(Circular) (Six-base) MAPSORT of: pFN21AA1673.seq Check: 6879 from: 1 to: 6841 pFN21AA1673 6841 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4651 278 Size: 53 83 186 936 3115 2468 Fragments arranged by size: 3115 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4184 1937 Size: 2247 4594 Acc65I G'GTAC_C Cuts at: 4162 4162 Size: 6841 AclI AA'CG_TT Cuts at: 1563 4140 4968 5341 1563 Size: 2577 828 373 3063 Fragments arranged by size: 3063 2577 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3313 4904 6288 6556 1632 Size: 1681 1591 1384 268 1917 Fragments arranged by size: 1917 1681 1591 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6644 AflIII A'CryG_T Cuts at: 1203 5740 6717 1203 Size: 4537 977 1327 Fragments arranged by size: 4537 1327 977 AgeI A'CCGG_T Cuts at: 5672 5672 Size: 6841 AhdI GACnn_n'nnGTC Cuts at: 5570 5570 Size: 6841 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6841 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6841 AlwNI CAG_nnn'CTG Cuts at: 2514 4584 6156 2514 Size: 2070 1572 3199 Fragments arranged by size: 3199 2070 1572 ApaI G_GGCC'C Cuts at: 3973 3973 Size: 6841 ApaLI G'TGCA_C Cuts at: 4898 6054 4898 Size: 1156 5685 ApoI r'AATT_y Cuts at: 1493 2629 3013 3986 4076 4150 4376 4528 6637 Size: 1136 384 973 90 74 226 152 2109 Cuts at: 6637 1493 Size: 1697 Fragments arranged by size: 2109 1697 1136 973 384 226 152 90 74 AseI AT'TA_AT Cuts at: 160 1032 5395 160 Size: 872 4363 1606 Fragments arranged by size: 4363 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6841 AvaI C'yCGr_G Cuts at: 1870 1943 2905 4166 1870 Size: 73 962 1261 4545 Fragments arranged by size: 4545 1261 962 73 BamHI G'GATC_C Cuts at: 4171 4538 4171 Size: 367 6474 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4162 5617 618 Size: 325 217 627 2375 1455 1842 Fragments arranged by size: 2375 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2906 3973 4160 1391 Size: 1515 1067 187 4072 Fragments arranged by size: 4072 1515 1067 187 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3234 928 Size: 971 1335 4535 Fragments arranged by size: 4535 1335 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5032 5066 1903 Size: 34 3095 34 3678 Fragments arranged by size: 3678 3095 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4733 5943 4733 Size: 1210 5631 BclI T'GATC_A Cuts at: 1548 3943 1548 Size: 2395 4446 BfrBI ATG'CAT Cuts at: 3506 3554 3558 3506 Size: 48 4 6789 Fragments arranged by size: 6789 48 4 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5452 136 Size: 107 122 71 631 605 3780 1525 Fragments arranged by size: 3780 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2223 6836 2223 Size: 4613 2228 BlpI GC'TnA_GC Cuts at: 4258 4258 Size: 6841 Bme1580I G_kGCm'C Cuts at: 1790 3877 3973 4902 6058 1790 Size: 2087 96 929 1156 2573 Fragments arranged by size: 2573 2087 1156 929 96 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6841 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2055 2460 4572 Size: 416 53 83 399 24 638 405 2112 Cuts at: 4572 5530 442 Size: 958 1753 Fragments arranged by size: 2112 1753 958 638 416 405 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6841 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2181 2400 2920 3023 3182 3378 5501 1239 Size: 942 219 520 103 159 196 2123 2579 Fragments arranged by size: 2579 2123 942 520 219 196 159 103 Bpu10I CC'TnA_GC Cuts at: 3801 3801 Size: 6841 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4966 5831 6129 4966 Size: 865 298 5678 Fragments arranged by size: 5678 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2763 5504 882 Size: 617 1264 2741 2219 Fragments arranged by size: 2741 2219 1264 617 BsaAI yAC'GTr Cuts at: 493 1204 4632 493 Size: 711 3428 2702 Fragments arranged by size: 3428 2702 711 BsaBI GATnn'nnATC Cuts at: 6835 6835 Size: 6841 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4648 5030 5665 275 Size: 53 83 186 936 3115 382 635 1451 Fragments arranged by size: 3115 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3127 5274 5672 5946 6093 6504 3127 Size: 2147 398 274 147 411 3464 Fragments arranged by size: 3464 2147 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2016 2046 1178 Size: 30 165 30 613 30 5973 Fragments arranged by size: 5973 613 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2530 2664 2881 1186 Size: 1344 134 217 5146 Fragments arranged by size: 5146 1344 217 134 BseYI C'CCAG_C Cuts at: 2120 2171 2928 3056 3782 3824 4044 6044 6540 Size: 51 757 128 726 42 220 2000 496 Cuts at: 6540 2120 Size: 2421 Fragments arranged by size: 2421 2000 757 726 496 220 128 51 42 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3557 1163 Size: 573 1821 4447 Fragments arranged by size: 4447 1821 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4291 5052 6080 1540 Size: 275 186 2290 761 1028 2301 Fragments arranged by size: 2301 2290 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2617 4902 4987 6058 2617 Size: 2285 85 1071 3400 Fragments arranged by size: 3400 2285 1071 85 BsmI GAATG_Cn' Cuts at: 3506 4352 4445 3506 Size: 846 93 5902 Fragments arranged by size: 5902 846 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2499 2499 Size: 6841 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2617 2906 3877 3973 4160 4902 4987 Size: 399 827 289 971 96 187 742 85 Cuts at: 4987 6058 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 971 827 742 399 289 187 96 85 BspEI T'CCGG_A Cuts at: 3127 6504 3127 Size: 3377 3464 BspHI T'CATG_A Cuts at: 4730 6460 4730 Size: 1730 5111 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4196 844 Size: 3352 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4728 1394 Size: 429 2905 3507 Fragments arranged by size: 3507 2905 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2392 5336 5510 65 Size: 1183 1144 2944 174 1396 Fragments arranged by size: 2944 1396 1183 1144 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5485 5672 1700 Size: 110 3675 187 2869 Fragments arranged by size: 3675 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6841 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6841 BssSI C'ACGA_G Cuts at: 4062 4901 5913 4062 Size: 839 1012 4990 Fragments arranged by size: 4990 1012 839 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6841 BstBI TT'CG_AA Cuts at: 5645 5645 Size: 6841 BstEII G'GTnAC_C Cuts at: 1184 4106 1184 Size: 2922 3919 BstYI r'GATC_y Cuts at: 1972 2223 4171 4538 4923 4940 6392 6478 6490 Size: 251 1948 367 385 17 1452 86 12 Cuts at: 6490 6836 1972 Size: 346 1977 Fragments arranged by size: 1977 1948 1452 385 367 346 251 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2855 3283 513 Size: 552 939 851 428 4071 Fragments arranged by size: 4071 939 851 552 428 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3758 487 Size: 683 56 2532 3570 Fragments arranged by size: 3570 2532 683 56 BtsI GCAGTG_nn' Cuts at: 2173 3322 4357 5151 5171 2173 Size: 1149 1035 794 20 3843 Fragments arranged by size: 3843 1149 1035 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6841 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6806 DraI TTT'AAA Cuts at: 4145 4145 Size: 6841 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5848 809 Size: 507 4532 1802 Fragments arranged by size: 4532 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3280 4288 5177 Size: 54 1425 50 275 18 1450 1008 889 Cuts at: 5177 6806 8 Size: 1629 43 Fragments arranged by size: 1629 1450 1425 1008 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4288 1537 Size: 275 2476 4090 Fragments arranged by size: 4090 2476 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2899 3303 4771 1353 Size: 1546 404 1468 3423 Fragments arranged by size: 3423 1546 1468 404 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3399 5428 5802 5948 387 Size: 1064 138 1810 2029 374 146 1280 Fragments arranged by size: 2029 1810 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2181 2400 2920 3023 3182 3313 3378 Size: 393 549 219 520 103 159 131 65 Cuts at: 3378 4904 5501 6288 6556 1239 Size: 1526 597 787 268 1524 Fragments arranged by size: 1526 1524 787 597 549 520 393 268 219 159 131 103 65 EcoNI CCTnn'n_nnAGG Cuts at: 3495 4094 3495 Size: 599 6242 EcoO109I rG'GnC_Cy Cuts at: 2587 3969 2587 Size: 1382 5459 EcoRI G'AATT_C Cuts at: 4076 4150 4528 4076 Size: 74 378 6389 Fragments arranged by size: 6389 378 74 FspI TGC'GCA Cuts at: 5347 5347 Size: 6841 HaeII r_GCGC'y Cuts at: 1678 2534 5988 1678 Size: 856 3454 2531 Fragments arranged by size: 3454 2531 856 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2398 2430 2671 2703 3140 Size: 32 135 32 826 32 241 32 437 Cuts at: 3140 3172 3464 3496 3515 3547 3739 3771 5482 Size: 32 292 32 19 32 192 32 1711 Cuts at: 5482 5514 5556 5588 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1711 826 437 292 241 192 135 42 32 32 32 32 32 32 32 32 32 32 19 HincII GTy'rAC Cuts at: 669 1657 1938 2424 4185 4431 669 Size: 988 281 486 1761 246 3079 Fragments arranged by size: 3079 1761 988 486 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6841 HpaI GTT'AAC Cuts at: 4431 4431 Size: 6841 KpnI G_GTAC'C Cuts at: 4166 4166 Size: 6841 MfeI C'AATT_G Cuts