Restriction map for
pFN21AA1741
##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII AccIII AclI AflII AflIII
AgeI Alw44I ApaLI AscI AseI
AsnI Asp700I AspI AviII AvrII
BciVI BclI BfrI BlnI BsaBI
BsaWI BseAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrGI BssHII Bst1107I
BstZ17I ClaI CpoI DraI EagI
EclXI Eco52I EcoRV EcoT22I FbaI
FspI HpaI MamI MfeI MluI
MroI MunI NdeI NotI NsiI
PacI PaeR7I PflFI PinAI PmeI
PshBI Psp1406I PvuI RcaI RsrII
SalI SbfI ScaI SexAI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tth111I XbaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AccI 9 GTCTAC GT^[AC][GT]AC
AhdI 2125 GACACACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 2125 GACACACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 494 TGGCCA TGG^CCA
BamHI 3067 GGATCC G^GATCC
BbeI 4044 GGCGCC GGCGC^C
BbrPI 3100 CACGTG CAC^GTG
BglII 2178 AGATCT A^GATCT
BsaAI 3100 CACGTG [CT]AC^GT[GA]
BsiEI 537 CGACCG CG[GA][CT]^CG
BssSI 1443 CACGAG CACGAG(-5/-1)
BstAPI 4465 GCAGGTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3963 GGTGACC G^GT[ACGT]ACC
BstPI 3963 GGTGACC G^GT[ACGT]ACC
Eam1105I 2125 GACACACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 3963 GGTGACC G^GT[ACGT]ACC
EcoRI 2596 GAATTC G^AATTC
FseI 3049 GGCCGGCC GGCCGG^CC
KasI 4044 GGCGCC G^GCGCC
KspI 5045 CCGCGG CCGC^GG
MluNI 494 TGGCCA TGG^CCA
MscI 494 TGGCCA TGG^CCA
MslI 474 CACCACGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 3050 GCCGGC GCC^GGC
NarI 4044 GGCGCC GG^CGCC
NgoMIV 3050 GCCGGC G^CCGGC
NheI 3157 GCTAGC G^CTAGC
NruI 583 TCGCGA TCG^CGA
PmaCI 3100 CACGTG CAC^GTG
PmlI 3100 CACGTG CAC^GTG
SacII 5045 CCGCGG CCGC^GG
SfiI 4429 GGCCTTGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 4044 GGCGCC GGC^GCC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
Acc65I 2620 GGTACC G^GTACC
4774 GGTACC
AcyI 2911 GACGCC G[GA]^CG[CT]C
4044 GGCGCC
Aor51HI 461 AGCGCT AGC^GCT
2863 AGCGCT
Asp718I 2620 GGTACC G^GTACC
4774 GGTACC
BcgI 772 GCAGCCAGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4795 GCAGCAGACTCG
BsaHI 2911 GACGCC G[GA]^CG[CT]C
4044 GGCGCC
BsrFI 3050 GCCGGC [GA]^CCGG[CT]
3616 ACCGGC
BstBI 1426 TTCGAA TT^CGAA
5058 TTCGAA
Cfr10I 3050 GCCGGC [GA]^CCGG[CT]
3616 ACCGGC
DraIII 2078 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
3439 CACTTTGTG
DrdI 3771 GACTGACTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4899 GACACGGATGTC
Eco47III 461 AGCGCT AGC^GCT
2863 AGCGCT
HgaI 1243 GACGC GACGC(5/10)
2911 GACGC
Hin1I 2911 GACGCC G[GA]^CG[CT]C
4044 GGCGCC
HincII 1679 GTCAAC GT[CT]^[GA]AC
4933 GTCAAC
HindII 1679 GTCAAC GT[CT]^[GA]AC
4933 GTCAAC
HindIII 971 AAGCTT A^AGCTT
2925 AAGCTT
KpnI 2620 GGTACC GGTAC^C
4774 GGTACC
NcoI 32 CCATGG C^CATGG
2742 CCATGG
NspI 245 ACATGC [GA]CATG^[CT]
1646 GCATGT
NspV 1426 TTCGAA TT^CGAA
5058 TTCGAA
PshAI 1793 GACAGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2296 GACCCAGGTC
SacI 2946 GAGCTC GAGCT^C
4060 GAGCTC
SfuI 1426 TTCGAA TT^CGAA
5058 TTCGAA
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI AocI ApaI ApoI
AspHI AvaI AvaII BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PflMI PleI PpuMI PstI
PvuII RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI