Restriction map for
pFN21AA1741
(Linear) (Six-base) MAPSORT of: KIAA1741.seq Check: 3050 from: 1 to: 5187
KIAA1741.orf
With 182 enzymes: SgfI *
October 24, 2006 15:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2366 4313 4456 5187
Size: 2366 1947 143 731
Fragments arranged by size:
2366 1947 731 143
AccI GT'mk_AC
Cuts at: 0 10 5187
Size: 10 5177
Acc65I G'GTAC_C
Cuts at: 0 2620 4774 5187
Size: 2620 2154 413
Fragments arranged by size:
2620 2154 413
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 633 989 1307 1523 2140 3662 4565 4844
Size: 633 356 318 216 617 1522 903 279
Cuts at: 4844 4987 5187
Size: 143 200
Fragments arranged by size:
1522 903 633 617 356 318 279 216
200 143
AfeI AGC'GCT
Cuts at: 0 463 2865 5187
Size: 463 2402 2322
Fragments arranged by size:
2402 2322 463
AhdI GACnn_n'nnGTC
Cuts at: 0 2130 5187
Size: 2130 3057
AleI CACnn'nnGTG
Cuts at: 0 478 5187
Size: 478 4709
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2518 2550 5187
Size: 2518 32 2637
Fragments arranged by size:
2637 2518 32
AlwNI CAG_nnn'CTG
Cuts at: 0 895 951 1879 1929 2032 3982 4276 4362
Size: 895 56 928 50 103 1950 294 86
Cuts at: 4362 4471 5187
Size: 109 716
Fragments arranged by size:
1950 928 895 716 294 109 103 86
56 50
ApaI G_GGCC'C
Cuts at: 0 138 3211 4755 5187
Size: 138 3073 1544 432
Fragments arranged by size:
3073 1544 432 138
ApoI r'AATT_y
Cuts at: 0 2191 2596 2659 2989 5187
Size: 2191 405 63 330 2198
Fragments arranged by size:
2198 2191 405 330 63
AvaI C'yCGr_G
Cuts at: 0 50 101 131 203 1059 1452 1961 2017
Size: 50 51 30 72 856 393 509 56
Cuts at: 2017 2962 3118 4425 4847 5187
Size: 945 156 1307 422 340
Fragments arranged by size:
1307 945 856 509 422 393 340 156
72 56 51 50 30
BamHI G'GATC_C
Cuts at: 0 3067 5187
Size: 3067 2120
BanI G'GyrC_C
Cuts at: 0 72 180 2620 2812 4044 4325 4567 4745
Size: 72 108 2440 192 1232 281 242 178
Cuts at: 4745 4774 4851 4915 5187
Size: 29 77 64 272
Fragments arranged by size:
2440 1232 281 272 242 192 178 108
77 72 64 29
BanII G_rGCy'C
Cuts at: 0 138 1042 1067 1083 1130 1336 1712 1784
Size: 138 904 25 16 47 206 376 72
Cuts at: 1784 2950 3204 3211 3541 4064 4074 4127 4579
Size: 1166 254 7 330 523 10 53 452
Cuts at: 4579 4755 4957 5187
Size: 176 202 230
Fragments arranged by size:
1166 904 523 452 376 330 254 230
206 202 176 138 72 53 47 25
16 10 7
BbeI G_GCGC'C
Cuts at: 0 4048 5187
Size: 4048 1139
BbsI GAAGACnn'nnnn_
Cuts at: 0 3023 3463 4241 4562 5187
Size: 3023 440 778 321 625
Fragments arranged by size:
3023 778 625 440 321
BbvCI CC'TCA_GC
Cuts at: 0 1529 3716 4300 4959 5000 5187
Size: 1529 2187 584 659 41 187
Fragments arranged by size:
2187 1529 659 584 187 41
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 761 795 4784 4818 5187
Size: 761 34 3989 34 369
Fragments arranged by size:
3989 761 369 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 189 1078 1513 1522 3877 4436 4969 5151
Size: 189 889 435 9 2355 559 533 182
Cuts at: 5151 5187
Size: 36
Fragments arranged by size:
2355 889 559 533 435 189 182 36
9
BglII A'GATC_T
Cuts at: 0 2178 5187
Size: 2178 3009
BlpI GC'TnA_GC
Cuts at: 0 27 2114 4995 5187
Size: 27 2087 2881 192
Fragments arranged by size:
2881 2087 192 27
Bme1580I G_kGCm'C
Cuts at: 0 138 1732 2560 3117 3211 4120 4330 4748
Size: 138 1594 828 557 94 909 210 418
Cuts at: 4748 4755 4918 5187
Size: 7 163 269
Fragments arranged by size:
1594 909 828 557 418 269 210 163
138 94 7
BmgBI CAC'GTC
Cuts at: 0 4560 5187
Size: 4560 627
BmrI ACTGGGnnnn_n'
Cuts at: 0 359 1301 2075 2538 2626 2664 2790 3939
Size: 359 942 774 463 88 38 126 1149
Cuts at: 3939 4652 5092 5187
Size: 713 440 95
Fragments arranged by size:
1149 942 774 713 463 440 359 126
95 88 38
BmtI G_CTAG'C
Cuts at: 0 3161 5187
Size: 3161 2026
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1700 2693 4315 5139 5187
Size: 1700 993 1622 824 48
Fragments arranged by size:
1700 1622 993 824 48
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 347 668 1067 1282 1428 1864 2009 2129
Size: 347 321 399 215 146 436 145 120
Cuts at: 2129 2550 2569 2883 3345 3914 4004 4183 4387
Size: 421 19 314 462 569 90 179 204
Cuts at: 4387 4510 4539 5187
Size: 123 29 648
Fragments arranged by size:
648 569 462 436 421 399 347 321
314 215 204 179 146 145 123 120
90 29 19
Bpu10I CC'TnA_GC
Cuts at: 0 767 888 1529 1913 2944 3203 3592 3716
Size: 767 121 641 384 1031 259 389 124
Cuts at: 3716 4300 4951 4959 5000 5187
Size: 584 651 8 41 187
Fragments arranged by size:
1031 767 651 641 584 389 384 259
187 124 121 41 8
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 804 1726 1979 3618 5187
Size: 804 922 253 1639 1569
Fragments arranged by size:
1639 1569 922 804 253
BsaI GGTCTCn'nnnn_
Cuts at: 0 1963 2201 2288 2308 2931 3122 3502 4166
Size: 1963 238 87 20 623 191 380 664
Cuts at: 4166 4216 5187
Size: 50 971
Fragments arranged by size:
1963 971 664 623 380 238 191 87
50 20
BsaAI yAC'GTr
Cuts at: 0 3102 5187
Size: 3102 2085
BsaHI Gr'CG_yC
Cuts at: 0 2912 4045 5187
Size: 2912 1133 1142
Fragments arranged by size:
2912 1142 1133
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 603 633 1024 1054 5088 5118 5187
Size: 603 30 391 30 4034 30 69
Fragments arranged by size:
4034 603 391 69 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 76 79 344 373 388 736 741 825
Size: 76 3 265 29 15 348 5 84
Cuts at: 825 1240 1243 1774 1954 1957 2306 2410 3372
Size: 415 3 531 180 3 349 104 962
Cuts at: 3372 3746 4233 4384 4462 4495 5017 5187
Size: 374 487 151 78 33 522 170
Fragments arranged by size:
962 531 522 487 415 374 349 348
265 180 170 151 104 84 78 76
33 29 15 5 3 3 3
BseYI C'CCAG_C
Cuts at: 0 169 260 364 396 450 1054 1099 1326
Size: 169 91 104 32 54 604 45 227
Cuts at: 1326 1591 1692 2218 2486 2972 3254 3287 3492
Size: 265 101 526 268 486 282 33 205
Cuts at: 3492 4194 5187
Size: 702 993
Fragments arranged by size:
993 702 604 526 486 282 268 265
227 205 169 104 101 91 54 45
33 32
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 428 1040 1648 5187
Size: 428 612 608 3539
Fragments arranged by size:
3539 612 608 428
BsiEI CG_ry'CG
Cuts at: 0 540 5187
Size: 540 4647
BsiHKAI G_wGCw'C
Cuts at: 0 707 1298 2359 2453 2950 4064 5187
Size: 707 591 1061 94 497 1114 1123
Fragments arranged by size:
1123 1114 1061 707 591 497 94
Bsp1286I G_dGCh'C
Cuts at: 0 138 707 1042 1067 1083 1130 1298 1336
Size: 138 569 335 25 16 47 168 38
Cuts at: 1336 1712 1732 1784 2359 2453 2560 2950 3117
Size: 376 20 52 575 94 107 390 167
Cuts at: 3117 3204 3211 3541 4064 4074 4120 4127 4330
Size: 87 7 330 523 10 46 7 203
Cuts at: 4330 4579 4748 4755 4918 4957 5187
Size: 249 169 7 163 39 230
Fragments arranged by size:
575 569 523 390 376 335 330 249
230 203 169 168 167 163 138 107
94 87 52 47 46 39 38 25
20 16 10 7 7 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 798 2152 2366 4313 4456 5187
Size: 798 1354 214 1947 143 731
Fragments arranged by size:
1947 1354 798 731 214 143
BsrFI r'CCGG_y
Cuts at: 0 3050 3616 5187
Size: 3050 566 1571
Fragments arranged by size:
3050 1571 566
BssSI C'ACGA_G
Cuts at: 0 1443 5187
Size: 1443 3744
BstAPI GCAn_nnn'nTGC
Cuts at: 0 4471 5187
Size: 4471 716
BstBI TT'CG_AA
Cuts at: 0 1427 5059 5187
Size: 1427 3632 128
Fragments arranged by size:
3632 1427 128
BstEII G'GTnAC_C
Cuts at: 0 3963 5187
Size: 3963 1224
BstXI CCAn_nnnn'nTGG
Cuts at: 0 177 268 349 480 1266 3525 4194 4401
Size: 177 91 81 131 786 2259 669 207
Cuts at: 4401 4517 5187
Size: 116 670
Fragments arranged by size:
2259 786 670 669 207 177 131 116
91 81
BstYI r'GATC_y
Cuts at: 0 488 1194 2178 3067 4651 4691 5187
Size: 488 706 984 889 1584 40 496
Fragments arranged by size:
1584 984 889 706 496 488 40
Bsu36I CC'TnA_GG
Cuts at: 0 719 882 1115 3949 4132 5124 5187
Size: 719 163 233 2834 183 992 63
Fragments arranged by size:
2834 992 719 233 183 163 63
BtgI C'CryG_G
Cuts at: 0 32 476 2742 3563 4837 5045 5187
Size: 32 444 2266 821 1274 208 142
Fragments arranged by size:
2266 1274 821 444 208 142 32
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1682 5187
Size: 1682 3505
BtsI GCAGTG_nn'
Cuts at: 0 43 614 1927 2892 3092 3222 5187
Size: 43 571 1313 965 200 130 1965
Fragments arranged by size:
1965 1313 965 571 200 130 43
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 368 403 5187
Size: 368 35 4784
Fragments arranged by size:
4784 368 35
DraIII CAC_nnn'GTG
Cuts at: 0 2083 3444 5187
Size: 2083 1361 1743
Fragments arranged by size:
2083 1743 1361
DrdI GACnn_nn'nnGTC
Cuts at: 0 3777 4905 5187
Size: 3777 1128 282
Fragments arranged by size:
3777 1128 282
EaeI y'GGCC_r
Cuts at: 0 470 494 1544 2388 3048 3052 5187
Size: 470 24 1050 844 660 4 2135
Fragments arranged by size:
2135 1050 844 660 470 24 4
EarI CTCTTCn'nnn_
Cuts at: 0 479 652 695 2662 2725 2788 2854 3638
Size: 479 173 43 1967 63 63 66 784
Cuts at: 3638 3694 4584 4862 5187
Size: 56 890 278 325
Fragments arranged by size:
1967 890 784 479 325 278 173 66
63 63 56 43
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 436 1243 5187
Size: 436 807 3944
Fragments arranged by size:
3944 807 436
EcoICRI GAG'CTC
Cuts at: 0 2948 4062 5187
Size: 2948 1114 1125
Fragments arranged by size:
2948 1125 1114
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 347 633 668 989 1067 1282 1307 1428
Size: 347 286 35 321 78 215 25 121
Cuts at: 1428 1523 1864 2009 2129 2140 2550 2569 2883
Size: 95 341 145 120 11 410 19 314
Cuts at: 2883 3345 3662 3914 4004 4183 4387 4510 4539
Size: 462 317 252 90 179 204 123 29
Cuts at: 4539 4565 4844 4987 5187
Size: 26 279 143 200
Fragments arranged by size:
462 410 347 341 321 317 314 286
279 252 215 204 200 179 145 143
123 121 120 95 90 78 35 29
26 25 19 11
EcoNI CCTnn'n_nnAGG
Cuts at: 0 766 1174 1858 3028 4366 4444 4518 4789
Size: 766 408 684 1170 1338 78 74 271
Cuts at: 4789 4819 5187
Size: 30 368
Fragments arranged by size:
1338 1170 766 684 408 368 271 78
74 30
EcoO109I rG'GnC_Cy
Cuts at: 0 135 208 256 432 522 599 759 850
Size: 135 73 48 176 90 77 160 91
Cuts at: 850 892 1089 1448 2029 2903 3207 3208 4359
Size: 42 197 359 581 874 304 1 1151
Cuts at: 4359 4437 4639 4752 5073 5187
Size: 78 202 113 321 114
Fragments arranged by size:
1151 874 581 359 321 304 202 197
176 160 135 114 113 91 90 78
77 73 48 42 1
EcoRI G'AATT_C
Cuts at: 0 2596 5187
Size: 2596 2591
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3040 5187
Size: 3040 2147
FseI GG_CCGG'CC
Cuts at: 0 3054 5187
Size: 3054 2133
HaeII r_GCGC'y
Cuts at: 0 285 465 1278 2867 4048 5187
Size: 285 180 813 1589 1181 1139
Fragments arranged by size:
1589 1181 1139 813 285 180
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 565 597 603 635 1024 1056 1977 2009
Size: 565 32 6 32 389 32 921 32
Cuts at: 2009 2116 2148 3052 3084 3242 3274 3348 3380
Size: 107 32 904 32 158 32 74 32
Cuts at: 3380 3964 3996 4228 4260 5011 5043 5187
Size: 584 32 232 32 751 32 144
Fragments arranged by size:
921 904 751 584 565 389 232 158
144 107 74 32 32 32 32 32
32 32 32 32 32 32 6
HincII GTy'rAC
Cuts at: 0 1681 4935 5187
Size: 1681 3254 252
Fragments arranged by size:
3254 1681 252
HindIII A'AGCT_T
Cuts at: 0 971 2925 5187
Size: 971 1954 2262
Fragments arranged by size:
2262 1954 971
KasI G'GCGC_C
Cuts at: 0 4044 5187
Size: 4044 1143
KpnI G_GTAC'C
Cuts at: 0 2624 4778 5187
Size: 2624 2154 409
Fragments arranged by size:
2624 2154 409
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 764 1427 2454 3807 4424 4769 5187
Size: 764 663 1027 1353 617 345 418
Fragments arranged by size:
1353 1027 764 663 617 418 345
MscI TGG'CCA
Cuts at: 0 496 5187
Size: 496 4691
MslI CAynn'nnrTG
Cuts at: 0 478 5187
Size: 478 4709
MspA1I CmG'CkG
Cuts at: 0 997 1696 2048 2240 2502 3140 3466 3492
Size: 997 699 352 192 262 638 326 26
Cuts at: 3492 3521 4420 4999 5047 5187
Size: 29 899 579 48 140
Fragments arranged by size:
997 899 699 638 579 352 326 262
192 140 48 29 26
NaeI GCC'GGC
Cuts at: 0 3052 5187
Size: 3052 2135
NarI GG'CG_CC
Cuts at: 0 4045 5187
Size: 4045 1142
NcoI C'CATG_G
Cuts at: 0 32 2742 5187
Size: 32 2710 2445
Fragments arranged by size:
2710 2445 32
NgoMIV G'CCGG_C
Cuts at: 0 3050 5187
Size: 3050 2137
NheI G'CTAG_C
Cuts at: 0 3157 5187
Size: 3157 2030
NruI TCG'CGA
Cuts at: 0 585 5187
Size: 585 4602
NspI r_CATG'y
Cuts at: 0 249 1650 5187
Size: 249 1401 3537
Fragments arranged by size:
3537 1401 249
PasI CC'CwG_GG
Cuts at: 0 415 623 2443 2553 3310 3874 4227 4356
Size: 415 208 1820 110 757 564 353 129
Cuts at: 4356 5187
Size: 831
Fragments arranged by size:
1820 831 757 564 415 353 208 129
110
PflMI CCAn_nnn'nTGG
Cuts at: 0 809 1137 2083 2366 2724 3168 3982 4041
Size: 809 328 946 283 358 444 814 59
Cuts at: 4041 4056 5187
Size: 15 1131
Fragments arranged by size:
1131 946 814 809 444 358 328 283
59 15
PfoI T'CCnGG_A
Cuts at: 0 1841 2585 2899 3891 4516 4820 5187
Size: 1841 744 314 992 625 304 367
Fragments arranged by size:
1841 992 744 625 367 314 304
PmlI CAC'GTG
Cuts at: 0 3102 5187
Size: 3102 2085
PpuMI rG'GwC_Cy
Cuts at: 0 208 256 522 599 759 2903 5073 5187
Size: 208 48 266 77 160 2144 2170 114
Fragments arranged by size:
2170 2144 266 208 160 114 77 48
PshAI GACnn'nnGTC
Cuts at: 0 1797 2300 5187
Size: 1797 503 2887
Fragments arranged by size:
2887 1797 503
PspOMI G'GGCC_C
Cuts at: 0 134 3207 4751 5187
Size: 134 3073 1544 436
Fragments arranged by size:
3073 1544 436 134
PstI C_TGCA'G
Cuts at: 0 2186 3889 4272 5187
Size: 2186 1703 383 915
Fragments arranged by size:
2186 1703 915 383
PvuII CAG'CTG
Cuts at: 0 1696 2240 3140 3466 3492 3521 4999 5187
Size: 1696 544 900 326 26 29 1478 188
Fragments arranged by size:
1696 1478 900 544 326 188 29 26
SacI G_AGCT'C
Cuts at: 0 2950 4064 5187
Size: 2950 1114 1123
Fragments arranged by size:
2950 1123 1114
SacII CC_GC'GG
Cuts at: 0 5048 5187
Size: 5048 139
SanDI GG'GwC_CC
Cuts at: 0 208 5187
Size: 208 4979
SapI GCTCTTCn'nnn_
Cuts at: 0 695 2854 3638 3694 4584 5187
Size: 695 2159 784 56 890 603
Fragments arranged by size:
2159 890 784 695 603 56
SfcI C'TryA_G
Cuts at: 0 2182 2625 2880 3885 4268 4829 5187
Size: 2182 443 255 1005 383 561 358
Fragments arranged by size:
2182 1005 561 443 383 358 255
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 4436 5187
Size: 4436 751
SfoI GGC'GCC
Cuts at: 0 4046 5187
Size: 4046 1141
SmaI CCC'GGG
Cuts at: 0 52 133 205 1061 1963 5187
Size: 52 81 72 856 902 3224
Fragments arranged by size:
3224 902 856 81 72 52
SmlI C'TyrA_G
Cuts at: 0 819 1705 1994 3597 5187
Size: 819 886 289 1603 1590
Fragments arranged by size:
1603 1590 886 819 289
SrfI GCCC'GGGC
Cuts at: 0 1963 5187
Size: 1963 3224
StuI AGG'CCT
Cuts at: 0 306 319 866 5187
Size: 306 13 547 4321
Fragments arranged by size:
4321 547 306 13
StyI C'CwwG_G
Cuts at: 0 32 301 1235 1353 1784 2160 2271 2646
Size: 32 269 934 118 431 376 111 375
Cuts at: 2646 2742 3024 3852 4178 4253 4394 4431 4635
Size: 96 282 828 326 75 141 37 204
Cuts at: 4635 4778 4919 5187
Size: 143 141 268
Fragments arranged by size:
934 828 431 376 375 326 282 269
268 204 143 141 141 118 111 96
75 37 32
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 355 1171 1320 1614 1672 1990 2094 2671
Size: 355 816 149 294 58 318 104 577
Cuts at: 2671 5187
Size: 2516
Fragments arranged by size:
2516 816 577 355 318 294 149 104
58
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3998 4030 4802 4834 5088 5120 5187
Size: 3998 32 772 32 254 32 67
Fragments arranged by size:
3998 772 254 67 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 268 1666 3106 4176 5187
Size: 268 1398 1440 1070 1011
Fragments arranged by size:
1440 1398 1070 1011 268
XmaI C'CCGG_G
Cuts at: 0 50 131 203 1059 1961 5187
Size: 50 81 72 856 902 3226
Fragments arranged by size:
3226 902 856 81 72 50
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AhdI AleI AloI
AlwNI ApaI ApoI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspMI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI CspCI DraIII DrdI EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FseI HaeII Hin4I HincII HindIII KasI KpnI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI
NruI NspI PasI PflMI PfoI PmlI PpuMI PshAI
PspOMI PstI PvuII SacI SacII SanDI SapI SfcI
SfiI SfoI SmaI SmlI SrfI StuI StyI TaqII
TstI XcmI XmaI
Enzymes that do not cut:
AatII AclI AflII AflIII AgeI AlfI ApaLI AscI
AseI AsiSI AvrII BaeI BciVI BclI BfrBI BsaBI
BsaWI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI
BsrGI BssHII BstZ17I ClaI DraI EagI EcoRV FspI
FspAI HpaI MfeI MluI NdeI NotI NsiI PacI
PciI PmeI PpiI PsiI PspXI PsrI PvuI RsrII
SalI SbfI ScaI SexAI SgfI SgrAI SnaBI SpeI
SphI SspI SwaI TatI Tth111I XbaI XhoI XmnI
ZraI