(Circular) (Six-base) MAPSORT of: pFN21AA1741.seq Check: 4726 from: 1 to: 9892 pFN21AA1741 9892 bp With 182 enzymes: SgfI * October 20, 2009 10:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4371 6318 6461 4371 Size: 1947 143 7802 Fragments arranged by size: 7802 1947 143 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7702 278 Size: 53 83 186 936 6166 2468 Fragments arranged by size: 6166 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2015 7235 1937 Size: 78 5220 4594 Fragments arranged by size: 5220 4594 78 Acc65I G'GTAC_C Cuts at: 4625 6779 7213 4625 Size: 2154 434 7304 Fragments arranged by size: 7304 2154 434 AclI AA'CG_TT Cuts at: 1563 8019 8392 1563 Size: 6456 373 3063 Fragments arranged by size: 6456 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2638 2994 3312 3528 4145 5667 6570 6849 Size: 1006 356 318 216 617 1522 903 279 Cuts at: 6849 6992 7196 7955 9339 9607 1632 Size: 143 204 759 1384 268 1917 Fragments arranged by size: 1917 1522 1384 1006 903 759 617 356 318 279 268 216 204 143 AfeI AGC'GCT Cuts at: 2468 4870 2468 Size: 2402 7490 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 9695 AflIII A'CryG_T Cuts at: 1203 8791 9768 1203 Size: 7588 977 1327 Fragments arranged by size: 7588 1327 977 AgeI A'CCGG_T Cuts at: 8723 8723 Size: 9892 AhdI GACnn_n'nnGTC Cuts at: 4135 8621 4135 Size: 4486 5406 AleI CACnn'nnGTG Cuts at: 1166 2483 1166 Size: 1317 8575 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 9892 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4523 4555 4523 Size: 32 9860 AlwNI CAG_nnn'CTG Cuts at: 2900 2956 3884 3934 4037 5987 6281 6367 6476 Size: 56 928 50 103 1950 294 86 109 Cuts at: 6476 7635 9207 2900 Size: 1159 1572 3585 Fragments arranged by size: 3585 1950 1572 1159 928 294 109 103 86 56 50 ApaI G_GGCC'C Cuts at: 2143 5216 6760 2143 Size: 3073 1544 5275 Fragments arranged by size: 5275 3073 1544 ApaLI G'TGCA_C Cuts at: 7949 9105 7949 Size: 1156 8736 ApoI r'AATT_y Cuts at: 1493 4196 4601 4664 4994 7201 7427 7579 9688 Size: 2703 405 63 330 2207 226 152 2109 Cuts at: 9688 1493 Size: 1697 Fragments arranged by size: 2703 2207 2109 1697 405 330 226 152 63 AseI AT'TA_AT Cuts at: 160 1032 8446 160 Size: 872 7414 1606 Fragments arranged by size: 7414 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9892 AvaI C'yCGr_G Cuts at: 1870 1943 2055 2106 2136 2208 3064 3457 3966 Size: 73 112 51 30 72 856 393 509 Cuts at: 3966 4022 4967 5123 6430 6852 7217 1870 Size: 56 945 156 1307 422 365 4545 Fragments arranged by size: 4545 1307 945 856 509 422 393 365 156 112 73 72 56 51 30 BamHI G'GATC_C Cuts at: 5072 7222 7589 5072 Size: 2150 367 7375 Fragments arranged by size: 7375 2150 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2077 2185 4625 4817 6049 Size: 325 217 627 290 108 2440 192 1232 Cuts at: 6049 6330 6572 6750 6779 6856 6920 7213 8668 Size: 281 242 178 29 77 64 293 1455 Cuts at: 8668 618 Size: 1842 Fragments arranged by size: 2440 1842 1455 1232 627 325 293 290 281 242 217 192 178 108 77 64 29 BanII G_rGCy'C Cuts at: 1391 2143 3047 3072 3088 3135 3341 3717 3789 Size: 752 904 25 16 47 206 376 72 Cuts at: 3789 4955 5209 5216 5546 6069 6079 6132 6584 Size: 1166 254 7 330 523 10 53 452 Cuts at: 6584 6760 6962 7211 1391 Size: 176 202 249 4072 Fragments arranged by size: 4072 1166 904 752 523 452 376 330 254 249 206 202 176 72 53 47 25 16 10 7 BbeI G_GCGC'C Cuts at: 6053 6053 Size: 9892 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 5028 5468 6246 6567 928 Size: 971 3129 440 778 321 4253 Fragments arranged by size: 4253 3129 971 778 440 321 BbvCI CC'TCA_GC Cuts at: 3534 5721 6305 6964 7005 3534 Size: 2187 584 659 41 6421 Fragments arranged by size: 6421 2187 659 584 41 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2766 2800 6789 6823 8083 8117 1903 Size: 34 829 34 3989 34 1260 34 3678 Fragments arranged by size: 3989 3678 1260 829 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 7784 8994 7784 Size: 1210 8682 BclI T'GATC_A Cuts at: 1548 1548 Size: 9892 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2194 3083 3518 Size: 107 122 71 631 605 522 889 435 Cuts at: 3518 3527 5882 6441 6974 7156 8503 136 Size: 9 2355 559 533 182 1347 1525 Fragments arranged by size: 2355 1525 1347 889 631 605 559 533 522 435 182 122 107 71 9 BglII A'GATC_T Cuts at: 4183 9887 4183 Size: 5704 4188 BlpI GC'TnA_GC Cuts at: 2032 4119 7000 7309 2032 Size: 2087 2881 309 4615 Fragments arranged by size: 4615 2881 2087 309 Bme1580I G_kGCm'C Cuts at: 1790 2143 3737 4565 5122 5216 6125 6335 6753 Size: 353 1594 828 557 94 909 210 418 Cuts at: 6753 6760 6923 7953 9109 1790 Size: 7 163 1030 1156 2573 Fragments arranged by size: 2573 1594 1156 1030 909 828 557 418 353 210 163 94 7 BmgBI CAC'GTC Cuts at: 1318 6565 1318 Size: 5247 4645 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2364 3306 4080 Size: 416 53 83 399 24 947 942 774 Cuts at: 4080 4543 4631 4669 4795 5944 6657 7097 7623 Size: 463 88 38 126 1149 713 440 526 Cuts at: 7623 8581 442 Size: 958 1753 Fragments arranged by size: 1753 1149 958 947 942 774 713 526 463 440 416 399 126 88 83 53 38 24 BmtI G_CTAG'C Cuts at: 1056 5166 1056 Size: 4110 5782 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3705 4698 6320 7144 3705 Size: 993 1622 824 6453 Fragments arranged by size: 6453 1622 993 824 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2352 2673 3072 3287 3433 3869 4014 4134 Size: 1113 321 399 215 146 436 145 120 Cuts at: 4134 4555 4574 4888 5350 5919 6009 6188 6392 Size: 421 19 314 462 569 90 179 204 Cuts at: 6392 6515 6544 8552 1239 Size: 123 29 2008 2579 Fragments arranged by size: 2579 2008 1113 569 462 436 421 399 321 314 215 204 179 146 145 123 120 90 29 19 Bpu10I CC'TnA_GC Cuts at: 2772 2893 3534 3918 4949 5208 5597 5721 6305 Size: 121 641 384 1031 259 389 124 584 Cuts at: 6305 6956 6964 7005 2772 Size: 651 8 41 5659 Fragments arranged by size: 5659 1031 651 641 584 389 384 259 124 121 41 8 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2809 3731 3984 5623 8017 8882 9180 2809 Size: 922 253 1639 2394 865 298 3521 Fragments arranged by size: 3521 2394 1639 922 865 298 253 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3968 4206 4293 