Restriction map for
pFN21AA1717
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# NO CUT ENZYMES ( 151 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI AspEI AspI AviII AvrII
BalI BamHI BbeI BbrPI BbsI
BbvCI BcgI BclI BfaI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaBI BsaHI BsaI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I Cac8I
CelII CfoI Cfr10I ClaI CpoI
DraI DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
FauI FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HindIII
HpaI KasI KspI MaeI MamI
MfeI MluI MluNI MroI MscI
MspA1I MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NspI PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacII
SbfI SfcI SfoI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tth111I Van91I XbaI
XhoI
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# ONE CUT ENZYMES ( 46 enzymes) #
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Acc65I 1055 GGTACC G^GTACC
AlwI 625 GATCC GGATC(4/5)
AlwNI 771 CAGGGACTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 1055 GGTACC G^GTACC
AspHI 988 GAGCTC G[AT]GC[AT]^C
AvaI 1024 CCCGGG C^[CT]CG[GA]G
BbvI 167 GCAGC GCAGC(8/12)
BciVI 850 GTATCC GTATCC(6/5)
BglI 1075 GCCTTCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsgI 75 CTGCAC GTGCAG(16/14)
BsiHKAI 988 GAGCTC G[AT]GC[AT]^C
BsmAI 306 GAGAC GTCTC(1/5)
BsoBI 1024 CCCGGG C^[CT]CG[GA]G
BstBI 743 TTCGAA TT^CGAA
BstYI 624 AGATCC [GA]^GATC[CT]
EcoT14I 869 CCTTGG C^C[AT][AT]GG
EcoT22I 951 ATGCAT ATGCA^T
Fnu4HI 167 GCAGC GC^[ACGT]GC
HapII 1025 CCGG C^CGG
HpaII 1025 CCGG C^CGG
ItaI 167 GCAGC GC^[ACGT]GC
KpnI 1055 GGTACC GGTAC^C
MaeIII 82 GTCAC ^GT[ACGT]AC
MflI 624 AGATCC [GA]^GATC[CT]
MseI 785 TTAA T^TAA
MslI 822 CATTGATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 1025 CCGG C^CGG
NlaIII 346 CATG CATG^
NsiI 951 ATGCAT ATGCA^T
NspV 743 TTCGAA TT^CGAA
PpuMI 708 GGGACCT [GA]G^G[AT]CC[CT]
SacI 988 GAGCTC GAGCT^C
SalI 585 GTCGAC G^TCGAC
ScaI 857 AGTACT AGT^ACT
SexAI 1058 ACCAGGT A^CC[AT]GGT
SfiI 1074 GGCCTTCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 743 TTCGAA TT^CGAA
SmaI 1024 CCCGGG CCC^GGG
StyI 869 CCTTGG C^C[AT][AT]GG
Tru9I 785 TTAA T^TAA
TseI 167 GCAGC G^C[AT]GC
Tsp45I 82 GTCAC ^GT[GC]AC
XcmI 927 CCACCCCCGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 624 AGATCC [GA]^GATC[CT]
XmaCI 1024 CCCGGG C^CCGGG
XmaI 1024 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 259 GTAGAC GT^[AC][GT]AC
585 GTCGAC
AcsI 472 AAATTT [GA]^AATT[CT]
561 AAATTC
ApaI 779 GGGCCC GGGCC^C
940 GGGCCC
ApoI 472 AAATTT [GA]^AATT[CT]
561 AAATTC
Asp700I 147 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1071 GAAGGCCTTC
BanI 41 GGCACC G^G[CT][GA]CC
1055 GGTACC
BcnI 1024 CCCGG CC^[GC]GG
1025 CCGGG
BsaAI 252 CACGTA [CT]AC^GT[GA]
847 CACGTA
BseRI 90 CTCCTC GAGGAG(10/8)
25 GAGGAG
BstF5I 954 CATCC GGATG(2/0)
192 GGATG
DpnII 312 GATC ^GATC
625 GATC
DraII 708 GGGACCT [GA]G^G[ACGT]CC[CT]
779 GGGCCCT
EcoO109I 708 GGGACCT [GA]G^G[ACGT]CC[CT]
779 GGGCCCT
FokI 954 CATCC GGATG(9/13)
192 GGATG
HincII 585 GTCGAC GT[CT]^[GA]AC
763 GTTGAC
HindII 585 GTCGAC GT[CT]^[GA]AC
763 GTTGAC
HinfI 634 GAATC G^A[ACGT]TC
658 GAATC
HphI 83 TCACC GGTGA(8/7)
992 TCACC
MaeII 253 ACGT A^CGT
848 ACGT
MboI 312 GATC ^GATC
625 GATC
MwoI 970 GCAGTGGAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1075 GCCTTCCAGGC
NciI 1024 CCCGG CC^[GC]GG
1025 CCGGG
NdeII 312 GATC ^GATC
625 GATC
SapI 985 GAAGAGC GCTCTTC(1/4)
613 GCTCTTC
Sau3AI 312 GATC ^GATC
625 GATC
StuI 535 AGGCCT AGG^CCT
1073 AGGCCT
TaqI 586 TCGA T^CGA
744 TCGA
TfiI 634 GAATC G^A[AT]TC
658 GAATC
TthHB8I 586 TCGA T^CGA
744 TCGA
XmnI 147 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1071 GAAGGCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AciI AfaI AluI AvaII BanII
BmyI BpmI BsaJI BsiYI BslI
BsmFI Bsp1286I BsrI BssKI BstNI
Cfr13I DdeI EarI EcoRII HaeIII
Ksp632I MboII MnlI MvaI NlaIV
RsaI Sau96I ScrFI SfaNI Tsp509I
TspRI