at: 4440 4440 Size: 6841 MluI A'CGCG_T Cuts at: 6717 6717 Size: 6841 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5955 6139 1305 Size: 4650 184 2007 Fragments arranged by size: 4650 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5019 Fragments arranged by size: 5019 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2109 2796 3339 4062 4799 5158 5317 Size: 648 943 687 543 723 737 359 159 Cuts at: 5317 518 Size: 2042 Fragments arranged by size: 2042 943 737 723 687 648 543 359 159 MspA1I CmG'CkG Cuts at: 1606 2153 2815 3889 3949 4134 4257 4934 6082 Size: 547 662 1074 60 185 123 677 1148 Cuts at: 6082 6327 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 1074 677 662 547 245 185 123 60 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5350 Fragments arranged by size: 5350 939 552 NdeI CA'TA_TG Cuts at: 387 3220 3931 387 Size: 2833 711 3297 Fragments arranged by size: 3297 2833 711 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6841 NotI GC'GGCC_GC Cuts at: 4288 4288 Size: 6841 NsiI A_TGCA'T Cuts at: 3508 3556 3560 3508 Size: 48 4 6789 Fragments arranged by size: 6789 48 4 NspI r_CATG'y Cuts at: 1132 1229 3154 3558 4199 5744 1132 Size: 97 1925 404 641 1545 2229 Fragments arranged by size: 2229 1925 1545 641 404 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6841 PciI A'CATG_T Cuts at: 5740 5740 Size: 6841 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6841 PfoI T'CCnGG_A Cuts at: 2897 3080 2897 Size: 183 6658 PmeI GTTT'AAAC Cuts at: 4145 4145 Size: 6841 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2981 3013 4910 4942 6423 6455 2981 Size: 32 1897 32 1481 32 3367 Fragments arranged by size: 3367 1897 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2587 2587 Size: 6841 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6841 PsiI TTA'TAA Cuts at: 2138 4411 2138 Size: 2273 4568 PspOMI G'GGCC_C Cuts at: 3969 3969 Size: 6841 PstI C_TGCA'G Cuts at: 830 2091 3894 4026 4193 830 Size: 1261 1803 132 167 3478 Fragments arranged by size: 3478 1803 1261 167 132 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6841 PvuII CAG'CTG Cuts at: 2153 2815 3889 3949 2153 Size: 662 1074 60 5045 Fragments arranged by size: 5045 1074 662 60 SalI G'TCGA_C Cuts at: 1936 4183 1936 Size: 2247 4594 SanDI GG'GwC_CC Cuts at: 2587 2587 Size: 6841 SbfI CC_TGCA'GG Cuts at: 4193 4193 Size: 6841 ScaI AGT'ACT Cuts at: 5089 6801 5089 Size: 1712 5129 SfcI C'TryA_G Cuts at: 826 1045 2087 3890 4022 4189 5324 6005 6196 Size: 219 1042 1803 132 167 1135 681 191 Cuts at: 6196 826 Size: 1471 Fragments arranged by size: 1803 1471 1135 1042 681 219 191 167 132 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6841 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6841 SmaI CCC'GGG Cuts at: 1872 2907 4168 1872 Size: 1035 1261 4545 Fragments arranged by size: 4545 1261 1035 SmlI C'TyrA_G Cuts at: 820 1017 1943 4945 5846 6108 820 Size: 197 926 3002 901 262 1553 Fragments arranged by size: 3002 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4632 493 Size: 4139 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6841 SphI G_CATG'C Cuts at: 1132 3558 4199 1132 Size: 2426 641 3774 Fragments arranged by size: 3774 2426 641 SrfI GCCC'GGGC Cuts at: 2907 2907 Size: 6841 SspI AAT'ATT Cuts at: 5 52 4040 4765 5 Size: 47 3988 725 2081 Fragments arranged by size: 3988 2081 725 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6841 StyI C'CwwG_G Cuts at: 513 1065 2004 2941 3632 4280 513 Size: 552 939 937 691 648 3074 Fragments arranged by size: 3074 939 937 691 648 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2718 4868 4885 5038 5223 5668 325 Size: 1255 1138 2150 17 153 185 445 1498 Fragments arranged by size: 2150 1498 1255 1138 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5087 6799 96 Size: 274 80 33 51 1443 3110 1712 138 Fragments arranged by size: 3110 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2016 2048 3628 3660 6801 6833 2016 Size: 32 1580 32 3141 32 2024 Fragments arranged by size: 3141 2024 1580 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6841 XbaI T'CTAG_A Cuts at: 4177 4177 Size: 6841 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4064 4064 Size: 6841 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6841 XmaI C'CCGG_G Cuts at: 1870 2905 4166 1870 Size: 1035 1261 4545 Fragments arranged by size: 4545 1261 1035 XmnI GAAnn'nnTTC Cuts at: 4970 4970 Size: 6841 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4649 276 Size: 53 83 186 936 3115 2468 Fragments arranged by size: 3115 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SwaI