(Circular) (Six-base) MAPSORT of: pFN21AA1682.seq Check: 488 from: 1 to: 6946 pFN21AA1682 6946 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4756 278 Size: 53 83 186 936 3220 2468 Fragments arranged by size: 3220 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4289 1937 Size: 2352 4594 Acc65I G'GTAC_C Cuts at: 4267 4267 Size: 6946 AclI AA'CG_TT Cuts at: 1563 5073 5446 1563 Size: 3510 373 3063 Fragments arranged by size: 3510 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2201 2420 2775 5009 6393 6661 1632 Size: 569 219 355 2234 1384 268 1917 Fragments arranged by size: 2234 1917 1384 569 355 268 219 AfeI AGC'GCT Cuts at: 2771 3929 2771 Size: 1158 5788 AflII C'TTAA_G Cuts at: 820 1017 2627 820 Size: 197 1610 5139 Fragments arranged by size: 5139 1610 197 AflIII A'CryG_T Cuts at: 1203 5845 6822 1203 Size: 4642 977 1327 Fragments arranged by size: 4642 1327 977 AgeI A'CCGG_T Cuts at: 5777 5777 Size: 6946 AhdI GACnn_n'nnGTC Cuts at: 5675 5675 Size: 6946 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6946 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6946 AlwNI CAG_nnn'CTG Cuts at: 2591 4176 4689 6261 2591 Size: 1585 513 1572 3276 Fragments arranged by size: 3276 1585 1572 513 ApaI G_GGCC'C Cuts at: 3906 3925 3906 Size: 19 6927 ApaLI G'TGCA_C Cuts at: 3392 5003 6159 3392 Size: 1611 1156 4179 Fragments arranged by size: 4179 1611 1156 ApoI r'AATT_y Cuts at: 1493 2088 2901 3002 3038 3416 4255 4481 4633 Size: 595 813 101 36 378 839 226 152 Cuts at: 4633 6742 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 839 813 595 378 226 152 101 36 AseI AT'TA_AT Cuts at: 160 1032 3801 5500 160 Size: 872 2769 1699 1606 Fragments arranged by size: 2769 1699 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6946 AvaI C'yCGr_G Cuts at: 1870 1943 4271 1870 Size: 73 2328 4545 Fragments arranged by size: 4545 2328 73 AvrII C'CTAG_G Cuts at: 3961 4234 3961 Size: 273 6673 BamHI G'GATC_C Cuts at: 4276 4643 4276 Size: 367 6579 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2039 4267 5722 618 Size: 325 217 627 252 2228 1455 1842 Fragments arranged by size: 2228 1842 1455 627 325 252 217 BanII G_rGCy'C Cuts at: 1391 3819 3906 3925 4265 1391 Size: 2428 87 19 340 4072 Fragments arranged by size: 4072 2428 340 87 19 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2955 4203 928 Size: 971 1056 1248 3671 Fragments arranged by size: 3671 1248 1056 971 BbvCI CC'TCA_GC Cuts at: 4074 4091 4074 Size: 17 6929 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5137 5171 1903 Size: 34 50 34 3116 34 3678 Fragments arranged by size: 3678 3116 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3526 4838 6048 3526 Size: 1312 1210 4424 Fragments arranged by size: 4424 1312 1210 BclI T'GATC_A Cuts at: 1548 2347 1548 Size: 799 6147 BfrBI ATG'CAT Cuts at: 3185 3185 Size: 6946 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4070 5557 136 Size: 107 122 71 631 605 2398 1487 1525 Fragments arranged by size: 2398 1525 1487 631 605 122 107 71 BglII A'GATC_T Cuts at: 6941 6941 Size: 6946 BlpI GC'TnA_GC Cuts at: 4363 4363 Size: 6946 Bme1580I G_kGCm'C Cuts at: 1790 2827 3396 3906 3925 5007 6163 1790 Size: 1037 569 510 19 1082 1156 2573 Fragments arranged by size: 2573 1156 1082 1037 569 510 19 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6946 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4677 5635 442 Size: 416 53 83 399 24 3260 958 1753 Fragments arranged by size: 3260 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6946 