Restriction map for
pFN21AA1682
(Linear) (Six-base) MAPSORT of: KIAA1682.seq Check: 5148 from: 1 to: 2241
KIAA1682.orf
With 182 enzymes: SgfI *
October 24, 2006 15:09 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 415 770 2241
Size: 196 219 355 1471
Fragments arranged by size:
1471 355 219 196
AfeI AGC'GCT
Cuts at: 0 766 1924 2241
Size: 766 1158 317
Fragments arranged by size:
1158 766 317
AflII C'TTAA_G
Cuts at: 0 622 2241
Size: 622 1619
AlwNI CAG_nnn'CTG
Cuts at: 0 586 2171 2241
Size: 586 1585 70
Fragments arranged by size:
1585 586 70
ApaI G_GGCC'C
Cuts at: 0 1901 1920 2241
Size: 1901 19 321
Fragments arranged by size:
1901 321 19
ApaLI G'TGCA_C
Cuts at: 0 1387 2241
Size: 1387 854
ApoI r'AATT_y
Cuts at: 0 83 896 997 1033 1411 2241
Size: 83 813 101 36 378 830
Fragments arranged by size:
830 813 378 101 83 36
AseI AT'TA_AT
Cuts at: 0 1796 2241
Size: 1796 445
AvrII C'CTAG_G
Cuts at: 0 1956 2229 2241
Size: 1956 273 12
Fragments arranged by size:
1956 273 12
BanI G'GyrC_C
Cuts at: 0 34 2241
Size: 34 2207
BanII G_rGCy'C
Cuts at: 0 1814 1901 1920 2241
Size: 1814 87 19 321
Fragments arranged by size:
1814 321 87 19
BbsI GAAGACnn'nnnn_
Cuts at: 0 950 2198 2241
Size: 950 1248 43
Fragments arranged by size:
1248 950 43
BbvCI CC'TCA_GC
Cuts at: 0 2069 2086 2241
Size: 2069 17 155
Fragments arranged by size:
2069 155 17
BciVI GTATCCnnnnn_n'
Cuts at: 0 1521 2241
Size: 1521 720
BclI T'GATC_A
Cuts at: 0 342 2241
Size: 342 1899
BfrBI ATG'CAT
Cuts at: 0 1180 2241
Size: 1180 1061
BglI GCCn_nnn'nGGC
Cuts at: 0 2065 2241
Size: 2065 176
Bme1580I G_kGCm'C
Cuts at: 0 822 1391 1901 1920 2241
Size: 822 569 510 19 321
Fragments arranged by size:
822 569 510 321 19
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2116 2241
Size: 2116 125
Bpu10I CC'TnA_GC
Cuts at: 0 2069 2086 2241
Size: 2069 17 155
Fragments arranged by size:
2069 155 17
BsaAI yAC'GTr
Cuts at: 0 69 2241
Size: 69 2172
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 504 534 2241
Size: 504 30 1707
Fragments arranged by size:
1707 504 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1186 1354 2241
Size: 1186 168 887
Fragments arranged by size:
1186 887 168
BseYI C'CCAG_C
Cuts at: 0 3 1919 2241
Size: 3 1916 322
Fragments arranged by size:
1916 322 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 213 551 781 1597 2241
Size: 213 338 230 816 644
Fragments arranged by size:
816 644 338 230 213
BsiHKAI G_wGCw'C
Cuts at: 0 1391 1814 2241
Size: 1391 423 427
Fragments arranged by size:
1391 427 423
BsiWI C'GTAC_G
Cuts at: 0 65 69 2241
Size: 65 4 2172
Fragments arranged by size:
2172 65 4
BsmI GAATG_Cn'
Cuts at: 0 1182 1667 2241
Size: 1182 485 574
Fragments arranged by size:
1182 574 485
Bsp1286I G_dGCh'C
Cuts at: 0 822 1391 1814 1901 1920 2241
Size: 822 569 423 87 19 321
Fragments arranged by size:
822 569 423 321 87 19
BsrDI GCAATG_nn'
Cuts at: 0 1600 2241
Size: 1600 641
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1386 2241
Size: 1386 855
Bsu36I CC'TnA_GG
Cuts at: 0 77 2241
Size: 77 2164
BtgI C'CryG_G
Cuts at: 0 1869 2241
Size: 1869 372
BtsI GCAGTG_nn'
Cuts at: 0 423 683 838 1233 2241
Size: 423 260 155 395 1008
Fragments arranged by size:
1008 423 395 260 155
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1258 1293 2241
Size: 1258 35 948
Fragments arranged by size:
1258 948 35
DraI TTT'AAA
Cuts at: 0 1002 2241
Size: 1002 1239
DraIII CAC_nnn'GTG
Cuts at: 0 1562 2241
Size: 1562 679
DrdI GACnn_nn'nnGTC
Cuts at: 0 1437 2241
Size: 1437 804
EaeI y'GGCC_r
Cuts at: 0 1290 1965 1973 2201 2241
Size: 1290 675 8 228 40
Fragments arranged by size:
1290 675 228 40 8
EarI CTCTTCn'nnn_
Cuts at: 0 131 467 746 749 2241
Size: 131 336 279 3 1492
Fragments arranged by size:
1492 336 279 131 3
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1312 2241
Size: 1312 929
EcoICRI GAG'CTC
Cuts at: 0 1812 2241
Size: 1812 429
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 415 770 2116 2241
Size: 196 219 355 1346 125
Fragments arranged by size:
1346 355 219 196 125
EcoO109I rG'GnC_Cy
Cuts at: 0 42 1897 2058 2073 2241
Size: 42 1855 161 15 168
Fragments arranged by size:
1855 168 161 42 15
EcoRI G'AATT_C
Cuts at: 0 1411 2241
Size: 1411 830
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 72 142 264 903 1559 2172 2241
Size: 72 70 122 639 656 613 69
Fragments arranged by size:
656 639 613 122 72 70 69
HaeII r_GCGC'y
Cuts at: 0 768 1926 2241
Size: 768 1158 315
Fragments arranged by size:
1158 768 315
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 250 282 1165 1197 2241
Size: 250 32 883 32 1044
Fragments arranged by size:
1044 883 250 32 32
HincII GTy'rAC
Cuts at: 0 687 2241
Size: 687 1554
HindIII A'AGCT_T
Cuts at: 0 2177 2241
Size: 2177 64
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1049 1208 1915 2241
Size: 1049 159 707 326
Fragments arranged by size:
1049 707 326 159
MscI TGG'CCA
Cuts at: 0 1292 1967 1975 2203 2241
Size: 1292 675 8 228 38
Fragments arranged by size:
1292 675 228 38 8
MspA1I CmG'CkG
Cuts at: 0 150 1082 1383 1844 2241
Size: 150 932 301 461 397
Fragments arranged by size:
932 461 397 301 150
NcoI C'CATG_G
Cuts at: 0 1869 2241
Size: 1869 372
NsiI A_TGCA'T
Cuts at: 0 1182 2241
Size: 1182 1059
PacI TTA_AT'TAA
Cuts at: 0 1760 2241
Size: 1760 481
PflMI CCAn_nnn'nTGG
Cuts at: 0 2171 2241
Size: 2171 70
PfoI T'CCnGG_A
Cuts at: 0 185 2241
Size: 185 2056
PspOMI G'GGCC_C
Cuts at: 0 1897 1916 2241
Size: 1897 19 325
Fragments arranged by size:
1897 325 19
PstI C_TGCA'G
Cuts at: 0 974 1240 2241
Size: 974 266 1001
Fragments arranged by size:
1001 974 266
PvuII CAG'CTG
Cuts at: 0 150 1082 1383 1844 2241
Size: 150 932 301 461 397
Fragments arranged by size:
932 461 397 301 150
SacI G_AGCT'C
Cuts at: 0 1814 2241
Size: 1814 427
SbfI CC_TGCA'GG
Cuts at: 0 974 2241
Size: 974 1267
ScaI AGT'ACT
Cuts at: 0 173 1029 2241
Size: 173 856 1212
Fragments arranged by size:
1212 856 173
SfcI C'TryA_G
Cuts at: 0 970 1236 2241
Size: 970 266 1005
Fragments arranged by size:
1005 970 266
SmlI C'TyrA_G
Cuts at: 0 622 2241
Size: 622 1619
SnaBI TAC'GTA
Cuts at: 0 69 2241
Size: 69 2172
StyI C'CwwG_G
Cuts at: 0 38 1869 1956 2229 2241
Size: 38 1831 87 273 12
Fragments arranged by size:
1831 273 87 38 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 244 1276 2241
Size: 244 1032 965
Fragments arranged by size:
1032 965 244
TatI w'GTAC_w
Cuts at: 0 171 668 1027 2241
Size: 171 497 359 1214
Fragments arranged by size:
1214 497 359 171
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 480 512 729 761 2241
Size: 480 32 217 32 1480
Fragments arranged by size:
1480 480 217 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 794 1957 2241
Size: 794 1163 284
Fragments arranged by size:
1163 794 284
Enzymes that do cut:
AcuI AfeI AflII AlwNI ApaI ApaLI ApoI AseI
AvrII BanI BanII BbsI BbvCI BciVI BclI BfrBI
BglI Bme1580I BpmI Bpu10I BsaAI BsaXI BseRI BseYI
BsgI BsiHKAI BsiWI BsmI Bsp1286I BsrDI BstAPI Bsu36I
BtgI BtsI CspCI DraI DraIII DrdI EaeI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI HaeII Hin4I
HincII HindIII MmeI MscI MspA1I NcoI NsiI PacI
PflMI PfoI PspOMI PstI PvuII SacI SbfI ScaI
SfcI SmlI SnaBI StyI TaqII TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflIII AgeI AhdI
AleI AlfI AloI AscI AsiSI AvaI BaeI BamHI
BbeI BcgI BglII BlpI BmgBI BmrI BmtI BplI
BpuEI BsaI BsaBI BsaHI BsaWI BsiEI BsmBI BspEI
BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstBI
BstEII BstXI BstYI BstZ17I BtgZI ClaI EagI EcoNI
EcoRV FseI FspI FspAI HpaI KasI KpnI MfeI
MluI MslI NaeI NarI NdeI NgoMIV NheI NotI
NruI NspI PasI PciI PmeI PmlI PpiI PpuMI
PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI
SanDI SapI SexAI SfiI SfoI SgfI SgrAI SmaI
SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI
XhoI XmaI XmnI ZraI