4313 4936 5127 5507 Size: 617 2469 238 87 20 623 191 380 Cuts at: 5507 6171 6221 8555 882 Size: 664 50 2334 2219 Fragments arranged by size: 2469 2334 2219 664 623 617 380 238 191 87 50 20 BsaAI yAC'GTr Cuts at: 493 1204 5107 7683 493 Size: 711 3903 2576 2702 Fragments arranged by size: 3903 2702 2576 711 BsaBI GATnn'nnATC Cuts at: 9886 9886 Size: 9892 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4917 6050 7699 8081 Size: 53 83 186 936 3384 1133 1649 382 Cuts at: 8081 8716 275 Size: 635 1451 Fragments arranged by size: 3384 1649 1451 1133 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 8325 8723 8997 9144 9555 8325 Size: 398 274 147 411 8662 Fragments arranged by size: 8662 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2608 2638 3029 3059 7093 Size: 30 165 30 1205 30 391 30 4034 Cuts at: 7093 7123 1178 Size: 30 3947 Fragments arranged by size: 4034 3947 1205 391 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2081 2084 2349 2378 2393 2741 2746 2830 Size: 895 3 265 29 15 348 5 84 Cuts at: 2830 3245 3248 3779 3959 3962 4311 4415 5377 Size: 415 3 531 180 3 349 104 962 Cuts at: 5377 5751 6238 6389 6467 6500 7022 1186 Size: 374 487 151 78 33 522 4056 Fragments arranged by size: 4056 962 895 531 522 487 415 374 349 348 265 180 151 104 84 78 33 29 15 5 3 3 3 BseYI C'CCAG_C Cuts at: 2174 2265 2369 2401 2455 3059 3104 3331 3596 Size: 91 104 32 54 604 45 227 265 Cuts at: 3596 3697 4223 4491 4977 5259 5292 5497 6199 Size: 101 526 268 486 282 33 205 702 Cuts at: 6199 9095 9591 2174 Size: 2896 496 2475 Fragments arranged by size: 2896 2475 702 604 526 496 486 282 268 265 227 205 104 101 91 54 45 33 32 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2433 3045 3653 1163 Size: 573 697 612 608 7402 Fragments arranged by size: 7402 697 612 608 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2545 7342 8103 9131 1540 Size: 275 186 544 4797 761 1028 2301 Fragments arranged by size: 4797 2301 1028 761 544 275 186 BsiHKAI G_wGCw'C Cuts at: 2712 3303 4364 4458 4955 6069 7953 8038 9109 Size: 591 1061 94 497 1114 1884 85 1071 Cuts at: 9109 2712 Size: 3495 Fragments arranged by size: 3495 1884 1114 1071 1061 591 497 94 85 BsmI GAATG_Cn' Cuts at: 7403 7496 7403 Size: 93 9799 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2143 2712 3047 3072 3088 3135 3303 Size: 399 353 569 335 25 16 47 168 Cuts at: 3303 3341 3717 3737 3789 4364 4458 4565 4955 Size: 38 376 20 52 575 94 107 390 Cuts at: 4955 5122 5209 5216 5546 6069 6079 6125 6132 Size: 167 87 7 330 523 10 46 7 Cuts at: 6132 6335 6584 6753 6760 6923 6962 7211 7953 Size: 203 249 169 7 163 39 249 742 Cuts at: 7953 8038 9109 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 575 569 523 399 390 376 353 335 330 249 249 203 169 168 167 163 107 94 87 85 52 47 46 39 38 25 20 16 10 7 7 7 BspEI T'CCGG_A Cuts at: 9555 9555 Size: 9892 BspHI T'CATG_A Cuts at: 7781 9511 7781 Size: 1730 8162 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2803 4157 4371 6318 6461 7247 844 Size: 1959 1354 214 1947 143 786 3489 Fragments arranged by size: 3489 1959 1947 1354 786 214 143 BsrBI CCG'CTC