Restriction map for
pFN21AA1682
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# NO CUT ENZYMES ( 122 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflIII AgeI AhdI
AscI Asp700I Asp718I AspEI AspI
AvaI AviII BamHI BbeI BbrPI
BcgI BglII BlpI Bpu1102I BsaBI
BsaHI BsaI BsaWI BseAI BsiEI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstXI BstYI
BstZ17I CelII Cfr10I ClaI CpoI
EagI Eam1105I EclXI Eco52I EcoNI
EcoO65I EcoRV FseI FspI HgaI
Hin1I HpaI KasI KpnI KspI
MamI MfeI MflI MluI MroI
MslI MunI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NspI NspV PaeR7I PflFI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SexAI SfiI
SfoI SfuI SgrAI SmaI SpeI
SphI SspBI SspI StuI SwaI
Tth111I XbaI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AflII 622 CTTAAG C^TTAAG
Alw44I 1387 GTGCAC G^TGCAC
AocI 76 CCTGAGG CC^T[ACGT]AGG
ApaLI 1387 GTGCAC G^TGCAC
AseI 1795 ATTAAT AT^TAAT
AsnI 1795 ATTAAT AT^TAAT
BanI 34 GGCACC G^G[CT][GA]CC
BciVI 1527 GGATAC GTATCC(6/5)
BclI 342 TGATCA T^GATCA
BcnI 1401 CCCGG CC^[GC]GG
BfrI 622 CTTAAG C^TTAAG
BglI 2059 GCCCCCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 2131 CTCCAG CTGGAG(16/14)
BsaAI 67 TACGTA [CT]AC^GT[GA]
BsrDI 1601 CATTGC GCAATG(2/0)
BstAPI 1380 GCAGCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 76 CCTGAGG CC^T[ACGT]AGG
DraI 1000 TTTAAA TTT^AAA
DraIII 1557 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1431 GACTGTTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1869 CCATGG C^C[GA][CT]GG
Eco81I 76 CCTGAGG CC^T[ACGT]AGG
EcoRI 1411 GAATTC G^AATTC
EcoT22I 1178 ATGCAT ATGCA^T
FauI 2099 CCCGC CCCGC(4/6)
FbaI 342 TGATCA T^GATCA
HapII 1402 CCGG C^CGG
HincII 685 GTCAAC GT[CT]^[GA]AC
HindII 685 GTCAAC GT[CT]^[GA]AC
HindIII 2177 AAGCTT A^AGCTT
HpaII 1402 CCGG C^CGG
MspI 1402 CCGG C^CGG
NciI 1401 CCCGG CC^[GC]GG
NcoI 1869 CCATGG C^CATGG
NsiI 1178 ATGCAT ATGCA^T
PacI 1756 TTAATTAA TTAAT^TAA
PflMI 2165 CCAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 1795 ATTAAT AT^TAAT
SacI 1810 GAGCTC GAGCT^C
SbfI 969 CCTGCAGG CCTGCA^GG
SmlI 622 CTTAAG C^T[CT][GA]AG
SnaBI 67 TACGTA TAC^GTA
Sse8387I 969 CCTGCAGG CCTGCA^GG
Tsp45I 583 GTCAC ^GT[GC]AC
Van91I 2165 CCAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 21 enzymes) #
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AlwI 589 GATCC GGATC(4/5)
1305 GGATC
AlwNI 581 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG
2166 CAGTGGCTG
Aor51HI 764 AGCGCT AGC^GCT
1922 AGCGCT
ApaI 1897 GGGCCC GGGCC^C
1916 GGGCCC
AspHI 1387 GTGCAC G[AT]GC[AT]^C
1810 GAGCTC
AvrII 1956 CCTAGG C^CTAGG
2229 CCTAGG
BbsI 943 GAAGAC GAAGAC(2/6)
2191 GAAGAC
BbvCI 2068 GCTGAGG CCTCAGC(-5/-2)
2085 GCTGAGG
BlnI 1956 CCTAGG C^CTAGG
2229 CCTAGG
BpuAI 943 GAAGAC GAAGAC(2/6)
2191 GAAGAC
BseRI 1171 GAGGAG GAGGAG(10/8)
1339 GAGGAG
BsiHKAI 1387 GTGCAC G[AT]GC[AT]^C
1810 GAGCTC
BsiWI 65 CGTACG C^GTACG
69 CGTACG
BsmI 1176 GAATGC GAATGC(1/-1)
1661 GAATGC
Eco47III 764 AGCGCT AGC^GCT
1922 AGCGCT
HaeII 764 AGCGCT [GA]GCGC^[CT]
1922 AGCGCT
PstI 970 CTGCAG CTGCA^G
1236 CTGCAG
ScaI 171 AGTACT AGT^ACT
1027 AGTACT
SfaNI 1180 GCATC GCATC(5/9)
1395 GCATC
SfcI 970 CTGCAG C^T[GA][CT]AG
1236 CTGCAG
XcmI 787 CCAATATGGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1950 CCAAATCCTAGGTGG
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# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AvaII BalI BanII BbvI BfaI
BmyI BsaJI BsgI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII DraII EaeI EarI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HhaI HinP1I HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MseI MspA1I MvaI MwoI NdeII
NlaIII NlaIV PvuII RsaI Sau3AI
Sau96I ScrFI StyI TaqI TfiI
Tru9I TseI Tsp509I TspRI TthHB8I