 51 1443 156 1045 115 Cuts at: 3293 4652 6364 96 Size: 1359 1712 138 Fragments arranged by size: 1712 1443 1359 1045 274 156 138 115 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3294 3326 3368 3400 6366 6398 3294 Size: 32 42 32 2966 32 3302 Fragments arranged by size: 3302 2966 42 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6406 XbaI T'CTAG_A Cuts at: 3742 3742 Size: 6406 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6406 XmaI C'CCGG_G Cuts at: 1870 3731 1870 Size: 1861 4545 XmnI GAAnn'nnTTC Cuts at: 4535 4535 Size: 6406 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4214 276 Size: 53 83 186 936 2680 2468 Fragments arranged by size: 2680 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI DraIII EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SfiI SfoI SrfI SwaI XcmI