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4121 5606 1239 Size: 2882 1485 2579 Fragments arranged by size: 2882 2579 1485 Bpu10I CC'TnA_GC Cuts at: 4074 4091 4074 Size: 17 6929 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5071 5936 6234 5071 Size: 865 298 5783 Fragments arranged by size: 5783 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5609 882 Size: 617 4110 2219 Fragments arranged by size: 4110 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2074 4737 493 Size: 711 870 2663 2702 Fragments arranged by size: 2702 2663 870 711 BsaBI GATnn'nnATC Cuts at: 6940 6940 Size: 6946 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4753 5135 5770 275 Size: 53 83 186 936 3220 382 635 1451 Fragments arranged by size: 3220 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5379 5777 6051 6198 6609 5379 Size: 398 274 147 411 5716 Fragments arranged by size: 5716 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2509 2539 1178 Size: 30 165 30 1106 30 5585 Fragments arranged by size: 5585 1106 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3191 3359 1186 Size: 2005 168 4773 Fragments arranged by size: 4773 2005 168 BseYI C'CCAG_C Cuts at: 2008 3924 6149 6645 2008 Size: 1916 2225 496 2309 Fragments arranged by size: 2309 2225 1916 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2218 2556 2786 3602 1163 Size: 573 482 338 230 816 4507 Fragments arranged by size: 4507 816 573 482 338 230 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4396 5157 6185 1540 Size: 275 186 2395 761 1028 2301 Fragments arranged by size: 2395 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3396 3819 5007 5092 6163 3396 Size: 423 1188 85 1071 4179 Fragments arranged by size: 4179 1188 1071 423 85 BsiWI C'GTAC_G Cuts at: 2070 2074 2070 Size: 4 6942 BsmI GAATG_Cn' Cuts at: 3187 3672 4457 4550 3187 Size: 485 785 93 5583 Fragments arranged by size: 5583 785 485 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2827 3396 3819 3906 3925 4265 5007 Size: 399 1037 569 423 87 19 340 742 Cuts at: 5007 5092 6163 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 1037 742 569 423 399 340 87 85 19 BspEI T'CCGG_A Cuts at: 6609 6609 Size: 6946 BspHI T'CATG_A Cuts at: 4835 6565 4835 Size: 1730 5216 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4301 844 Size: 3457 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4833 1394 Size: 429 3010 3507 Fragments arranged by size: 3507 3010 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3605 5441 5615 65 Size: 1183 2357 1836 174 1396 Fragments arranged by size: 2357 1836 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5590 5777 1700 Size: 110 3780 187 2869 Fragments arranged by size: 3780 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6946 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6946 BssSI C'ACGA_G Cuts at: 5006 6018 5006 Size: 1012 5934 BstAPI GCAn_nnn'nTGC Cuts at: 794 3391 794 Size: 2597 4349 BstBI TT'CG_AA Cuts at: 5750 5750 Size: 6946 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6946 BstYI r'GATC_y Cuts at: 1972 4276 4643 5028 5045 6497 6583 6595 6941 Size: 2304 367 385 17 1452 86 12 346 Cuts at: 6941 1972 Size: 1977 Fragments arranged by size: 2304 1977 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2082 2082 Size: 6946 BtgI C'CryG_G Cuts at: 513 1065 3874 513 Size: 552 2809 3585 Fragments arranged by size: 3585 2809 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6207 Fragments arranged by size: 6207 683 56 BtsI GCAGTG_nn' Cuts at: 2428 2688 2843 3238 4462 5256 5276 2428 Size: 260 155 395 1224 794 20 4098 Fragments arranged by size: 4098 1224 794 395 260 155 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6946 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3263 3298 529 Size: 35 2699 35 4177 Fragments arranged by size: 4177 2699 35 35 DraI TTT'AAA Cuts at: 3007 4250 3007 Size: 1243 5703 DraIII CAC_nnn'GTG Cuts at: 3567 3567 Size: 6946 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3442 5953 809 Size: 507 2126 2511 1802 Fragments arranged by size: 2511 2126 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3295 3970 3978 Size: 54 1425 50 275 18 1465 675 8 Cuts at: 3978 4206 4393 5282 6911 8 Size: 228 187 889 1629 43 Fragments arranged by size: 1629 1465 1425 889 675 275 228 187 54 50 43 18 8 EagI C'GGCC_G Cuts at: 1537 1812 4393 1537 Size: 275 2581 4090 Fragments arranged by size: 4090 2581 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2136 2472 2751 2754 4876 1353 Size: 783 336 279 3 2122 3423 Fragments arranged by size: 3423 2122 783 336 279 3 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3317 5533 5907 6053 387 Size: 1064 138 1728 2216 374 146 1280 Fragments arranged by size: 2216 1728 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3817 3817 Size: 6946 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2201 2420 2775 4121 5009 5606 6393 Size: 393 569 219 355 1346 888 597 787 Cuts at: 6393 6661 1239 Size: 268 1524 Fragments arranged by size: 1524 1346 888 787 597 569 393 355 268 219 EcoO109I rG'GnC_Cy Cuts at: 2047 3902 4063 4078 2047 Size: 1855 161 15 4915 Fragments arranged by size: 4915 1855 161 15 EcoRI G'AATT_C Cuts at: 3416 4255 4633 3416 Size: 839 378 5729 Fragments arranged by size: 5729 839 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2077 2147 2269 2908 3564 4177 2077 Size: 70 122 639 656 613 4846 Fragments arranged by size: 4846 656 639 613 122 70 FspI TGC'GCA Cuts at: 5452 5452 Size: 6946 HaeII r_GCGC'y Cuts at: 1678 2773 3931 6093 1678 Size: 1095 1158 2162 2531 Fragments arranged by size: 2531 2162 1158 1095 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2255 2287 3170 3202 5587 Size: 32 135 32 683 32 883 32 2385 Cuts at: 5587 5619 5661 5693 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2385 883 683 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2692 4290 4536 669 Size: 988 281 754 1598 246 3079 Fragments arranged by size: 3079 1598 988 754 281 246 HindIII A'AGCT_T Cuts at: 748 4182 748 Size: 3434 3512 HpaI GTT'AAC Cuts at: 4536 4536 Size: 6946 KpnI G_GTAC'C Cuts at: 4271 4271 Size: 6946 MfeI C'AATT_G Cuts at: 4545 4545 Size: 6946 MluI A'CGCG_T Cuts at: 6822 6822 Size: 6946 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3054 3213 3920 6060 6244 1305 Size: 1749 159 707 2140 184 2007 Fragments arranged by size: 2140 2007 1749 707 184 159 MscI TGG'CCA Cuts at: 10 64 1489 1832 3297 3972 3980 4208 10 Size: 54 1425 343 1465 675 8 228 2748 Fragments arranged by size: 2748 1465 1425 675 343 228 54 8 MslI CAynn'nnrTG Cuts at: 518 1166 4904 5263 5422 518 Size: 648 3738 359 159 2042 Fragments arranged by size: 3738 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2155 3087 3388 3849 4362 5039 6187 6432 Size: 549 932 301 461 513 677 1148 245 Cuts at: 6432 1606 Size: 2120 Fragments arranged by size: 2120 1148 932 677 549 513 461 301 245 NcoI C'CATG_G Cuts at: 513 1065 3874 513 Size: 552 2809 3585 Fragments arranged by size: 3585 2809 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6946 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6946 NotI GC'GGCC_GC Cuts at: 4393 4393 Size: 6946 NsiI A_TGCA'T Cuts at: 3187 3187 Size: 6946 NspI r_CATG'y Cuts at: 1132 1229 4304 5849 1132 Size: 97 3075 1545 2229 Fragments arranged by size: 3075 2229 1545 97 PacI TTA_AT'TAA Cuts at: 3765 3765 Size: 6946 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6946 PciI A'CATG_T Cuts at: 5845 5845 Size: 6946 PflMI CCAn_nnn'nTGG Cuts at: 1108 4176 1108 Size: 3068 3878 PfoI T'CCnGG_A Cuts at: 2190 2190 Size: 6946 PmeI GTTT'AAAC Cuts at: 4250 4250 Size: 6946 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5015 5047 6528 6560 5015 Size: 32 1481 32 5401 Fragments arranged by size: 5401 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6946 PsiI TTA'TAA Cuts at: 4516 4516 Size: 6946 PspOMI G'GGCC_C Cuts at: 3902 3921 3902 Size: 19 6927 PstI C_TGCA'G Cuts at: 830 2979 3245 4298 830 Size: 2149 266 1053 3478 Fragments arranged by size: 3478 2149 1053 266 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6946 PvuII CAG'CTG Cuts at: 2155 3087 3388 3849 2155 Size: 932 301 461 5252 Fragments arranged by size: 5252 932 461 301 SacI G_AGCT'C Cuts at: 3819 3819 Size: 6946 SalI G'TCGA_C Cuts at: 1936 4288 1936 Size: 2352 4594 SbfI CC_TGCA'GG Cuts at: 2979 4298 2979 Size: 1319 5627 ScaI AGT'ACT Cuts at: 2178 3034 5194 6906 2178 Size: 856 2160 1712 2218 Fragments arranged by size: 2218 2160 1712 856 SfcI C'TryA_G Cuts at: 826 1045 2975 3241 4294 5429 6110 6301 826 Size: 219 1930 266 1053 1135 681 191 1471 Fragments arranged by size: 1930 1471 1135 1053 681 266 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6946 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6946 SmaI CCC'GGG Cuts at: 1872 4273 1872 Size: 2401 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2627 5050 5951 6213 820 Size: 197 926 684 2423 901 262 1553 Fragments arranged by size: 2423 1553 926 901 684 262 197 SnaBI TAC'GTA Cuts at: 493 2074 4737 493 Size: 1581 2663 2702 Fragments arranged by size: 2702 2663 1581 SpeI A'CTAG_T Cuts at: 152 152 Size: 6946 SphI G_CATG'C Cuts at: 1132 4304 1132 Size: 3172 3774 SspI AAT'ATT Cuts at: 5 52 4870 5 Size: 47 4818 2081 Fragments arranged by size: 4818 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6946 StyI C'CwwG_G Cuts at: 513 1065 2043 3874 3961 4234 4385 513 Size: 552 978 1831 87 273 151 3074 Fragments arranged by size: 3074 1831 978 552 273 151 87 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2249 3281 4973 4990 5143 5328 5773 Size: 1255 669 1032 1692 17 153 185 445 Cuts at: 5773 325 Size: 1498 Fragments arranged by size: 1692 1498 1255 1032 669 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2176 2673 3032 Size: 274 80 33 51 1443 199 497 359 Cuts at: 3032 5192 6904 96 Size: 2160 1712 138 Fragments arranged by size: 2160 1712 1443 497 359 274 199 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2485 2517 2734 2766 6906 6938 2485 Size: 32 217 32 4140 32 2493 Fragments arranged by size: 4140 2493 217 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6946 XbaI T'CTAG_A Cuts at: 4282 4282 Size: 6946 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2799 3962 2799 Size: 1163 5783 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6946 XmaI C'CCGG_G Cuts at: 1870 4271 1870 Size: 2401 4545 XmnI GAAnn'nnTTC Cuts at: 5075 5075 Size: 6946 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4754 276 Size: 53 83 186 936 3220 2468 Fragments arranged by size: 3220 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI BaeI BbeI BplI BsmBI BstXI BstZ17I EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI