(Circular) (Six-base) MAPSORT of: pFN21AA1717.seq Check: 9360 from: 1 to: 5803 pFN21AA1717 5803 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3613 278 Size: 53 83 186 936 2077 2468 Fragments arranged by size: 2468 2077 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2265 2591 3146 1937 Size: 328 326 555 4594 Fragments arranged by size: 4594 555 328 326 Acc65I G'GTAC_C Cuts at: 3060 3124 3060 Size: 64 5739 AclI AA'CG_TT Cuts at: 1563 3930 4303 1563 Size: 2367 373 3063 Fragments arranged by size: 3063 2367 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2550 3066 3095 3866 5250 5518 1632 Size: 918 516 29 771 1384 268 1917 Fragments arranged by size: 1917 1384 918 771 516 268 29 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5606 AflIII A'CryG_T Cuts at: 1203 4702 5679 1203 Size: 3499 977 1327 Fragments arranged by size: 3499 1327 977 AgeI A'CCGG_T Cuts at: 4634 4634 Size: 5803 AhdI GACnn_n'nnGTC Cuts at: 4532 4532 Size: 5803 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5803 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5803 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2789 2821 2789 Size: 32 5771 AlwNI CAG_nnn'CTG Cuts at: 2781 3546 5118 2781 Size: 765 1572 3466 Fragments arranged by size: 3466 1572 765 ApaI G_GGCC'C Cuts at: 2788 2949 2788 Size: 161 5642 ApaLI G'TGCA_C Cuts at: 3860 5016 3860 Size: 1156 4647 ApoI r'AATT_y Cuts at: 1493 2477 2566 3112 3338 3490 5599 1493 Size: 984 89 546 226 152 2109 1697 Fragments arranged by size: 2109 1697 984 546 226 152 89 AseI AT'TA_AT Cuts at: 160 1032 4357 160 Size: 872 3325 1606 Fragments arranged by size: 3325 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5803 AvaI C'yCGr_G Cuts at: 1870 1943 3029 3128 1870 Size: 73 1086 99 4545 Fragments arranged by size: 4545 1086 99 73 BamHI G'GATC_C Cuts at: 3133 3500 3133 Size: 367 5436 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2046 3060 3124 4579 618 Size: 325 217 627 259 1014 64 1455 1842 Fragments arranged by size: 1842 1455 1014 627 325 259 217 64 BanII G_rGCy'C Cuts at: 1391 2788 2844 2949 2997 3122 1391 Size: 1397 56 105 48 125 4072 Fragments arranged by size: 4072 1397 125 105 56 48 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4832 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3994 4028 1903 Size: 34 2057 34 3678 Fragments arranged by size: 3678 2057 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2866 3695 4905 2866 Size: 829 1210 3764 Fragments arranged by size: 3764 1210 829 BclI T'GATC_A Cuts at: 1548 1548 Size: 5803 BfrBI ATG'CAT Cuts at: 2958 2958 Size: 5803 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3086 4414 136 Size: 107 122 71 631 605 1414 1328 1525 Fragments arranged by size: 1525 1414 1328 631 605 122 107 71 BglII A'GATC_T Cuts at: 5798 5798 Size: 5803 BlpI GC'TnA_GC Cuts at: 3220 3220 Size: 5803 Bme1580I G_kGCm'C Cuts at: 1790 2049 2258 2788 2949 3864 5020 1790 Size: 259 209 530 161 915 1156 2573 Fragments arranged by size: 2573 1156 915 530 259 209 161 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5803 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2791 3534 4492 Size: 416 53 83 399 24 1374 743 958 Cuts at: 4492 442 Size: 1753 Fragments arranged by size: 1753 1374 958 743 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5803 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2201 2234 2445 2703 4463 1239 Size: 962 33 211 258 1760 2579 Fragments arranged by size: 2579 1760 962 258 211 33 Bpu10I CC'TnA_GC Cuts at: 2838 2838 Size: 5803 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3928 4793 5091 3928 Size: 865 298 4640 Fragments arranged by size: 4640 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4466 882 Size: 617 2967 2219 Fragments arranged by size: 2967 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2259 2854 3594 493 Size: 711 1055 595 740 2702 Fragments arranged by size: 2702 1055 740 711 595 BsaBI GATnn'nnATC Cuts at: 5797 5797 Size: 5803 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3610 3992 4627 275 Size: 53 83 186 936 2077 382 635 1451 Fragments arranged by size: 2077 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4236 4634 4908 5055 5466 4236 Size: 398 274 147 411 4573 Fragments arranged by size: 4573 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2789 2819 2855 2885 1178 Size: 30 165 30 1386 30 36 30 4096 Fragments arranged by size: 4096 1386 165 36 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2045 2086 1186 Size: 859 41 4903 Fragments arranged by size: 4903 859 41 BseYI C'CCAG_C Cuts at: 3093 5006 5502 3093 Size: 1913 496 3394 Fragments arranged by size: 3394 1913 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2065 1163 Size: 573 329 4901 Fragments arranged by size: 4901 573 329 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3253 4014 5042 1540 Size: 275 186 1252 761 1028 2301 Fragments arranged by size: 2301 1252 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2997 3864 3949 5020 2997 Size: 867 85 1071 3780 Fragments arranged by size: 3780 1071 867 85 BsmI GAATG_Cn' Cuts at: 3314 3407 3314 Size: 93 5710 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2049 2258 2788 2844 2949 2997 3122 Size: 399 259 209 530 56 105 48 125 Cuts at: 3122 3864 3949 5020 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 530 399 259 209 125 105 85 56 48 BspEI T'CCGG_A Cuts at: 5466 5466 Size: 5803 BspHI T'CATG_A Cuts at: 3692 5422 3692 Size: 1730 4073 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3158 844 Size: 2314 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3690 1394 Size: 429 1867 3507 Fragments arranged by size: 3507 1867 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4298 4472 65 Size: 1183 3050 174 1396 Fragments arranged by size: 3050 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4447 4634 1700 Size: 110 2637 187 2869 Fragments arranged by size: 2869 2637 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5803 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5803 BssSI C'ACGA_G Cuts at: 3863 4875 3863 Size: 1012 4791 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5803 BstBI TT'CG_AA Cuts at: 2749 4607 2749 Size: 1858 3945 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5803 BstYI r'GATC_y Cuts at: 1972 2629 3133 3500 3885 3902 5354 5440 5452 Size: 657 504 367 385 17 1452 86 12 Cuts at: 5452 5798 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 657 504 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4312 Fragments arranged by size: 4312 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5064 Fragments arranged by size: 5064 683 56 BtsI GCAGTG_nn' Cuts at: 2982 3319 4113 4133 2982 Size: 337 794 20 4652 Fragments arranged by size: 4652 794 337 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5803 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5768 DraI TTT'AAA Cuts at: 3107 3107 Size: 5803 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4810 809 Size: 507 3494 1802 Fragments arranged by size: 3494 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3250 4139 5768 Size: 54 1425 50 275 18 1420 889 1629 Cuts at: 5768 8 Size: 43 Fragments arranged by size: 1629 1425 1420 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3250 1537 Size: 275 1438 4090 Fragments arranged by size: 4090 1438 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2625 2985 3029 3733 1353 Size: 1272 360 44 704 3423 Fragments arranged by size: 3423 1272 704 360 44 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4390 4764 4910 387 Size: 1064 138 2801 374 146 1280 Fragments arranged by size: 2801 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2995 2995 Size: 5803 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2201 2234 2445 2550 2703 3066 3095 Size: 393 569 33 211 105 153 363 29 Cuts at: 3095 3866 4463 5250 5518 1239 Size: 771 597 787 268 1524 Fragments arranged by size: 1524 787 771 597 569 393 363 268 211 153 105 33 29 EcoO109I rG'GnC_Cy Cuts at: 2714 2785 2714 Size: 71 5732 EcoRI G'AATT_C Cuts at: 3112 3490 3112 Size: 378 5425 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2176 2176 Size: 5803 FspI TGC'GCA Cuts at: 4309 4309 Size: 5803 HaeII r_GCGC'y Cuts at: 1678 4950 1678 Size: 3272 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4444 4476 4518 4550 1373 Size: 32 135 32 2872 32 42 32 2626 Fragments arranged by size: 2872 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2592 2770 3147 3393 669 Size: 988 281 654 178 377 246 3079 Fragments arranged by size: 3079 988 654 377 281 246 178 HindIII A'AGCT_T Cuts at: 748 748 Size: 5803 HpaI GTT'AAC Cuts at: 3393 3393 Size: 5803 KpnI G_GTAC'C Cuts at: 3064 3128 3064 Size: 64 5739 MfeI C'AATT_G Cuts at: 3402 3402 Size: 5803 MluI A'CGCG_T Cuts at: 5679 5679 Size: 5803 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4917 5101 1305 Size: 3612 184 2007 Fragments arranged by size: 3612 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3981 Fragments arranged by size: 3981 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2831 3761 4120 4279 518 Size: 648 1665 930 359 159 2042 Fragments arranged by size: 2042 1665 930 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3219 3896 5044 5289 1606 Size: 1613 677 1148 245 2120 Fragments arranged by size: 2120 1613 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4312 Fragments arranged by size: 4312 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5803 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5803 NotI GC'GGCC_GC Cuts at: 3250 3250 Size: 5803 NsiI A_TGCA'T Cuts at: 2960 2960 Size: 5803 NspI r_CATG'y Cuts at: 1132 1229 3161 4706 1132 Size: 97 1932 1545 2229 Fragments arranged by size: 2229 1932 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5803 PciI A'CATG_T Cuts at: 4702 4702 Size: 5803 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5803 PmeI GTTT'AAAC Cuts at: 3107 3107 Size: 5803 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2789 2821 3872 3904 5385 5417 2789 Size: 32 1051 32 1481 32 3175 Fragments arranged by size: 3175 1481 1051 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2714 2714 Size: 5803 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5803 PsiI TTA'TAA Cuts at: 2739 3373 2739 Size: 634 5169 PspOMI G'GGCC_C Cuts at: 2784 2945 2784 Size: 161 5642 PstI C_TGCA'G Cuts at: 830 3155 830 Size: 2325 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5803 SacI G_AGCT'C Cuts at: 2997 2997 Size: 5803 SalI G'TCGA_C Cuts at: 1936 2590 3145 1936 Size: 654 555 4594 Fragments arranged by size: 4594 654 555 SapI GCTCTTCn'nnn_ Cuts at: 2625 2985 2625 Size: 360 5443 SbfI CC_TGCA'GG Cuts at: 3155 3155 Size: 5803 ScaI AGT'ACT Cuts at: 2864 4051 5763 2864 Size: 1187 1712 2904 Fragments arranged by size: 2904 1712 1187 SexAI A'CCwGG_T Cuts at: 3063 3063 Size: 5803 SfcI C'TryA_G Cuts at: 826 1045 3151 4286 4967 5158 826 Size: 219 2106 1135 681 191 1471 Fragments arranged by size: 2106 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 3086 3086 Size: 5803 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5803 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5803 SmaI CCC'GGG Cuts at: 1872 3031 3130 1872 Size: 1159 99 4545 Fragments arranged by size: 4545 1159 99 SmlI C'TyrA_G Cuts at: 820 1017 1943 3907 4808 5070 820 Size: 197 926 1964 901 262 1553 Fragments arranged by size: 1964 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3594 493 Size: 3101 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5803 SphI G_CATG'C Cuts at: 1132 3161 1132 Size: 2029 3774 SspI AAT'ATT Cuts at: 5 52 3727 5 Size: 47 3675 2081 Fragments arranged by size: 3675 2081 47 StuI AGG'CCT Cuts at: 1517 2542 3080 1517 Size: 1025 538 4240 Fragments arranged by size: 4240 1025 538 StyI C'CwwG_G Cuts at: 513 1065 2004 2874 3242 513 Size: 552 939 870 368 3074 Fragments arranged by size: 3074 939 870 552 368 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3107 3830 3847 4000 4185 4630 325 Size: 1255 1527 723 17 153 185 445 1498 Fragments arranged by size: 1527 1498 1255 723 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2862 4049 5761 Size: 274 80 33 51 1443 885 1187 1712 Cuts at: 5761 96 Size: 138 Fragments arranged by size: 1712 1443 1187 885 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2556 2588 5763 5795 2556 Size: 32 3175 32 2564 Fragments arranged by size: 3175 2564 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5803 XbaI T'CTAG_A Cuts at: 3139 3139 Size: 5803 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2939 2939 Size: 5803 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5803 XmaI C'CCGG_G Cuts at: 1870 3029 3128 1870 Size: 1159 99 4545 Fragments arranged by size: 4545 1159 99 XmnI GAAnn'nnTTC Cuts at: 2156 3080 3932 2156 Size: 924 852 4027 Fragments arranged by size: 4027 924 852 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3611 276 Size: 53 83 186 936 2077 2468 Fragments arranged by size: 2468 2077 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacII SanDI SfoI SrfI SwaI