Cuts at: 1394 1823 7779 1394 Size: 429 5956 3507 Fragments arranged by size: 5956 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 8387 8561 65 Size: 1183 7139 174 1396 Fragments arranged by size: 7139 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5055 5621 8536 8723 1700 Size: 110 3245 566 2915 187 2869 Fragments arranged by size: 3245 2915 2869 566 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 9892 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9892 BssSI C'ACGA_G Cuts at: 3448 7952 8964 3448 Size: 4504 1012 4376 Fragments arranged by size: 4504 4376 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 6476 794 Size: 5682 4210 BstBI TT'CG_AA Cuts at: 3432 7064 8696 3432 Size: 3632 1632 4628 Fragments arranged by size: 4628 3632 1632 BstEII G'GTnAC_C Cuts at: 1184 5968 1184 Size: 4784 5108 BstXI CCAn_nnnn'nTGG Cuts at: 2182 2273 2354 2485 3271 5530 6199 6406 6522 Size: 91 81 131 786 2259 669 207 116 Cuts at: 6522 2182 Size: 5552 Fragments arranged by size: 5552 2259 786 669 207 131 116 91 81 BstYI r'GATC_y Cuts at: 1972 2493 3199 4183 5072 6656 6696 7222 7589 Size: 521 706 984 889 1584 40 526 367 Cuts at: 7589 7974 7991 9443 9529 9541 9887 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1584 1452 984 889 706 526 521 385 367 346 86 40 17 12 Bsu36I CC'TnA_GG Cuts at: 2724 2887 3120 5954 6137 7129 2724 Size: 163 233 2834 183 992 5487 Fragments arranged by size: 5487 2834 992 233 183 163 BtgI C'CryG_G Cuts at: 513 1065 2037 2481 4747 5568 6842 7050 513 Size: 552 972 444 2266 821 1274 208 3355 Fragments arranged by size: 3355 2266 1274 972 821 552 444 208 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3687 487 Size: 683 56 2461 6692 Fragments arranged by size: 6692 2461 683 56 BtsI GCAGTG_nn' Cuts at: 2048 2619 3932 4897 5097 5227 7408 8202 8222 Size: 571 1313 965 200 130 2181 794 20 Cuts at: 8222 2048 Size: 3718 Fragments arranged by size: 3718 2181 1313 965 794 571 200 130 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9892 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2373 2408 529 Size: 35 1809 35 8013 Fragments arranged by size: 8013 1809 35 35 DraI TTT'AAA Cuts at: 7196 7196 Size: 9892 DraIII CAC_nnn'GTG Cuts at: 4088 5449 4088 Size: 1361 8531 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5782 6910 8899 809 Size: 507 4466 1128 1989 1802 Fragments arranged by size: 4466 1989 1802 1128 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2475 2499 3549 Size: 54 1425 50 275 18 645 24 1050 Cuts at: 3549 4393 5053 5057 7339 8228 9857 8 Size: 844 660 4 2282 889 1629 43 Fragments arranged by size: 2282 1629 1425 1050 889 844 660 645 275 54 50 43 24 18 4 EagI C'GGCC_G Cuts at: 1537 1812 7339 1537 Size: 275 5527 4090 Fragments arranged by size: 5527 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2484 2657 2700 4667 4730 4793 4859 5643 Size: 1131 173 43 1967 63 63 66 784 Cuts at: 5643 5699 6589 6867 7822 1353 Size: 56 890 278 955 3423 Fragments arranged by size: 3423 1967 1131 955 890 784 278 173 66 63 63 56 43 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2441 3248 8479 8853 8999 387 Size: 1064 138 852 807 5231 374 146 1280 Fragments arranged by size: 5231 1280 1064 852 807 374 146 138 EcoICRI GAG'CTC Cuts at: 4953 6067 4953 Size: 1114 8778 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2352 2638 2673 2994 3072 3287 3312 Size: 393 720 286 35 321 78 215 25 Cuts at: 3312 3433 3528 3869 4014 4134 4145 4555 4574 Size: 121 95 341 145 120 11 410 19 Cuts at: 4574 4888 5350 5667 5919 6009 6188 6392 6515 Size: 314 462 317 252 90 179 204 123 Cuts at: 6515 6544 6570 6849 6992 7196 7955 8552 9339 Size: 29 26 279 143 204 759 597 787 Cuts at: 9339 9607 1239 Size: 268 1524 Fragments arranged by size: 1524 787 759 720 597 462 410 393 341 321 317 314 286 279 268 252 215 204 204 179 145 143 123 121 120 95 90 78 35 29 26 25 19 11 EcoNI CCTnn'n_nnAGG Cuts at: 2771 3179 3863 5033 6371 6449 6523 6794 6824 Size: 408 684 1170 1338 78 74 271 30 Cuts at: 6824 2771 Size: 5839 Fragments arranged by size: 5839 1338 1170 684 408 271 78 74 30 EcoO109I rG'GnC_Cy Cuts at: 2140 2213 2261 2437 2527 2604 2764 2855 2897 Size: 73 48 176 90 77 160 91 42 Cuts at: 2897 3094 3453 4034 4908 5212 5213 6364 6442 Size: 197 359 581 874 304 1 1151 78 Cuts at: 6442 6644 6757 7078 2140 Size: 202 113 321 4954 Fragments arranged by size: 4954 1151 874 581 359 321 304 202 197 176 160 113 91 90 78 77 73 48 42 1 EcoRI G'AATT_C Cuts at: 4601 7201 7579 4601 Size: 2600 378 6914 Fragments arranged by size: 6914 2600 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 5045 5045 Size: 9892 FseI GG_CCGG'CC Cuts at: 5059 5059 Size: 9892 FspI TGC'GCA Cuts at: 8398 8398 Size: 9892 HaeII r_GCGC'y Cuts at: 1678 2290 2470 3283 4872 6053 9039 1678 Size: 612 180 813 1589 1181 2986 2531 Fragments arranged by size: 2986 2531 1589 1181 813 612 180 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2570 2602 2608 2640 3029 Size: 32 135 32 998 32 6 32 389 Cuts at: 3029 3061 3982 4014 4121 4153 5057 5089 5247 Size: 32 921 32 107 32 904 32 158 Cuts at: 5247 5279 5353 5385 5969 6001 6233 6265 7016 Size: 32 74 32 584 32 232 32 751 Cuts at: 7016 7048 8533 8565 8607 8639 1373 Size: 32 1485 32 42 32 2626 Fragments arranged by size: 2626 1485 998 921 904 751 584 389 232 158 135 107 74 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 6 HincII GTy'rAC Cuts at: 669 1657 1938 3686 6940 7236 7482 669 Size: 988 281 1748 3254 296 246 3079 Fragments arranged by size: 3254 3079 1748 988 296 281 246 HindIII A'AGCT_T Cuts at: 748 2976 4930 748 Size: 2228 1954 5710 Fragments arranged by size: 5710 2228 1954 HpaI GTT'AAC Cuts at: 7482 7482 Size: 9892 KasI G'GCGC_C Cuts at: 6049 6049 Size: 9892 KpnI G_GTAC'C Cuts at: 4629 6783 7217 4629 Size: 2154 434 7304 Fragments arranged by size: 7304 2154 434 MfeI C'AATT_G Cuts at: 7491 7491 Size: 9892 MluI A'CGCG_T Cuts at: 9768 9768 Size: 9892 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2769 3432 4459 5812 6429 6774 9006 9190 Size: 1464 663 1027 1353 617 345 2232 184 Cuts at: 9190 1305 Size: 2007 Fragments arranged by size: 2232 2007 1464 1353 1027 663 617 345 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2501 10 Size: 54 1425 343 669 7401 Fragments arranged by size: 7401 1425 669 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2483 7850 8209 8368 518 Size: 648 843 474 5367 359 159 2042 Fragments arranged by size: 5367 2042 843 648 474 359 159 MspA1I CmG'CkG Cuts at: 1606 3002 3701 4053 4245 4507 5145 5471 5497 Size: 1396 699 352 192 262 638 326 26 Cuts at: 5497 5526 6425 7004 7052 7308 7985 9133 9378 Size: 29 899 579 48 256 677 1148 245 Cuts at: 9378 1606 Size: 2120 Fragments arranged by size: 2120 1396 1148 899 699 677 638 579 352 326 262 256 245 192 48 29 26 NaeI GCC'GGC Cuts at: 5057 5057 Size: 9892 NarI GG'CG_CC Cuts at: 6050 6050 Size: 9892 NcoI C'CATG_G Cuts at: 513 1065 2037 4747 513 Size: 552 972 2710 5658 Fragments arranged by size: 5658 2710 972 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 9892 NgoMIV G'CCGG_C Cuts at: 5055 5055 Size: 9892 NheI G'CTAG_C Cuts at: 1052 5162 1052 Size: 4110 5782 NotI GC'GGCC_GC Cuts at: 7339 7339 Size: 9892 NruI TCG'CGA Cuts at: 2590 2590 Size: 9892 NspI r_CATG'y Cuts at: 1132 1229 2254 3655 7250 8795 1132 Size: 97 1025 1401 3595 1545 2229 Fragments arranged by size: 3595 2229 1545 1401 1025 97 PasI CC'CwG_GG Cuts at: 1348 2420 2628 4448 4558 5315 5879 6232 6361 Size: 1072 208 1820 110 757 564 353 129 Cuts at: 6361 1348 Size: 4879 Fragments arranged by size: 4879 1820 1072 757 564 353 208 129 110 PciI A'CATG_T Cuts at: 8791 8791 Size: 9892 PflMI CCAn_nnn'nTGG Cuts at: 1108 2814 3142 4088 4371 4729 5173 5987 6046 Size: 1706 328 946 283 358 444 814 59 Cuts at: 6046 6061 1108 Size: 15 4939 Fragments arranged by size: 4939 1706 946 814 444 358 328 283 59 15 PfoI T'CCnGG_A Cuts at: 3846 4590 4904 5896 6521 6825 3846 Size: 744 314 992 625 304 6913 Fragments arranged by size: 6913 992 744 625 314 304 PmeI GTTT'AAAC Cuts at: 7196 7196 Size: 9892 PmlI CAC'GTG Cuts at: 5107 5107 Size: 9892 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7961 7993 9474 9506 7961 Size: 32 1481 32 8347 Fragments arranged by size: 8347 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2213 2261 2527 2604 2764 4908 7078 2213 Size: 48 266 77 160 2144 2170 5027 Fragments arranged by size: 5027 2170 2144 266 160 77 48 PshAI GACnn'nnGTC Cuts at: 1613 3802 4305 1613 Size: 2189 503 7200 Fragments arranged by size: 7200 2189 503 PsiI TTA'TAA Cuts at: 7462 7462 Size: 9892 PspOMI G'GGCC_C Cuts at: 2139 5212 6756 2139 Size: 3073 1544 5275 Fragments arranged by size: 5275 3073 1544 PstI C_TGCA'G Cuts at: 830 4191 5894 6277 7244 830 Size: 3361 1703 383 967 3478 Fragments arranged by size: 3478 3361 1703 967 383 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9892 PvuII CAG'CTG Cuts at: 3701 4245 5145 5471 5497 5526 7004 3701 Size: 544 900 326 26 29 1478 6589 Fragments arranged by size: 6589 1478 900 544 326 29 26 SacI G_AGCT'C Cuts at: 4955 6069 4955 Size: 1114 8778 SacII CC_GC'GG Cuts at: 7053 7053 Size: 9892 SalI G'TCGA_C Cuts at: 1936 7234 1936 Size: 5298 4594 SanDI GG'GwC_CC Cuts at: 2213 2213 Size: 9892 SapI GCTCTTCn'nnn_ Cuts at: 2700 4859 5643 5699 6589 2700 Size: 2159 784 56 890 6003 Fragments arranged by size: 6003 2159 890 784 56 SbfI CC_TGCA'GG Cuts at: 7244 7244 Size: 9892 ScaI AGT'ACT Cuts at: 8140 9852 8140 Size: 1712 8180 SfcI C'TryA_G Cuts at: 826 1045 4187 4630 4885 5890 6273 6834 7240 Size: 219 3142 443 255 1005 383 561 406 Cuts at: 7240 8375 9056 9247 826 Size: 1135 681 191 1471 Fragments arranged by size: 3142 1471 1135 1005 681 561 443 406 383 255 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 6441 6441 Size: 9892 SfoI GGC'GCC Cuts at: 6051 6051 Size: 9892 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9892 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9892 SmaI CCC'GGG Cuts at: 1872 2057 2138 2210 3066 3968 7219 1872 Size: 185 81 72 856 902 3251 4545 Fragments arranged by size: 4545 3251 902 856 185 81 72 SmlI C'TyrA_G Cuts at: 820 1017 1943 2824 3710 3999 5602 7996 8897 Size: 197 926 881 886 289 1603 2394 901 Cuts at: 8897 9159 820 Size: 262 1553 Fragments arranged by size: 2394 1603 1553 926 901 886 881 289 262 197 SnaBI TAC'GTA Cuts at: 493 7683 493 Size: 7190 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 9892 SphI G_CATG'C Cuts at: 1132 7250 1132 Size: 6118 3774 SrfI GCCC'GGGC Cuts at: 3968 3968 Size: 9892 SspI AAT'ATT Cuts at: 5 52 7816 5 Size: 47 7764 2081 Fragments arranged by size: 7764 2081 47 StuI AGG'CCT Cuts at: 1517 2311 2324 2871 1517 Size: 794 13 547 8538 Fragments arranged by size: 8538 794 547 13 StyI C'CwwG_G Cuts at: 513 1065 2037 2306 3240 3358 3789 4165 4276 Size: 552 972 269 934 118 431 376 111 Cuts at: 4276 4651 4747 5029 5857 6183 6258 6399 6436 Size: 375 96 282 828 326 75 141 37 Cuts at: 6436 6640 6783 6924 7331 513 Size: 204 143 141 407 3074 Fragments arranged by size: 3074 972 934 828 552 431 407 376 375 326 282 269 204 143 141 141 118 111 96 75 37 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2360 3176 3325 3619 3677 3995 4099 Size: 1255 780 816 149 294 58 318 104 Cuts at: 4099 4676 7919 7936 8089 8274 8719 325 Size: 577 3243 17 153 185 445 1498 Fragments arranged by size: 3243 1498 1255 816 780 577 445 318 294 185 153 149 104 58 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 8138 9850 96 Size: 274 80 33 51 1443 6161 1712 138 Fragments arranged by size: 6161 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6003 6035 6807 6839 7093 7125 9852 9884 6003 Size: 32 772 32 254 32 2727 32 6011 Fragments arranged by size: 6011 2727 772 254 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 9892 XbaI T'CTAG_A Cuts at: 7228 7228 Size: 9892 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2273 3671 5111 6181 2273 Size: 1398 1440 1070 5984 Fragments arranged by size: 5984 1440 1398 1070 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 9892 XmaI C'CCGG_G Cuts at: 1870 2055 2136 2208 3064 3966 7217 1870 Size: 185 81 72 856 902 3251 4545 Fragments arranged by size: 4545 3251 902 856 185 81 72 XmnI GAAnn'nnTTC Cuts at: 8021 8021 Size: 9892 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7700 276 Size: 53 83 186 936 6166 2468 Fragments arranged by size: 6166 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I EcoRV FspAI NsiI PacI PspXI PsrI RsrII SexAI SwaI